Strain Fitness in Variovorax sp. SCN45 around GFF5776

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5775 and GFF5776 overlap by 41 nucleotidesGFF5776 and GFF5777 are separated by 4 nucleotidesGFF5777 and GFF5778 are separated by 154 nucleotides GFF5775 - Glucoamylase (EC 3.2.1.3), at 149,391 to 151,298 GFF5775 GFF5776 - Gluconokinase (EC 2.7.1.12), at 151,258 to 151,803 GFF5776 GFF5777 - 5-keto-D-gluconate 5-reductase (EC 1.1.1.69), at 151,808 to 152,569 GFF5777 GFF5778 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 152,724 to 153,677 GFF5778 Position (kb) 151 152Strain fitness (log2 ratio) -2 -1 0 1at 150.265 kb on - strand, within GFF5775at 150.286 kb on - strand, within GFF5775at 150.286 kb on - strand, within GFF5775at 150.363 kb on + strand, within GFF5775at 150.363 kb on + strand, within GFF5775at 150.363 kb on + strand, within GFF5775at 150.364 kb on - strand, within GFF5775at 150.364 kb on - strand, within GFF5775at 150.364 kb on - strand, within GFF5775at 150.420 kb on + strand, within GFF5775at 150.421 kb on - strand, within GFF5775at 150.483 kb on + strand, within GFF5775at 150.780 kb on + strand, within GFF5775at 150.780 kb on + strand, within GFF5775at 150.810 kb on + strand, within GFF5775at 150.813 kb on + strand, within GFF5775at 150.813 kb on + strand, within GFF5775at 150.814 kb on - strand, within GFF5775at 150.928 kb on - strand, within GFF5775at 150.942 kb on + strand, within GFF5775at 150.942 kb on + strand, within GFF5775at 151.029 kb on + strand, within GFF5775at 151.029 kb on + strand, within GFF5775at 151.029 kb on + strand, within GFF5775at 151.029 kb on + strand, within GFF5775at 151.029 kb on + strand, within GFF5775at 151.029 kb on + strand, within GFF5775at 151.030 kb on - strand, within GFF5775at 151.030 kb on - strand, within GFF5775at 151.030 kb on - strand, within GFF5775at 151.119 kb on + strandat 151.120 kb on - strandat 151.120 kb on - strandat 151.120 kb on - strandat 151.357 kb on + strand, within GFF5776at 151.357 kb on + strand, within GFF5776at 151.358 kb on - strand, within GFF5776at 151.358 kb on - strand, within GFF5776at 151.358 kb on - strand, within GFF5776at 151.408 kb on + strand, within GFF5776at 151.427 kb on + strand, within GFF5776at 151.427 kb on + strand, within GFF5776at 151.427 kb on + strand, within GFF5776at 151.427 kb on + strand, within GFF5776at 151.427 kb on + strand, within GFF5776at 151.428 kb on - strand, within GFF5776at 151.428 kb on - strand, within GFF5776at 151.809 kb on + strandat 151.810 kb on - strandat 151.810 kb on - strandat 151.839 kb on + strandat 151.839 kb on + strandat 151.839 kb on + strandat 151.839 kb on + strandat 151.840 kb on - strandat 151.840 kb on - strandat 151.840 kb on - strandat 151.846 kb on + strandat 152.004 kb on + strand, within GFF5777at 152.005 kb on - strand, within GFF5777at 152.005 kb on - strand, within GFF5777at 152.037 kb on + strand, within GFF5777at 152.038 kb on - strand, within GFF5777at 152.038 kb on - strand, within GFF5777at 152.038 kb on - strand, within GFF5777at 152.038 kb on - strand, within GFF5777at 152.101 kb on - strand, within GFF5777at 152.101 kb on - strand, within GFF5777at 152.667 kb on + strandat 152.667 kb on + strandat 152.668 kb on - strandat 152.668 kb on - strandat 152.668 kb on - strandat 152.668 kb on - strandat 152.668 kb on - strandat 152.770 kb on - strandat 152.770 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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150,265 - GFF5775 0.46 -0.6
150,286 - GFF5775 0.47 -1.5
150,286 - GFF5775 0.47 +0.2
150,363 + GFF5775 0.51 +0.2
150,363 + GFF5775 0.51 -0.8
150,363 + GFF5775 0.51 -1.2
150,364 - GFF5775 0.51 -1.1
150,364 - GFF5775 0.51 -0.5
150,364 - GFF5775 0.51 -1.5
150,420 + GFF5775 0.54 -1.2
150,421 - GFF5775 0.54 -0.4
150,483 + GFF5775 0.57 +1.2
150,780 + GFF5775 0.73 +0.1
150,780 + GFF5775 0.73 +0.6
150,810 + GFF5775 0.74 +0.1
150,813 + GFF5775 0.75 +0.9
150,813 + GFF5775 0.75 -0.5
150,814 - GFF5775 0.75 +0.4
150,928 - GFF5775 0.81 -2.1
150,942 + GFF5775 0.81 -0.2
150,942 + GFF5775 0.81 -1.5
151,029 + GFF5775 0.86 -0.9
151,029 + GFF5775 0.86 -0.2
151,029 + GFF5775 0.86 -0.9
151,029 + GFF5775 0.86 -1.8
151,029 + GFF5775 0.86 +0.1
151,029 + GFF5775 0.86 -0.6
151,030 - GFF5775 0.86 -1.4
151,030 - GFF5775 0.86 +0.6
151,030 - GFF5775 0.86 -0.8
151,119 + -0.3
151,120 - -0.1
151,120 - -0.8
151,120 - -0.1
151,357 + GFF5776 0.18 +0.6
151,357 + GFF5776 0.18 -0.6
151,358 - GFF5776 0.18 -1.2
151,358 - GFF5776 0.18 -0.8
151,358 - GFF5776 0.18 +1.1
151,408 + GFF5776 0.27 +0.9
151,427 + GFF5776 0.31 +1.2
151,427 + GFF5776 0.31 -0.7
151,427 + GFF5776 0.31 +1.1
151,427 + GFF5776 0.31 -0.0
151,427 + GFF5776 0.31 -0.1
151,428 - GFF5776 0.31 +0.0
151,428 - GFF5776 0.31 -0.2
151,809 + -0.3
151,810 - +0.0
151,810 - -0.0
151,839 + +0.4
151,839 + +1.1
151,839 + +0.1
151,839 + +0.5
151,840 - -0.6
151,840 - -0.4
151,840 - +1.3
151,846 + -0.3
152,004 + GFF5777 0.26 +0.5
152,005 - GFF5777 0.26 -1.2
152,005 - GFF5777 0.26 +0.8
152,037 + GFF5777 0.30 -0.3
152,038 - GFF5777 0.30 +0.9
152,038 - GFF5777 0.30 -0.1
152,038 - GFF5777 0.30 +0.5
152,038 - GFF5777 0.30 +0.2
152,101 - GFF5777 0.38 -0.0
152,101 - GFF5777 0.38 +0.2
152,667 + -0.9
152,667 + +0.4
152,668 - +0.6
152,668 - -0.2
152,668 - +0.5
152,668 - +0.2
152,668 - -0.9
152,770 - +0.1
152,770 - +1.1

Or see this region's nucleotide sequence