Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5200 and GFF5201 are separated by 9 nucleotides GFF5201 and GFF5202 are separated by 52 nucleotides GFF5202 and GFF5203 are separated by 165 nucleotides GFF5203 and GFF5204 are separated by 66 nucleotides
GFF5200 - L-arabonate dehydratase (EC 4.2.1.25), at 60,267 to 62,003
GFF5200
GFF5201 - L-2-keto-3-deoxyarabonate dehydratase (EC 4.2.1.43), at 62,013 to 62,930
GFF5201
GFF5202 - hypothetical protein, at 62,983 to 63,261
GFF5202
GFF5203 - hypothetical protein, at 63,427 to 63,858
GFF5203
GFF5204 - hypothetical protein, at 63,925 to 64,329
GFF5204
Position (kb)
62
63 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 61.218 kb on + strand, within GFF5200 at 61.219 kb on - strand, within GFF5200 at 61.512 kb on + strand, within GFF5200 at 61.512 kb on + strand, within GFF5200 at 61.512 kb on + strand, within GFF5200 at 61.512 kb on + strand, within GFF5200 at 61.593 kb on + strand, within GFF5200 at 61.701 kb on + strand, within GFF5200 at 61.706 kb on + strand, within GFF5200 at 61.706 kb on + strand, within GFF5200 at 61.911 kb on + strand at 61.911 kb on + strand at 61.911 kb on + strand at 62.026 kb on - strand at 62.223 kb on + strand, within GFF5201 at 62.260 kb on - strand, within GFF5201 at 62.385 kb on + strand, within GFF5201 at 62.385 kb on + strand, within GFF5201 at 62.385 kb on + strand, within GFF5201 at 62.385 kb on + strand, within GFF5201 at 62.386 kb on - strand, within GFF5201 at 62.386 kb on - strand, within GFF5201 at 62.506 kb on - strand, within GFF5201 at 62.506 kb on - strand, within GFF5201 at 62.515 kb on + strand, within GFF5201 at 62.516 kb on - strand, within GFF5201 at 62.731 kb on - strand, within GFF5201 at 62.731 kb on - strand, within GFF5201 at 62.757 kb on + strand, within GFF5201 at 62.758 kb on - strand, within GFF5201 at 63.068 kb on + strand, within GFF5202 at 63.068 kb on + strand, within GFF5202 at 63.068 kb on + strand, within GFF5202 at 63.068 kb on + strand, within GFF5202 at 63.069 kb on - strand, within GFF5202 at 63.072 kb on - strand, within GFF5202 at 63.125 kb on + strand, within GFF5202 at 63.125 kb on + strand, within GFF5202 at 63.125 kb on + strand, within GFF5202 at 63.126 kb on - strand, within GFF5202 at 63.137 kb on + strand, within GFF5202 at 63.137 kb on + strand, within GFF5202 at 63.138 kb on + strand, within GFF5202 at 63.138 kb on - strand, within GFF5202 at 63.230 kb on - strand, within GFF5202 at 63.230 kb on - strand, within GFF5202 at 63.230 kb on - strand, within GFF5202 at 63.230 kb on - strand, within GFF5202 at 63.280 kb on - strand at 63.297 kb on + strand at 63.297 kb on + strand at 63.297 kb on + strand at 63.297 kb on + strand at 63.297 kb on + strand at 63.297 kb on + strand at 63.297 kb on + strand at 63.297 kb on + strand at 63.297 kb on + strand at 63.297 kb on + strand at 63.298 kb on - strand at 63.298 kb on - strand at 63.298 kb on - strand at 63.298 kb on - strand at 63.298 kb on - strand at 63.298 kb on - strand at 63.298 kb on - strand at 63.298 kb on - strand at 63.298 kb on - strand at 63.299 kb on + strand at 63.299 kb on + strand at 63.299 kb on + strand at 63.299 kb on + strand at 63.299 kb on + strand at 63.299 kb on + strand at 63.299 kb on + strand at 63.300 kb on - strand at 63.300 kb on - strand