Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF573 and GFF574 overlap by 4 nucleotides GFF574 and GFF575 overlap by 4 nucleotides GFF575 and GFF576 are separated by 15 nucleotides GFF576 and GFF577 are separated by 3 nucleotides
GFF573 - Valine--pyruvate aminotransferase (EC 2.6.1.66), at 618,549 to 619,745
GFF573
GFF574 - Tol-Pal system-associated acyl-CoA thioesterase, at 619,742 to 620,200
GFF574
GFF575 - Tol-Pal system protein TolQ, at 620,197 to 620,919
GFF575
GFF576 - Tol biopolymer transport system, TolR protein, at 620,935 to 621,372
GFF576
GFF577 - TolA protein, at 621,376 to 622,299
GFF577
Position (kb) 620
621 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 619.260 kb on + strand, within GFF573 at 619.260 kb on + strand, within GFF573 at 619.260 kb on + strand, within GFF573 at 619.260 kb on + strand, within GFF573 at 619.260 kb on + strand, within GFF573 at 619.260 kb on + strand, within GFF573 at 619.260 kb on + strand, within GFF573 at 619.260 kb on + strand, within GFF573 at 619.260 kb on + strand, within GFF573 at 619.261 kb on - strand, within GFF573 at 619.261 kb on - strand, within GFF573 at 619.261 kb on - strand, within GFF573 at 619.261 kb on - strand, within GFF573 at 619.261 kb on - strand, within GFF573 at 619.261 kb on - strand, within GFF573 at 619.261 kb on - strand, within GFF573 at 619.261 kb on - strand, within GFF573 at 619.315 kb on + strand, within GFF573 at 619.374 kb on + strand, within GFF573 at 619.416 kb on + strand, within GFF573 at 619.417 kb on - strand, within GFF573 at 619.417 kb on - strand, within GFF573 at 619.492 kb on + strand, within GFF573 at 619.493 kb on - strand, within GFF573 at 619.518 kb on + strand, within GFF573 at 619.518 kb on + strand, within GFF573 at 619.518 kb on + strand, within GFF573 at 619.518 kb on + strand, within GFF573 at 619.518 kb on + strand, within GFF573 at 619.518 kb on + strand, within GFF573 at 619.518 kb on + strand, within GFF573 at 619.518 kb on + strand, within GFF573 at 619.518 kb on + strand, within GFF573 at 619.518 kb on + strand, within GFF573 at 619.518 kb on + strand, within GFF573 at 619.518 kb on + strand, within GFF573 at 619.518 kb on + strand, within GFF573 at 619.518 kb on + strand, within GFF573 at 619.518 kb on + strand, within GFF573 at 619.518 kb on + strand, within GFF573 at 619.518 kb on + strand, within GFF573 at 619.518 kb on + strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.519 kb on - strand, within GFF573 at 619.748 kb on + strand at 619.748 kb on + strand at 619.749 kb on - strand at 619.749 kb on - strand at 619.749 kb on - strand at 619.749 kb on - strand at 619.749 kb on - strand at 619.770 kb on - strand at 619.805 kb on + strand, within GFF574 at 619.805 kb on + strand, within GFF574 at 619.805 kb on + strand, within GFF574 at 619.805 kb on + strand, within GFF574 at 619.805 kb on + strand, within GFF574 at 619.805 kb on + strand, within GFF574 at 