Strain Fitness in Variovorax sp. SCN45 around GFF6013

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF6012 and GFF6013 are separated by 90 nucleotidesGFF6013 and GFF6014 are separated by 110 nucleotides GFF6012 - Tricarboxylate transport protein TctC, at 71,978 to 72,982 GFF6012 GFF6013 - Muconate cycloisomerase (EC 5.5.1.1), at 73,073 to 74,227 GFF6013 GFF6014 - Transcriptional regulator, LysR family, at 74,338 to 75,225 GFF6014 Position (kb) 73 74 75Strain fitness (log2 ratio) -1 0 1at 72.075 kb on + strandat 72.178 kb on + strand, within GFF6012at 72.489 kb on + strand, within GFF6012at 72.873 kb on + strand, within GFF6012at 73.237 kb on - strand, within GFF6013at 73.243 kb on - strand, within GFF6013at 73.620 kb on + strand, within GFF6013at 73.620 kb on + strand, within GFF6013at 73.620 kb on + strand, within GFF6013at 73.621 kb on - strand, within GFF6013at 73.621 kb on - strand, within GFF6013at 73.834 kb on - strand, within GFF6013at 73.947 kb on + strand, within GFF6013at 73.980 kb on + strand, within GFF6013at 74.467 kb on + strand, within GFF6014at 74.468 kb on - strand, within GFF6014at 74.468 kb on - strand, within GFF6014at 74.495 kb on + strand, within GFF6014at 74.495 kb on + strand, within GFF6014at 74.495 kb on + strand, within GFF6014at 74.496 kb on - strand, within GFF6014at 74.742 kb on - strand, within GFF6014

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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72,075 + -0.5
72,178 + GFF6012 0.20 +0.5
72,489 + GFF6012 0.51 -0.7
72,873 + GFF6012 0.89 +0.7
73,237 - GFF6013 0.14 -1.2
73,243 - GFF6013 0.15 -0.4
73,620 + GFF6013 0.47 -0.5
73,620 + GFF6013 0.47 -0.1
73,620 + GFF6013 0.47 -0.9
73,621 - GFF6013 0.47 -0.1
73,621 - GFF6013 0.47 -0.8
73,834 - GFF6013 0.66 -0.3
73,947 + GFF6013 0.76 -1.0
73,980 + GFF6013 0.79 +0.1
74,467 + GFF6014 0.15 +0.5
74,468 - GFF6014 0.15 +0.4
74,468 - GFF6014 0.15 +0.0
74,495 + GFF6014 0.18 -0.2
74,495 + GFF6014 0.18 -0.3
74,495 + GFF6014 0.18 +0.5
74,496 - GFF6014 0.18 +0.5
74,742 - GFF6014 0.45 +0.5

Or see this region's nucleotide sequence