Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4117 and GFF4118 are separated by 51 nucleotides GFF4118 and GFF4119 are separated by 141 nucleotides GFF4119 and GFF4120 are separated by 98 nucleotides GFF4120 and GFF4121 are separated by 605 nucleotides
GFF4117 - Translation elongation factor Tu, at 21,552 to 22,748
GFF4117
GFF4118 - no description, at 22,800 to 22,991
GFF4118
GFF4119 - no description, at 23,133 to 23,312
GFF4119
GFF4120 - no description, at 23,411 to 23,950
GFF4120
GFF4121 - no description, at 24,556 to 24,966
GFF4121
Position (kb) 23
24 Strain fitness (log2 ratio) -2
-1
0
1
2 at 22.501 kb on + strand, within GFF4117 at 22.535 kb on - strand, within GFF4117 at 22.624 kb on + strand, within GFF4117 at 23.134 kb on + strand at 23.134 kb on + strand at 23.288 kb on + strand, within GFF4119 at 23.288 kb on + strand, within GFF4119 at 23.633 kb on - strand, within GFF4120 at 23.786 kb on + strand, within GFF4120 at 23.866 kb on - strand, within GFF4120 at 23.949 kb on - strand at 24.175 kb on + strand at 24.176 kb on - strand at 24.187 kb on - strand at 24.215 kb on + strand at 24.216 kb on - strand at 24.235 kb on - strand at 24.254 kb on - strand at 24.303 kb on + strand at 24.304 kb on - strand at 24.316 kb on + strand at 24.320 kb on + strand at 24.352 kb on + strand at 24.436 kb on - strand at 24.561 kb on + strand at 24.562 kb on - strand at 24.603 kb on + strand, within GFF4121 at 24.725 kb on - strand, within GFF4121 at 24.783 kb on + strand, within GFF4121 at 24.783 kb on + strand, within GFF4121 at 24.783 kb on + strand, within GFF4121 at 24.784 kb on - strand, within GFF4121 at 24.882 kb on - strand, within GFF4121 at 24.893 kb on + strand, within GFF4121 at 24.894 kb on - strand, within GFF4121
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 22,501 + GFF4117 0.79 -0.3 22,535 - GFF4117 0.82 +0.0 22,624 + GFF4117 0.90 -0.7 23,134 + +0.6 23,134 + -0.2 23,288 + GFF4119 0.86 -0.7 23,288 + GFF4119 0.86 -0.7 23,633 - GFF4120 0.41 +0.6 23,786 + GFF4120 0.69 -0.9 23,866 - GFF4120 0.84 +0.3 23,949 - +0.2 24,175 + +0.3 24,176 - -2.4 24,187 - +0.5 24,215 + +0.0 24,216 - +0.1 24,235 - -0.8 24,254 - -0.7 24,303 + -1.7 24,304 - +0.5 24,316 + -0.1 24,320 + -0.1 24,352 + +0.0 24,436 - -0.1 24,561 + -1.1 24,562 - -0.1 24,603 + GFF4121 0.11 -2.1 24,725 - GFF4121 0.41 -0.4 24,783 + GFF4121 0.55 +1.9 24,783 + GFF4121 0.55 +0.4 24,783 + GFF4121 0.55 -0.3 24,784 - GFF4121 0.55 -0.7 24,882 - GFF4121 0.79 +0.0 24,893 + GFF4121 0.82 -0.3 24,894 - GFF4121 0.82 +0.0
Or see this region's nucleotide sequence