Strain Fitness in Variovorax sp. SCN45 around GFF5098

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5096 and GFF5097 are separated by 13 nucleotidesGFF5097 and GFF5098 are separated by 11 nucleotidesGFF5098 and GFF5099 are separated by 11 nucleotidesGFF5099 and GFF5100 are separated by 29 nucleotides GFF5096 - similar to protocatechuate 4,5-dioxygenase beta chain, at 198,439 to 199,266 GFF5096 GFF5097 - 5-carboxymethyl-2-hydroxymuconate delta-isomerase (EC 5.3.3.10), at 199,280 to 199,687 GFF5097 GFF5098 - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-), at 199,699 to 200,502 GFF5098 GFF5099 - 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.52), at 200,514 to 201,335 GFF5099 GFF5100 - putative membrane protein, at 201,365 to 201,835 GFF5100 Position (kb) 199 200 201Strain fitness (log2 ratio) -2 -1 0 1 2at 198.758 kb on + strand, within GFF5096at 198.758 kb on + strand, within GFF5096at 199.120 kb on + strand, within GFF5096at 199.120 kb on + strand, within GFF5096at 199.121 kb on - strand, within GFF5096at 199.301 kb on + strandat 199.302 kb on - strandat 199.430 kb on + strand, within GFF5097at 199.445 kb on + strand, within GFF5097at 199.485 kb on - strand, within GFF5097at 199.640 kb on + strand, within GFF5097at 199.640 kb on + strand, within GFF5097at 199.641 kb on - strand, within GFF5097at 199.816 kb on + strand, within GFF5098at 199.937 kb on - strand, within GFF5098at 200.095 kb on + strand, within GFF5098at 200.096 kb on - strand, within GFF5098at 200.276 kb on - strand, within GFF5098at 200.276 kb on - strand, within GFF5098at 200.276 kb on - strand, within GFF5098at 200.276 kb on - strand, within GFF5098at 200.598 kb on + strand, within GFF5099at 200.790 kb on + strand, within GFF5099at 200.790 kb on + strand, within GFF5099at 200.790 kb on + strand, within GFF5099at 200.791 kb on - strand, within GFF5099at 200.946 kb on + strand, within GFF5099at 200.946 kb on + strand, within GFF5099at 200.946 kb on + strand, within GFF5099at 200.946 kb on + strand, within GFF5099at 200.946 kb on + strand, within GFF5099at 200.946 kb on + strand, within GFF5099at 200.947 kb on - strand, within GFF5099at 200.947 kb on - strand, within GFF5099at 200.947 kb on - strand, within GFF5099at 201.294 kb on + strandat 201.294 kb on + strandat 201.294 kb on + strandat 201.294 kb on + strandat 201.294 kb on + strandat 201.294 kb on + strandat 201.295 kb on - strandat 201.330 kb on + strandat 201.330 kb on + strandat 201.331 kb on - strandat 201.331 kb on - strandat 201.497 kb on + strand, within GFF5100at 201.497 kb on + strand, within GFF5100

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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198,758 + GFF5096 0.39 +0.2
198,758 + GFF5096 0.39 -0.3
199,120 + GFF5096 0.82 +1.2
199,120 + GFF5096 0.82 +0.1
199,121 - GFF5096 0.82 -0.7
199,301 + -0.7
199,302 - -0.2
199,430 + GFF5097 0.37 -0.4
199,445 + GFF5097 0.40 -0.0
199,485 - GFF5097 0.50 -0.6
199,640 + GFF5097 0.88 -0.4
199,640 + GFF5097 0.88 +1.0
199,641 - GFF5097 0.88 +0.0
199,816 + GFF5098 0.15 -0.7
199,937 - GFF5098 0.30 -0.3
200,095 + GFF5098 0.49 +0.4
200,096 - GFF5098 0.49 +0.6
200,276 - GFF5098 0.72 -0.2
200,276 - GFF5098 0.72 -0.6
200,276 - GFF5098 0.72 +0.8
200,276 - GFF5098 0.72 -0.7
200,598 + GFF5099 0.10 +0.4
200,790 + GFF5099 0.34 +1.8
200,790 + GFF5099 0.34 -0.0
200,790 + GFF5099 0.34 -0.5
200,791 - GFF5099 0.34 -0.8
200,946 + GFF5099 0.53 -0.8
200,946 + GFF5099 0.53 -1.8
200,946 + GFF5099 0.53 +0.3
200,946 + GFF5099 0.53 -0.1
200,946 + GFF5099 0.53 -0.5
200,946 + GFF5099 0.53 +0.2
200,947 - GFF5099 0.53 -1.0
200,947 - GFF5099 0.53 +0.5
200,947 - GFF5099 0.53 -0.5
201,294 + +0.0
201,294 + -0.1
201,294 + -0.4
201,294 + -0.1
201,294 + +0.6
201,294 + -0.7
201,295 - +0.9
201,330 + -0.3
201,330 + +1.3
201,331 - -0.8
201,331 - -0.2
201,497 + GFF5100 0.28 -0.3
201,497 + GFF5100 0.28 +0.2

Or see this region's nucleotide sequence