619.805 kb on + strand, within GFF574 at 619.805 kb on + strand, within GFF574 at 619.806 kb on - strand, within GFF574 at 619.806 kb on - strand, within GFF574 at 619.806 kb on - strand, within GFF574 at 619.806 kb on - strand, within GFF574 at 619.806 kb on - strand, within GFF574 at 619.814 kb on + strand, within GFF574 at 619.814 kb on + strand, within GFF574 at 619.814 kb on + strand, within GFF574 at 619.814 kb on + strand, within GFF574 at 619.814 kb on + strand, within GFF574 at 619.814 kb on + strand, within GFF574 at 619.814 kb on + strand, within GFF574 at 619.815 kb on - strand, within GFF574 at 619.815 kb on - strand, within GFF574 at 619.815 kb on - strand, within GFF574 at 619.815 kb on - strand, within GFF574 at 619.815 kb on - strand, within GFF574 at 619.815 kb on - strand, within GFF574 at 619.815 kb on - strand, within GFF574 at 619.815 kb on - strand, within GFF574 at 619.815 kb on - strand, within GFF574 at 619.815 kb on - strand, within GFF574 at 619.815 kb on - strand, within GFF574 at 619.931 kb on + strand, within GFF574 at 619.931 kb on + strand, within GFF574 at 619.931 kb on + strand, within GFF574 at 619.931 kb on + strand, within GFF574 at 619.931 kb on + strand, within GFF574 at 619.931 kb on + strand, within GFF574 at 619.932 kb on - strand, within GFF574 at 619.932 kb on - strand, within GFF574 at 619.932 kb on - strand, within GFF574 at 619.932 kb on - strand, within GFF574 at 619.932 kb on - strand, within GFF574 at 619.932 kb on - strand, within GFF574 at 619.969 kb on + strand, within GFF574 at 619.970 kb on - strand, within GFF574 at 620.008 kb on - strand, within GFF574 at 620.026 kb on - strand, within GFF574 at 620.134 kb on - strand, within GFF574 at 620.165 kb on + strand at 620.165 kb on + strand at 620.165 kb on + strand at 620.166 kb on - strand at 620.166 kb on - strand at 620.166 kb on - strand at 620.166 kb on - strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.189 kb on - strand at 620.189 kb on - strand at 620.189 kb on - strand at 620.189 kb on - strand at 620.189 kb on - strand at 620.189 kb on - strand at 620.189 kb on - strand at 620.189 kb on - strand at 620.189 kb on - strand at 620.189 kb on - strand at 620.189 kb on - strand at 620.189 kb on - strand at 620.189 kb on - strand at 620.210 kb on + strand at 620.210 kb on + strand at 620.210 kb on + strand at 620.210 kb on + strand at 620.210 kb on + strand at 620.210 kb on + strand at 620.210 kb on + strand at 620.210 kb on + strand at 620.210 kb on + strand at 620.210 kb on + strand at 620.211 kb on - strand at 620.211 kb on - strand at 620.211 kb on - strand at 620.211 kb on - strand at 620.211 kb on - strand at 620.211 kb on - strand at 620.211 kb on - strand at 620.211 kb on - strand at 620.211 kb on - strand at 620.211 kb on - strand at 620.211 kb on - strand at 620.211 kb on - strand at 620.211 kb on - strand at 620.211 kb on - strand at 620.314 kb on + strand, within