Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5401 and GFF5402 are separated by 13 nucleotides GFF5402 and GFF5403 are separated by 64 nucleotides GFF5403 and GFF5404 are separated by 18 nucleotides
GFF5401 - 2-keto-3-deoxy-L-fuconate dehydrogenase, at 25,175 to 25,945
GFF5401
GFF5402 - Transcriptional regulator, IclR family, at 25,959 to 26,813
GFF5402
GFF5403 - no description, at 26,878 to 27,594
GFF5403
GFF5404 - N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38), at 27,613 to 28,536
GFF5404
Position (kb) 26
27
28 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 25.918 kb on + strand at 25.918 kb on + strand at 25.918 kb on + strand at 25.918 kb on + strand at 25.919 kb on - strand at 25.919 kb on - strand at 25.919 kb on - strand at 25.919 kb on - strand at 26.047 kb on - strand, within GFF5402 at 26.047 kb on - strand, within GFF5402 at 26.047 kb on - strand, within GFF5402 at 26.112 kb on + strand, within GFF5402 at 26.112 kb on + strand, within GFF5402 at 26.113 kb on - strand, within GFF5402 at 26.113 kb on - strand, within GFF5402 at 26.223 kb on + strand, within GFF5402 at 26.223 kb on + strand, within GFF5402 at 26.223 kb on + strand, within GFF5402 at 26.223 kb on + strand, within GFF5402 at 26.224 kb on - strand, within GFF5402 at 26.224 kb on - strand, within GFF5402 at 26.224 kb on - strand, within GFF5402 at 26.224 kb on - strand, within GFF5402 at 26.224 kb on - strand, within GFF5402 at 26.224 kb on - strand, within GFF5402 at 26.352 kb on + strand, within GFF5402 at 26.352 kb on + strand, within GFF5402 at 26.352 kb on + strand, within GFF5402 at 26.352 kb on + strand, within GFF5402 at 26.352 kb on + strand, within GFF5402 at 26.352 kb on + strand, within GFF5402 at 26.352 kb on + strand, within GFF5402 at 26.352 kb on + strand, within GFF5402 at 26.352 kb on + strand, within GFF5402 at 26.352 kb on + strand, within GFF5402 at 26.352 kb on + strand, within GFF5402 at 26.352 kb on + strand, within GFF5402 at 26.352 kb on + strand, within GFF5402 at 26.352 kb on + strand, within GFF5402 at 26.352 kb on + strand, within GFF5402 at 26.352 kb on + strand, within GFF5402 at 26.352 kb on + strand, within GFF5402 at 26.352 kb on + strand, within GFF5402 at 26.352 kb on + strand, within GFF5402 at 26.352 kb on + strand, within GFF5402 at 26.352 kb on + strand, within GFF5402 at 26.352 kb on + strand, within GFF5402 at 26.352 kb on + strand, within GFF5402 at 26.353 kb on - strand, within GFF5402 at 26.353 kb on - strand, within GFF5402 at 26.353 kb on - strand, within GFF5402 at 26.353 kb on - strand, within GFF5402 at 26.353 kb on - strand, within GFF5402 at 26.353 kb on - strand, within GFF5402 at 26.353 kb on - strand, within GFF5402 at 26.353 kb on - strand, within GFF5402 at 26.353 kb on - strand, within GFF5402 at 26.353 kb on - strand, within GFF5402 at 26.353 kb on - strand, within GFF5402 at 26.353 kb on - strand, within GFF5402 at 26.353 kb on - strand, within GFF5402 at 26.353 kb on - strand, within GFF5402 at 26.353 kb on - strand, within GFF5402 at 26.353 kb on - strand, within GFF5402 at 26.353 kb on - strand, within GFF5402 at 26.353 kb on - strand, within GFF5402 at 26.353 kb on - strand, within GFF5402 at 26.353 kb on - strand, within GFF5402 at 26.353 kb on - strand, within GFF5402 at 26.353 kb on - strand, within GFF5402 at 26.353 kb on - strand, within GFF5402 at 26.353 kb on - strand, within GFF5402 at 26.353 kb on - strand, within GFF5402 at 26.353 kb on - strand, within GFF5402 at 26.353 kb on - strand, within GFF5402 at 26.353 kb on - strand, within GFF5402 at 26.353 kb on - strand, within GFF5402 at 26.593 kb on - strand, within GFF5402 at 26.691 kb on + strand, within GFF5402 at 26.691 kb on + strand, within GFF5402 at 26.692 kb on - strand, within GFF5402 at 26.692 kb on - strand, within GFF5402 at 26.692 kb on - strand, within GFF5402 at 26.692 kb on - strand, within GFF5402 at 26.692 kb on - strand, within GFF5402 at 26.692 kb on - strand, within GFF5402 at 26.692 kb on - strand, within GFF5402 at 26.692 kb on - strand, within GFF5402 at 26.796 kb on + strand at 26.796 kb on + strand at 26.796 kb on + strand at 26.796 kb on + strand at 26.797 kb on - strand at 26.797 kb on - strand at 26.811 kb on + strand at 26.811 kb on + strand at 26.861 kb on + strand at 27.007 kb on + strand, within GFF5403 at 27.007 kb on + strand, within GFF5403 at 27.007 kb on + strand, within GFF5403 at 27.007 kb on + strand, within GFF5403 at 27.008 kb on - strand, within GFF5403 at 27.008 kb on - strand, within GFF5403 at 27.008 kb on - strand, within GFF5403 at 27.133 kb on + strand, within GFF5403 at 27.133 kb on + strand, within GFF5403 at 27.133 kb on + strand, within GFF5403 at 27.134 kb on - strand, within GFF5403 at 27.134 kb on - strand, within GFF5403 at 27.134 kb on - strand, within GFF5403 at 27.134 kb on - strand, within GFF5403 at 27.134 kb on - strand, within GFF5403 at 27.535 kb on + strand at 27.535 kb on + strand at 27.535 kb on + strand at 27.535 kb on + strand at 27.535 kb on + strand at 27.535 kb on + strand at 27.535 kb on + strand at 27.535 kb on + strand at 27.535 kb on + strand at 27.535 kb on + strand at 27.536 kb on - strand at 27.536 kb on - strand at 27.536 kb on - strand at 27.536 kb on - strand at 27.536 kb on - strand at 27.536 kb on - strand at 27.536 kb on - strand at 27.536 kb on - strand at 27.536 kb on - strand at 27.536 kb on - strand at 27.536 kb on - strand at 27.833 kb on + strand, within