Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5096 and GFF5097 are separated by 13 nucleotides GFF5097 and GFF5098 are separated by 11 nucleotides GFF5098 and GFF5099 are separated by 11 nucleotides GFF5099 and GFF5100 are separated by 29 nucleotides
GFF5096 - similar to protocatechuate 4,5-dioxygenase beta chain, at 198,439 to 199,266
GFF5096
GFF5097 - 5-carboxymethyl-2-hydroxymuconate delta-isomerase (EC 5.3.3.10), at 199,280 to 199,687
GFF5097
GFF5098 - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-), at 199,699 to 200,502
GFF5098
GFF5099 - 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.52), at 200,514 to 201,335
GFF5099
GFF5100 - putative membrane protein, at 201,365 to 201,835
GFF5100
Position (kb)
199
200
201 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 198.758 kb on + strand, within GFF5096 at 198.758 kb on + strand, within GFF5096 at 199.120 kb on + strand, within GFF5096 at 199.120 kb on + strand, within GFF5096 at 199.121 kb on - strand, within GFF5096 at 199.301 kb on + strand at 199.302 kb on - strand at 199.430 kb on + strand, within GFF5097 at 199.445 kb on + strand, within GFF5097 at 199.485 kb on - strand, within GFF5097 at 199.640 kb on + strand, within GFF5097 at 199.640 kb on + strand, within GFF5097 at 199.641 kb on - strand, within GFF5097 at 199.816 kb on + strand, within GFF5098 at 199.937 kb on - strand, within GFF5098 at 200.095 kb on + strand, within GFF5098 at 200.096 kb on - strand, within GFF5098 at 200.276 kb on - strand, within GFF5098 at 200.276 kb on - strand, within GFF5098 at 200.276 kb on - strand, within GFF5098 at 200.276 kb on - strand, within GFF5098 at 200.598 kb on + strand, within GFF5099 at 200.790 kb on + strand, within GFF5099 at 200.790 kb on + strand, within GFF5099 at 200.790 kb on + strand, within GFF5099 at 200.791 kb on - strand, within GFF5099 at 200.946 kb on + strand, within GFF5099 at 200.946 kb on + strand, within GFF5099 at 200.946 kb on + strand, within GFF5099 at 200.946 kb on + strand, within GFF5099 at 200.946 kb on + strand, within GFF5099 at 200.946 kb on + strand, within GFF5099 at 200.947 kb on - strand, within GFF5099 at 200.947 kb on - strand, within GFF5099 at 200.947 kb on - strand, within GFF5099 at 201.294 kb on + strand at 201.294 kb on + strand at 201.294 kb on + strand at 201.294 kb on + strand at 201.294 kb on + strand at 201.294 kb on + strand at 201.295 kb on - strand at 201.330 kb on + strand at 201.330 kb on + strand at 201.331 kb on - strand at 201.331 kb on - strand at 201.497 kb on + strand, within GFF5100 at 201.497 kb on + strand, within GFF5100
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 198,758 + GFF5096 0.39 +0.2 198,758 + GFF5096 0.39 -0.3 199,120 + GFF5096 0.82 +1.2 199,120 + GFF5096 0.82 +0.1 199,121 - GFF5096 0.82 -0.7 199,301 + -0.7 199,302 - -0.2 199,430 + GFF5097 0.37 -0.4 199,445 + GFF5097 0.40 -0.0 199,485 - GFF5097 0.50 -0.6 199,640 + GFF5097 0.88 -0.4 199,640 + GFF5097 0.88 +1.0 199,641 - GFF5097 0.88 +0.0 199,816 + GFF5098 0.15 -0.7 199,937 - GFF5098 0.30 -0.3 200,095 + GFF5098 0.49 +0.4 200,096 - GFF5098 0.49 +0.6 200,276 - GFF5098 0.72 -0.2 200,276 - GFF5098 0.72 -0.6 200,276 - GFF5098 0.72 +0.8 200,276 - GFF5098 0.72 -0.7 200,598 + GFF5099 0.10 +0.4 200,790 + GFF5099 0.34 +1.8 200,790 + GFF5099 0.34 -0.0 200,790 + GFF5099 0.34 -0.5 200,791 - GFF5099 0.34 -0.8 200,946 + GFF5099 0.53 -0.8 200,946 + GFF5099 0.53 -1.8 200,946 + GFF5099 0.53 +0.3 200,946 + GFF5099 0.53 -0.1 200,946 + GFF5099 0.53 -0.5 200,946 + GFF5099 0.53 +0.2 200,947 - GFF5099 0.53 -1.0 200,947 - GFF5099 0.53 +0.5 200,947 - GFF5099 0.53 -0.5 201,294 + +0.0 201,294 + -0.1 201,294 + -0.4 201,294 + -0.1 201,294 + +0.6 201,294 + -0.7 201,295 - +0.9 201,330 + -0.3 201,330 + +1.3 201,331 - -0.8 201,331 - -0.2 201,497 + GFF5100 0.28 -0.3 201,497 + GFF5100 0.28 +0.2
Or see this region's nucleotide sequence