Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5705 and GFF5706 are separated by 3 nucleotides GFF5706 and GFF5707 are separated by 146 nucleotides GFF5707 and GFF5708 are separated by 80 nucleotides
GFF5705 - Transcriptional regulator, AcrR family, at 79,456 to 80,031
GFF5705
GFF5706 - no description, at 80,035 to 80,187
GFF5706
GFF5707 - Transcriptional regulator, LysR family, at 80,334 to 81,191
GFF5707
GFF5708 - Pyridoxal phosphate-dependent aminotransferase, at 81,272 to 82,606
GFF5708
Position (kb)
80
81
82 Strain fitness (log2 ratio)
-1
0
1
2 at 79.444 kb on + strand at 79.444 kb on + strand at 79.444 kb on + strand at 79.444 kb on + strand at 79.444 kb on + strand at 79.445 kb on - strand at 79.445 kb on - strand at 79.445 kb on - strand at 79.445 kb on - strand at 79.482 kb on - strand at 79.482 kb on - strand at 79.539 kb on + strand, within GFF5705 at 79.539 kb on + strand, within GFF5705 at 79.539 kb on + strand, within GFF5705 at 79.539 kb on + strand, within GFF5705 at 79.539 kb on + strand, within GFF5705 at 79.539 kb on + strand, within GFF5705 at 79.539 kb on + strand, within GFF5705 at 79.540 kb on - strand, within GFF5705 at 79.540 kb on - strand, within GFF5705 at 79.540 kb on - strand, within GFF5705 at 79.540 kb on - strand, within GFF5705 at 79.540 kb on - strand, within GFF5705 at 79.540 kb on - strand, within GFF5705 at 79.573 kb on + strand, within GFF5705 at 79.573 kb on + strand, within GFF5705 at 79.573 kb on + strand, within GFF5705 at 79.574 kb on - strand, within GFF5705 at 79.574 kb on - strand, within GFF5705 at 79.601 kb on - strand, within GFF5705 at 79.621 kb on + strand, within GFF5705 at 79.621 kb on + strand, within GFF5705 at 79.621 kb on + strand, within GFF5705 at 79.621 kb on + strand, within GFF5705 at 79.621 kb on + strand, within GFF5705 at 79.622 kb on - strand, within GFF5705 at 79.622 kb on - strand, within GFF5705 at 79.622 kb on - strand, within GFF5705 at 79.622 kb on - strand, within GFF5705 at 79.622 kb on - strand, within GFF5705 at 79.622 kb on - strand, within GFF5705 at 79.834 kb on + strand, within GFF5705 at 79.835 kb on - strand, within GFF5705 at 79.835 kb on - strand, within GFF5705 at 79.835 kb on - strand, within GFF5705 at 80.144 kb on + strand, within GFF5706 at 80.144 kb on + strand, within GFF5706 at 80.144 kb on + strand, within GFF5706 at 80.144 kb on - strand, within GFF5706 at 80.145 kb on - strand, within GFF5706 at 80.145 kb on - strand, within GFF5706 at 80.153 kb on + strand, within GFF5706 at 80.269 kb on - strand at 80.328 kb on + strand at 80.328 kb on + strand at 80.328 kb on + strand at 80.329 kb on - strand at 80.329 kb on - strand at 80.329 kb on - strand at 80.560 kb on + strand, within GFF5707 at 80.826 kb on + strand, within GFF5707 at 80.864 kb on - strand, within GFF5707 at 81.034 kb on + strand, within GFF5707 at 81.209 kb on + strand at 81.209 kb on + strand at 81.210 kb on - strand at 81.225 kb on + strand at 81.226 kb on - strand at 81.348 kb on - strand at 81.402 kb on - strand at 81.402 kb on - strand at 81.402 kb on - strand at 