GFF5404 at 27.833 kb on + strand, within GFF5404 at 27.833 kb on + strand, within GFF5404 at 27.833 kb on + strand, within GFF5404 at 27.833 kb on + strand, within GFF5404 at 27.833 kb on + strand, within GFF5404 at 27.834 kb on - strand, within GFF5404 at 27.834 kb on - strand, within GFF5404 at 27.834 kb on - strand, within GFF5404 at 27.834 kb on - strand, within GFF5404 at 27.977 kb on + strand, within GFF5404 at 27.977 kb on + strand, within GFF5404 at 27.977 kb on + strand, within GFF5404 at 27.977 kb on + strand, within GFF5404 at 27.977 kb on + strand, within GFF5404 at 27.977 kb on + strand, within GFF5404 at 27.977 kb on + strand, within GFF5404 at 27.977 kb on + strand, within GFF5404 at 27.977 kb on + strand, within GFF5404 at 27.977 kb on + strand, within GFF5404 at 27.977 kb on + strand, within GFF5404 at 27.977 kb on + strand, within GFF5404 at 27.977 kb on + strand, within GFF5404 at 27.977 kb on + strand, within GFF5404 at 27.977 kb on + strand, within GFF5404 at 27.977 kb on + strand, within GFF5404 at 27.977 kb on + strand, within GFF5404 at 27.977 kb on + strand, within GFF5404 at 27.978 kb on - strand, within GFF5404 at 27.978 kb on - strand, within GFF5404 at 27.978 kb on - strand, within GFF5404 at 27.978 kb on - strand, within GFF5404 at 27.978 kb on - strand, within GFF5404 at 27.978 kb on - strand, within GFF5404 at 27.978 kb on - strand, within GFF5404 at 27.978 kb on - strand, within GFF5404 at 27.978 kb on - strand, within GFF5404 at 28.022 kb on + strand, within GFF5404 at 28.022 kb on + strand, within GFF5404 at 28.022 kb on + strand, within GFF5404 at 28.022 kb on + strand, within GFF5404 at 28.022 kb on + strand, within GFF5404 at 28.022 kb on + strand, within GFF5404 at 28.022 kb on + strand, within GFF5404 at 28.022 kb on + strand, within GFF5404 at 28.023 kb on - strand, within GFF5404 at 28.023 kb on - strand, within GFF5404 at 28.023 kb on - strand, within GFF5404 at 28.023 kb on - strand, within GFF5404 at 28.056 kb on - strand, within GFF5404 at 28.056 kb on - strand, within GFF5404 at 28.088 kb on + strand, within GFF5404 at 28.089 kb on - strand, within GFF5404 at 28.181 kb on + strand, within GFF5404 at 28.181 kb on + strand, within GFF5404 at 28.182 kb on - strand, within GFF5404 at 28.182 kb on - strand, within GFF5404 at 28.182 kb on - strand, within GFF5404 at 28.182 kb on - strand, within GFF5404 at 28.182 kb on - strand, within GFF5404 at 28.555 kb on + strand at 28.555 kb on + strand at 28.555 kb on + strand at 28.555 kb on + strand at 28.556 kb on - strand at 28.556 kb on - strand at 28.556 kb on - strand at 28.556 kb on - strand at 28.556 kb on - strand at 28.556 kb on - strand at 28.559 kb on + strand at 28.559 kb on + strand at 