Strain Fitness in Variovorax sp. SCN45 around GFF5950

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5948 and GFF5949 are separated by 3 nucleotidesGFF5949 and GFF5950 overlap by 4 nucleotidesGFF5950 and GFF5951 are separated by 1 nucleotidesGFF5951 and GFF5952 are separated by 60 nucleotides GFF5948 - hypothetical protein, at 6,143 to 6,790 GFF5948 GFF5949 - Glyoxalase family protein, at 6,794 to 7,207 GFF5949 GFF5950 - no description, at 7,204 to 7,797 GFF5950 GFF5951 - no description, at 7,799 to 8,236 GFF5951 GFF5952 - no description, at 8,297 to 8,665 GFF5952 Position (kb) 7 8Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 6.329 kb on + strand, within GFF5948at 6.344 kb on + strand, within GFF5948at 6.345 kb on - strand, within GFF5948at 6.359 kb on + strand, within GFF5948at 6.452 kb on + strand, within GFF5948at 6.452 kb on + strand, within GFF5948at 6.452 kb on + strand, within GFF5948at 6.453 kb on - strand, within GFF5948at 6.453 kb on - strand, within GFF5948at 6.453 kb on - strand, within GFF5948at 6.453 kb on - strand, within GFF5948at 6.453 kb on - strand, within GFF5948at 6.455 kb on - strand, within GFF5948at 6.455 kb on - strand, within GFF5948at 6.500 kb on + strand, within GFF5948at 6.501 kb on - strand, within GFF5948at 6.501 kb on - strand, within GFF5948at 6.531 kb on - strand, within GFF5948at 6.716 kb on + strand, within GFF5948at 6.717 kb on - strand, within GFF5948at 6.717 kb on - strand, within GFF5948at 7.358 kb on - strand, within GFF5950at 7.456 kb on + strand, within GFF5950at 7.456 kb on + strand, within GFF5950at 7.456 kb on + strand, within GFF5950at 7.456 kb on + strand, within GFF5950at 7.457 kb on - strand, within GFF5950at 7.457 kb on - strand, within GFF5950at 7.457 kb on - strand, within GFF5950at 7.528 kb on + strand, within GFF5950at 7.528 kb on + strand, within GFF5950at 7.529 kb on - strand, within GFF5950at 7.529 kb on - strand, within GFF5950at 7.612 kb on + strand, within GFF5950at 7.612 kb on + strand, within GFF5950at 7.613 kb on - strand, within GFF5950at 7.718 kb on - strand, within GFF5950at 7.718 kb on - strand, within GFF5950at 7.730 kb on - strand, within GFF5950at 7.735 kb on + strand, within GFF5950at 7.735 kb on + strand, within GFF5950at 7.736 kb on - strand, within GFF5950at 7.736 kb on - strand, within GFF5950at 7.736 kb on - strand, within GFF5950at 7.736 kb on - strand, within GFF5950at 7.741 kb on + strandat 7.741 kb on + strandat 7.741 kb on + strandat 7.742 kb on - strandat 7.762 kb on + strandat 7.762 kb on + strandat 7.763 kb on - strandat 7.976 kb on + strand, within GFF5951at 7.976 kb on + strand, within GFF5951at 7.985 kb on + strand, within GFF5951at 7.986 kb on - strand, within GFF5951at 7.986 kb on - strand, within GFF5951at 8.066 kb on + strand, within GFF5951at 8.066 kb on + strand, within GFF5951at 8.067 kb on - strand, within GFF5951at 8.067 kb on - strand, within GFF5951at 8.115 kb on - strand, within GFF5951at 8.144 kb on + strand, within GFF5951at 8.144 kb on + strand, within GFF5951at 8.144 kb on + strand, within GFF5951at 8.144 kb on + strand, within GFF5951at 8.145 kb on - strand, within GFF5951at 8.157 kb on - strand, within GFF5951at 8.157 kb on - strand, within GFF5951at 8.184 kb on - strand, within GFF5951at 8.184 kb on - strand, within GFF5951at 8.184 kb on - strand, within GFF5951at 8.184 kb on - strand, within GFF5951at 8.184 kb on - strand, within GFF5951at 8.184 kb on - strand, within GFF5951at 8.372 kb on + strand, within GFF5952

