Strain Fitness in Variovorax sp. SCN45 around GFF6010

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF6009 and GFF6010 are separated by 10 nucleotidesGFF6010 and GFF6011 are separated by 6 nucleotidesGFF6011 and GFF6012 are separated by 111 nucleotides GFF6009 - Acyl-CoA dehydrogenase STM0857, at 68,617 to 69,780 GFF6009 GFF6010 - MmgE/PrpD family protein, at 69,791 to 71,152 GFF6010 GFF6011 - putative decarboxylase, at 71,159 to 71,866 GFF6011 GFF6012 - Tricarboxylate transport protein TctC, at 71,978 to 72,982 GFF6012 Position (kb) 69 70 71 72Strain fitness (log2 ratio) -2 -1 0 1at 68.887 kb on + strand, within GFF6009at 68.887 kb on + strand, within GFF6009at 68.887 kb on + strand, within GFF6009at 68.887 kb on + strand, within GFF6009at 68.888 kb on - strand, within GFF6009at 68.888 kb on - strand, within GFF6009at 68.888 kb on - strand, within GFF6009at 68.938 kb on + strand, within GFF6009at 69.055 kb on + strand, within GFF6009at 69.325 kb on + strand, within GFF6009at 69.325 kb on + strand, within GFF6009at 69.325 kb on + strand, within GFF6009at 69.326 kb on - strand, within GFF6009at 69.326 kb on - strand, within GFF6009at 69.416 kb on - strand, within GFF6009at 69.524 kb on - strand, within GFF6009at 69.586 kb on + strand, within GFF6009at 69.586 kb on + strand, within GFF6009at 69.587 kb on - strand, within GFF6009at 69.701 kb on - strandat 69.701 kb on - strandat 69.739 kb on + strandat 69.739 kb on + strandat 69.743 kb on + strandat 69.744 kb on - strandat 69.999 kb on + strand, within GFF6010at 70.000 kb on - strand, within GFF6010at 70.000 kb on - strand, within GFF6010at 70.000 kb on - strand, within GFF6010at 70.242 kb on - strand, within GFF6010at 70.242 kb on - strand, within GFF6010at 70.598 kb on + strand, within GFF6010at 70.805 kb on + strand, within GFF6010at 70.805 kb on + strand, within GFF6010at 70.914 kb on - strand, within GFF6010at 71.057 kb on + strandat 71.057 kb on + strandat 71.058 kb on - strandat 71.122 kb on + strandat 71.463 kb on + strand, within GFF6011at 71.497 kb on - strand, within GFF6011at 71.522 kb on + strand, within GFF6011at 71.523 kb on - strand, within GFF6011at 71.731 kb on - strand, within GFF6011at 71.873 kb on - strandat 71.998 kb on + strandat 71.998 kb on + strandat 71.998 kb on + strandat 71.999 kb on - strandat 72.075 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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68,887 + GFF6009 0.23 -1.1
68,887 + GFF6009 0.23 -0.6
68,887 + GFF6009 0.23 -0.7
68,887 + GFF6009 0.23 -1.0
68,888 - GFF6009 0.23 +0.5
68,888 - GFF6009 0.23 -0.4
68,888 - GFF6009 0.23 -0.1
68,938 + GFF6009 0.28 +1.5
69,055 + GFF6009 0.38 +0.2
69,325 + GFF6009 0.61 -1.8
69,325 + GFF6009 0.61 +0.7
69,325 + GFF6009 0.61 -0.2
69,326 - GFF6009 0.61 +0.1
69,326 - GFF6009 0.61 -0.1
69,416 - GFF6009 0.69 +0.1
69,524 - GFF6009 0.78 -0.4
69,586 + GFF6009 0.83 -2.5
69,586 + GFF6009 0.83 -1.0
69,587 - GFF6009 0.83 -0.1
69,701 - -0.8
69,701 - -0.1
69,739 + -0.3
69,739 + -0.0
69,743 + -0.3
69,744 - -0.0
69,999 + GFF6010 0.15 -0.5
70,000 - GFF6010 0.15 -0.9
70,000 - GFF6010 0.15 -0.6
70,000 - GFF6010 0.15 -1.3
70,242 - GFF6010 0.33 -0.3
70,242 - GFF6010 0.33 +0.7
70,598 + GFF6010 0.59 -0.9
70,805 + GFF6010 0.74 -1.4
70,805 + GFF6010 0.74 -0.9
70,914 - GFF6010 0.82 +1.0
71,057 + -0.4
71,057 + -0.1
71,058 - -0.3
71,122 + -0.5
71,463 + GFF6011 0.43 -0.9
71,497 - GFF6011 0.48 -0.3
71,522 + GFF6011 0.51 -0.2
71,523 - GFF6011 0.51 +0.2
71,731 - GFF6011 0.81 +0.1
71,873 - -0.5
71,998 + +0.7
71,998 + +0.1
71,998 + -0.3
71,999 - -1.5
72,075 + -0.5

Or see this region's nucleotide sequence