Strain Fitness in Variovorax sp. SCN45 around GFF6091

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF6089 and GFF6090 are separated by 3 nucleotidesGFF6090 and GFF6091 overlap by 4 nucleotidesGFF6091 and GFF6092 are separated by 28 nucleotides GFF6089 - 1,2-epoxyphenylacetyl-CoA isomerase (EC 5.3.3.18), at 150,137 to 150,940 GFF6089 GFF6090 - Acyl-coenzyme A thioesterase PaaD (Pse.pu.) (E. coli PaaI), at 150,944 to 151,402 GFF6090 GFF6091 - Phenylacetate-coenzyme A ligase (EC 6.2.1.30), at 151,399 to 152,709 GFF6091 GFF6092 - Penicillin G acylase precursor (EC 3.5.1.11), at 152,738 to 155,182 GFF6092 Position (kb) 151 152 153Strain fitness (log2 ratio) -2 -1 0 1at 150.410 kb on + strand, within GFF6089at 150.410 kb on + strand, within GFF6089at 150.410 kb on + strand, within GFF6089at 150.410 kb on + strand, within GFF6089at 150.410 kb on + strand, within GFF6089at 150.411 kb on - strand, within GFF6089at 150.411 kb on - strand, within GFF6089at 150.411 kb on - strand, within GFF6089at 150.413 kb on + strand, within GFF6089at 150.413 kb on + strand, within GFF6089at 150.413 kb on + strand, within GFF6089at 150.413 kb on + strand, within GFF6089at 150.413 kb on + strand, within GFF6089at 150.413 kb on + strand, within GFF6089at 150.413 kb on + strand, within GFF6089at 150.414 kb on - strand, within GFF6089at 150.414 kb on - strand, within GFF6089at 150.414 kb on - strand, within GFF6089at 150.414 kb on - strand, within GFF6089at 150.414 kb on - strand, within GFF6089at 150.414 kb on - strand, within GFF6089at 150.983 kb on + strandat 150.983 kb on + strandat 150.983 kb on + strandat 150.984 kb on - strandat 151.176 kb on - strand, within GFF6090at 151.176 kb on - strand, within GFF6090at 151.176 kb on - strand, within GFF6090at 151.292 kb on + strand, within GFF6090at 151.292 kb on + strand, within GFF6090at 151.292 kb on + strand, within GFF6090at 151.292 kb on + strand, within GFF6090at 151.292 kb on + strand, within GFF6090at 151.293 kb on - strand, within GFF6090at 151.293 kb on - strand, within GFF6090at 151.293 kb on - strand, within GFF6090at 151.293 kb on - strand, within GFF6090at 151.293 kb on - strand, within GFF6090at 151.293 kb on - strand, within GFF6090at 151.293 kb on - strand, within GFF6090at 151.352 kb on + strand, within GFF6090at 151.528 kb on + strandat 151.633 kb on + strand, within GFF6091at 151.633 kb on + strand, within GFF6091at 151.633 kb on + strand, within GFF6091at 151.633 kb on + strand, within GFF6091at 151.633 kb on + strand, within GFF6091at 151.634 kb on - strand, within GFF6091at 151.679 kb on + strand, within GFF6091at 151.680 kb on - strand, within GFF6091at 151.723 kb on + strand, within GFF6091at 151.817 kb on - strand, within GFF6091at 151.817 kb on - strand, within GFF6091at 151.822 kb on + strand, within GFF6091at 151.856 kb on - strand, within GFF6091at 151.969 kb on + strand, within GFF6091at 151.969 kb on + strand, within GFF6091at 151.969 kb on + strand, within GFF6091at 151.969 kb on + strand, within GFF6091at 151.970 kb on - strand, within GFF6091at 151.970 kb on - strand, within GFF6091at 151.970 kb on - strand, within GFF6091at 151.970 kb on - strand, within GFF6091at 151.970 kb on - strand, within GFF6091at 151.970 kb on - strand, within GFF6091at 152.119 kb on + strand, within GFF6091at 152.119 kb on + strand, within GFF6091at 152.119 kb on + strand, within GFF6091at 152.119 kb on + strand, within GFF6091at 152.120 kb on - strand, within GFF6091at 152.206 kb on + strand, within GFF6091at 152.207 kb on - strand, within GFF6091at 152.367 kb on + strand, within GFF6091at 152.479 kb on + strand, within GFF6091at 152.479 kb on + strand, within GFF6091at 152.480 kb on - strand, within GFF6091at 152.480 kb on - strand, within GFF6091at 152.867 kb on + strandat 152.868 kb on - strandat 152.868 kb on - strandat 152.868 kb on - strandat 152.930 kb on + strandat 152.931 kb on - strandat 152.993 kb on + strand, within GFF6092at 152.994 kb on - strand, within GFF6092at 152.994 kb on - strand, within GFF6092at 152.994 kb on - strand, within GFF6092at 153.206 kb on + strand, within GFF6092at 153.206 kb on + strand, within GFF6092at 153.207 kb on - strand, within GFF6092at 153.248 kb on + strand, within GFF6092at 153.249 kb on - strand, within GFF6092at 153.632 kb on + strand, within GFF6092at 153.632 kb on + strand, within GFF6092at 153.632 kb on + strand, within GFF6092at 153.632 kb on + strand, within GFF6092at 153.633 kb on - strand, within GFF6092at 153.633 kb on - strand, within GFF6092

