Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6110 and GFF6111 are separated by 133 nucleotides GFF6111 and GFF6112 overlap by 4 nucleotides
GFF6110 - N-acyl-D-amino-acid deacylase (EC 3.5.1.81), at 8,570 to 10,033
GFF6110
GFF6111 - Oligopeptide ABC transporter, ATP-binding protein OppF (TC 3.A.1.5.1), at 10,167 to 11,165
GFF6111
GFF6112 - Oligopeptide ABC transporter, ATP-binding protein OppD (TC 3.A.1.5.1), at 11,162 to 12,193
GFF6112
Position (kb)
10
11
12 Strain fitness (log2 ratio)
-2
-1
0
1 at 9.177 kb on + strand, within GFF6110 at 9.177 kb on + strand, within GFF6110 at 9.177 kb on + strand, within GFF6110 at 9.177 kb on + strand, within GFF6110 at 9.178 kb on - strand, within GFF6110 at 9.178 kb on - strand, within GFF6110 at 9.178 kb on - strand, within GFF6110 at 9.178 kb on - strand, within GFF6110 at 9.178 kb on - strand, within GFF6110 at 9.184 kb on - strand, within GFF6110 at 9.381 kb on + strand, within GFF6110 at 9.382 kb on - strand, within GFF6110 at 9.382 kb on - strand, within GFF6110 at 9.382 kb on - strand, within GFF6110 at 9.382 kb on - strand, within GFF6110 at 9.382 kb on - strand, within GFF6110 at 9.457 kb on - strand, within GFF6110 at 9.832 kb on + strand, within GFF6110 at 9.832 kb on + strand, within GFF6110 at 9.832 kb on + strand, within GFF6110 at 9.833 kb on - strand, within GFF6110 at 9.833 kb on - strand, within GFF6110 at 9.945 kb on - strand at 9.945 kb on - strand at 10.011 kb on + strand at 10.012 kb on - strand at 10.080 kb on - strand at 10.080 kb on - strand at 10.394 kb on - strand, within GFF6111 at 10.394 kb on - strand, within GFF6111 at 10.456 kb on + strand, within GFF6111 at 10.456 kb on + strand, within GFF6111 at 10.457 kb on - strand, within GFF6111 at 10.457 kb on - strand, within GFF6111 at 10.520 kb on - strand, within GFF6111 at 10.846 kb on + strand, within GFF6111 at 10.969 kb on - strand, within GFF6111 at 11.110 kb on + strand at 11.111 kb on - strand at 11.111 kb on - strand at 11.111 kb on - strand at 11.346 kb on - strand, within GFF6112 at 11.409 kb on + strand, within GFF6112 at 11.409 kb on + strand, within GFF6112 at 11.410 kb on - strand, within GFF6112 at 11.472 kb on + strand, within GFF6112 at 11.472 kb on + strand, within GFF6112 at 11.472 kb on + strand, within GFF6112 at 11.472 kb on + strand, within GFF6112 at 11.472 kb on + strand, within GFF6112 at 11.472 kb on + strand, within GFF6112 at 11.472 kb on + strand, within GFF6112 at 11.473 kb on - strand, within GFF6112 at 11.473 kb on - strand, within GFF6112 at 11.473 kb on - strand, within GFF6112 at 11.473 kb on - strand, within GFF6112 at 11.706 kb on + strand, within GFF6112 at 11.807 kb on + strand, within GFF6112 at 11.807 kb on + strand, within GFF6112 at 12.073 kb on - strand, within GFF6112
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 9,177 + GFF6110 0.41 +0.9 9,177 + GFF6110 0.41 -1.4 9,177 + GFF6110 0.41 -0.6 9,177 + GFF6110 0.41 -0.7 9,178 - GFF6110 0.42 -0.6 9,178 - GFF6110 0.42 -1.7 9,178 - GFF6110 0.42 +1.4 9,178 - GFF6110 0.42 +0.4 9,178 - GFF6110 0.42 -1.4 9,184 - GFF6110 0.42 -0.5 9,381 + GFF6110 0.55 -0.4 9,382 - GFF6110 0.55 -0.5 9,382 - GFF6110 0.55 +0.9 9,382 - GFF6110 0.55 -0.3 9,382 - GFF6110 0.55 +0.4 9,382 - GFF6110 0.55 -0.5 9,457 - GFF6110 0.61 +1.1 9,832 + GFF6110 0.86 -1.1 9,832 + GFF6110 0.86 -0.2 9,832 + GFF6110 0.86 -1.4 9,833 - GFF6110 0.86 -0.4 9,833 - GFF6110 0.86 -0.1 9,945 - -0.9 9,945 - -0.1 10,011 + -0.8 10,012 - +0.1 10,080 - +0.9 10,080 - -0.5 10,394 - GFF6111 0.23 -0.3 10,394 - GFF6111 0.23 +0.7 10,456 + GFF6111 0.29 -1.6 10,456 + GFF6111 0.29 -0.9 10,457 - GFF6111 0.29 -0.2 10,457 - GFF6111 0.29 -0.8 10,520 - GFF6111 0.35 -0.0 10,846 + GFF6111 0.68 -1.5 10,969 - GFF6111 0.80 -0.3 11,110 + -0.2 11,111 - +0.3 11,111 - -0.3 11,111 - -1.0 11,346 - GFF6112 0.18 +0.8 11,409 + GFF6112 0.24 -0.1 11,409 + GFF6112 0.24 -0.8 11,410 - GFF6112 0.24 +0.1 11,472 + GFF6112 0.30 -1.8 11,472 + GFF6112 0.30 -0.3 11,472 + GFF6112 0.30 -0.4 11,472 + GFF6112 0.30 -0.3 11,472 + GFF6112 0.30 +0.1 11,472 + GFF6112 0.30 +1.3 11,472 + GFF6112 0.30 -1.4 11,473 - GFF6112 0.30 -0.6 11,473 - GFF6112 0.30 -0.7 11,473 - GFF6112 0.30 -1.1 11,473 - GFF6112 0.30 +0.6 11,706 + GFF6112 0.53 -1.6 11,807 + GFF6112 0.62 -0.0 11,807 + GFF6112 0.62 -0.7 12,073 - GFF6112 0.88 -0.5
Or see this region's nucleotide sequence