Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5616 and GFF5617 overlap by 10 nucleotides GFF5617 and GFF5618 are separated by 118 nucleotides
GFF5616 - hypothetical protein, at 238,850 to 239,887
GFF5616
GFF5617 - Coenzyme A ligase, at 239,878 to 241,128
GFF5617
GFF5618 - ABC transporter, ATP-binding protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 241,247 to 242,059
GFF5618
Position (kb) 239
240
241
242 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 239.012 kb on + strand, within GFF5616 at 239.013 kb on - strand, within GFF5616 at 239.013 kb on - strand, within GFF5616 at 239.174 kb on + strand, within GFF5616 at 239.201 kb on + strand, within GFF5616 at 239.201 kb on + strand, within GFF5616 at 239.202 kb on - strand, within GFF5616 at 239.202 kb on - strand, within GFF5616 at 239.223 kb on - strand, within GFF5616 at 239.744 kb on + strand, within GFF5616 at 239.744 kb on + strand, within GFF5616 at 239.744 kb on + strand, within GFF5616 at 239.744 kb on + strand, within GFF5616 at 239.745 kb on - strand, within GFF5616 at 239.745 kb on - strand, within GFF5616 at 239.870 kb on + strand at 239.871 kb on - strand at 239.879 kb on + strand at 239.885 kb on + strand at 240.404 kb on + strand, within GFF5617 at 240.404 kb on + strand, within GFF5617 at 240.434 kb on + strand, within GFF5617 at 240.435 kb on - strand, within GFF5617 at 240.435 kb on - strand, within GFF5617 at 240.565 kb on + strand, within GFF5617 at 240.565 kb on + strand, within GFF5617 at 240.565 kb on + strand, within GFF5617 at 240.565 kb on + strand, within GFF5617 at 240.566 kb on - strand, within GFF5617 at 240.723 kb on - strand, within GFF5617 at 240.821 kb on + strand, within GFF5617 at 240.822 kb on - strand, within GFF5617 at 241.276 kb on - strand at 241.276 kb on - strand at 241.276 kb on - strand at 241.276 kb on - strand at 241.405 kb on - strand, within GFF5618 at 241.405 kb on - strand, within GFF5618 at 241.410 kb on + strand, within GFF5618 at 241.410 kb on + strand, within GFF5618 at 241.411 kb on - strand, within GFF5618 at 241.620 kb on + strand, within GFF5618 at 241.621 kb on - strand, within GFF5618 at 241.668 kb on + strand, within GFF5618 at 241.668 kb on + strand, within GFF5618 at 241.668 kb on + strand, within GFF5618 at 241.668 kb on + strand, within GFF5618 at 241.668 kb on + strand, within GFF5618 at 241.669 kb on - strand, within GFF5618 at 241.669 kb on - strand, within GFF5618 at 241.669 kb on - strand, within GFF5618 at 241.669 kb on - strand, within GFF5618 at 241.669 kb on - strand, within GFF5618 at 242.010 kb on + strand at 242.010 kb on + strand at 242.010 kb on + strand at 242.010 kb on + strand at 242.010 kb on + strand at 242.011 kb on - strand at 242.011 kb on - strand at 242.011 kb on - strand at 242.011 kb on - strand at 242.011 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 239,012 + GFF5616 0.16 -0.2 239,013 - GFF5616 0.16 -0.7 239,013 - GFF5616 0.16 +0.3 239,174 + GFF5616 0.31 -2.0 239,201 + GFF5616 0.34 -0.2 239,201 + GFF5616 0.34 -0.2 239,202 - GFF5616 0.34 -0.4 239,202 - GFF5616 0.34 +0.3 239,223 - GFF5616 0.36 +0.8 239,744 + GFF5616 0.86 -0.4 239,744 + GFF5616 0.86 -0.1 239,744 + GFF5616 0.86 +0.2 239,744 + GFF5616 0.86 +0.2 239,745 - GFF5616 0.86 -0.9 239,745 - GFF5616 0.86 +0.1 239,870 + -1.6 239,871 - +1.4 239,879 + -0.1 239,885 + -3.2 240,404 + GFF5617 0.42 +1.4 240,404 + GFF5617 0.42 -0.0 240,434 + GFF5617 0.44 -0.4 240,435 - GFF5617 0.45 +1.8 240,435 - GFF5617 0.45 -1.8 240,565 + GFF5617 0.55 -0.4 240,565 + GFF5617 0.55 -0.4 240,565 + GFF5617 0.55 -0.8 240,565 + GFF5617 0.55 -1.1 240,566 - GFF5617 0.55 -0.8 240,723 - GFF5617 0.68 +0.5 240,821 + GFF5617 0.75 +0.6 240,822 - GFF5617 0.75 -1.0 241,276 - -0.3 241,276 - +0.5 241,276 - -0.4 241,276 - -0.1 241,405 - GFF5618 0.19 -0.3 241,405 - GFF5618 0.19 +0.5 241,410 + GFF5618 0.20 -0.0 241,410 + GFF5618 0.20 -1.4 241,411 - GFF5618 0.20 -0.5 241,620 + GFF5618 0.46 -0.1 241,621 - GFF5618 0.46 -0.5 241,668 + GFF5618 0.52 -0.6 241,668 + GFF5618 0.52 -0.3 241,668 + GFF5618 0.52 -0.1 241,668 + GFF5618 0.52 -0.3 241,668 + GFF5618 0.52 -0.1 241,669 - GFF5618 0.52 +0.2 241,669 - GFF5618 0.52 -1.2 241,669 - GFF5618 0.52 -0.3 241,669 - GFF5618 0.52 +0.2 241,669 - GFF5618 0.52 -0.3 242,010 + +1.8 242,010 + -0.1 242,010 + -0.7 242,010 + -0.2 242,010 + -0.9 242,011 - +3.1 242,011 - +0.2 242,011 - -0.1 242,011 - +1.1 242,011 - +0.3
Or see this region's nucleotide sequence