Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5088 and GFF5089 are separated by 107 nucleotides GFF5089 and GFF5090 are separated by 145 nucleotides GFF5090 and GFF5091 overlap by 4 nucleotides GFF5091 and GFF5092 overlap by 4 nucleotides
GFF5088 - hypothetical protein, at 191,959 to 192,978
GFF5088
GFF5089 - Transcriptional regulator, MarR family, at 193,086 to 193,592
GFF5089
GFF5090 - Homoprotocatechuate degradative operon repressor, at 193,738 to 194,175
GFF5090
GFF5091 - 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68), at 194,172 to 194,804
GFF5091
GFF5092 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC 5.3.3.-), at 194,801 to 195,583
GFF5092
Position (kb) 193
194
195 Strain fitness (log2 ratio) -2
-1
0
1
2 at 192.954 kb on - strand at 192.954 kb on - strand at 193.036 kb on - strand at 193.036 kb on - strand at 193.036 kb on - strand at 193.036 kb on - strand at 193.036 kb on - strand at 193.036 kb on - strand at 193.036 kb on - strand at 193.036 kb on - strand at 193.085 kb on + strand at 193.085 kb on + strand at 193.085 kb on + strand at 193.086 kb on - strand at 193.086 kb on - strand at 193.086 kb on - strand at 193.093 kb on + strand at 193.094 kb on - strand at 193.094 kb on - strand at 193.094 kb on - strand at 193.094 kb on - strand at 193.125 kb on + strand at 193.126 kb on - strand at 193.649 kb on - strand at 193.649 kb on - strand at 193.649 kb on - strand at 193.649 kb on - strand at 193.649 kb on - strand at 193.649 kb on - strand at 193.650 kb on - strand at 193.653 kb on + strand at 193.653 kb on + strand at 193.653 kb on + strand at 193.653 kb on + strand at 193.682 kb on + strand at 193.682 kb on + strand at 193.683 kb on - strand at 194.081 kb on - strand, within GFF5090 at 194.081 kb on - strand, within GFF5090 at 194.129 kb on - strand, within GFF5090 at 194.177 kb on + strand at 194.177 kb on + strand at 194.177 kb on + strand at 194.177 kb on + strand at 194.177 kb on + strand at 194.177 kb on + strand at 194.177 kb on + strand at 194.177 kb on + strand at 194.178 kb on - strand at 194.178 kb on - strand at 194.178 kb on - strand at 194.178 kb on - strand at 194.178 kb on - strand at 194.208 kb on + strand at 194.208 kb on + strand at 194.208 kb on + strand at 194.209 kb on - strand at 194.209 kb on - strand at 194.209 kb on - strand at 194.274 kb on + strand, within GFF5091 at 194.275 kb on - strand, within GFF5091 at 194.822 kb on + strand at 194.822 kb on + strand at 194.822 kb on + strand at 194.823 kb on - strand at 194.823 kb on - strand at 194.823 kb on - strand at 195.116 kb on + strand, within GFF5092 at 195.117 kb on - strand, within GFF5092 at 195.117 kb on - strand, within GFF5092 at 195.117 kb on - strand, within GFF5092
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 192,954 - -0.7 192,954 - +0.2 193,036 - -0.9 193,036 - -1.0 193,036 - -0.0 193,036 - -0.6 193,036 - -1.6 193,036 - +0.3 193,036 - -0.4 193,036 - +0.6 193,085 + -1.9 193,085 + +0.3 193,085 + +0.4 193,086 - +0.4 193,086 - -0.8 193,086 - -0.2 193,093 + -0.2 193,094 - -0.3 193,094 - -0.8 193,094 - +0.4 193,094 - +0.2 193,125 + -1.4 193,126 - -0.1 193,649 - +0.3 193,649 - -0.4 193,649 - +0.5 193,649 - -0.4 193,649 - -0.8 193,649 - -0.7 193,650 - -1.6 193,653 + +1.0 193,653 + -0.2 193,653 + +0.8 193,653 + +0.3 193,682 + -0.4 193,682 + +0.8 193,683 - +0.3 194,081 - GFF5090 0.78 -0.7 194,081 - GFF5090 0.78 -1.0 194,129 - GFF5090 0.89 -0.6 194,177 + +0.2 194,177 + -0.1 194,177 + -0.4 194,177 + -0.6 194,177 + -0.6 194,177 + +1.0 194,177 + -0.1 194,177 + -0.6 194,178 - -0.0 194,178 - +0.3 194,178 - -0.5 194,178 - +0.3 194,178 - +0.2 194,208 + -0.8 194,208 + +1.8 194,208 + -0.1 194,209 - -0.0 194,209 - -0.6 194,209 - +1.5 194,274 + GFF5091 0.16 -0.0 194,275 - GFF5091 0.16 -0.3 194,822 + -1.6 194,822 + -0.4 194,822 + -1.5 194,823 - +0.2 194,823 - +2.2 194,823 - -0.9 195,116 + GFF5092 0.40 +0.0 195,117 - GFF5092 0.40 -0.7 195,117 - GFF5092 0.40 +2.2 195,117 - GFF5092 0.40 +0.7
Or see this region's nucleotide sequence