28.559 kb on + strand at 28.559 kb on + strand at 28.559 kb on + strand at 28.559 kb on + strand at 28.559 kb on + strand at 28.559 kb on + strand at 28.559 kb on + strand at 28.559 kb on + strand at 28.559 kb on + strand at 28.559 kb on + strand at 28.559 kb on + strand at 28.560 kb on - strand at 28.560 kb on - strand at 28.560 kb on - strand at 28.560 kb on - strand at 28.560 kb on - strand at 28.560 kb on - strand at 28.560 kb on - strand at 28.560 kb on - strand at 28.560 kb on - strand at 28.560 kb on - strand at 28.560 kb on - strand at 28.560 kb on - strand at 28.560 kb on - strand at 28.569 kb on + strand at 28.569 kb on + strand at 28.569 kb on + strand at 28.569 kb on + strand at 28.569 kb on + strand at 28.569 kb on + strand at 28.570 kb on - strand at 28.570 kb on - strand at 28.570 kb on - strand at 28.570 kb on - strand at 28.570 kb on - strand at 28.570 kb on - strand at 28.570 kb on - strand at 28.570 kb on - strand at 28.573 kb on + strand at 28.573 kb on + strand at 28.573 kb on + strand at 28.573 kb on + strand at 28.573 kb on + strand at 28.573 kb on + strand at 28.573 kb on + strand at 28.574 kb on - strand at 28.574 kb on - strand at 28.574 kb on - strand at 28.574 kb on - strand at 28.574 kb on - strand at 28.574 kb on - strand at 28.574 kb on - strand at 28.574 kb on - strand at 28.574 kb on - strand at 28.574 kb on - strand at 28.574 kb on - strand at 28.574 kb on - strand at 28.574 kb on - strand at 28.592 kb on + strand at 28.592 kb on + strand at 28.592 kb on + strand at 28.592 kb on + strand at 28.592 kb on + strand at 28.592 kb on + strand at 28.592 kb on + strand at 28.592 kb on + strand at 28.592 kb on + strand at 28.592 kb on + strand at 28.592 kb on + strand at 28.592 kb on + strand at 28.592 kb on + strand at 28.592 kb on + strand at 28.592 kb on + strand at 28.593 kb on - strand at 28.593 kb on - strand at 28.593 kb on - strand at 28.593 kb on - strand at 28.593 kb on - strand at 28.593 kb on - strand at 28.593 kb on - strand at 28.593 kb on - strand at 28.593 kb on - strand at 28.593 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 25,918 + -1.4 25,918 + -0.1 25,918 + -1.3 25,918 + +1.2 25,919 - -2.4 25,919 - +0.4 25,919 - -1.0 25,919 - -0.4 26,047 - GFF5402 0.10 -0.3 26,047 - GFF5402 0.10 +0.4 26,047 - GFF5402 0.10 -2.5 26,112 + GFF5402 0.18 -1.2 26,112 + GFF5402 0.18 +1.7 26,113 - GFF5402 0.18 +0.6 26,113 - GFF5402 0.18 -0.6 26,223 + GFF5402 0.31 +0.0 26,223 + GFF5402 0.31 -1.3 26,223 + GFF5402 0.31 +0.8 26,223 + GFF5402 0.31 +0.4 26,224 - GFF5402 0.31 +0.6 26,224 - GFF5402 0.31 -1.6 26,224 - GFF5402 0.31 -0.3 26,224 - GFF5402 0.31 -0.4 26,224 - GFF5402 0.31 -0.6 26,224 - GFF5402 0.31 -0.5 26,352 + GFF5402 0.46 +0.0 26,352 + GFF5402 0.46 +1.8 26,352 + GFF5402 0.46 -0.3 26,352 + GFF5402 0.46 -1.0 26,352 + GFF5402 0.46 +0.0 26,352 + GFF5402 0.46 +0.2 26,352 + GFF5402 0.46 -0.7 26,352 + GFF5402 0.46 +0.6 26,352 + GFF5402 0.46 -0.2 26,352 + GFF5402 0.46 +0.6 26,352 + GFF5402 0.46 +0.0 26,352 + GFF5402 0.46 +0.3 26,352 + GFF5402 0.46 -1.0 26,352 + GFF5402 0.46 -0.9 26,352 + GFF5402 0.46 -0.0 26,352 + GFF5402 0.46 -0.0 26,352 + GFF5402 0.46 -1.4 26,352 + GFF5402 0.46 +2.2 26,352 + GFF5402 0.46 +1.0 26,352 + GFF5402 0.46 -0.1 26,352 + GFF5402 0.46 +0.2 26,352 + GFF5402 0.46 -1.5 26,352 + GFF5402 0.46 -0.4 26,353 - GFF5402 0.46 -0.6 26,353 - GFF5402 0.46 -0.8 26,353 - GFF5402 0.46 -0.3 26,353 - GFF5402 0.46 -1.3 26,353 - GFF5402 0.46 -1.8 26,353 - GFF5402 0.46 -0.5 26,353 - GFF5402 0.46 +0.3 26,353 - GFF5402 0.46 -1.3 26,353 - GFF5402 0.46 +0.2 26,353 - GFF5402 0.46 -0.9 26,353 - GFF5402 0.46 -1.0 26,353 - GFF5402 0.46 +0.4 26,353 - GFF5402 0.46 -0.5 26,353 - GFF5402 0.46 -0.6 26,353 - GFF5402 0.46 -0.2 26,353 - GFF5402 0.46 -1.0 26,353 - GFF5402 0.46 -1.2 26,353 - GFF5402 0.46 -0.1 26,353 - GFF5402 0.46 -0.3 26,353 - GFF5402 0.46 -0.5 26,353 - GFF5402 0.46 -0.3 26,353 - GFF5402 0.46 -0.9 26,353 - GFF5402 0.46 -0.3 26,353 - GFF5402 0.46 -0.1 26,353 - GFF5402 0.46 -1.0 26,353 - GFF5402 0.46 -0.8 26,353 - GFF5402 0.46 -0.4 26,353 - GFF5402 0.46 -0.5 26,353 - GFF5402 0.46 +0.0 26,593 - GFF5402 0.74 +0.3 26,691 + GFF5402 0.86 -0.2 26,691 + GFF5402 0.86 -1.1 26,692 - GFF5402 0.86 +1.2 26,692 - GFF5402 0.86 -0.4 26,692 - GFF5402 0.86 -0.5 26,692 - GFF5402 0.86 -2.0 26,692 - GFF5402 0.86 +0.8 26,692 - GFF5402 0.86 -0.3 26,692 - GFF5402 0.86 -0.5 26,692 - GFF5402 0.86 -1.3 26,796 + -0.6 26,796 + -0.1 26,796 + -0.7 26,796 + -0.2 26,797 - +0.6 26,797 - -0.3 26,811 + -0.3 26,811 + -0.7 26,861 + -1.0 27,007 + GFF5403 0.18 +0.6 27,007 + GFF5403 0.18 -0.0 27,007 + GFF5403 0.18 +0.8 27,007 + GFF5403 0.18 +0.4 27,008 - GFF5403 0.18 -1.0 27,008 - GFF5403 0.18 +0.8 27,008 - GFF5403 0.18 -0.1 27,133 + GFF5403 0.36 +0.4 27,133 + GFF5403 0.36 -0.5 27,133 + GFF5403 0.36 +0.8 27,134 - GFF5403 0.36 +0.2 27,134 - GFF5403 0.36 +0.3 27,134 - GFF5403 0.36 +1.2 27,134 - GFF5403 0.36 +0.5 27,134 - GFF5403 0.36 +2.8 27,535 + -0.3 27,535 + -0.3 27,535 + +0.2 27,535 + -0.1 27,535 + -1.0 27,535 + +0.8 27,535 + +1.2 27,535 + -0.1 27,535 + -0.6 27,535 + +0.4 27,536 - +0.4 27,536 - -1.0 27,536 - +0.3 27,536 - -0.5 27,536 - -1.4 27,536 - -0.3 27,536 - +0.4 27,536 - +0.4 27,536 - -1.5 27,536 - -2.1 27,536 - -0.3 27,833 + GFF5404 0.24 +0.4 27,833 + GFF5404 0.24 -0.2 27,833 + GFF5404 0.24 -0.8 27,833 + GFF5404 0.24 -0.1 27,833 + GFF5404 0.24 -0.0 27,833 + GFF5404 0.24 -0.5 27,834 - GFF5404 0.24 +0.8 27,834 - GFF5404 0.24 +2.6 27,834 - GFF5404 0.24 +0.6 27,834 - GFF5404 