Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5276 and GFF5277 overlap by 4 nucleotides GFF5277 and GFF5278 are separated by 22 nucleotides GFF5278 and GFF5279 are separated by 30 nucleotides
GFF5276 - D-arabinitol 4-dehydrogenase (EC 1.1.1.11), at 144,433 to 145,869
GFF5276
GFF5277 - Sorbitol dehydrogenase (EC 1.1.1.14), at 145,866 to 146,654
GFF5277
GFF5278 - Transaldolase (EC 2.2.1.2), at 146,677 to 147,348
GFF5278
GFF5279 - Fructokinase (EC 2.7.1.4), at 147,379 to 148,347
GFF5279
Position (kb) 146
147
148 Strain fitness (log2 ratio) -2
-1
0
1 at 145.678 kb on + strand, within GFF5276 at 145.679 kb on - strand, within GFF5276 at 145.679 kb on - strand, within GFF5276 at 146.043 kb on + strand, within GFF5277 at 146.043 kb on + strand, within GFF5277 at 146.043 kb on + strand, within GFF5277 at 146.044 kb on - strand, within GFF5277 at 146.044 kb on - strand, within GFF5277 at 146.044 kb on - strand, within GFF5277 at 146.044 kb on - strand, within GFF5277 at 146.044 kb on - strand, within GFF5277 at 146.337 kb on + strand, within GFF5277 at 146.337 kb on + strand, within GFF5277 at 146.337 kb on + strand, within GFF5277 at 146.367 kb on + strand, within GFF5277 at 146.367 kb on + strand, within GFF5277 at 146.367 kb on + strand, within GFF5277 at 146.367 kb on + strand, within GFF5277 at 146.367 kb on + strand, within GFF5277 at 146.368 kb on - strand, within GFF5277 at 146.368 kb on - strand, within GFF5277 at 146.368 kb on - strand, within GFF5277 at 146.368 kb on - strand, within GFF5277 at 146.368 kb on - strand, within GFF5277 at 146.368 kb on - strand, within GFF5277 at 146.407 kb on + strand, within GFF5277 at 146.407 kb on + strand, within GFF5277 at 146.408 kb on - strand, within GFF5277 at 146.408 kb on - strand, within GFF5277 at 146.604 kb on + strand at 146.605 kb on - strand at 146.698 kb on + strand at 146.699 kb on - strand at 146.699 kb on - strand at 146.699 kb on - strand at 146.699 kb on - strand at 147.031 kb on + strand, within GFF5278 at 147.032 kb on - strand, within GFF5278 at 147.077 kb on - strand, within GFF5278 at 147.077 kb on - strand, within GFF5278 at 147.088 kb on + strand, within GFF5278 at 147.217 kb on + strand, within GFF5278 at 147.217 kb on + strand, within GFF5278 at 147.218 kb on - strand, within GFF5278 at 147.218 kb on - strand, within GFF5278 at 147.218 kb on - strand, within GFF5278 at 147.218 kb on - strand, within GFF5278 at 147.661 kb on + strand, within GFF5279 at 147.662 kb on - strand, within GFF5279 at 147.670 kb on + strand, within GFF5279 at 147.670 kb on + strand, within GFF5279 at 147.670 kb on + strand, within GFF5279 at 147.670 kb on + strand, within GFF5279 at 147.670 kb on + strand, within GFF5279 at 147.671 kb on - strand, within GFF5279 at 147.671 kb on - strand, within GFF5279 at 147.671 kb on - strand, within GFF5279 at 147.671 kb on - strand, within GFF5279 at 147.671 kb on - strand, within GFF5279 at 147.880 kb on + strand, within GFF5279 at 148.074 kb on + strand, within GFF5279 at 148.074 kb on + strand, within GFF5279 at 148.075 kb on - strand, within GFF5279 at 148.075 kb on - strand, within GFF5279 at 148.323 kb on + strand at 148.324 kb on - strand at 148.324 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 145,678 + GFF5276 0.87 -2.0 145,679 - GFF5276 0.87 -0.1 145,679 - GFF5276 0.87 +0.2 146,043 + GFF5277 0.22 -0.5 146,043 + GFF5277 0.22 +0.1 146,043 + GFF5277 0.22 +0.6 146,044 - GFF5277 0.23 -0.1 146,044 - GFF5277 0.23 +0.2 146,044 - GFF5277 0.23 +0.0 146,044 - GFF5277 0.23 -1.0 146,044 - GFF5277 0.23 +0.4 146,337 + GFF5277 0.60 -1.5 146,337 + GFF5277 0.60 +0.2 146,337 + GFF5277 0.60 +0.8 146,367 + GFF5277 0.63 +0.3 146,367 + GFF5277 0.63 +0.3 146,367 + GFF5277 0.63 -0.2 146,367 + GFF5277 0.63 +1.0 146,367 + GFF5277 0.63 -0.2 146,368 - GFF5277 0.64 +0.3 146,368 - GFF5277 0.64 -2.2 146,368 - GFF5277 0.64 -2.1 146,368 - GFF5277 0.64 -1.6 146,368 - GFF5277 0.64 -0.1 146,368 - GFF5277 0.64 +0.5 146,407 + GFF5277 0.69 -0.4 146,407 + GFF5277 0.69 +0.6 146,408 - GFF5277 0.69 -0.3 146,408 - GFF5277 0.69 -0.4 146,604 + -0.7 146,605 - +0.8 146,698 + +0.0 146,699 - +0.8 146,699 - +0.1 146,699 - +0.2 146,699 - -0.9 147,031 + GFF5278 0.53 -1.2 147,032 - GFF5278 0.53 +0.5 147,077 - GFF5278 0.60 -0.4 147,077 - GFF5278 0.60 -2.2 147,088 + GFF5278 0.61 -0.9 147,217 + GFF5278 0.80 -0.3 147,217 + GFF5278 0.80 -0.6 147,218 - GFF5278 0.81 -1.2 147,218 - GFF5278 0.81 -1.3 147,218 - GFF5278 0.81 -0.6 147,218 - GFF5278 0.81 +0.8 147,661 + GFF5279 0.29 -0.4 147,662 - GFF5279 0.29 -0.2 147,670 + GFF5279 0.30 -0.1 147,670 + GFF5279 0.30 +0.0 147,670 + GFF5279 0.30 -1.0 147,670 + GFF5279 0.30 -1.6 147,670 + GFF5279 0.30 -1.7 147,671 - GFF5279 0.30 -1.7 147,671 - GFF5279 0.30 -0.4 147,671 - GFF5279 0.30 -1.3 147,671 - GFF5279 0.30 +0.2 147,671 - GFF5279 0.30 +0.0 147,880 + GFF5279 0.52 +0.0 148,074 + GFF5279 0.72 +1.6 148,074 + GFF5279 0.72 -0.1 148,075 - GFF5279 0.72 -0.9 148,075 - GFF5279 0.72 +0.6 148,323 + -0.6 148,324 - -0.3 148,324 - -1.1
Or see this region's nucleotide sequence