Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5251 and GFF5252 are separated by 184 nucleotides GFF5252 and GFF5253 are separated by 1 nucleotides
GFF5251 - Outer membrane porin protein 32 precursor, at 115,045 to 116,331
GFF5251
GFF5252 - ABC transporter, permease protein 1 (cluster 5, nickel/peptides/opines), at 116,516 to 117,496
GFF5252
GFF5253 - Amidohydrolase family protein, at 117,498 to 118,769
GFF5253
Position (kb)
116
117
118 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 115.619 kb on - strand, within GFF5251 at 116.011 kb on + strand, within GFF5251 at 116.330 kb on - strand at 116.346 kb on - strand at 116.346 kb on - strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.489 kb on - strand at 116.489 kb on - strand at 116.489 kb on - strand at 116.489 kb on - strand at 116.489 kb on - strand at 116.489 kb on - strand at 116.489 kb on - strand at 116.489 kb on - strand at 116.489 kb on - strand at 116.489 kb on - strand at 116.489 kb on - strand at 116.489 kb on - strand at 116.489 kb on - strand at 116.489 kb on - strand at 116.529 kb on + strand at 116.529 kb on + strand at 116.529 kb on + strand at 116.529 kb on + strand at 116.529 kb on + strand at 116.530 kb on - strand at 116.530 kb on - strand at 116.592 kb on + strand at 116.592 kb on + strand at 116.592 kb on + strand at 116.592 kb on + strand at 116.592 kb on + strand at 116.600 kb on + strand at 116.600 kb on + strand at 116.600 kb on + strand at 116.600 kb on + strand at 116.600 kb on + strand at 116.600 kb on + strand at 116.600 kb on + strand at 116.600 kb on + strand at 116.600 kb on + strand at 116.600 kb on + strand at 116.601 kb on - strand at 116.601 kb on - strand at 116.601 kb on - strand at 116.601 kb on - strand at 116.601 kb on - strand at 116.601 kb on - strand at 116.601 kb on - strand at 116.601 kb on - strand at 116.867 kb on + strand, within GFF5252 at 116.867 kb on + strand, within GFF5252 at 116.867 kb on + strand, within GFF5252 at 116.867 kb on + strand, within GFF5252 at 116.867 kb on + strand, within GFF5252 at 116.867 kb on + strand, within GFF5252 at 116.868 kb on - strand, within GFF5252 at 116.868 kb on - strand, within GFF5252 at 117.170 kb on + strand, within GFF5252 at 117.170 kb on + strand, within GFF5252 at 117.170 kb on + strand, within GFF5252 at 117.170 kb on + strand, within GFF5252 at 117.170 kb on + strand, within GFF5252 at 117.170 kb on + strand, within GFF5252 at 117.170 kb on + strand, within GFF5252 at 117.170 kb on + strand, within GFF5252 at 117.171 kb on - strand, within GFF5252 at 117.171 kb on - strand, within GFF5252 at 117.171 kb on - strand, within GFF5252 at 117.171 kb on - strand, within GFF5252 at 117.171 kb on - strand, within GFF5252 at 117.171 kb on - strand, within GFF5252 at 117.171 kb on - strand, within GFF5252 at 117.171 kb on - strand, within GFF5252 at 117.171 kb on - strand, within GFF5252 at 117.171 kb on - strand, within GFF5252 at 117.389 kb on + strand, within GFF5252 at 117.389 kb on + strand, within GFF5252 at 117.390 kb on - strand, within GFF5252 at 117.414 kb on - strand at 117.446 kb on + strand at 117.446 kb on + strand at 117.446 kb on + strand at 117.446 kb on + strand at 117.446 kb on + strand at 117.446 kb on + strand at 117.446 kb on + strand at 117.446 kb on + strand at 117.446 kb on + strand at 117.447 kb on - strand at 117.447 kb on - strand at 117.447 kb on - strand at 117.447 kb on - strand at 117.447 kb on - strand at 117.447 kb on - strand at 117.447 kb on - strand at 117.447 kb on - strand at 117.571 kb on - strand at 117.636 kb on + strand, within GFF5253 at 117.636 kb on + strand, within GFF5253 at 117.636 kb on + strand, within GFF5253 at 117.636 kb on + strand, within GFF5253 at 117.636 kb on + strand, within GFF5253 at 117.636 kb on + strand, within GFF5253 at 117.636 kb on + strand, within GFF5253 at 117.636 kb on + strand, within GFF5253 at 117.636 kb on + strand, within GFF5253 at 117.636 kb on + strand, within GFF5253 at 117.637 kb on - strand, within GFF5253 at 117.637 kb on - strand, within GFF5253 at 117.637 kb on - strand, within GFF5253 at 117.637 kb on - strand, within GFF5253 at 117.637 kb on - strand, within GFF5253 at 