Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5698 and GFF5699 are separated by 60 nucleotides GFF5699 and GFF5700 overlap by 1 nucleotides GFF5700 and GFF5701 are separated by 108 nucleotides
GFF5698 - 2',3'-cyclic-nucleotide 2'-phosphodiesterase (EC 3.1.4.16) / 3'-nucleotidase (EC 3.1.3.6), at 71,894 to 73,996
GFF5698
GFF5699 - Smr domain protein, at 74,057 to 74,713
GFF5699
GFF5700 - UPF0758 family protein, at 74,713 to 75,390
GFF5700
GFF5701 - FKBP-type peptidyl-prolyl cis-trans isomerase SlpA (EC 5.2.1.8), at 75,499 to 75,942
GFF5701
Position (kb)
74
75 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 73.136 kb on + strand, within GFF5698 at 73.136 kb on + strand, within GFF5698 at 73.136 kb on + strand, within GFF5698 at 73.136 kb on + strand, within GFF5698 at 73.136 kb on + strand, within GFF5698 at 73.137 kb on - strand, within GFF5698 at 73.137 kb on - strand, within GFF5698 at 73.137 kb on - strand, within GFF5698 at 73.137 kb on - strand, within GFF5698 at 73.181 kb on + strand, within GFF5698 at 73.181 kb on + strand, within GFF5698 at 73.181 kb on + strand, within GFF5698 at 73.182 kb on - strand, within GFF5698 at 73.182 kb on - strand, within GFF5698 at 73.182 kb on - strand, within GFF5698 at 73.241 kb on + strand, within GFF5698 at 73.242 kb on - strand, within GFF5698 at 73.242 kb on - strand, within GFF5698 at 73.242 kb on - strand, within GFF5698 at 73.242 kb on - strand, within GFF5698 at 73.253 kb on + strand, within GFF5698 at 73.254 kb on - strand, within GFF5698 at 73.254 kb on - strand, within GFF5698 at 73.254 kb on - strand, within GFF5698 at 73.277 kb on + strand, within GFF5698 at 73.326 kb on - strand, within GFF5698 at 73.343 kb on + strand, within GFF5698 at 73.343 kb on + strand, within GFF5698 at 73.394 kb on + strand, within GFF5698 at 73.394 kb on + strand, within GFF5698 at 73.395 kb on - strand, within GFF5698 at 73.395 kb on - strand, within GFF5698 at 73.395 kb on - strand, within GFF5698 at 73.395 kb on - strand, within GFF5698 at 73.415 kb on + strand, within GFF5698 at 73.415 kb on + strand, within GFF5698 at 73.535 kb on + strand, within GFF5698 at 73.536 kb on - strand, within GFF5698 at 73.571 kb on + strand, within GFF5698 at 73.571 kb on + strand, within GFF5698 at 73.571 kb on + strand, within GFF5698 at 73.571 kb on + strand, within GFF5698 at 73.622 kb on + strand, within GFF5698 at 73.622 kb on + strand, within GFF5698 at 73.622 kb on + strand, within GFF5698 at 73.623 kb on - strand, within GFF5698 at 73.652 kb on + strand, within GFF5698 at 73.676 kb on + strand, within GFF5698 at 73.677 kb on - strand, within GFF5698 at 73.734 kb on - strand, within GFF5698 at 73.734 kb on - strand, within