Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6013 and GFF6014 are separated by 110 nucleotides GFF6014 and GFF6015 are separated by 13 nucleotides GFF6015 and GFF6016 are separated by 85 nucleotides
GFF6013 - Muconate cycloisomerase (EC 5.5.1.1), at 73,073 to 74,227
GFF6013
GFF6014 - Transcriptional regulator, LysR family, at 74,338 to 75,225
GFF6014
GFF6015 - Transcriptional regulator PA0877, LysR family, at 75,239 to 76,129
GFF6015
GFF6016 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 76,215 to 77,144
GFF6016
Position (kb) 74
75
76 Strain fitness (log2 ratio) -1
0
1 at 73.620 kb on + strand, within GFF6013 at 73.620 kb on + strand, within GFF6013 at 73.620 kb on + strand, within GFF6013 at 73.621 kb on - strand, within GFF6013 at 73.621 kb on - strand, within GFF6013 at 73.834 kb on - strand, within GFF6013 at 73.947 kb on + strand, within GFF6013 at 73.980 kb on + strand, within GFF6013 at 74.467 kb on + strand, within GFF6014 at 74.468 kb on - strand, within GFF6014 at 74.468 kb on - strand, within GFF6014 at 74.495 kb on + strand, within GFF6014 at 74.495 kb on + strand, within GFF6014 at 74.495 kb on + strand, within GFF6014 at 74.496 kb on - strand, within GFF6014 at 74.742 kb on - strand, within GFF6014 at 75.804 kb on + strand, within GFF6015 at 75.804 kb on + strand, within GFF6015 at 75.805 kb on - strand, within GFF6015 at 76.086 kb on + strand at 76.086 kb on + strand at 76.087 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 73,620 + GFF6013 0.47 -0.5 73,620 + GFF6013 0.47 -0.1 73,620 + GFF6013 0.47 -0.9 73,621 - GFF6013 0.47 -0.1 73,621 - GFF6013 0.47 -0.8 73,834 - GFF6013 0.66 -0.3 73,947 + GFF6013 0.76 -1.0 73,980 + GFF6013 0.79 +0.1 74,467 + GFF6014 0.15 +0.5 74,468 - GFF6014 0.15 +0.4 74,468 - GFF6014 0.15 +0.0 74,495 + GFF6014 0.18 -0.2 74,495 + GFF6014 0.18 -0.3 74,495 + GFF6014 0.18 +0.5 74,496 - GFF6014 0.18 +0.5 74,742 - GFF6014 0.45 +0.5 75,804 + GFF6015 0.63 -0.2 75,804 + GFF6015 0.63 -0.7 75,805 - GFF6015 0.64 +0.2 76,086 + -0.0 76,086 + -1.5 76,087 - -1.6
Or see this region's nucleotide sequence