at 63.300 kb on - strand at 63.300 kb on - strand at 63.300 kb on - strand at 63.300 kb on - strand at 63.300 kb on - strand at 63.303 kb on + strand at 63.303 kb on + strand at 63.303 kb on + strand at 63.303 kb on + strand at 63.303 kb on + strand at 63.303 kb on + strand at 63.303 kb on + strand at 63.303 kb on + strand at 63.303 kb on + strand at 63.303 kb on + strand at 63.304 kb on - strand at 63.304 kb on - strand at 63.304 kb on - strand at 63.304 kb on - strand at 63.304 kb on - strand at 63.304 kb on - strand at 63.304 kb on - strand at 63.304 kb on - strand at 63.304 kb on - strand at 63.321 kb on + strand at 63.347 kb on + strand at 63.347 kb on + strand at 63.758 kb on + strand, within GFF5203 at 63.758 kb on + strand, within GFF5203 at 63.846 kb on + strand at 63.846 kb on + strand at 63.847 kb on - strand at 63.847 kb on - strand at 63.847 kb on - strand at 63.880 kb on + strand at 63.880 kb on + strand at 63.881 kb on - strand at 63.906 kb on - strand at 63.906 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 61,218 + GFF5200 0.55 +0.6 61,219 - GFF5200 0.55 -1.3 61,512 + GFF5200 0.72 -0.2 61,512 + GFF5200 0.72 -0.7 61,512 + GFF5200 0.72 +0.3 61,512 + GFF5200 0.72 -0.3 61,593 + GFF5200 0.76 -0.5 61,701 + GFF5200 0.83 +1.1 61,706 + GFF5200 0.83 +0.3 61,706 + GFF5200 0.83 +0.8 61,911 + -0.1 61,911 + -1.4 61,911 + -0.4 62,026 - +1.3 62,223 + GFF5201 0.23 +0.2 62,260 - GFF5201 0.27 -0.7 62,385 + GFF5201 0.41 -0.4 62,385 + GFF5201 0.41 +1.1 62,385 + GFF5201 0.41 +0.6 62,385 + GFF5201 0.41 -0.5 62,386 - GFF5201 0.41 -2.0 62,386 - GFF5201 0.41 -0.5 62,506 - GFF5201 0.54 -0.1 62,506 - GFF5201 0.54 +0.6 62,515 + GFF5201 0.55 -1.8 62,516 - GFF5201 0.55 -0.8 62,731 - GFF5201 0.78 -0.6 62,731 - GFF5201 0.78 -0.3 62,757 + GFF5201 0.81 -1.6 62,758 - GFF5201 0.81 -0.0 63,068 + GFF5202 0.30 +1.2 63,068 + GFF5202 0.30 +0.7 63,068 + GFF5202 0.30 +0.4 63,068 + GFF5202 0.30 +1.3 63,069 - GFF5202 0.31 -0.7 63,072 - GFF5202 0.32 -0.0 63,125 + GFF5202 0.51 +1.0 63,125 + GFF5202 0.51 +0.4 63,125 + GFF5202 0.51 -0.1 63,126 - GFF5202 0.51 -0.6 63,137 + GFF5202 0.55 -0.4 63,137 + GFF5202 0.55 -1.4 63,138 + GFF5202 0.56 -0.2 63,138 - GFF5202 0.56 -0.3 63,230 - GFF5202 0.89 -0.2 63,230 - GFF5202 0.89 -0.1 63,230 - GFF5202 0.89 +0.4 63,230 - GFF5202 0.89 -0.3 63,280 - +0.1 63,297 + +0.5 63,297 + +0.2 63,297 + -1.9 63,297 + +1.2 63,297 + -0.0 63,297 + -0.7 63,297 + +0.3 63,297 + -1.1 63,297 + +0.8 63,297 + +1.3 63,298 - +1.5 63,298 - -0.7 63,298 - -0.4 63,298 - +0.6 63,298 - -0.2 63,298 - -1.3 63,298 - -0.0 63,298 - +0.9 63,298 - -0.2 63,299 + +1.3 63,299 + -0.9 63,299 + -0.2 63,299 + +0.3 63,299 + +2.1 63,299 + +0.1 63,299 + -1.0 63,300 - +0.0 63,300 - -0.2 63,300 - -0.4 63,300 - -0.5 63,300 - +0.3 63,300 - -1.5 63,300 - -0.5 63,303 + -0.0 63,303 + -0.7 63,303 + +0.2 63,303 + -0.4 63,303 + -0.6 63,303 + -0.5 63,303 + -0.2 63,303 + +0.6 63,303 + +1.0 63,303 + +1.2 63,304 - +0.0 63,304 - -0.7 63,304 - +1.0 63,304 - +0.7 63,304 - +0.4 63,304 - -0.7 63,304 - -0.4 63,304 - +1.0 63,304 - -0.8 63,321 + -0.6 63,347 + -0.2 63,347 + -0.7 63,758 + GFF5203 0.77 +0.0 63,758 + GFF5203 0.77 +0.3 63,846 + +0.7 63,846 + +0.4 63,847 - +0.0 63,847 - +0.5 63,847 - +1.1 63,880 + +1.0 63,880 + +0.1 63,881 - -1.0 63,906 - -0.4 63,906 - -0.2
Or see this region's nucleotide sequence