Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5667 and GFF5668 are separated by 111 nucleotides GFF5668 and GFF5669 overlap by 4 nucleotides GFF5669 and GFF5670 overlap by 4 nucleotides GFF5670 and GFF5671 are separated by 10 nucleotides
GFF5667 - Transcriptional regulator, LysR family, at 42,889 to 43,779
GFF5667
GFF5668 - Uncharacterized MFS-type transporter, at 43,891 to 45,402
GFF5668
GFF5669 - hypothetical protein, at 45,399 to 45,677
GFF5669
GFF5670 - Protein SlyX, at 45,674 to 45,865
GFF5670
GFF5671 - FIG01964566: Predicted membrane protein, hemolysin III homolog, at 45,876 to 46,562
GFF5671
Position (kb)
43
44
45
46 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 42.891 kb on - strand at 43.076 kb on + strand, within GFF5667 at 43.076 kb on + strand, within GFF5667 at 43.076 kb on + strand, within GFF5667 at 43.076 kb on + strand, within GFF5667 at 43.076 kb on + strand, within GFF5667 at 43.076 kb on + strand, within GFF5667 at 43.076 kb on + strand, within GFF5667 at 43.076 kb on + strand, within GFF5667 at 43.076 kb on + strand, within GFF5667 at 43.076 kb on + strand, within GFF5667 at 43.076 kb on + strand, within GFF5667 at 43.076 kb on + strand, within GFF5667 at 43.076 kb on + strand, within GFF5667 at 43.076 kb on + strand, within GFF5667 at 43.076 kb on + strand, within GFF5667 at 43.076 kb on + strand, within GFF5667 at 43.077 kb on - strand, within GFF5667 at 43.077 kb on - strand, within GFF5667 at 43.077 kb on - strand, within GFF5667 at 43.077 kb on - strand, within GFF5667 at 43.077 kb on - strand, within GFF5667 at 43.077 kb on - strand, within GFF5667 at 43.077 kb on - strand, within GFF5667 at 43.077 kb on - strand, within GFF5667 at 43.077 kb on - strand, within GFF5667 at 43.077 kb on - strand, within GFF5667 at 43.077 kb on - strand, within GFF5667 at 43.077 kb on - strand, within GFF5667 at 43.208 kb on + strand, within GFF5667 at 43.208 kb on + strand, within GFF5667 at 43.208 kb on + strand, within GFF5667 at 43.208 kb on + strand, within GFF5667 at 43.209 kb on - strand, within GFF5667 at 43.271 kb on + strand, within GFF5667 at 43.272 kb on - strand, within GFF5667 at 43.272 kb on - strand, within GFF5667 at 43.272 kb on - strand, within GFF5667 at 43.366 kb on + strand, within GFF5667 at 43.366 kb on + strand, within GFF5667 at 43.366 kb on + strand, within GFF5667 at 43.366 kb on + strand, within GFF5667 at 43.366 kb on + strand, within GFF5667 at 43.366 kb on + strand, within GFF5667 at 43.366 kb on + strand, within GFF5667 at 43.366 kb on + strand, within GFF5667 at 43.366 kb on + strand, within GFF5667 at 43.366 kb on + strand, within GFF5667 at 43.366 kb on + strand, within GFF5667 at 43.366 kb on + strand, within GFF5667 at 43.366 kb on + strand, within GFF5667 at 43.366 kb on + strand, within GFF5667 at 43.366 kb on + strand, within GFF5667 at 43.366 kb on + strand, within GFF5667 at 43.366 kb on + strand, within GFF5667 at 43.366 kb on + strand, within GFF5667 at 43.366 kb on + strand, within GFF5667 at 43.367 kb on - strand, within GFF5667 at 43.367 kb on - strand, within GFF5667 at 43.367 kb on - strand, within GFF5667 at 43.367 kb on - strand, within GFF5667 at 43.367 kb on - strand, within GFF5667 at 43.367 kb on - strand, within GFF5667 at 43.367 kb on - strand, within GFF5667 at 43.367 kb on - strand, within GFF5667 at 43.367 kb on - strand, within GFF5667 at 43.367 kb on - strand, within GFF5667 at 43.367 kb on - strand, within GFF5667 at 43.367 kb on - strand, within GFF5667 at 43.367 kb on - strand, within GFF5667 at 43.367 kb on - strand, within GFF5667 at 43.367 kb on - strand, within GFF5667 at 43.430 kb on + strand, within GFF5667 at 43.828 kb on + strand at 43.847 kb on + strand at 43.847 kb on + strand at 43.847 kb on + strand at 43.848 kb on - strand at 43.894 kb on + strand at 43.894 kb on + strand at 43.894 kb on + strand at 43.894 kb on + strand at 43.894 kb on + strand at 43.894 kb on + strand at 43.894 kb on + strand at 43.894 kb on + strand at 43.895 kb on - strand at 43.895 kb on - strand at 44.104 kb on + strand, within GFF5668 at 44.104 kb on + strand, within GFF5668 at 44.104 kb on + strand, within GFF5668 at 44.104 kb on + strand, within GFF5668 at 44.104 kb on + strand, within GFF5668 at 44.105 kb on - strand, within GFF5668 at 44.105 kb on - strand, within GFF5668 at 44.105 kb on - strand, within GFF5668 at 44.105 kb on - strand, within GFF5668 at 44.105 kb on - strand, within GFF5668 at 44.716 kb on + strand, within GFF5668 at 44.716 kb on + strand, within GFF5668 at 44.716 kb on + strand, within GFF5668 at 44.716 kb on + strand, within GFF5668 at 44.717 kb on - strand, within GFF5668 at 44.717 kb on - strand, within GFF5668 at 44.717 kb on - strand, within GFF5668 at 44.717 kb on - strand, within GFF5668 at 44.717 kb on - strand, within GFF5668 at 45.265 kb on + strand at 45.265 kb on + strand at 45.265 kb on + strand at 45.265 kb on + strand at 45.266 kb on - strand at 45.266 kb on - strand at 45.266 kb on - strand at 45.266 kb on - strand at 45.266 kb on - strand at 45.266 kb on - strand at 45.354 kb on + strand at 45.355 kb on - strand at 45.355 kb on - strand at 45.590 kb on - strand, within GFF5669 at 45.701 kb on + strand, within GFF5670 at 45.702 kb on - strand, within GFF5670 at 45.740 kb on + strand, within GFF5670 at 45.740 kb on + strand, within GFF5670 at 45.740 kb on + strand, within GFF5670 at 45.740 kb on + strand, within GFF5670 at 45.740 kb on + strand, within GFF5670 at 45.741 kb on - strand, within GFF5670 at 45.741 kb on - strand, within GFF5670 at 45.741 kb on - strand, within GFF5670 at 45.741 kb on - strand, within GFF5670 at 45.741 