117.637 kb on - strand, within GFF5253 at 117.637 kb on - strand, within GFF5253 at 117.895 kb on - strand, within GFF5253 at 118.084 kb on - strand, within GFF5253 at 118.149 kb on + strand, within GFF5253 at 118.150 kb on - strand, within GFF5253 at 118.425 kb on + strand, within GFF5253 at 118.425 kb on + strand, within GFF5253 at 118.425 kb on + strand, within GFF5253 at 118.425 kb on + strand, within GFF5253 at 118.425 kb on + strand, within GFF5253 at 118.426 kb on - strand, within GFF5253 at 118.426 kb on - strand, within GFF5253 at 118.426 kb on - strand, within GFF5253 at 118.426 kb on - strand, within GFF5253 at 118.426 kb on - strand, within GFF5253 at 118.426 kb on - strand, within GFF5253 at 118.437 kb on + strand, within GFF5253 at 118.437 kb on + strand, within GFF5253 at 118.437 kb on + strand, within GFF5253 at 118.438 kb on - strand, within GFF5253 at 118.438 kb on - strand, within GFF5253
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 115,619 - GFF5251 0.45 -0.5 116,011 + GFF5251 0.75 -0.2 116,330 - +0.3 116,346 - +0.4 116,346 - -0.4 116,488 + -0.7 116,488 + -0.4 116,488 + -0.8 116,488 + -0.3 116,488 + +0.4 116,488 + +0.6 116,488 + +0.2 116,488 + -0.4 116,488 + -0.7 116,488 + -0.6 116,488 + -1.4 116,488 + +0.5 116,488 + +0.4 116,488 + -0.4 116,488 + -0.2 116,488 + +1.4 116,488 + -0.3 116,488 + -0.7 116,488 + -0.8 116,489 - -0.6 116,489 - -0.3 116,489 - -0.1 116,489 - +0.3 116,489 - +0.5 116,489 - +0.0 116,489 - -0.4 116,489 - -1.4 116,489 - +0.1 116,489 - +0.3 116,489 - +0.4 116,489 - +0.2 116,489 - -0.2 116,489 - +0.2 116,529 + +0.1 116,529 + +0.7 116,529 + +0.2 116,529 + -0.7 116,529 + +0.2 116,530 - +0.9 116,530 - -0.3 116,592 + +0.8 116,592 + -0.0 116,592 + +0.0 116,592 + -0.2 116,592 + +0.6 116,600 + -0.4 116,600 + +0.6 116,600 + -0.7 116,600 + +0.8 116,600 + -0.4 116,600 + +0.3 116,600 + -0.3 116,600 + +0.8 116,600 + +0.3 116,600 + -0.6 116,601 - -0.0 116,601 - -0.7 116,601 - +0.4 116,601 - -0.3 116,601 - -0.3 116,601 - +0.4 116,601 - -0.2 116,601 - -0.5 116,867 + GFF5252 0.36 -0.7 116,867 + GFF5252 0.36 -0.6 116,867 + GFF5252 0.36 -1.2 116,867 + GFF5252 0.36 -0.6 116,867 + GFF5252 0.36 -1.0 116,867 + GFF5252 0.36 +0.2 116,868 - GFF5252 0.36 -0.4 116,868 - GFF5252 0.36 -0.9 117,170 + GFF5252 0.67 +0.5 117,170 + GFF5252 0.67 +0.4 117,170 + GFF5252 0.67 -0.5 117,170 + GFF5252 0.67 -0.8 117,170 + GFF5252 0.67 -1.5 117,170 + GFF5252 0.67 -0.2 117,170 + GFF5252 0.67 -0.4 117,170 + GFF5252 0.67 -0.6 117,171 - GFF5252 0.67 -0.6 117,171 - GFF5252 0.67 +1.1 117,171 - GFF5252 0.67 -0.3 117,171 - GFF5252 0.67 -0.6 117,171 - GFF5252 0.67 -0.2 117,171 - GFF5252 0.67 -0.4 117,171 - GFF5252 0.67 -0.9 117,171 - GFF5252 0.67 -0.7 117,171 - GFF5252 0.67 -0.0 117,171 - GFF5252 0.67 -0.9 117,389 + GFF5252 0.89 -0.8 117,389 + GFF5252 0.89 +0.5 117,390 - GFF5252 0.89 -0.1 117,414 - -0.4 117,446 + +1.6 117,446 + -0.3 117,446 + -0.1 117,446 + -1.1 117,446 + -0.1 117,446 + +0.1 117,446 + +0.2 117,446 + +0.2 117,446 + -0.4 117,447 - -0.3 117,447 - +1.6 117,447 - +0.0 117,447 - -0.6 117,447 - -0.6 117,447 - +0.5 117,447 - +2.3 117,447 - -0.6 117,571 - -0.9 117,636 + GFF5253 0.11 +0.1 117,636 + GFF5253 0.11 -1.0 117,636 + GFF5253 0.11 +0.1 117,636 + GFF5253 0.11 -0.7 117,636 + GFF5253 0.11 -1.1 117,636 + GFF5253 0.11 +0.3 117,636 + GFF5253 0.11 -0.7 117,636 + GFF5253 0.11 +0.2 117,636 + GFF5253 0.11 -0.5 117,636 + GFF5253 0.11 -0.5 117,637 - GFF5253 0.11 -0.8 117,637 - GFF5253 0.11 -0.2 117,637 - GFF5253 0.11 -1.0 117,637 - GFF5253 0.11 -0.3 117,637 - GFF5253 0.11 +0.2 117,637 - GFF5253 0.11 -0.1 117,637 - GFF5253 0.11 -1.4 117,895 - GFF5253 0.31 +0.3 118,084 - GFF5253 0.46 -1.0 118,149 + GFF5253 0.51 +2.0 118,150 - GFF5253 0.51 -0.1 118,425 + GFF5253 0.73 -0.6 118,425 + GFF5253 0.73 -1.4 118,425 + GFF5253 0.73 -1.7 118,425 + GFF5253 0.73 -0.5 118,425 + GFF5253 0.73 -0.7 118,426 - GFF5253 0.73 -0.1 118,426 - GFF5253 0.73 +0.0 118,426 - GFF5253 0.73 -0.5 118,426 - GFF5253 0.73 +2.3 118,426 - GFF5253 0.73 -0.1 118,426 - GFF5253 0.73 +0.6 118,437 + GFF5253 0.74 +0.3 118,437 + GFF5253 0.74 -1.0 118,437 + GFF5253 0.74 +1.1 118,438 - GFF5253 0.74 +0.1 118,438 - GFF5253 0.74 -2.0
Or see this region's nucleotide sequence