Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5315 and GFF5316 overlap by 4 nucleotides GFF5316 and GFF5317 are separated by 63 nucleotides GFF5317 and GFF5318 are separated by 9 nucleotides
GFF5315 - Diaminopimelate decarboxylase (EC 4.1.1.20), at 192,174 to 193,463
GFF5315
GFF5316 - Diaminopimelate decarboxylase and/or diaminopimelate epimerase leader peptide, at 193,460 to 193,687
GFF5316
GFF5317 - Frataxin homolog CyaY, facilitates Fe-S cluster assembly, interacts with IscS, at 193,751 to 194,083
GFF5317
GFF5318 - Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-), at 194,093 to 196,504
GFF5318
Position (kb) 193
194
195 Strain fitness (log2 ratio) -2
-1
0
1
2 at 193.627 kb on - strand, within GFF5316 at 193.716 kb on - strand at 193.716 kb on - strand at 193.738 kb on + strand at 193.739 kb on - strand at 193.749 kb on + strand at 193.750 kb on - strand at 193.750 kb on - strand at 193.766 kb on + strand at 193.766 kb on + strand at 193.766 kb on + strand at 193.766 kb on + strand at 193.766 kb on + strand at 193.767 kb on - strand at 193.767 kb on - strand at 193.767 kb on - strand at 193.767 kb on - strand at 193.767 kb on - strand at 193.767 kb on - strand at 193.767 kb on - strand at 193.767 kb on - strand at 193.767 kb on - strand at 193.767 kb on - strand at 193.767 kb on - strand at 193.962 kb on - strand, within GFF5317 at 193.968 kb on - strand, within GFF5317 at 194.232 kb on + strand at 194.232 kb on + strand at 194.233 kb on - strand at 194.241 kb on + strand at 194.241 kb on + strand at 194.241 kb on + strand at 194.241 kb on + strand at 194.241 kb on + strand at 194.241 kb on + strand at 194.241 kb on + strand at 194.241 kb on + strand at 194.242 kb on - strand at 194.242 kb on - strand at 194.242 kb on - strand at 194.242 kb on - strand at 194.242 kb on - strand at 194.242 kb on - strand at 194.242 kb on - strand at 194.244 kb on + strand at 194.244 kb on + strand at 194.244 kb on + strand at 194.244 kb on + strand at 194.244 kb on + strand at 194.245 kb on - strand at 194.331 kb on + strand at 194.331 kb on + strand at 194.331 kb on + strand at 194.331 kb on + strand at 194.331 kb on + strand at 194.331 kb on + strand at 194.331 kb on + strand at 194.331 kb on + strand at 194.331 kb on + strand at 194.331 kb on + strand at 194.332 kb on - strand at 194.332 kb on - strand at 194.332 kb on - strand at 194.332 kb on - strand at 194.332 kb on - strand at 194.332 kb on - strand at 194.332 kb on - strand at 194.332 kb on - strand at 194.332 kb on - strand at 194.332 kb on - strand at 194.332 kb on - strand at 194.401 kb on - strand, within GFF5318 at 194.487 kb on + strand, within GFF5318 at 194.488 kb on - strand, within GFF5318 at 194.488 kb on - strand, within GFF5318 at 194.673 kb on + strand, within GFF5318 at 194.674 kb on - strand, within GFF5318 at 194.674 kb on - strand, within GFF5318 at 194.674 kb on - strand, within GFF5318 at 194.688 kb on + strand, within GFF5318 at 194.769 kb on + strand, within GFF5318 at 194.769 kb on + strand, within GFF5318 at 194.769 kb on + strand, within GFF5318 at 194.770 kb on - strand, within GFF5318 at 194.823 kb on + strand, within GFF5318 at 194.823 kb on + strand, within GFF5318 at 194.824 kb on - strand, within GFF5318 at 