Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5573 and GFF5574 are separated by 30 nucleotides GFF5574 and GFF5575 overlap by 4 nucleotides GFF5575 and GFF5576 are separated by 5 nucleotides
GFF5573 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 193,399 to 194,373
GFF5573
GFF5574 - RidA/YER057c/UK114 superfamily protein, at 194,404 to 194,805
GFF5574
GFF5575 - Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases, at 194,802 to 196,424
GFF5575
GFF5576 - Acyl-CoA dehydrogenase, at 196,430 to 197,623
GFF5576
Position (kb) 194
195
196
197 Strain fitness (log2 ratio) -1
0
1
2 at 193.822 kb on + strand, within GFF5573 at 193.822 kb on + strand, within GFF5573 at 193.823 kb on - strand, within GFF5573 at 194.896 kb on + strand at 195.098 kb on - strand, within GFF5575 at 195.098 kb on - strand, within GFF5575 at 195.137 kb on - strand, within GFF5575 at 195.181 kb on + strand, within GFF5575 at 195.202 kb on + strand, within GFF5575 at 195.202 kb on + strand, within GFF5575 at 195.203 kb on - strand, within GFF5575 at 195.203 kb on - strand, within GFF5575 at 195.355 kb on - strand, within GFF5575 at 195.355 kb on - strand, within GFF5575 at 195.378 kb on - strand, within GFF5575 at 195.378 kb on - strand, within GFF5575 at 195.378 kb on - strand, within GFF5575 at 195.379 kb on + strand, within GFF5575 at 195.380 kb on - strand, within GFF5575 at 195.380 kb on - strand, within GFF5575 at 195.380 kb on - strand, within GFF5575 at 195.541 kb on + strand, within GFF5575 at 195.542 kb on - strand, within GFF5575 at 195.542 kb on - strand, within GFF5575 at 195.559 kb on + strand, within GFF5575 at 195.559 kb on + strand, within GFF5575 at 195.559 kb on + strand, within GFF5575 at 195.560 kb on - strand, within GFF5575 at 195.631 kb on + strand, within GFF5575 at 195.631 kb on + strand, within GFF5575 at 195.631 kb on + strand, within GFF5575 at 195.632 kb on - strand, within GFF5575 at 195.632 kb on - strand, within GFF5575 at 196.231 kb on + strand, within GFF5575 at 196.231 kb on + strand, within GFF5575 at 196.232 kb on - strand, within GFF5575 at 196.427 kb on + strand at 196.427 kb on + strand at 196.427 kb on + strand at 196.428 kb on - strand at 196.428 kb on - strand at 196.428 kb on - strand at 196.428 kb on - strand at 196.431 kb on + strand at 196.432 kb on - strand at 196.432 kb on - strand at 196.488 kb on + strand at 196.488 kb on + strand at 196.488 kb on + strand at 196.489 kb on - strand at 196.516 kb on - strand at 196.698 kb on + strand, within GFF5576 at 196.698 kb on + strand, within GFF5576 at 196.702 kb on + strand, within GFF5576 at 196.702 kb on + strand, within GFF5576 at 196.702 kb on + strand, within GFF5576 at 196.702 kb on + strand, within GFF5576 at 196.703 kb on - strand, within GFF5576 at 196.703 kb on - strand, within GFF5576 at 196.703 kb on - strand, within GFF5576 at 196.703 kb on - strand, within GFF5576 at 196.703 kb on - strand, within GFF5576 at 196.703 kb on - strand, within GFF5576 at 197.094 kb on + strand, within GFF5576 at 197.095 kb on - strand, within GFF5576 at 197.095 kb on - strand, within GFF5576 at 197.095 kb on - strand, within GFF5576 at 197.095 kb on - strand, within GFF5576 at 197.263 kb on - strand, within GFF5576 at 197.263 kb on - strand, within GFF5576 at 197.299 kb on + strand, within GFF5576 at 197.355 kb on + strand, within GFF5576 at 197.356 kb on - strand, within GFF5576
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 193,822 + GFF5573 0.43 -0.4 193,822 + GFF5573 0.43 -1.0 193,823 - GFF5573 0.43 -0.4 194,896 + +0.5 195,098 - GFF5575 0.18 -0.6 195,098 - GFF5575 0.18 +0.8 195,137 - GFF5575 0.21 +0.6 195,181 + GFF5575 0.23 -0.0 195,202 + GFF5575 0.25 -0.2 195,202 + GFF5575 0.25 +0.3 195,203 - GFF5575 0.25 +0.0 195,203 - GFF5575 0.25 -0.1 195,355 - GFF5575 0.34 +0.2 195,355 - GFF5575 0.34 +0.0 195,378 - GFF5575 0.35 +0.4 195,378 - GFF5575 0.35 +0.7 195,378 - GFF5575 0.35 +0.4 195,379 + GFF5575 0.36 +0.4 195,380 - GFF5575 0.36 +0.2 195,380 - GFF5575 0.36 +0.7 195,380 - GFF5575 0.36 +1.1 195,541 + GFF5575 0.46 +0.7 195,542 - GFF5575 0.46 -0.4 195,542 - GFF5575 0.46 -1.5 195,559 + GFF5575 0.47 -1.2 195,559 + GFF5575 0.47 +0.8 195,559 + GFF5575 0.47 -0.0 195,560 - GFF5575 0.47 -0.5 195,631 + GFF5575 0.51 +2.0 195,631 + GFF5575 0.51 -0.1 195,631 + GFF5575 0.51 -0.6 195,632 - GFF5575 0.51 -0.1 195,632 - GFF5575 0.51 -0.1 196,231 + GFF5575 0.88 +0.0 196,231 + GFF5575 0.88 -0.3 196,232 - GFF5575 0.88 -0.0 196,427 + +0.2 196,427 + -0.6 196,427 + -0.3 196,428 - -0.5 196,428 - +1.6 196,428 - -0.7 196,428 - +1.3 196,431 + -0.0 196,432 - -0.1 196,432 - -0.2 196,488 + -0.8 196,488 + -0.8 196,488 + +0.0 196,489 - -0.4 196,516 - -0.6 196,698 + GFF5576 0.22 +0.6 196,698 + GFF5576 0.22 -0.2 196,702 + GFF5576 0.23 +0.3 196,702 + GFF5576 0.23 -1.3 196,702 + GFF5576 0.23 +0.8 196,702 + GFF5576 0.23 -0.4 196,703 - GFF5576 0.23 +0.2 196,703 - GFF5576 0.23 +0.6 196,703 - GFF5576 0.23 -0.5 196,703 - GFF5576 0.23 +1.0 196,703 - GFF5576 0.23 -0.1 196,703 - GFF5576 0.23 +0.1 197,094 + GFF5576 0.56 -0.2 197,095 - GFF5576 0.56 +0.8 197,095 - GFF5576 0.56 -0.5 197,095 - GFF5576 0.56 -0.1 197,095 - GFF5576 0.56 +0.2 197,263 - GFF5576 0.70 -0.9 197,263 - GFF5576 0.70 +0.1 197,299 + GFF5576 0.73 +1.2 197,355 + GFF5576 0.77 +1.2 197,356 - GFF5576 0.78 +0.7
Or see this region's nucleotide sequence