Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5253 and GFF5254 are separated by 31 nucleotides GFF5254 and GFF5255 are separated by 157 nucleotides
GFF5253 - Amidohydrolase family protein, at 117,498 to 118,769
GFF5253
GFF5254 - ABC transporter, substrate-binding protein (cluster 5, nickel/peptides/opines), at 118,801 to 120,384
GFF5254
GFF5255 - ABC transporter, permease protein 2 (cluster 5, nickel/peptides/opines), at 120,542 to 121,453
GFF5255
Position (kb)
118
119
120
121 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 117.895 kb on - strand, within GFF5253 at 118.084 kb on - strand, within GFF5253 at 118.149 kb on + strand, within GFF5253 at 118.150 kb on - strand, within GFF5253 at 118.425 kb on + strand, within GFF5253 at 118.425 kb on + strand, within GFF5253 at 118.425 kb on + strand, within GFF5253 at 118.425 kb on + strand, within GFF5253 at 118.425 kb on + strand, within GFF5253 at 118.426 kb on - strand, within GFF5253 at 118.426 kb on - strand, within GFF5253 at 118.426 kb on - strand, within GFF5253 at 118.426 kb on - strand, within GFF5253 at 118.426 kb on - strand, within GFF5253 at 118.426 kb on - strand, within GFF5253 at 118.437 kb on + strand, within GFF5253 at 118.437 kb on + strand, within GFF5253 at 118.437 kb on + strand, within GFF5253 at 118.438 kb on - strand, within GFF5253 at 118.438 kb on - strand, within GFF5253 at 118.587 kb on + strand, within GFF5253 at 118.588 kb on - strand, within GFF5253 at 118.588 kb on - strand, within GFF5253 at 118.641 kb on + strand, within GFF5253 at 118.911 kb on - strand at 118.969 kb on + strand, within GFF5254 at 118.969 kb on + strand, within GFF5254 at 118.969 kb on + strand, within GFF5254 at 118.970 kb on - strand, within GFF5254 at 118.970 kb on - strand, within GFF5254 at 118.970 kb on - strand, within GFF5254 at 118.970 kb on - strand, within GFF5254 at 119.339 kb on - strand, within GFF5254 at 119.434 kb on + strand, within GFF5254 at 119.434 kb on + strand, within GFF5254 at 119.434 kb on + strand, within GFF5254 at 119.434 kb on + strand, within GFF5254 at 119.434 kb on + strand, within GFF5254 at 119.435 kb on - strand, within GFF5254 at 119.659 kb on + strand, within GFF5254 at 119.659 kb on + strand, within GFF5254 at 119.660 kb on - strand, within GFF5254 at 119.660 kb on - strand, within GFF5254 at 119.716 kb on + strand, within GFF5254 at 119.716 kb on + strand, within GFF5254 at 119.717 kb on - strand, within GFF5254 at 119.717 kb on - strand, within GFF5254 at 119.717 kb on - strand, within GFF5254 at 119.717 kb on - strand, within GFF5254 at 119.839 kb on + strand, within GFF5254 at 119.839 kb on + strand, within GFF5254 at 119.840 kb on - strand, within GFF5254 at 119.840 kb on - strand, within GFF5254 at 119.926 kb on + strand, within GFF5254 at 119.927 kb on - strand, within GFF5254 at 120.019 kb on + strand, within GFF5254 at 120.019 kb on + strand, within GFF5254 at 120.019 kb on + strand, within GFF5254 at 120.019 kb on + strand, within GFF5254 at 120.019 kb on + strand, within GFF5254 at 120.019 kb on + strand, within GFF5254 at 120.020 kb on - strand, within GFF5254 at 120.020 kb on - strand, within GFF5254 at 120.020 kb on - strand, within GFF5254 at 120.020 kb on - strand, within GFF5254 at 120.020 kb on - strand, within GFF5254 at 120.020 kb on - strand, within GFF5254 at 120.020 kb on - strand, within GFF5254 at 120.020 kb on - strand, within GFF5254 at 120.020 kb on - strand, within GFF5254 at 120.020 kb on - strand, within GFF5254 at 120.055 kb on + strand, within GFF5254 at 120.055 kb on + strand, within GFF5254 at 120.055 kb on + strand, within GFF5254 at 120.055 kb on + strand, within GFF5254 at 120.055 kb on + strand, within GFF5254 at 120.056 kb on - strand, within GFF5254 at 120.056 kb on - strand, within GFF5254 at 120.056 kb on - strand, within GFF5254 at 120.056 kb on - strand, within GFF5254 at 120.056 kb on - strand, within GFF5254 at 120.056 kb on - strand, within GFF5254 at 120.056 kb on - strand, within GFF5254 at 120.056 kb on - strand, within GFF5254 at 120.082 kb on + strand, within GFF5254 at 120.082 kb on + strand, within GFF5254 at 120.082 kb on + strand, within GFF5254 at 120.082 kb on + strand, within GFF5254 at 120.082 kb on + strand, within GFF5254 at 120.083 kb on - strand, within GFF5254 at 120.083 kb on - strand, within GFF5254 at 120.083 kb on - strand, within GFF5254 at 120.083 kb on - strand, within GFF5254 at 120.083 kb on - strand, within GFF5254 at 120.160 kb on + strand, within GFF5254 at 120.160 kb on + strand, within GFF5254 at 120.355 kb on + strand at 120.355 kb on + strand at 120.355 kb on + strand at 120.355 kb on + strand at 120.356 kb on - strand at 120.356 kb on - strand at 120.356 kb on - strand at 120.356 kb on - strand at 120.356 kb on - strand at 120.464 kb on + strand at 120.464 kb on + strand at 120.464 kb on + strand at 120.465 kb on - strand at 120.758 kb on + strand, within GFF5255 at 120.758 kb on + strand, within GFF5255 at 120.758 kb on + strand, within GFF5255 at 120.759 kb on - strand, within GFF5255 at 120.809 kb on + strand, within GFF5255 at 120.809 kb on + strand, within GFF5255 at 120.809 kb on + strand, within GFF5255 at 120.809 kb on + strand, within GFF5255 at 120.809 kb on + strand, within GFF5255 at 120.809 kb on + strand, within GFF5255 at 120.810 kb on - strand, within GFF5255 at 120.810 kb on - strand, within GFF5255 at 120.810 kb on - strand, within GFF5255 at 120.933 kb on + strand, within GFF5255 at 120.933 kb on + strand, within GFF5255 at 120.934 kb on - strand, within GFF5255 at 121.077 kb on - strand, within GFF5255 at 121.077 kb on - strand, within GFF5255 at 121.077 kb on - strand, within GFF5255 at 121.124 kb on + strand, within GFF5255 at 121.124 kb on + strand, within GFF5255 at 121.124 kb on + strand, within GFF5255 at 121.124 kb on + strand, within GFF5255 at 121.124 kb on + strand, within GFF5255 at 121.124 kb on + strand, within GFF5255 at 121.125 kb on - strand, within GFF5255 at 121.125 kb on - strand, within GFF5255 at 121.125 kb on - strand, within GFF5255 at 121.125 kb on - strand, within GFF5255 at 121.125 kb on - strand, within GFF5255 at 121.125 kb on - strand, within GFF5255 at 121.125 kb on - strand, within GFF5255 at 121.125 kb on - strand, within GFF5255 at 121.125 kb on - strand, within GFF5255 at 121.331 kb on + strand, within GFF5255 at 121.331 kb on + strand, within GFF5255 at 121.332 kb on - strand, within GFF5255 at 121.332 kb on - strand, within GFF5255 at 121.332 kb on - strand, within GFF5255
