Strain Fitness in Variovorax sp. SCN45 around GFF5540

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5538 and GFF5539 are separated by 6 nucleotidesGFF5539 and GFF5540 are separated by 114 nucleotidesGFF5540 and GFF5541 are separated by 15 nucleotidesGFF5541 and GFF5542 overlap by 4 nucleotidesGFF5542 and GFF5543 are separated by 173 nucleotides GFF5538 - ATP-dependent DNA helicase RecG (EC 3.6.4.12), at 167,063 to 169,195 GFF5538 GFF5539 - Transporter, LysE family, at 169,202 to 169,834 GFF5539 GFF5540 - FIG01201438: hypothetical protein, at 169,949 to 170,389 GFF5540 GFF5541 - Transcriptional regulator, GntR family, at 170,405 to 171,109 GFF5541 GFF5542 - no description, at 171,106 to 171,207 GFF5542 GFF5543 - FIG015373: Membrane protein, at 171,381 to 172,118 GFF5543 Position (kb) 169 170 171Strain fitness (log2 ratio) -2 -1 0 1 2 3at 169.181 kb on + strandat 169.181 kb on + strandat 169.335 kb on + strand, within GFF5539at 169.335 kb on + strand, within GFF5539at 169.335 kb on + strand, within GFF5539at 169.335 kb on + strand, within GFF5539at 169.335 kb on + strand, within GFF5539at 169.335 kb on + strand, within GFF5539at 169.336 kb on - strand, within GFF5539at 169.336 kb on - strand, within GFF5539at 169.336 kb on - strand, within GFF5539at 169.336 kb on - strand, within GFF5539at 169.336 kb on - strand, within GFF5539at 169.389 kb on + strand, within GFF5539at 169.389 kb on + strand, within GFF5539at 169.389 kb on + strand, within GFF5539at 169.389 kb on + strand, within GFF5539at 169.389 kb on + strand, within GFF5539at 169.389 kb on + strand, within GFF5539at 169.389 kb on + strand, within GFF5539at 169.390 kb on - strand, within GFF5539at 169.390 kb on - strand, within GFF5539at 169.390 kb on - strand, within GFF5539at 169.390 kb on - strand, within GFF5539at 169.390 kb on - strand, within GFF5539at 169.401 kb on + strand, within GFF5539at 169.590 kb on + strand, within GFF5539at 169.590 kb on + strand, within GFF5539at 169.591 kb on - strand, within GFF5539at 169.591 kb on - strand, within GFF5539at 169.591 kb on - strand, within GFF5539at 169.605 kb on + strand, within GFF5539at 169.605 kb on + strand, within GFF5539at 169.605 kb on + strand, within GFF5539at 169.605 kb on + strand, within GFF5539at 169.605 kb on + strand, within GFF5539at 169.605 kb on + strand, within GFF5539at 169.605 kb on + strand, within GFF5539at 169.605 kb on + strand, within GFF5539at 169.605 kb on + strand, within GFF5539at 169.605 kb on + strand, within GFF5539at 169.605 kb on + strand, within GFF5539at 169.605 kb on + strand, within GFF5539at 169.606 kb on - strand, within GFF5539at 169.606 kb on - strand, within GFF5539at 169.606 kb on - strand, within GFF5539at 169.606 kb on - strand, within GFF5539at 169.606 kb on - strand, within GFF5539at 169.818 kb on + strandat 169.818 kb on + strandat 169.819 kb on - strandat 169.819 kb on - strandat 169.819 kb on - strandat 169.839 kb on + strandat 169.839 kb on + strandat 169.839 kb on + strandat 169.871 kb on + strandat 169.871 kb on + strandat 169.872 kb on - strandat 169.874 kb on - strandat 170.049 kb on - strand, within GFF5540at 170.049 kb on - strand, within GFF5540at 170.049 kb on - strand, within GFF5540at 170.049 kb on - strand, within GFF5540at 170.049 kb on - strand, within GFF5540at 170.333 kb on + strand, within GFF5540at 170.333 kb on + strand, within GFF5540at 170.333 kb on + strand, within GFF5540at 