0.24 -1.2 27,977 + GFF5404 0.39 +0.2 27,977 + GFF5404 0.39 -0.0 27,977 + GFF5404 0.39 +2.4 27,977 + GFF5404 0.39 +0.3 27,977 + GFF5404 0.39 +0.0 27,977 + GFF5404 0.39 -0.2 27,977 + GFF5404 0.39 +0.3 27,977 + GFF5404 0.39 -0.7 27,977 + GFF5404 0.39 +0.4 27,977 + GFF5404 0.39 +1.0 27,977 + GFF5404 0.39 -0.1 27,977 + GFF5404 0.39 -1.2 27,977 + GFF5404 0.39 +0.2 27,977 + GFF5404 0.39 -0.6 27,977 + GFF5404 0.39 +0.2 27,977 + GFF5404 0.39 +0.3 27,977 + GFF5404 0.39 -0.2 27,977 + GFF5404 0.39 +2.1 27,978 - GFF5404 0.40 +0.4 27,978 - GFF5404 0.40 +0.2 27,978 - GFF5404 0.40 -0.6 27,978 - GFF5404 0.40 -0.8 27,978 - GFF5404 0.40 +0.4 27,978 - GFF5404 0.40 -2.0 27,978 - GFF5404 0.40 -1.6 27,978 - GFF5404 0.40 -0.8 27,978 - GFF5404 0.40 -1.2 28,022 + GFF5404 0.44 +1.0 28,022 + GFF5404 0.44 -1.1 28,022 + GFF5404 0.44 +1.2 28,022 + GFF5404 0.44 -0.1 28,022 + GFF5404 0.44 +1.6 28,022 + GFF5404 0.44 -1.1 28,022 + GFF5404 0.44 +0.4 28,022 + GFF5404 0.44 -1.0 28,023 - GFF5404 0.44 -0.1 28,023 - GFF5404 0.44 +0.6 28,023 - GFF5404 0.44 -0.2 28,023 - GFF5404 0.44 -1.3 28,056 - GFF5404 0.48 -0.4 28,056 - GFF5404 0.48 +0.8 28,088 + GFF5404 0.51 -0.8 28,089 - GFF5404 0.52 -1.1 28,181 + GFF5404 0.61 -0.8 28,181 + GFF5404 0.61 -0.5 28,182 - GFF5404 0.62 -0.5 28,182 - GFF5404 0.62 -0.5 28,182 - GFF5404 0.62 -0.9 28,182 - GFF5404 0.62 -2.6 28,182 - GFF5404 0.62 +0.1 28,555 + +0.2 28,555 + -0.8 28,555 + +0.2 28,555 + +0.4 28,556 - +0.0 28,556 - +1.0 28,556 - -0.5 28,556 - -0.1 28,556 - -0.6 28,556 - -0.4 28,559 + +0.2 28,559 + -0.2 28,559 + -0.2 28,559 + +0.6 28,559 + -1.0 28,559 + -0.2 28,559 + -1.3 28,559 + +0.9 28,559 + -0.7 28,559 + -0.6 28,559 + +0.0 28,559 + +0.9 28,559 + -1.3 28,560 - -0.4 28,560 - -0.3 28,560 - -1.2 28,560 - -0.2 28,560 - -1.5 28,560 - -1.1 28,560 - -0.1 28,560 - -1.6 28,560 - -0.6 28,560 - +1.7 28,560 - +0.3 28,560 - +0.5 28,560 - -1.4 28,569 + +0.8 28,569 + +0.0 28,569 + -1.0 28,569 + -0.2 28,569 + -0.6 28,569 + -0.4 28,570 - -0.2 28,570 - -0.2 28,570 - +0.3 28,570 - +0.1 28,570 - -0.2 28,570 - -0.4 28,570 - +0.7 28,570 - -0.1 28,573 + -0.7 28,573 + +1.0 28,573 + +0.7 28,573 + -0.0 28,573 + -0.8 28,573 + -0.2 28,573 + +0.4 28,574 - +0.2 28,574 - +1.1 28,574 - +0.1 28,574 - +0.2 28,574 - +0.2 28,574 - +1.5 28,574 - -0.4 28,574 - -0.1 28,574 - +0.3 28,574 - +0.1 28,574 - -1.0 28,574 - +0.6 28,574 - -1.1 28,592 + -0.9 28,592 + +1.0 28,592 + -0.2 28,592 + -1.8 28,592 + -0.8 28,592 + -1.4 28,592 + +0.9 28,592 + -1.8 28,592 + -0.8 28,592 + -1.5 28,592 + -0.7 28,592 + -0.1 28,592 + -0.3 28,592 + -0.3 28,592 + +0.0 28,593 - -0.7 28,593 - +0.1 28,593 - -1.0 28,593 - +0.0 28,593 - -1.2 28,593 - -0.1 28,593 - -0.1 28,593 - -0.1 28,593 - +1.5 28,593 - -0.6
Or see this region's nucleotide sequence