kb on - strand, within GFF5670 at 45.849 kb on + strand at 45.849 kb on + strand at 45.849 kb on + strand at 45.850 kb on - strand at 45.850 kb on - strand at 45.860 kb on + strand at 45.860 kb on + strand at 45.860 kb on + strand at 45.861 kb on - strand at 45.861 kb on - strand at 45.861 kb on - strand at 45.861 kb on - strand at 45.959 kb on - strand, within GFF5671 at 45.959 kb on - strand, within GFF5671 at 45.959 kb on - strand, within GFF5671 at 45.959 kb on - strand, within GFF5671 at 45.959 kb on - strand, within GFF5671 at 45.959 kb on - strand, within GFF5671 at 45.984 kb on + strand, within GFF5671 at 45.984 kb on + strand, within GFF5671 at 45.984 kb on + strand, within GFF5671 at 45.984 kb on + strand, within GFF5671 at 45.984 kb on + strand, within GFF5671 at 45.985 kb on - strand, within GFF5671 at 45.985 kb on - strand, within GFF5671 at 45.985 kb on - strand, within GFF5671 at 45.985 kb on - strand, within GFF5671 at 46.001 kb on + strand, within GFF5671 at 46.003 kb on + strand, within GFF5671 at 46.003 kb on + strand, within GFF5671 at 46.003 kb on + strand, within GFF5671 at 46.003 kb on + strand, within GFF5671 at 46.003 kb on + strand, within GFF5671 at 46.003 kb on + strand, within GFF5671 at 46.003 kb on + strand, within GFF5671 at 46.003 kb on + strand, within GFF5671 at 46.003 kb on + strand, within GFF5671 at 46.003 kb on + strand, within GFF5671 at 46.003 kb on + strand, within GFF5671 at 46.003 kb on + strand, within GFF5671 at 46.004 kb on - strand, within GFF5671 at 46.004 kb on - strand, within GFF5671 at 46.004 kb on - strand, within GFF5671 at 46.004 kb on - strand, within GFF5671 at 46.004 kb on - strand, within GFF5671 at 46.004 kb on - strand, within GFF5671 at 46.004 kb on - strand, within GFF5671 at 46.004 kb on - strand, within GFF5671 at 46.004 kb on - strand, within GFF5671 at 46.057 kb on + strand, within GFF5671 at 46.057 kb on + strand, within GFF5671 at 46.057 kb on + strand, within GFF5671 at 46.057 kb on + strand, within GFF5671 at 46.058 kb on - strand, within GFF5671 at 46.058 kb on - strand, within GFF5671 at 46.102 kb on + strand, within GFF5671 at 46.102 kb on + strand, within GFF5671 at 46.102 kb on + strand, within GFF5671 at 46.102 kb on + strand, within GFF5671 at 46.102 kb on + strand, within GFF5671 at 46.102 kb on + strand, within GFF5671 at 46.102 kb on + strand, within GFF5671 at 46.103 kb on - strand, within GFF5671 at 46.103 kb on - strand, within GFF5671 at 46.103 kb on - strand, within GFF5671 at 46.103 kb on - strand, within GFF5671 at 46.103 kb on - strand, within GFF5671 at 46.103 kb on - strand, within GFF5671 at 46.103 kb on - strand, within GFF5671 at 46.103 kb on - strand, within GFF5671 at 46.103 kb on - strand, within GFF5671 at 46.240 kb on + strand, within GFF5671 at 46.240 kb on + strand, within