Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF616 and GFF617 are separated by 175 nucleotides GFF617 and GFF618 overlap by 4 nucleotides GFF618 and GFF619 are separated by 21 nucleotides GFF619 and GFF620 are separated by 54 nucleotides
GFF616 - hypothetical protein, at 664,665 to 665,018
GFF616
GFF617 - no description, at 665,194 to 665,487
GFF617
GFF618 - Lactoylglutathione lyase and related lyases, at 665,484 to 665,834
GFF618
GFF619 - Aspartate carbamoyltransferase (EC 2.1.3.2), at 665,856 to 667,154
GFF619
GFF620 - Transcriptional regulator, LysR family, at 667,209 to 668,213
GFF620
Position (kb) 665
666
667
668 Strain fitness (log2 ratio) -2
-1
0
1
2 at 665.034 kb on + strand at 665.041 kb on - strand at 665.041 kb on - strand at 665.049 kb on + strand at 665.050 kb on - strand at 665.059 kb on - strand at 665.068 kb on + strand at 665.068 kb on + strand at 665.068 kb on + strand at 665.072 kb on + strand at 665.072 kb on + strand at 665.073 kb on - strand at 665.073 kb on - strand at 665.094 kb on + strand at 665.094 kb on + strand at 665.157 kb on + strand at 665.157 kb on + strand at 665.158 kb on - strand at 665.216 kb on + strand at 665.217 kb on - strand at 665.217 kb on - strand at 665.260 kb on + strand, within GFF617 at 665.261 kb on - strand, within GFF617 at 665.261 kb on - strand, within GFF617 at 665.396 kb on + strand, within GFF617 at 665.547 kb on + strand, within GFF618 at 665.784 kb on + strand, within GFF618 at 666.211 kb on + strand, within GFF619 at 666.211 kb on + strand, within GFF619 at 666.352 kb on + strand, within GFF619 at 666.352 kb on + strand, within GFF619 at 666.352 kb on + strand, within GFF619 at 666.353 kb on - strand, within GFF619 at 666.353 kb on - strand, within GFF619 at 666.353 kb on - strand, within GFF619 at 666.353 kb on - strand, within GFF619 at 666.353 kb on - strand, within GFF619 at 666.394 kb on + strand, within GFF619 at 666.395 kb on - strand, within GFF619 at 666.472 kb on + strand, within GFF619 at 666.472 kb on + strand, within GFF619 at 666.472 kb on + strand, within GFF619 at 666.473 kb on - strand, within GFF619 at 666.473 kb on - strand, within GFF619 at 666.473 kb on - strand, within GFF619 at 666.604 kb on + strand, within GFF619 at 666.605 kb on - strand, within GFF619 at 666.632 kb on - strand, within GFF619 at 666.942 kb on - strand, within GFF619 at 666.943 kb on + strand, within GFF619 at 667.165 kb on + strand at 667.165 kb on + strand at 667.399 kb on + strand, within GFF620 at 667.483 kb on + strand, within GFF620 at 667.483 kb on + strand, within GFF620 at 667.483 kb on + strand, within GFF620 at 667.484 kb on - strand, within GFF620 at 667.484 kb on - strand, within GFF620 at 667.484 kb on - strand, within GFF620 at 667.603 kb on + strand, within GFF620 at 667.603 kb on + strand, within GFF620 at 667.603 kb on + strand, within GFF620 at 667.603 kb on + strand, within GFF620 at 667.604 kb on - strand, within GFF620 at 667.604 kb on - strand, within GFF620 at 667.604 kb on - strand, within GFF620 at 667.604 kb on - strand, within GFF620 at 667.881 kb on + strand, within GFF620 at 667.881 kb on + strand, within GFF620 at 667.882 kb on - strand, within GFF620 at 667.882 kb on - strand, within GFF620 at 667.882 kb on - strand, within GFF620
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 665,034 + +0.7 665,041 - -0.5 665,041 - +0.8 665,049 + +0.1 665,050 - +0.2 665,059 - +0.6 665,068 + -0.6 665,068 + +0.1 665,068 + +0.1 665,072 + -0.3 665,072 + +0.2 665,073 - -2.5 665,073 - -0.2 665,094 + +0.0 665,094 + +0.2 665,157 + +0.1 665,157 + -1.0 665,158 - +1.8 665,216 + -1.1 665,217 - -0.1 665,217 - -0.6 665,260 + GFF617 0.22 -0.7 665,261 - GFF617 0.23 -0.7 665,261 - GFF617 0.23 -0.0 665,396 + GFF617 0.69 -0.4 665,547 + GFF618 0.18 -0.1 665,784 + GFF618 0.85 -0.2 666,211 + GFF619 0.27 +0.5 666,211 + GFF619 0.27 -0.3 666,352 + GFF619 0.38 -1.9 666,352 + GFF619 0.38 +0.4 666,352 + GFF619 0.38 +1.5 666,353 - GFF619 0.38 +1.5 666,353 - GFF619 0.38 -1.1 666,353 - GFF619 0.38 -0.4 666,353 - GFF619 0.38 -1.6 666,353 - GFF619 0.38 -0.1 666,394 + GFF619 0.41 +1.5 666,395 - GFF619 0.41 -0.6 666,472 + GFF619 0.47 +0.9 666,472 + GFF619 0.47 +1.0 666,472 + GFF619 0.47 +0.1 666,473 - GFF619 0.47 -0.4 666,473 - GFF619 0.47 -0.5 666,473 - GFF619 0.47 +0.3 666,604 + GFF619 0.58 +0.8 666,605 - GFF619 0.58 +0.6 666,632 - GFF619 0.60 +1.2 666,942 - GFF619 0.84 +0.2 666,943 + GFF619 0.84 +0.4 667,165 + -0.3 667,165 + -0.4 667,399 + GFF620 0.19 -0.9 667,483 + GFF620 0.27 +0.9 667,483 + GFF620 0.27 -0.4 667,483 + GFF620 0.27 -0.1 667,484 - GFF620 0.27 +1.2 667,484 - GFF620 0.27 +0.3 667,484 - GFF620 0.27 +1.5 667,603 + GFF620 0.39 +0.5 667,603 + GFF620 0.39 -0.6 667,603 + GFF620 0.39 -0.7 667,603 + GFF620 0.39 -0.2 667,604 - GFF620 0.39 -1.6 667,604 - GFF620 0.39 -1.1 667,604 - GFF620 0.39 -0.7 667,604 - GFF620 0.39 -0.4 667,881 + GFF620 0.67 -0.2 667,881 + GFF620 0.67 -0.6 667,882 - GFF620 0.67 -0.7 667,882 - GFF620 0.67 -0.2 667,882 - GFF620 0.67 +0.3
Or see this region's nucleotide sequence