GFF575 at 620.314 kb on + strand, within GFF575 at 620.314 kb on + strand, within GFF575 at 620.314 kb on + strand, within GFF575 at 620.315 kb on - strand, within GFF575 at 620.315 kb on - strand, within GFF575 at 620.468 kb on - strand, within GFF575 at 620.468 kb on - strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.773 kb on + strand, within GFF575 at 620.773 kb on + strand, within GFF575 at 620.774 kb on - strand, within GFF575 at 620.774 kb on - strand, within GFF575 at 620.774 kb on - strand, within GFF575 at 620.824 kb on + strand, within GFF575 at 620.824 kb on + strand, within GFF575 at 620.824 kb on + strand, within GFF575 at 620.824 kb on + strand, within GFF575 at 620.824 kb on + strand, within GFF575 at 620.824 kb on + strand, within GFF575 at 620.824 kb on + strand, within GFF575 at 620.824 kb on + strand, within GFF575 at 620.825 kb on - strand, within GFF575 at 620.825 kb on - strand, within GFF575 at 620.825 kb on - strand, within GFF575 at 620.825 kb on - strand, within GFF575 at 620.825 kb on - strand, within GFF575 at 620.825 kb on - strand, within GFF575 at 620.825 kb on - strand, within GFF575 at 620.825 kb on - strand, within GFF575 at 621.283 kb on + strand, within GFF576 at 621.283 kb on + strand, within GFF576 at 621.283 kb on + strand, within GFF576 at 621.283 kb on + strand, within GFF576 at 621.283 kb on + strand, within GFF576 at 621.284 kb on - strand, within GFF576 at 621.284 kb on - strand, within GFF576 at 621.284 kb on - strand, within GFF576 at 621.370 kb on + strand at 621.370 kb on + strand at 621.370 kb on + strand at 621.370 kb on + strand at 621.370 kb on + strand at 621.370 kb on + strand at 621.370 kb on + strand at 621.370 kb on + strand at 621.370 kb on + strand at 621.371 kb on - strand at 621.371 kb on - strand at 621.371 kb on - strand at 621.371 kb on - strand at 621.371 kb on - strand at 621.371 kb on - strand at 621.371 kb on - strand at 621.371 kb on - strand at 621.371 kb on - strand at 621.371 kb on - strand at 621.371 kb on - strand at 621.371 kb on - strand at 621.602 kb on + strand, within GFF577
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 619,260 + GFF573 0.59 -0.2 619,260 + GFF573 0.59 -0.1 619,260 + GFF573 0.59 +0.7 619,260 + GFF573 0.59 -0.6 619,260 + GFF573 0.59 -0.7 619,260 + GFF573 0.59 +0.4 619,260 + GFF573 0.59 -0.8 619,260 + GFF573 0.59 -0.6 619,260 + GFF573 0.59 -0.5 619,261 - GFF573 0.59 +0.4 619,261 - GFF573 0.59 -0.1 619,261 - GFF573 0.59 -1.3 619,261 - GFF573 0.59 -0.1 619,261 - GFF573 0.59 +0.8 619,261 - GFF573 0.59 +0.5 619,261 - GFF573 0.59 +0.4 619,261 - GFF573 0.59 -0.0 619,315 + GFF573 0.64 -0.7 619,374 + GFF573 0.69 -0.2 619,416 + GFF573 0.72 -0.3 619,417 - GFF573 0.73 -0.4 619,417 - GFF573 0.73 -0.2 619,492 + GFF573 0.79 -0.0 619,493 - GFF573 0.79 -0.2 619,518 + GFF573 0.81 -0.5 619,518 + GFF573 0.81 -0.7 619,518 + GFF573 0.81 -0.1 619,518 + GFF573 0.81 +0.5 619,518 + GFF573 0.81 -1.1 