81.402 kb on - strand at 81.737 kb on + strand, within GFF5708 at 81.737 kb on + strand, within GFF5708 at 81.737 kb on + strand, within GFF5708 at 81.737 kb on + strand, within GFF5708 at 81.737 kb on + strand, within GFF5708 at 81.737 kb on + strand, within GFF5708 at 81.737 kb on + strand, within GFF5708 at 81.737 kb on + strand, within GFF5708 at 81.738 kb on - strand, within GFF5708 at 81.738 kb on - strand, within GFF5708 at 81.738 kb on - strand, within GFF5708 at 81.738 kb on - strand, within GFF5708 at 81.738 kb on - strand, within GFF5708 at 81.738 kb on - strand, within GFF5708 at 81.738 kb on - strand, within GFF5708 at 81.738 kb on - strand, within GFF5708 at 81.758 kb on + strand, within GFF5708 at 81.965 kb on + strand, within GFF5708 at 81.966 kb on - strand, within GFF5708 at 81.966 kb on - strand, within GFF5708
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 79,444 + +0.1 79,444 + -0.3 79,444 + -0.9 79,444 + +1.6 79,444 + +0.1 79,445 - +0.1 79,445 - -0.5 79,445 - +0.2 79,445 - +0.0 79,482 - +0.6 79,482 - -1.1 79,539 + GFF5705 0.14 -0.5 79,539 + GFF5705 0.14 -0.2 79,539 + GFF5705 0.14 -0.1 79,539 + GFF5705 0.14 +1.2 79,539 + GFF5705 0.14 -0.7 79,539 + GFF5705 0.14 +0.4 79,539 + GFF5705 0.14 -0.5 79,540 - GFF5705 0.15 -0.6 79,540 - GFF5705 0.15 -0.7 79,540 - GFF5705 0.15 -0.6 79,540 - GFF5705 0.15 -0.3 79,540 - GFF5705 0.15 +0.8 79,540 - GFF5705 0.15 -0.9 79,573 + GFF5705 0.20 +0.5 79,573 + GFF5705 0.20 +0.8 79,573 + GFF5705 0.20 -0.2 79,574 - GFF5705 0.20 -1.4 79,574 - GFF5705 0.20 +0.1 79,601 - GFF5705 0.25 -0.6 79,621 + GFF5705 0.29 +0.4 79,621 + GFF5705 0.29 +1.1 79,621 + GFF5705 0.29 -1.3 79,621 + GFF5705 0.29 -0.1 79,621 + GFF5705 0.29 +0.3 79,622 - GFF5705 0.29 -0.8 79,622 - GFF5705 0.29 -0.2 79,622 - GFF5705 0.29 -0.6 79,622 - GFF5705 0.29 -0.2 79,622 - GFF5705 0.29 +1.2 79,622 - GFF5705 0.29 -0.9 79,834 + GFF5705 0.66 +0.0 79,835 - GFF5705 0.66 +0.1 79,835 - GFF5705 0.66 +0.2 79,835 - GFF5705 0.66 +0.5 80,144 + GFF5706 0.71 -0.1 80,144 + GFF5706 0.71 -0.9 80,144 + GFF5706 0.71 +0.8 80,144 - GFF5706 0.71 +0.9 80,145 - GFF5706 0.72 -0.4 80,145 - GFF5706 0.72 -0.7 80,153 + GFF5706 0.77 +0.1 80,269 - -0.1 80,328 + +1.6 80,328 + -0.8 80,328 + -0.5 80,329 - -0.3 80,329 - +0.4 80,329 - -1.7 80,560 + GFF5707 0.26 -0.7 80,826 + GFF5707 0.57 +0.5 80,864 - GFF5707 0.62 +1.1 81,034 + GFF5707 0.82 -0.1 81,209 + -0.9 81,209 + -0.0 81,210 - +0.5 81,225 + +1.1 81,226 - -1.4 81,348 - +0.6 81,402 - +0.8 81,402 - -0.4 81,402 - -0.7 81,402 - -0.1 81,737 + GFF5708 0.35 -0.6 81,737 + GFF5708 0.35 -0.6 81,737 + GFF5708 0.35 -0.5 81,737 + GFF5708 0.35 +1.6 81,737 + GFF5708 0.35 -0.5 81,737 + GFF5708 0.35 -0.7 81,737 + GFF5708 0.35 -0.9 81,737 + GFF5708 0.35 -0.1 81,738 - GFF5708 0.35 +0.6 81,738 - GFF5708 0.35 +0.6 81,738 - GFF5708 0.35 +0.2 81,738 - GFF5708 0.35 -0.9 81,738 - GFF5708 0.35 -0.4 81,738 - GFF5708 0.35 -0.2 81,738 - GFF5708 0.35 +2.4 81,738 - GFF5708 0.35 -0.1 81,758 + GFF5708 0.36 -0.4 81,965 + GFF5708 0.52 +0.5 81,966 - GFF5708 0.52 +0.8 81,966 - GFF5708 0.52 -0.4
Or see this region's nucleotide sequence