Strain Fitness in Variovorax sp. SCN45 around GFF460

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF459 and GFF460 overlap by 4 nucleotidesGFF460 and GFF461 are separated by 15 nucleotidesGFF461 and GFF462 overlap by 4 nucleotides GFF459 - Beta-lactamase class C-like and penicillin binding proteins (PBPs) superfamily / DUF3471 domain, at 497,159 to 498,604 GFF459 GFF460 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 498,601 to 500,139 GFF460 GFF461 - Enoyl-CoA hydratase (EC 4.2.1.17), at 500,155 to 500,955 GFF461 GFF462 - BUG/TctC family periplasmic protein, at 500,952 to 501,938 GFF462 Position (kb) 498 499 500 501Strain fitness (log2 ratio) -2 -1 0 1 2at 497.618 kb on + strand, within GFF459at 497.618 kb on + strand, within GFF459at 497.618 kb on + strand, within GFF459at 497.618 kb on + strand, within GFF459at 497.618 kb on + strand, within GFF459at 497.619 kb on - strand, within GFF459at 497.619 kb on - strand, within GFF459at 498.041 kb on + strand, within GFF459at 498.041 kb on + strand, within GFF459at 498.042 kb on - strand, within GFF459at 498.161 kb on + strand, within GFF459at 498.161 kb on + strand, within GFF459at 498.162 kb on - strand, within GFF459at 498.320 kb on + strand, within GFF459at 498.320 kb on - strand, within GFF459at 498.321 kb on - strand, within GFF459at 498.321 kb on - strand, within GFF459at 498.333 kb on - strand, within GFF459at 498.333 kb on - strand, within GFF459at 498.333 kb on - strand, within GFF459at 498.348 kb on - strand, within GFF459at 498.431 kb on + strand, within GFF459at 498.431 kb on + strand, within GFF459at 498.431 kb on + strand, within GFF459at 498.501 kb on - strandat 498.866 kb on + strand, within GFF460at 498.867 kb on - strand, within GFF460at 498.887 kb on + strand, within GFF460at 498.896 kb on + strand, within GFF460at 498.896 kb on + strand, within GFF460at 498.897 kb on - strand, within GFF460at 498.897 kb on - strand, within GFF460at 498.897 kb on - strand, within GFF460at 498.897 kb on - strand, within GFF460at 498.897 kb on - strand, within GFF460at 498.944 kb on + strand, within GFF460at 498.944 kb on + strand, within GFF460at 498.944 kb on + strand, within GFF460at 498.944 kb on + strand, within GFF460at 499.034 kb on + strand, within GFF460at 499.034 kb on + strand, within GFF460at 499.035 kb on - strand, within GFF460at 499.184 kb on + strand, within GFF460at 499.184 kb on + strand, within GFF460at 499.184 kb on + strand, within GFF460at 499.185 kb on - strand, within GFF460at 499.185 kb on - strand, within GFF460at 499.185 kb on - strand, within GFF460at 499.185 kb on - strand, within GFF460at 499.185 kb on - strand, within GFF460at 499.364 kb on + strand, within GFF460at 499.364 kb on + strand, within GFF460at 499.364 kb on + strand, within GFF460at 499.364 kb on + strand, within GFF460at 499.365 kb on - strand, within GFF460at 499.365 kb on - strand, within GFF460at 499.365 kb on - strand, within GFF460at 499.365 kb on - strand, within GFF460at 499.365 kb on - strand, within GFF460at 499.553 kb on + strand, within GFF460at 499.553 kb on + strand, within GFF460at 499.554 kb on - strand, within GFF460at 499.554 kb on - strand, within GFF460at 499.637 kb on + strand, within GFF460at 499.638 kb on - strand, within GFF460at 499.806 kb on + strand, within GFF460at 499.806 kb on + strand, within GFF460at 499.806 kb on + strand, within GFF460at 499.807 kb on - strand, within GFF460at 499.807 kb on - strand, within GFF460at 499.841 kb on + strand, within GFF460at 499.845 kb on - strand, within GFF460at 499.892 kb on + strand, within GFF460at 499.916 kb on + strand, within GFF460at 499.916 kb on + strand, within GFF460at 499.916 kb on + strand, within GFF460at 499.917 kb on - strand, within GFF460at 499.917 kb on - strand, within GFF460at 500.039 kb on + strandat 500.040 kb on - strandat 500.148 kb on + strandat 500.148 kb on + strandat 500.148 kb on + strandat 500.149 kb on - strandat 500.149 kb on - strandat 500.151 kb on + strandat 500.151 kb on + strandat 500.151 kb on + strandat 500.151 kb on + strandat 500.151 kb on + strandat 500.151 kb on + strandat 500.152 kb on - strandat 500.152 kb on - strandat 500.152 kb on - strandat 500.152 kb on - strandat 500.152 kb on - strandat 500.222 kb on + strandat 500.222 kb on + strandat 500.222 kb on + strandat 500.222 kb on + strandat 500.223 kb on - strandat 500.223 kb on - strandat 500.223 kb on - strandat 500.223 kb on - strandat 500.223 kb on - strandat 500.223 kb on - strandat 500.228 kb on + strandat 500.228 kb on + strandat 500.229 kb on - strandat 500.402 kb on + strand, within GFF461at 500.402 kb on + strand, within GFF461at 500.523 kb on + strand, within GFF461at 500.524 kb on - strand, within GFF461

