Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4957 and GFF4958 are separated by 130 nucleotides GFF4958 and GFF4959 overlap by 4 nucleotides GFF4959 and GFF4960 overlap by 4 nucleotides
GFF4957 - ABC transporter, substrate-binding protein (cluster 4, leucine/isoleucine/valine/benzoate), at 57,550 to 58,755
GFF4957
GFF4958 - ABC transporter, permease protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 58,886 to 59,806
GFF4958
GFF4959 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 59,803 to 60,792
GFF4959
GFF4960 - ABC transporter, ATP-binding protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 60,789 to 61,544
GFF4960
Position (kb)
58
59
60 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 57.932 kb on - strand, within GFF4957 at 57.986 kb on - strand, within GFF4957 at 58.156 kb on + strand, within GFF4957 at 58.157 kb on - strand, within GFF4957 at 58.157 kb on - strand, within GFF4957 at 58.228 kb on + strand, within GFF4957 at 58.228 kb on + strand, within GFF4957 at 58.228 kb on + strand, within GFF4957 at 58.474 kb on + strand, within GFF4957 at 58.489 kb on + strand, within GFF4957 at 58.585 kb on + strand, within GFF4957 at 58.586 kb on - strand, within GFF4957 at 58.586 kb on - strand, within GFF4957 at 58.586 kb on - strand, within GFF4957 at 58.627 kb on + strand, within GFF4957 at 58.645 kb on + strand at 58.729 kb on + strand at 58.730 kb on - strand at 58.730 kb on - strand at 58.730 kb on - strand at 58.730 kb on - strand at 58.885 kb on + strand at 58.886 kb on - strand at 58.886 kb on - strand at 58.886 kb on - strand at 59.361 kb on - strand, within GFF4958 at 59.556 kb on + strand, within GFF4958 at 59.557 kb on - strand, within GFF4958 at 59.557 kb on - strand, within GFF4958 at 59.557 kb on - strand, within GFF4958 at 59.744 kb on + strand at 59.745 kb on - strand at 59.745 kb on - strand at 59.745 kb on - strand at 59.745 kb on - strand at 59.824 kb on + strand at 59.824 kb on + strand at 59.825 kb on - strand at 59.899 kb on + strand at 59.899 kb on + strand at 59.899 kb on + strand at 60.019 kb on + strand, within GFF4959 at 60.145 kb on + strand, within GFF4959 at 60.146 kb on - strand, within GFF4959 at 60.184 kb on + strand, within GFF4959 at 60.185 kb on - strand, within GFF4959 at 60.230 kb on - strand, within GFF4959
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 57,932 - GFF4957 0.32 -0.5 57,986 - GFF4957 0.36 -0.6 58,156 + GFF4957 0.50 -0.6 58,157 - GFF4957 0.50 +0.2 58,157 - GFF4957 0.50 -0.6 58,228 + GFF4957 0.56 +0.4 58,228 + GFF4957 0.56 +0.1 58,228 + GFF4957 0.56 +0.6 58,474 + GFF4957 0.77 +0.8 58,489 + GFF4957 0.78 +0.4 58,585 + GFF4957 0.86 +0.5 58,586 - GFF4957 0.86 -0.9 58,586 - GFF4957 0.86 -0.2 58,586 - GFF4957 0.86 -0.7 58,627 + GFF4957 0.89 -0.3 58,645 + -0.2 58,729 + -0.2 58,730 - -0.4 58,730 - +0.8 58,730 - +0.0 58,730 - -0.7 58,885 + -0.0 58,886 - +0.3 58,886 - -0.9 58,886 - +0.7 59,361 - GFF4958 0.52 +0.1 59,556 + GFF4958 0.73 -0.1 59,557 - GFF4958 0.73 -0.1 59,557 - GFF4958 0.73 +0.5 59,557 - GFF4958 0.73 +0.5 59,744 + -0.5 59,745 - +1.4 59,745 - -0.3 59,745 - -0.2 59,745 - -0.5 59,824 + -1.6 59,824 + -0.9 59,825 - -0.7 59,899 + +0.4 59,899 + +2.3 59,899 + +0.4 60,019 + GFF4959 0.22 -0.5 60,145 + GFF4959 0.35 -1.9 60,146 - GFF4959 0.35 -0.6 60,184 + GFF4959 0.38 -0.4 60,185 - GFF4959 0.39 -0.1 60,230 - GFF4959 0.43 +0.0
Or see this region's nucleotide sequence