GFF5698 at 73.773 kb on - strand, within GFF5698 at 73.773 kb on - strand, within GFF5698 at 73.773 kb on - strand, within GFF5698 at 73.967 kb on + strand at 73.968 kb on - strand at 73.968 kb on - strand at 73.994 kb on + strand at 73.994 kb on + strand at 74.250 kb on + strand, within GFF5699 at 74.250 kb on + strand, within GFF5699 at 74.251 kb on - strand, within GFF5699 at 74.327 kb on + strand, within GFF5699 at 74.327 kb on + strand, within GFF5699 at 74.327 kb on + strand, within GFF5699 at 74.328 kb on - strand, within GFF5699 at 74.328 kb on - strand, within GFF5699 at 74.930 kb on + strand, within GFF5700 at 75.053 kb on + strand, within GFF5700 at 75.576 kb on + strand, within GFF5701 at 75.576 kb on + strand, within GFF5701
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 73,136 + GFF5698 0.59 -0.6 73,136 + GFF5698 0.59 +0.1 73,136 + GFF5698 0.59 +0.1 73,136 + GFF5698 0.59 +1.2 73,136 + GFF5698 0.59 -0.5 73,137 - GFF5698 0.59 +0.1 73,137 - GFF5698 0.59 -0.3 73,137 - GFF5698 0.59 -1.1 73,137 - GFF5698 0.59 -0.3 73,181 + GFF5698 0.61 -0.6 73,181 + GFF5698 0.61 +0.0 73,181 + GFF5698 0.61 +0.1 73,182 - GFF5698 0.61 +1.4 73,182 - GFF5698 0.61 +0.4 73,182 - GFF5698 0.61 +0.3 73,241 + GFF5698 0.64 +1.3 73,242 - GFF5698 0.64 -0.7 73,242 - GFF5698 0.64 +0.2 73,242 - GFF5698 0.64 -0.5 73,242 - GFF5698 0.64 -2.1 73,253 + GFF5698 0.65 +0.2 73,254 - GFF5698 0.65 -0.4 73,254 - GFF5698 0.65 +0.5 73,254 - GFF5698 0.65 +0.3 73,277 + GFF5698 0.66 -0.6 73,326 - GFF5698 0.68 -1.8 73,343 + GFF5698 0.69 -0.6 73,343 + GFF5698 0.69 +0.6 73,394 + GFF5698 0.71 +0.7 73,394 + GFF5698 0.71 -2.2 73,395 - GFF5698 0.71 +0.8 73,395 - GFF5698 0.71 -0.1 73,395 - GFF5698 0.71 -0.0 73,395 - GFF5698 0.71 -1.8 73,415 + GFF5698 0.72 +0.9 73,415 + GFF5698 0.72 +1.5 73,535 + GFF5698 0.78 -2.4 73,536 - GFF5698 0.78 +0.6 73,571 + GFF5698 0.80 +1.2 73,571 + GFF5698 0.80 +0.3 73,571 + GFF5698 0.80 +0.0 73,571 + GFF5698 0.80 -0.4 73,622 + GFF5698 0.82 -1.9 73,622 + GFF5698 0.82 -1.2 73,622 + GFF5698 0.82 +0.2 73,623 - GFF5698 0.82 -0.8 73,652 + GFF5698 0.84 +0.2 73,676 + GFF5698 0.85 -1.1 73,677 - GFF5698 0.85 +1.6 73,734 - GFF5698 0.87 -1.5 73,734 - GFF5698 0.87 -0.9 73,773 - GFF5698 0.89 +0.1 73,773 - GFF5698 0.89 -0.2 73,773 - GFF5698 0.89 +0.4 73,967 + -0.1 73,968 - -0.5 73,968 - -0.4 73,994 + +0.1 73,994 + +0.3 74,250 + GFF5699 0.29 +0.1 74,250 + GFF5699 0.29 -0.9 74,251 - GFF5699 0.30 +2.1 74,327 + GFF5699 0.41 -0.1 74,327 + GFF5699 0.41 -0.4 74,327 + GFF5699 0.41 -0.4 74,328 - GFF5699 0.41 +0.1 74,328 - GFF5699 0.41 -0.9 74,930 + GFF5700 0.32 +0.2 75,053 + GFF5700 0.50 -0.4 75,576 + GFF5701 0.17 +0.6 75,576 + GFF5701 0.17 +1.3
Or see this region's nucleotide sequence