170.334 kb on - strand, within GFF5540at 170.334 kb on - strand, within GFF5540at 170.334 kb on - strand, within GFF5540at 170.334 kb on - strand, within GFF5540at 170.334 kb on - strand, within GFF5540at 170.334 kb on - strand, within GFF5540at 170.334 kb on - strand, within GFF5540at 170.442 kb on + strandat 170.442 kb on + strandat 170.443 kb on - strandat 170.443 kb on - strandat 170.443 kb on - strandat 170.443 kb on - strandat 170.443 kb on - strandat 170.443 kb on - strandat 170.478 kb on + strand, within GFF5541at 170.478 kb on + strand, within GFF5541at 170.478 kb on + strand, within GFF5541at 170.478 kb on + strand, within GFF5541at 170.479 kb on - strand, within GFF5541at 170.479 kb on - strand, within GFF5541at 170.479 kb on - strand, within GFF5541at 170.479 kb on - strand, within GFF5541at 170.479 kb on - strand, within GFF5541at 170.479 kb on - strand, within GFF5541at 170.556 kb on + strand, within GFF5541at 170.556 kb on + strand, within GFF5541at 170.557 kb on - strand, within GFF5541at 170.784 kb on + strand, within GFF5541at 170.784 kb on + strand, within GFF5541at 170.785 kb on - strand, within GFF5541at 170.829 kb on + strand, within GFF5541at 170.829 kb on + strand, within GFF5541at 170.829 kb on + strand, within GFF5541at 170.829 kb on + strand, within GFF5541at 170.829 kb on + strand, within GFF5541at 170.829 kb on + strand, within GFF5541at 170.830 kb on - strand, within GFF5541at 170.830 kb on - strand, within GFF5541at 170.830 kb on - strand, within GFF5541at 170.830 kb on - strand, within GFF5541at 170.830 kb on - strand, within GFF5541at 170.830 kb on - strand, within GFF5541at 170.830 kb on - strand, within GFF5541at 170.830 kb on - strand, within GFF5541at 170.830 kb on - strand, within GFF5541at 170.830 kb on - strand, within GFF5541at 170.830 kb on - strand, within GFF5541at 170.830 kb on - strand, within GFF5541at 170.830 kb on - strand, within GFF5541at 171.119 kb on + strand, within GFF5542at 171.119 kb on + strand, within GFF5542at 171.119 kb on + strand, within GFF5542at 171.119 kb on + strand, within GFF5542at 171.119 kb on + strand, within GFF5542at 171.119 kb on + strand, within GFF5542at 171.120 kb on - strand, within GFF5542at 171.120 kb on - strand, within GFF5542at 171.120 kb on - strand, within GFF5542at 171.120 kb on - strand, within GFF5542at 171.120 kb on - strand, within GFF5542at 171.120 kb on - strand, within GFF5542at 171.120 kb on - strand, within GFF5542at 171.120 kb on - strand, within GFF5542at 171.120 kb on - strand, within GFF5542at 171.152 kb on - strand, within GFF5542at 171.152 kb on - strand, within GFF5542at 171.152 kb on - strand, within GFF5542at 171.152 kb on - strand, within GFF5542at 171.152 kb on - strand, within GFF5542at 171.152 kb on - strand, within GFF5542at 171.152 kb on - strand, within GFF5542at 171.268 kb on + strandat 171.269 kb on - strandat 171.269 kb on - strandat 171.269 kb on - strandat 171.269 kb on - strandat 171.269 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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169,181 + -0.3
169,181 + -1.3
169,335 + GFF5539 0.21 -0.1
169,335 + GFF5539 0.21 +1.0
169,335 + GFF5539 0.21 -1.3
169,335 + GFF5539 0.21 +0.0
169,335 + GFF5539 0.21 -0.5
169,335 + GFF5539 0.21 -1.2
169,336 - GFF5539 0.21 +1.2
169,336 - GFF5539 0.21 +1.4
169,336 - GFF5539 0.21 -0.6
169,336 - GFF5539 0.21 +1.3
169,336 - GFF5539 0.21 +0.4