194.824 kb on - strand, within GFF5318 at 194.824 kb on - strand, within GFF5318 at 194.911 kb on - strand, within GFF5318 at 194.922 kb on + strand, within GFF5318 at 194.922 kb on + strand, within GFF5318 at 194.922 kb on + strand, within GFF5318 at 194.923 kb on - strand, within GFF5318 at 194.923 kb on - strand, within GFF5318 at 194.923 kb on - strand, within GFF5318 at 194.923 kb on - strand, within GFF5318 at 194.923 kb on - strand, within GFF5318 at 194.923 kb on - strand, within GFF5318 at 194.923 kb on - strand, within GFF5318 at 195.030 kb on + strand, within GFF5318 at 195.031 kb on - strand, within GFF5318 at 195.031 kb on - strand, within GFF5318 at 195.031 kb on - strand, within GFF5318 at 195.031 kb on - strand, within GFF5318 at 195.031 kb on - strand, within GFF5318 at 195.031 kb on - strand, within GFF5318 at 195.031 kb on - strand, within GFF5318 at 195.031 kb on - strand, within GFF5318 at 195.031 kb on - strand, within GFF5318
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 193,627 - GFF5316 0.73 +0.6 193,716 - +0.8 193,716 - +0.8 193,738 + +1.0 193,739 - +0.5 193,749 + -0.6 193,750 - -0.9 193,750 - -0.4 193,766 + -1.9 193,766 + -1.2 193,766 + +0.0 193,766 + +0.4 193,766 + -0.7 193,767 - -0.5 193,767 - -0.4 193,767 - -0.2 193,767 - -0.4 193,767 - +0.9 193,767 - -0.8 193,767 - -0.3 193,767 - -0.2 193,767 - -1.1 193,767 - -1.7 193,767 - -1.6 193,962 - GFF5317 0.63 -1.7 193,968 - GFF5317 0.65 -1.2 194,232 + -1.5 194,232 + +0.0 194,233 - -1.2 194,241 + -0.9 194,241 + -0.6 194,241 + +0.1 194,241 + +0.1 194,241 + -0.4 194,241 + -0.1 194,241 + -0.2 194,241 + +0.4 194,242 - +0.4 194,242 - +0.6 194,242 - +0.3 194,242 - -0.4 194,242 - +0.4 194,242 - +0.7 194,242 - -0.8 194,244 + -1.2 194,244 + +0.2 194,244 + +0.1 194,244 + +1.1 194,244 + -0.4 194,245 - +1.0 194,331 + -0.2 194,331 + +2.4 194,331 + -1.6 194,331 + -0.8 194,331 + -0.4 194,331 + -1.0 194,331 + -0.1 194,331 + -0.2 194,331 + -0.9 194,331 + -1.0 194,332 - -0.5 194,332 - +1.0 194,332 - -0.5 194,332 - +0.1 194,332 - -0.3 194,332 - +0.6 194,332 - +0.5 194,332 - -0.7 194,332 - -1.2 194,332 - +0.0 194,332 - +0.6 194,401 - GFF5318 0.13 -1.7 194,487 + GFF5318 0.16 -0.4 194,488 - GFF5318 0.16 +0.2 194,488 - GFF5318 0.16 +0.2 194,673 + GFF5318 0.24 -0.6 194,674 - GFF5318 0.24 +0.6 194,674 - GFF5318 0.24 +0.6 194,674 - GFF5318 0.24 +0.2 194,688 + GFF5318 0.25 -0.1 194,769 + GFF5318 0.28 -0.0 194,769 + GFF5318 0.28 -0.1 194,769 + GFF5318 0.28 +0.4 194,770 - GFF5318 0.28 -1.0 194,823 + GFF5318 0.30 -0.4 194,823 + GFF5318 0.30 -1.2 194,824 - GFF5318 0.30 -1.7 194,824 - GFF5318 0.30 +0.7 194,824 - GFF5318 0.30 +2.6 194,911 - GFF5318 0.34 +0.6 194,922 + GFF5318 0.34 -0.0 194,922 + GFF5318 0.34 +0.2 194,922 + GFF5318 0.34 -0.5 194,923 - GFF5318 0.34 +0.1 194,923 - GFF5318 0.34 +0.8 194,923 - GFF5318 0.34 +0.0 194,923 - GFF5318 0.34 -1.7 194,923 - GFF5318 0.34 -1.6 194,923 - GFF5318 0.34 +0.8 194,923 - GFF5318 0.34 -0.2 195,030 + GFF5318 0.39 +0.6 195,031 - GFF5318 0.39 -1.2 195,031 - GFF5318 0.39 +0.2 195,031 - GFF5318 0.39 -0.4 195,031 - GFF5318 0.39 -0.1 195,031 - GFF5318 0.39 +0.3 195,031 - GFF5318 0.39 -0.4 195,031 - GFF5318 0.39 +0.3 195,031 - GFF5318 0.39 -0.4 195,031 - GFF5318 0.39 -0.2
Or see this region's nucleotide sequence