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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150,410 + GFF6089 0.34 +0.5
150,410 + GFF6089 0.34 -0.5
150,410 + GFF6089 0.34 -2.0
150,410 + GFF6089 0.34 -0.1
150,410 + GFF6089 0.34 +0.4
150,411 - GFF6089 0.34 -0.7
150,411 - GFF6089 0.34 +1.4
150,411 - GFF6089 0.34 +0.2
150,413 + GFF6089 0.34 -1.0
150,413 + GFF6089 0.34 +1.5
150,413 + GFF6089 0.34 +0.5
150,413 + GFF6089 0.34 -0.0
150,413 + GFF6089 0.34 +1.3
150,413 + GFF6089 0.34 -0.9
150,413 + GFF6089 0.34 -0.5
150,414 - GFF6089 0.34 +0.2
150,414 - GFF6089 0.34 +0.1
150,414 - GFF6089 0.34 -0.0
150,414 - GFF6089 0.34 -0.5
150,414 - GFF6089 0.34 -0.5
150,414 - GFF6089 0.34 -0.8
150,983 + -0.5
150,983 + +0.2
150,983 + +0.5
150,984 - -0.5
151,176 - GFF6090 0.51 -1.1
151,176 - GFF6090 0.51 -0.8
151,176 - GFF6090 0.51 -1.0
151,292 + GFF6090 0.76 -1.0
151,292 + GFF6090 0.76 -1.3
151,292 + GFF6090 0.76 +1.3
151,292 + GFF6090 0.76 -2.5
151,292 + GFF6090 0.76 +0.4
151,293 - GFF6090 0.76 -1.0
151,293 - GFF6090 0.76 -0.4
151,293 - GFF6090 0.76 +0.1
151,293 - GFF6090 0.76 -1.9
151,293 - GFF6090 0.76 -1.0
151,293 - GFF6090 0.76 -0.3
151,293 - GFF6090 0.76 -1.5
151,352 + GFF6090 0.89 -0.1
151,528 + +0.1
151,633 + GFF6091 0.18 +0.4
151,633 + GFF6091 0.18 -0.2
151,633 + GFF6091 0.18 +0.3
151,633 + GFF6091 0.18 -0.1
151,633 + GFF6091 0.18 +0.5
151,634 - GFF6091 0.18 -0.4
151,679 + GFF6091 0.21 -0.4
151,680 - GFF6091 0.21 -0.3
151,723 + GFF6091 0.25 -0.1
151,817 - GFF6091 0.32 +0.3
151,817 - GFF6091 0.32 +0.8
151,822 + GFF6091 0.32 -0.2
151,856 - GFF6091 0.35 -0.4
151,969 + GFF6091 0.43 -0.1
151,969 + GFF6091 0.43 -0.1
151,969 + GFF6091 0.43 -0.5
151,969 + GFF6091 0.43 -0.3
151,970 - GFF6091 0.44 -0.3
151,970 - GFF6091 0.44 +0.4
151,970 - GFF6091 0.44 -1.0
151,970 - GFF6091 0.44 -0.4
151,970 - GFF6091 0.44 -1.5
151,970 - GFF6091 0.44 -0.6
152,119 + GFF6091 0.55 -0.9
152,119 + GFF6091 0.55 -0.8
152,119 + GFF6091 0.55 -1.4
152,119 + GFF6091 0.55 +0.1
152,120 - GFF6091 0.55 +0.1
152,206 + GFF6091 0.62 -0.8
152,207 - GFF6091 0.62 -2.3
152,367 + GFF6091 0.74 -0.5
152,479 + GFF6091 0.82 -0.3
152,479 + GFF6091 0.82 -0.7
152,480 - GFF6091 0.82 +1.0
152,480 - GFF6091 0.82 +1.0
152,867 + -0.3
152,868 - -0.2
152,868 - -1.6
152,868 - +0.2
152,930 + +0.1
152,931 - -0.2
152,993 + GFF6092 0.10 +1.5
152,994 - GFF6092 0.10 -0.0
152,994 - GFF6092 0.10 -0.7
152,994 - GFF6092 0.10 -0.8
153,206 + GFF6092 0.19 +0.8
153,206 + GFF6092 0.19 -0.7
153,207 - GFF6092 0.19 -0.4
153,248 + GFF6092 0.21 +0.2
153,249 - GFF6092 0.21 -0.4
153,632 + GFF6092 0.37 -0.0
153,632 + GFF6092 0.37 +0.4
153,632 + GFF6092 0.37 -1.1
153,632 + GFF6092 0.37 -0.0
153,633 - GFF6092 0.37 -0.5
153,633 - GFF6092 0.37 -0.5

Or see this region's nucleotide sequence