GFF5671 at 46.240 kb on + strand, within GFF5671 at 46.240 kb on + strand, within GFF5671 at 46.240 kb on + strand, within GFF5671 at 46.240 kb on + strand, within GFF5671 at 46.240 kb on + strand, within GFF5671 at 46.240 kb on + strand, within GFF5671 at 46.240 kb on + strand, within GFF5671 at 46.240 kb on + strand, within GFF5671 at 46.240 kb on + strand, within GFF5671 at 46.241 kb on - strand, within GFF5671 at 46.241 kb on - strand, within GFF5671 at 46.241 kb on - strand, within GFF5671 at 46.241 kb on - strand, within GFF5671 at 46.241 kb on - strand, within GFF5671 at 46.241 kb on - strand, within GFF5671 at 46.241 kb on - strand, within GFF5671 at 46.241 kb on - strand, within GFF5671 at 46.241 kb on - strand, within GFF5671 at 46.241 kb on - strand, within GFF5671 at 46.261 kb on + strand, within GFF5671 at 46.261 kb on + strand, within GFF5671 at 46.261 kb on + strand, within GFF5671 at 46.261 kb on + strand, within GFF5671 at 46.261 kb on + strand, within GFF5671 at 46.262 kb on - strand, within GFF5671 at 46.262 kb on - strand, within GFF5671 at 46.262 kb on - strand, within GFF5671 at 46.262 kb on - strand, within GFF5671 at 46.262 kb on - strand, within GFF5671 at 46.262 kb on - strand, within GFF5671 at 46.262 kb on - strand, within GFF5671 at 46.262 kb on - strand, within GFF5671 at 46.262 kb on - strand, within GFF5671 at 46.324 kb on + strand, within GFF5671 at 46.324 kb on + strand, within GFF5671 at 46.324 kb on + strand, within GFF5671 at 46.325 kb on - strand, within GFF5671 at 46.325 kb on - strand, within GFF5671 at 46.325 kb on - strand, within GFF5671 at 46.325 kb on - strand, within GFF5671 at 46.342 kb on + strand, within GFF5671 at 46.342 kb on + strand, within GFF5671 at 46.342 kb on + strand, within GFF5671 at 46.342 kb on + strand, within GFF5671 at 46.342 kb on + strand, within GFF5671 at 46.343 kb on - strand, within GFF5671 at 46.343 kb on - strand, within GFF5671 at 46.343 kb on - strand, within GFF5671 at 46.343 kb on - strand, within GFF5671
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 42,891 - +1.2 43,076 + GFF5667 0.21 +0.9 43,076 + GFF5667 0.21 +0.2 43,076 + GFF5667 0.21 +0.7 43,076 + GFF5667 0.21 -0.3 43,076 + GFF5667 0.21 +0.2 43,076 + GFF5667 0.21 +0.9 43,076 + GFF5667 0.21 -1.5 43,076 + GFF5667 0.21 +0.3 43,076 + GFF5667 0.21 -0.2 43,076 + GFF5667 0.21 -0.1 43,076 + GFF5667 0.21 -0.2 43,076 + GFF5667 0.21 -1.8 43,076 + GFF5667 0.21 +0.2 43,076 + GFF5667 0.21 +0.1 43,076 + GFF5667 0.21 -0.6 43,076 + GFF5667 0.21 +0.1 43,077 - GFF5667 0.21 +0.5 43,077 - GFF5667 0.21 -0.5 43,077 - GFF5667 0.21 -0.4 43,077 - GFF5667 0.21 +0.2 43,077 - GFF5667 0.21 +0.2 43,077 - GFF5667 0.21 +1.1 43,077 - GFF5667 0.21 -0.9 43,077 - GFF5667 0.21 +0.5 43,077 - GFF5667 0.21 -1.3 43,077 - GFF5667 0.21 +0.1 43,077 - GFF5667 0.21 -0.4 43,077 - GFF5667 0.21 -0.0 43,208 + GFF5667 0.36 +0.1 43,208 + GFF5667 0.36 -1.4 43,208 + GFF5667 0.36 +0.1 43,208 + GFF5667 0.36 -0.9 43,209 - GFF5667 0.36 +0.3 43,271 + GFF5667 0.43 -0.3 43,272 - GFF5667 0.43 +1.0 43,272 - GFF5667 0.43 +0.3 43,272 - GFF5667 0.43 +1.0 43,366 + GFF5667 0.54 -0.5 43,366 + GFF5667 0.54 +0.4 43,366 + GFF5667 0.54 +0.5 43,366 + GFF5667 0.54 +0.8 43,366 + GFF5667 0.54 +0.5 43,366 + GFF5667 0.54 -0.4 43,366 + GFF5667 0.54 -1.8 43,366 + GFF5667 0.54 -0.3 43,366 + GFF5667 0.54 -0.5 43,366 + GFF5667 0.54 -0.9 43,366 + GFF5667 0.54 -0.1 43,366 + GFF5667 0.54 -0.2 43,366 + GFF5667 0.54 -0.6 43,366 + GFF5667 0.54 +0.5 43,366 + GFF5667 0.54 +0.3 43,366 + GFF5667 0.54 +0.1 43,366 + GFF5667 0.54 +1.0 43,366 + GFF5667 0.54 -0.3 43,366 + GFF5667 0.54 -0.0 43,367 - GFF5667 0.54 -2.1 43,367 - GFF5667 0.54 -0.1 43,367 - GFF5667 0.54 +0.1 43,367 - GFF5667 0.54 -1.8 43,367 - GFF5667 0.54 +0.9 43,367 - GFF5667 0.54 +0.6 43,367 - GFF5667 0.54 -0.0 43,367 - GFF5667 0.54 +0.1 43,367 - GFF5667 0.54 +0.2 43,367 - GFF5667 0.54 -0.1 43,367 - GFF5667 0.54 -0.2 43,367 - GFF5667 0.54 +0.1 43,367 - GFF5667 0.54 +0.1 43,367 - GFF5667 0.54 -0.2 43,367 - GFF5667 0.54 +0.8 43,430 + GFF5667 0.61 -0.1 43,828 + -0.5 43,847 + +0.1 43,847 + -0.3 43,847 + +0.0 43,848 - -0.4 43,894 + +0.4 43,894 + +1.1 43,894 + -0.4 43,894 + -1.0 43,894 + -1.7 43,894 + -0.5 43,894 + -0.7 43,894 + -0.7 43,895 - +0.5 43,895 - -0.8 44,104 + GFF5668 0.14 +0.1 44,104 + GFF5668 0.14 -0.5 44,104 + GFF5668 0.14 -0.4 44,104 + GFF5668 0.14 -0.1 44,104 + GFF5668 0.14 -1.2 44,105 - GFF5668 0.14 -1.4 44,105 - GFF5668 0.14 -0.1 44,105 - GFF5668 0.14 -1.2 44,105 - GFF5668 0.14 +0.5 44,105 - GFF5668 0.14 -1.9 44,716 + GFF5668 0.55 -1.2 44,716 + GFF5668 0.55 +1.1 44,716 + GFF5668 0.55 -0.9 44,716 + GFF5668 0.55 +0.8 44,717 - GFF5668 0.55 -0.1 44,717 - GFF5668 0.55 +0.1 44,717 - GFF5668 0.55 -0.4 44,717 - GFF5668 0.55 -0.2 44,717 - GFF5668 0.55 -0.6 45,265 + -0.5 45,265 + +0.1 45,265 + +1.0 45,265 + -1.8 45,266 - -0.1 45,266 - -0.2 45,266 - +0.5 45,266 - -0.2 45,266 - +1.0 45,266 - -0.6 45,354 + +1.3 45,355 - -0.0 45,355 - -1.5 45,590 - GFF5669 0.68 +0.2 45,701 + GFF5670 0.14 -0.0 45,702 - GFF5670 0.15 -1.7 45,740 + GFF5670 0.34 +0.3 45,740 + GFF5670 0.34 -0.2 45,740 + GFF5670 0.34 -0.2 45,740 + GFF5670 0.34 -0.6 45,740 + GFF5670 0.34 -1.3 45,741 - GFF5670 0.35 -0.2 45,741 - GFF5670 0.35 -0.9 45,741 - GFF5670 0.35 -0.3 45,741 - GFF5670 0.35 -1.1 45,741 - GFF5670 0.35 -0.9 45,849 + -0.2 45,849 + -0.7 45,849 + -0.7 45,850 - -0.0 45,850 - -0.7 45,860 + +0.8 45,860 + +1.2 45,860 + -0.4 45,861 - +0.1 45,861 - +0.1 45,861 - -0.2 45,861 - -0.1 45,959 - GFF5671 0.12 +0.4 45,959 - GFF5671 0.12 +1.6 45,959 - GFF5671 0.12 -0.4 45,959 - GFF5671 0.12 -0.9 45,959 - GFF5671 0.12 -1.0 45,959 - GFF5671 0.12 -0.7 45,984 + GFF5671 0.16 -0.4 