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 117,895 - GFF5253 0.31 +0.3 118,084 - GFF5253 0.46 -1.0 118,149 + GFF5253 0.51 +2.0 118,150 - GFF5253 0.51 -0.1 118,425 + GFF5253 0.73 -0.6 118,425 + GFF5253 0.73 -1.4 118,425 + GFF5253 0.73 -1.7 118,425 + GFF5253 0.73 -0.5 118,425 + GFF5253 0.73 -0.7 118,426 - GFF5253 0.73 -0.1 118,426 - GFF5253 0.73 +0.0 118,426 - GFF5253 0.73 -0.5 118,426 - GFF5253 0.73 +2.3 118,426 - GFF5253 0.73 -0.1 118,426 - GFF5253 0.73 +0.6 118,437 + GFF5253 0.74 +0.3 118,437 + GFF5253 0.74 -1.0 118,437 + GFF5253 0.74 +1.1 118,438 - GFF5253 0.74 +0.1 118,438 - GFF5253 0.74 -2.0 118,587 + GFF5253 0.86 +0.2 118,588 - GFF5253 0.86 +1.1 118,588 - GFF5253 0.86 +0.3 118,641 + GFF5253 0.90 -0.3 118,911 - -0.6 118,969 + GFF5254 0.11 +0.5 118,969 + GFF5254 0.11 -0.2 118,969 + GFF5254 0.11 -1.4 118,970 - GFF5254 0.11 +0.6 118,970 - GFF5254 0.11 +0.4 118,970 - GFF5254 0.11 +0.0 118,970 - GFF5254 0.11 -1.3 119,339 - GFF5254 0.34 +0.3 119,434 + GFF5254 0.40 +1.6 119,434 + GFF5254 0.40 -0.1 119,434 + GFF5254 0.40 +1.0 119,434 + GFF5254 0.40 +0.6 119,434 + GFF5254 0.40 +0.0 119,435 - GFF5254 0.40 -0.1 119,659 + GFF5254 0.54 +0.5 119,659 + GFF5254 0.54 +0.9 119,660 - GFF5254 0.54 +1.2 119,660 - GFF5254 0.54 -0.3 119,716 + GFF5254 0.58 +0.0 119,716 + GFF5254 0.58 +0.3 119,717 - GFF5254 0.58 +0.6 119,717 - GFF5254 0.58 -0.6 119,717 - GFF5254 0.58 +0.2 119,717 - GFF5254 0.58 +0.1 119,839 + GFF5254 0.66 -0.2 119,839 + GFF5254 0.66 -2.0 119,840 - GFF5254 0.66 +0.1 119,840 - GFF5254 0.66 -0.2 119,926 + GFF5254 0.71 +0.0 119,927 - GFF5254 0.71 +0.3 120,019 + GFF5254 0.77 -0.2 120,019 + GFF5254 0.77 -2.2 120,019 + GFF5254 0.77 -1.7 120,019 + GFF5254 0.77 +0.0 120,019 + GFF5254 0.77 -0.7 120,019 + GFF5254 0.77 +0.2 120,020 - GFF5254 0.77 +0.6 120,020 - GFF5254 0.77 -0.6 120,020 - GFF5254 0.77 -0.3 120,020 - GFF5254 0.77 -1.9 120,020 - GFF5254 0.77 +1.6 120,020 - GFF5254 0.77 -1.3 120,020 - GFF5254 0.77 +0.6 120,020 - GFF5254 0.77 -1.1 120,020 - GFF5254 0.77 -0.3 120,020 - GFF5254 0.77 -0.3 120,055 + GFF5254 0.79 -0.2 120,055 + GFF5254 0.79 -0.0 120,055 + GFF5254 0.79 -0.1 120,055 + GFF5254 0.79 +1.3 120,055 + GFF5254 0.79 +0.3 120,056 - GFF5254 0.79 -0.4 120,056 - GFF5254 0.79 -2.1 120,056 - GFF5254 0.79 +2.3 120,056 - GFF5254 0.79 -0.1 120,056 - GFF5254 0.79 +0.6 120,056 - GFF5254 0.79 -0.8 120,056 - GFF5254 0.79 -0.0 120,056 - GFF5254 0.79 +0.7 120,082 + GFF5254 0.81 -0.7 120,082 + GFF5254 0.81 -0.3 120,082 + GFF5254 0.81 +0.6 120,082 + GFF5254 0.81 -0.4 120,082 + GFF5254 0.81 +0.1 120,083 - GFF5254 0.81 +0.3 120,083 - GFF5254 0.81 +0.5 120,083 - GFF5254 0.81 +0.1 120,083 - GFF5254 0.81 -1.2 120,083 - GFF5254 0.81 -0.5 120,160 + GFF5254 0.86 +0.4 120,160 + GFF5254 0.86 +0.3 120,355 + +0.7 120,355 + -0.0 120,355 + +0.4 120,355 + +0.2 120,356 - -0.5 120,356 - -1.2 120,356 - +0.6 120,356 - -2.6 120,356 - -0.2 120,464 + -0.5 120,464 + -0.1 120,464 + -0.3 120,465 - -0.5 120,758 + GFF5255 0.24 +0.1 120,758 + GFF5255 0.24 +1.2 120,758 + GFF5255 0.24 -2.4 120,759 - GFF5255 0.24 -2.4 120,809 + GFF5255 0.29 -0.1 120,809 + GFF5255 0.29 +1.2 120,809 + GFF5255 0.29 +1.3 120,809 + GFF5255 0.29 -1.0 120,809 + GFF5255 0.29 +0.1 120,809 + GFF5255 0.29 -0.9 120,810 - GFF5255 0.29 +0.7 120,810 - GFF5255 0.29 -0.1 120,810 - GFF5255 0.29 +0.1 120,933 + GFF5255 0.43 -0.5 120,933 + GFF5255 0.43 -0.4 120,934 - GFF5255 0.43 +0.3 121,077 - GFF5255 0.59 -1.1 121,077 - GFF5255 0.59 +1.1 121,077 - GFF5255 0.59 +0.8 121,124 + GFF5255 0.64 -0.7 121,124 + GFF5255 0.64 -0.6 121,124 + GFF5255 0.64 -0.1 121,124 + GFF5255 0.64 +0.3 121,124 + GFF5255 0.64 -0.4 121,124 + GFF5255 0.64 +0.2 121,125 - GFF5255 0.64 +0.4 121,125 - GFF5255 0.64 -0.3 121,125 - GFF5255 0.64 -1.6 121,125 - GFF5255 0.64 +2.3 121,125 - GFF5255 0.64 -0.5 121,125 - GFF5255 0.64 -0.8 121,125 - GFF5255 0.64 +0.0 121,125 - GFF5255 0.64 -0.6 121,125 - GFF5255 0.64 +0.2 121,331 + GFF5255 0.87 -0.2 121,331 + GFF5255 0.87 -1.0 121,332 - GFF5255 0.87 -0.2 121,332 - GFF5255 0.87 +0.2 121,332 - GFF5255 0.87 -0.3
Or see this region's nucleotide sequence