169,389 + GFF5539 0.30 +0.8
169,389 + GFF5539 0.30 +0.1
169,389 + GFF5539 0.30 -0.3
169,389 + GFF5539 0.30 +2.8
169,389 + GFF5539 0.30 -0.5
169,389 + GFF5539 0.30 -0.8
169,389 + GFF5539 0.30 +0.2
169,390 - GFF5539 0.30 -0.3
169,390 - GFF5539 0.30 -0.2
169,390 - GFF5539 0.30 +0.0
169,390 - GFF5539 0.30 +0.2
169,390 - GFF5539 0.30 +0.8
169,401 + GFF5539 0.31 -0.4
169,590 + GFF5539 0.61 +0.5
169,590 + GFF5539 0.61 +0.2
169,591 - GFF5539 0.61 +0.3
169,591 - GFF5539 0.61 -0.6
169,591 - GFF5539 0.61 +0.0
169,605 + GFF5539 0.64 -2.5
169,605 + GFF5539 0.64 +0.2
169,605 + GFF5539 0.64 +0.2
169,605 + GFF5539 0.64 -0.8
169,605 + GFF5539 0.64 -0.5
169,605 + GFF5539 0.64 +0.1
169,605 + GFF5539 0.64 -0.4
169,605 + GFF5539 0.64 -0.2
169,605 + GFF5539 0.64 +0.1
169,605 + GFF5539 0.64 +0.8
169,605 + GFF5539 0.64 -0.5
169,605 + GFF5539 0.64 +0.2
169,606 - GFF5539 0.64 -1.0
169,606 - GFF5539 0.64 +1.1
169,606 - GFF5539 0.64 +0.1
169,606 - GFF5539 0.64 -0.3
169,606 - GFF5539 0.64 +0.5
169,818 + +1.6
169,818 + -1.1
169,819 - +0.0
169,819 - +0.5
169,819 - -0.3
169,839 + +0.3
169,839 + -0.3
169,839 + +0.5
169,871 + -1.9
169,871 + -0.8
169,872 - +0.3
169,874 - +0.1
170,049 - GFF5540 0.23 -0.9
170,049 - GFF5540 0.23 -0.1
170,049 - GFF5540 0.23 -1.2
170,049 - GFF5540 0.23 +0.4
170,049 - GFF5540 0.23 -0.5
170,333 + GFF5540 0.87 -0.4
170,333 + GFF5540 0.87 -0.1
170,333 + GFF5540 0.87 -1.0
170,334 - GFF5540 0.87 -0.3
170,334 - GFF5540 0.87 -0.0
170,334 - GFF5540 0.87 +0.1
170,334 - GFF5540 0.87 -0.8
170,334 - GFF5540 0.87 -0.6
170,334 - GFF5540 0.87 -1.4
170,334 - GFF5540 0.87 -0.9
170,442 + +0.6
170,442 + +0.2
170,443 - -1.0
170,443 - -0.4
170,443 - +0.8
170,443 - +0.4
170,443 - +0.4
170,443 - +0.8
170,478 + GFF5541 0.10 +0.1
170,478 + GFF5541 0.10 +0.9
170,478 + GFF5541 0.10 +0.5
170,478 + GFF5541 0.10 -2.2
170,479 - GFF5541 0.10 -2.4
170,479 - GFF5541 0.10 +0.6
170,479 - GFF5541 0.10 -0.2
170,479 - GFF5541 0.10 -1.1
170,479 - GFF5541 0.10 +0.8
170,479 - GFF5541 0.10 +1.2
170,556 + GFF5541 0.21 -0.2
170,556 + GFF5541 0.21 -1.0
170,557 - GFF5541 0.22 +0.2
170,784 + GFF5541 0.54 +0.3
170,784 + GFF5541 0.54 +0.1
170,785 - GFF5541 0.54 -0.3
170,829 + GFF5541 0.60 -1.2
170,829 + GFF5541 0.60 +0.8
170,829 + GFF5541 0.60 -0.2
170,829 + GFF5541 0.60 -0.3
170,829 + GFF5541 0.60 -1.9
170,829 + GFF5541 0.60 -0.8
170,830 - GFF5541 0.60 +2.1
170,830 - GFF5541 0.60 -0.4
170,830 - GFF5541 0.60 -1.0
170,830 - GFF5541 0.60 -0.7
170,830 - GFF5541 0.60 -0.6
170,830 - GFF5541 0.60 +0.4
170,830 - GFF5541 0.60 -0.6
170,830 - GFF5541 0.60 -0.0
170,830 - GFF5541 0.60 +0.4
170,830 - GFF5541 0.60 -0.8
170,830 - GFF5541 0.60 -0.2
170,830 - GFF5541 0.60 -1.1
170,830 - GFF5541 0.60 +0.1
171,119 + GFF5542 0.13 +0.7
171,119 + GFF5542 0.13 -0.6
171,119 + GFF5542 0.13 -1.0
171,119 + GFF5542 0.13 -0.6
171,119 + GFF5542 0.13 +0.0
171,119 + GFF5542 0.13 -0.5
171,120 - GFF5542 0.14 -0.1
171,120 - GFF5542 0.14 -0.2
171,120 - GFF5542 0.14 +0.8
171,120 - GFF5542 0.14 -0.5
171,120 - GFF5542 0.14 +1.1
171,120 - GFF5542 0.14 +1.1
171,120 - GFF5542 0.14 -1.0
171,120 - GFF5542 0.14 -1.0
171,120 - GFF5542 0.14 +0.1
171,152 - GFF5542 0.45 -0.7
171,152 - GFF5542 0.45 +0.7
171,152 - GFF5542 0.45 +1.8
171,152 - GFF5542 0.45 -1.6
171,152 - GFF5542 0.45 -0.0
171,152 - GFF5542 0.45 -0.9
171,152 - GFF5542 0.45 -0.4
171,268 + -0.5
171,269 - +0.0
171,269 - +1.1
171,269 - +0.2
171,269 - +0.4
171,269 - -1.2

Or see this region's nucleotide sequence