Strain Fitness in Variovorax sp. SCN45 around GFF5229

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5228 and GFF5229 are separated by 176 nucleotidesGFF5229 and GFF5230 overlap by 14 nucleotides GFF5228 - Arachidonate 15-lipoxygenase precursor (EC 1.13.11.33), at 90,850 to 92,859 GFF5228 GFF5229 - Guanine deaminase (EC 3.5.4.3); Hydroxydechloroatrazine ethylaminohydrolase (EC 3.5.99.3), at 93,036 to 94,478 GFF5229 GFF5230 - Xanthine-uracil permease, at 94,465 to 95,880 GFF5230 Position (kb) 93 94 95Strain fitness (log2 ratio) -1 0 1at 92.051 kb on + strand, within GFF5228at 92.063 kb on + strand, within GFF5228at 92.124 kb on - strand, within GFF5228at 92.173 kb on + strand, within GFF5228at 92.208 kb on - strand, within GFF5228at 92.254 kb on - strand, within GFF5228at 92.681 kb on + strandat 92.681 kb on + strandat 92.681 kb on + strandat 92.681 kb on + strandat 92.681 kb on + strandat 92.682 kb on - strandat 92.934 kb on + strandat 93.067 kb on + strandat 93.068 kb on - strandat 93.068 kb on - strandat 94.027 kb on + strand, within GFF5229at 94.027 kb on + strand, within GFF5229at 94.028 kb on - strand, within GFF5229at 94.028 kb on - strand, within GFF5229at 94.133 kb on - strand, within GFF5229at 94.201 kb on + strand, within GFF5229at 94.201 kb on + strand, within GFF5229at 94.201 kb on + strand, within GFF5229at 94.202 kb on - strand, within GFF5229at 94.202 kb on - strand, within GFF5229at 94.347 kb on + strandat 94.347 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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92,051 + GFF5228 0.60 -0.4
92,063 + GFF5228 0.60 -0.9
92,124 - GFF5228 0.63 +0.0
92,173 + GFF5228 0.66 +1.2
92,208 - GFF5228 0.68 +0.5
92,254 - GFF5228 0.70 -0.6
92,681 + +0.0
92,681 + -0.7
92,681 + +0.3
92,681 + -0.1
92,681 + +0.6
92,682 - -0.8
92,934 + -0.1
93,067 + -1.7
93,068 - +0.9
93,068 - -0.8
94,027 + GFF5229 0.69 -0.7
94,027 + GFF5229 0.69 -0.6
94,028 - GFF5229 0.69 -0.2
94,028 - GFF5229 0.69 -0.4
94,133 - GFF5229 0.76 -1.5
94,201 + GFF5229 0.81 +0.3
94,201 + GFF5229 0.81 -0.5
94,201 + GFF5229 0.81 -1.2
94,202 - GFF5229 0.81 -0.7
94,202 - GFF5229 0.81 -0.2
94,347 + +0.6
94,347 + -0.1

Or see this region's nucleotide sequence