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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6,329 + GFF5948 0.29 +1.5
6,344 + GFF5948 0.31 -1.5
6,345 - GFF5948 0.31 -0.3
6,359 + GFF5948 0.33 -1.5
6,452 + GFF5948 0.48 +0.5
6,452 + GFF5948 0.48 -0.2
6,452 + GFF5948 0.48 -1.0
6,453 - GFF5948 0.48 +0.2
6,453 - GFF5948 0.48 -0.1
6,453 - GFF5948 0.48 +0.1
6,453 - GFF5948 0.48 -0.5
6,453 - GFF5948 0.48 +0.1
6,455 - GFF5948 0.48 -0.8
6,455 - GFF5948 0.48 +4.1
6,500 + GFF5948 0.55 -0.0
6,501 - GFF5948 0.55 -0.1
6,501 - GFF5948 0.55 -0.3
6,531 - GFF5948 0.60 -1.2
6,716 + GFF5948 0.88 -0.5
6,717 - GFF5948 0.89 -0.5
6,717 - GFF5948 0.89 -1.8
7,358 - GFF5950 0.26 -1.5
7,456 + GFF5950 0.42 +0.5
7,456 + GFF5950 0.42 -0.5
7,456 + GFF5950 0.42 +0.4
7,456 + GFF5950 0.42 +0.4
7,457 - GFF5950 0.43 -1.8
7,457 - GFF5950 0.43 -0.0
7,457 - GFF5950 0.43 +0.5
7,528 + GFF5950 0.55 +1.5
7,528 + GFF5950 0.55 -0.2
7,529 - GFF5950 0.55 -0.5
7,529 - GFF5950 0.55 +0.7
7,612 + GFF5950 0.69 +0.1
7,612 + GFF5950 0.69 -0.3
7,613 - GFF5950 0.69 +0.4
7,718 - GFF5950 0.87 +1.7
7,718 - GFF5950 0.87 +0.2
7,730 - GFF5950 0.89 -0.7
7,735 + GFF5950 0.89 -0.7
7,735 + GFF5950 0.89 -0.9
7,736 - GFF5950 0.90 +0.7
7,736 - GFF5950 0.90 +0.3
7,736 - GFF5950 0.90 +1.0
7,736 - GFF5950 0.90 +0.2
7,741 + -0.6
7,741 + +1.2
7,741 + -0.6
7,742 - -0.2
7,762 + -0.7
7,762 + -0.3
7,763 - -0.2
7,976 + GFF5951 0.40 -0.3
7,976 + GFF5951 0.40 -0.4
7,985 + GFF5951 0.42 -0.0
7,986 - GFF5951 0.43 -1.5
7,986 - GFF5951 0.43 +0.8
8,066 + GFF5951 0.61 +0.9
8,066 + GFF5951 0.61 -0.2
8,067 - GFF5951 0.61 +0.7
8,067 - GFF5951 0.61 -0.2
8,115 - GFF5951 0.72 +0.1
8,144 + GFF5951 0.79 -0.6
8,144 + GFF5951 0.79 +0.1
8,144 + GFF5951 0.79 -0.5
8,144 + GFF5951 0.79 +1.4
8,145 - GFF5951 0.79 -0.5
8,157 - GFF5951 0.82 -0.4
8,157 - GFF5951 0.82 -0.5
8,184 - GFF5951 0.88 -1.3
8,184 - GFF5951 0.88 -1.3
8,184 - GFF5951 0.88 +0.3
8,184 - GFF5951 0.88 -1.3
8,184 - GFF5951 0.88 +0.7
8,184 - GFF5951 0.88 -0.4
8,372 + GFF5952 0.20 +0.4

Or see this region's nucleotide sequence