619,518 + GFF573 0.81 -1.5 619,518 + GFF573 0.81 -0.4 619,518 + GFF573 0.81 +0.4 619,518 + GFF573 0.81 -0.3 619,518 + GFF573 0.81 -0.4 619,518 + GFF573 0.81 -1.3 619,518 + GFF573 0.81 +0.5 619,518 + GFF573 0.81 +0.2 619,518 + GFF573 0.81 -0.6 619,518 + GFF573 0.81 -0.1 619,518 + GFF573 0.81 +1.3 619,518 + GFF573 0.81 -1.8 619,518 + GFF573 0.81 -0.2 619,519 - GFF573 0.81 +0.8 619,519 - GFF573 0.81 +0.0 619,519 - GFF573 0.81 -0.2 619,519 - GFF573 0.81 -0.9 619,519 - GFF573 0.81 -0.5 619,519 - GFF573 0.81 -0.2 619,519 - GFF573 0.81 +0.3 619,519 - GFF573 0.81 +0.5 619,519 - GFF573 0.81 -0.6 619,519 - GFF573 0.81 +0.3 619,519 - GFF573 0.81 +0.2 619,519 - GFF573 0.81 +0.2 619,519 - GFF573 0.81 -1.0 619,519 - GFF573 0.81 -0.8 619,519 - GFF573 0.81 -0.6 619,519 - GFF573 0.81 +0.9 619,519 - GFF573 0.81 +0.2 619,519 - GFF573 0.81 -1.3 619,519 - GFF573 0.81 +1.1 619,519 - GFF573 0.81 -0.4 619,519 - GFF573 0.81 +0.2 619,519 - GFF573 0.81 +0.0 619,519 - GFF573 0.81 -1.3 619,519 - GFF573 0.81 +0.3 619,519 - GFF573 0.81 -0.2 619,519 - GFF573 0.81 -0.6 619,519 - GFF573 0.81 -0.5 619,519 - GFF573 0.81 +0.2 619,519 - GFF573 0.81 +0.2 619,519 - GFF573 0.81 -0.1 619,519 - GFF573 0.81 +1.8 619,519 - GFF573 0.81 +0.1 619,519 - GFF573 0.81 +1.0 619,519 - GFF573 0.81 -0.5 619,519 - GFF573 0.81 -0.1 619,748 + -0.4 619,748 + -0.1 619,749 - -0.1 619,749 - +0.2 619,749 - -1.3 619,749 - -1.9 619,749 - -0.3 619,770 - -0.0 619,805 + GFF574 0.14 -0.6 619,805 + GFF574 0.14 -0.2 619,805 + GFF574 0.14 -0.4 619,805 + GFF574 0.14 +0.6 619,805 + GFF574 0.14 -0.1 619,805 + GFF574 0.14 -0.2 619,805 + GFF574 0.14 +0.4 619,805 + GFF574 0.14 -0.2 619,806 - GFF574 0.14 -0.5 619,806 - GFF574 0.14 -0.3 619,806 - GFF574 0.14 -1.8 619,806 - GFF574 0.14 -0.4 619,806 - GFF574 0.14 +0.7 619,814 + GFF574 0.16 +2.2 619,814 + GFF574 0.16 -0.4 619,814 + GFF574 0.16 -0.8 619,814 + GFF574 0.16 -0.0 619,814 + GFF574 0.16 -0.6 619,814 + GFF574 0.16 -0.6 619,814 + GFF574 0.16 +0.8 619,815 - GFF574 0.16 -1.1 619,815 - GFF574 0.16 -0.0 619,815 - GFF574 0.16 -1.0 619,815 - GFF574 0.16 -1.0 619,815 - GFF574 0.16 +0.1 619,815 - GFF574 0.16 +0.1 619,815 - GFF574 0.16 -0.2 619,815 - GFF574 0.16 -0.5 619,815 - GFF574 0.16 -0.6 619,815 - GFF574 0.16 +0.2 619,815 - GFF574 0.16 -0.2 619,931 + GFF574 0.41 +0.9 619,931 + GFF574 0.41 +1.6 619,931 + GFF574 0.41 -0.3 619,931 + GFF574 0.41 +0.2 619,931 + GFF574 0.41 -0.3 619,931 + GFF574 0.41 -0.7 619,932 - GFF574 0.41 -0.5 619,932 - GFF574 0.41 -0.7 619,932 - GFF574 0.41 +1.8 619,932 - GFF574 0.41 +0.5 619,932 - GFF574 0.41 -0.9 619,932 - GFF574 0.41 +1.2 619,969 + GFF574 0.49 -0.6 619,970 - GFF574 0.50 -0.4 620,008 - GFF574 0.58 -0.6 620,026 - GFF574 0.62 +0.3 620,134 - GFF574 0.85 +0.3 620,165 + +0.1 620,165 + -1.4 620,165 + -1.3 620,166 - +1.2 620,166 - +0.2 620,166 - -1.7 620,166 - -0.4 620,188 + -1.1 620,188 + +2.6 620,188 + -1.7 620,188 + -0.2 620,188 + +0.6 620,188 + +0.2 620,188 + -1.3 620,188 + -0.6 