45,984 + GFF5671 0.16 +0.2 45,984 + GFF5671 0.16 +0.1 45,984 + GFF5671 0.16 +1.9 45,984 + GFF5671 0.16 +0.6 45,985 - GFF5671 0.16 -0.1 45,985 - GFF5671 0.16 -1.1 45,985 - GFF5671 0.16 +1.9 45,985 - GFF5671 0.16 -1.1 46,001 + GFF5671 0.18 -0.3 46,003 + GFF5671 0.18 -0.3 46,003 + GFF5671 0.18 +1.0 46,003 + GFF5671 0.18 -0.1 46,003 + GFF5671 0.18 +0.4 46,003 + GFF5671 0.18 +0.2 46,003 + GFF5671 0.18 -0.1 46,003 + GFF5671 0.18 -0.2 46,003 + GFF5671 0.18 +0.7 46,003 + GFF5671 0.18 +0.6 46,003 + GFF5671 0.18 -1.9 46,003 + GFF5671 0.18 -0.9 46,003 + GFF5671 0.18 -0.2 46,004 - GFF5671 0.19 -0.2 46,004 - GFF5671 0.19 -1.4 46,004 - GFF5671 0.19 +0.5 46,004 - GFF5671 0.19 -0.3 46,004 - GFF5671 0.19 +1.5 46,004 - GFF5671 0.19 +0.2 46,004 - GFF5671 0.19 -0.5 46,004 - GFF5671 0.19 -0.1 46,004 - GFF5671 0.19 -0.1 46,057 + GFF5671 0.26 +0.2 46,057 + GFF5671 0.26 -0.8 46,057 + GFF5671 0.26 +1.6 46,057 + GFF5671 0.26 +0.1 46,058 - GFF5671 0.26 -0.8 46,058 - GFF5671 0.26 -1.4 46,102 + GFF5671 0.33 -0.3 46,102 + GFF5671 0.33 -0.3 46,102 + GFF5671 0.33 +0.4 46,102 + GFF5671 0.33 -0.5 46,102 + GFF5671 0.33 -0.9 46,102 + GFF5671 0.33 -0.4 46,102 + GFF5671 0.33 -0.9 46,103 - GFF5671 0.33 -0.5 46,103 - GFF5671 0.33 +0.0 46,103 - GFF5671 0.33 -0.5 46,103 - GFF5671 0.33 -0.5 46,103 - GFF5671 0.33 -0.6 46,103 - GFF5671 0.33 -0.1 46,103 - GFF5671 0.33 -0.6 46,103 - GFF5671 0.33 -0.3 46,103 - GFF5671 0.33 +0.9 46,240 + GFF5671 0.53 -0.1 46,240 + GFF5671 0.53 -0.3 46,240 + GFF5671 0.53 -0.2 46,240 + GFF5671 0.53 -0.2 46,240 + GFF5671 0.53 +0.2 46,240 + GFF5671 0.53 +0.9 46,240 + GFF5671 0.53 -0.9 46,240 + GFF5671 0.53 -0.2 46,240 + GFF5671 0.53 +2.4 46,240 + GFF5671 0.53 -0.6 46,240 + GFF5671 0.53 -0.5 46,241 - GFF5671 0.53 +0.3 46,241 - GFF5671 0.53 -1.0 46,241 - GFF5671 0.53 +0.2 46,241 - GFF5671 0.53 -0.7 46,241 - GFF5671 0.53 -0.5 46,241 - GFF5671 0.53 +0.4 46,241 - GFF5671 0.53 -0.8 46,241 - GFF5671 0.53 -0.7 46,241 - GFF5671 0.53 +1.6 46,241 - GFF5671 0.53 -0.3 46,261 + GFF5671 0.56 -0.3 46,261 + GFF5671 0.56 +0.4 46,261 + GFF5671 0.56 +1.1 46,261 + GFF5671 0.56 +0.7 46,261 + GFF5671 0.56 -0.0 46,262 - GFF5671 0.56 -1.2 46,262 - GFF5671 0.56 +0.2 46,262 - GFF5671 0.56 -0.9 46,262 - GFF5671 0.56 +0.7 46,262 - GFF5671 0.56 -0.7 46,262 - GFF5671 0.56 -1.4 46,262 - GFF5671 0.56 -0.2 46,262 - GFF5671 0.56 +0.2 46,262 - GFF5671 0.56 +0.0 46,324 + GFF5671 0.65 +0.4 46,324 + GFF5671 0.65 -0.3 46,324 + GFF5671 0.65 +0.9 46,325 - GFF5671 0.65 -0.4 46,325 - GFF5671 0.65 -0.4 46,325 - GFF5671 0.65 +1.2 46,325 - GFF5671 0.65 -1.4 46,342 + GFF5671 0.68 -1.2 46,342 + GFF5671 0.68 -0.4 46,342 + GFF5671 0.68 +0.3 46,342 + GFF5671 0.68 -0.5 46,342 + GFF5671 0.68 -0.3 46,343 - GFF5671 0.68 -0.4 46,343 - GFF5671 0.68 +0.2 46,343 - GFF5671 0.68 -1.2 46,343 - GFF5671 0.68 -0.1
Or see this region's nucleotide sequence