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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497,618 + GFF459 0.32 -0.7
497,618 + GFF459 0.32 -1.5
497,618 + GFF459 0.32 -0.8
497,618 + GFF459 0.32 -0.4
497,618 + GFF459 0.32 -0.4
497,619 - GFF459 0.32 +0.2
497,619 - GFF459 0.32 -1.1
498,041 + GFF459 0.61 -0.0
498,041 + GFF459 0.61 -0.9
498,042 - GFF459 0.61 -0.4
498,161 + GFF459 0.69 -0.2
498,161 + GFF459 0.69 -1.5
498,162 - GFF459 0.69 +1.2
498,320 + GFF459 0.80 +0.0
498,320 - GFF459 0.80 -0.4
498,321 - GFF459 0.80 -0.3
498,321 - GFF459 0.80 +1.2
498,333 - GFF459 0.81 -0.1
498,333 - GFF459 0.81 -1.4
498,333 - GFF459 0.81 -0.8
498,348 - GFF459 0.82 -0.8
498,431 + GFF459 0.88 +0.4
498,431 + GFF459 0.88 -2.0
498,431 + GFF459 0.88 -0.4
498,501 - -0.0
498,866 + GFF460 0.17 -0.4
498,867 - GFF460 0.17 +0.2
498,887 + GFF460 0.19 +0.4
498,896 + GFF460 0.19 +0.2
498,896 + GFF460 0.19 -1.8
498,897 - GFF460 0.19 -1.2
498,897 - GFF460 0.19 -0.1
498,897 - GFF460 0.19 +0.0
498,897 - GFF460 0.19 +0.5
498,897 - GFF460 0.19 +0.8
498,944 + GFF460 0.22 +0.6
498,944 + GFF460 0.22 +0.6
498,944 + GFF460 0.22 +0.0
498,944 + GFF460 0.22 -0.5
499,034 + GFF460 0.28 +0.9
499,034 + GFF460 0.28 -0.1
499,035 - GFF460 0.28 +0.3
499,184 + GFF460 0.38 +1.0
499,184 + GFF460 0.38 +0.3
499,184 + GFF460 0.38 -0.2
499,185 - GFF460 0.38 -0.2
499,185 - GFF460 0.38 -0.8
499,185 - GFF460 0.38 -0.4
499,185 - GFF460 0.38 +0.1
499,185 - GFF460 0.38 -0.1
499,364 + GFF460 0.50 +0.2
499,364 + GFF460 0.50 +0.5
499,364 + GFF460 0.50 -0.6
499,364 + GFF460 0.50 -1.1
499,365 - GFF460 0.50 -0.7
499,365 - GFF460 0.50 +0.1
499,365 - GFF460 0.50 +0.1
499,365 - GFF460 0.50 -0.1
499,365 - GFF460 0.50 +0.0
499,553 + GFF460 0.62 +0.6
499,553 + GFF460 0.62 +0.1
499,554 - GFF460 0.62 +0.2
499,554 - GFF460 0.62 +0.3
499,637 + GFF460 0.67 +1.2
499,638 - GFF460 0.67 +0.5
499,806 + GFF460 0.78 +0.6
499,806 + GFF460 0.78 +1.8
499,806 + GFF460 0.78 -0.4
499,807 - GFF460 0.78 +0.3
499,807 - GFF460 0.78 +1.8
499,841 + GFF460 0.81 -2.3
499,845 - GFF460 0.81 -0.8
499,892 + GFF460 0.84 +0.8
499,916 + GFF460 0.85 +0.4
499,916 + GFF460 0.85 -0.8
499,916 + GFF460 0.85 -0.4
499,917 - GFF460 0.86 +0.5
499,917 - GFF460 0.86 -0.6
500,039 + -0.5
500,040 - +0.6
500,148 + -0.3
500,148 + -0.8
500,148 + -0.0
500,149 - -0.7
500,149 - -0.3
500,151 + +0.1
500,151 + +0.2
500,151 + +0.2
500,151 + +0.2
500,151 + +0.0
500,151 + -0.3
500,152 - +0.2
500,152 - +1.0
500,152 - -0.4
500,152 - -0.8
500,152 - +1.2
500,222 + -0.0
500,222 + +0.3
500,222 + -0.2
500,222 + +0.1
500,223 - +1.0
500,223 - -1.1
500,223 - -0.4
500,223 - -0.6
500,223 - -0.2
500,223 - +0.1
500,228 + -0.8
500,228 + -0.2
500,229 - -1.8
500,402 + GFF461 0.31 +0.9
500,402 + GFF461 0.31 -0.6
500,523 + GFF461 0.46 -0.6
500,524 - GFF461 0.46 -0.7

Or see this region's nucleotide sequence