620,188 + -0.1 620,188 + -0.2 620,188 + -0.1 620,188 + +0.0 620,188 + -0.0 620,188 + +0.8 620,188 + +0.1 620,188 + -0.2 620,188 + +0.8 620,188 + +0.2 620,188 + +0.5 620,189 - +0.2 620,189 - +0.6 620,189 - +0.0 620,189 - +0.4 620,189 - -1.8 620,189 - -0.7 620,189 - +0.4 620,189 - -1.1 620,189 - -0.1 620,189 - +0.2 620,189 - -0.9 620,189 - +0.1 620,189 - -0.7 620,210 + +0.5 620,210 + -2.5 620,210 + -0.2 620,210 + -1.5 620,210 + -0.5 620,210 + -1.3 620,210 + -0.3 620,210 + -0.6 620,210 + -0.6 620,210 + -1.4 620,211 - -0.0 620,211 - +0.0 620,211 - -0.6 620,211 - -0.5 620,211 - +0.2 620,211 - -1.0 620,211 - -0.5 620,211 - +0.7 620,211 - -0.3 620,211 - -0.4 620,211 - +0.6 620,211 - +0.2 620,211 - -0.2 620,211 - -1.1 620,314 + GFF575 0.16 +1.2 620,314 + GFF575 0.16 -1.1 620,314 + GFF575 0.16 -1.2 620,314 + GFF575 0.16 -2.6 620,315 - GFF575 0.16 -0.0 620,315 - GFF575 0.16 +0.5 620,468 - GFF575 0.37 -0.2 620,468 - GFF575 0.37 -1.2 620,614 + GFF575 0.58 -0.8 620,614 + GFF575 0.58 -3.4 620,614 + GFF575 0.58 -1.4 620,614 + GFF575 0.58 -0.4 620,614 + GFF575 0.58 -0.8 620,614 + GFF575 0.58 -2.1 620,614 + GFF575 0.58 -0.9 620,614 + GFF575 0.58 -0.9 620,614 + GFF575 0.58 -1.1 620,614 + GFF575 0.58 -1.2 620,614 + GFF575 0.58 -1.7 620,614 + GFF575 0.58 -1.2 620,615 - GFF575 0.58 -1.1 620,615 - GFF575 0.58 -0.1 620,615 - GFF575 0.58 -2.0 620,615 - GFF575 0.58 -0.7 620,615 - GFF575 0.58 -2.1 620,615 - GFF575 0.58 -1.1 620,615 - GFF575 0.58 -1.2 620,615 - GFF575 0.58 -1.9 620,615 - GFF575 0.58 -2.2 620,615 - GFF575 0.58 -0.0 620,615 - GFF575 0.58 -0.1 620,615 - GFF575 0.58 +0.1 620,615 - GFF575 0.58 -1.4 620,615 - GFF575 0.58 -2.0 620,615 - GFF575 0.58 +0.2 620,615 - GFF575 0.58 -0.9 620,615 - GFF575 0.58 -1.8 620,615 - GFF575 0.58 -3.4 620,615 - GFF575 0.58 -1.2 620,773 + GFF575 0.80 -0.9 620,773 + GFF575 0.80 -0.2 620,774 - GFF575 0.80 -0.0 620,774 - GFF575 0.80 -0.7 620,774 - GFF575 0.80 -0.8 620,824 + GFF575 0.87 -1.1 620,824 + GFF575 0.87 -1.8 620,824 + GFF575 0.87 -1.0 620,824 + GFF575 0.87 -0.9 620,824 + GFF575 0.87 -0.8 620,824 + GFF575 0.87 -0.2 620,824 + GFF575 0.87 -0.4 620,824 + GFF575 0.87 -1.8 620,825 - GFF575 0.87 -1.0 620,825 - GFF575 0.87 -2.8 620,825 - GFF575 0.87 -1.9 620,825 - GFF575 0.87 -0.6 620,825 - GFF575 0.87 -2.1 620,825 - GFF575 0.87 -1.1 620,825 - GFF575 0.87 -0.5 620,825 - GFF575 0.87 -1.6 621,283 + GFF576 0.79 -1.4 621,283 + GFF576 0.79 -1.8 621,283 + GFF576 0.79 +1.2 621,283 + GFF576 0.79 -0.8 621,283 + GFF576 0.79 -0.8 621,284 - GFF576 0.80 -1.9 621,284 - GFF576 0.80 +0.3 621,284 - GFF576 0.80 -2.5 621,370 + -0.1 621,370 + -0.1 621,370 + -0.6 621,370 + -0.3 621,370 + -0.1 621,370 + -1.6 621,370 + -0.6 621,370 + -0.9 621,370 + +0.3 621,371 - -0.6 621,371 - -0.2 621,371 - -0.6 621,371 - -1.4 621,371 - -0.6 621,371 - +0.1 621,371 - +1.2 621,371 - -0.0 621,371 - -0.3 621,371 - -0.6 621,371 - -0.5 621,371 - -0.6 621,602 + GFF577 0.24 +0.2
Or see this region's nucleotide sequence