Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3985 and GFF3986 are separated by 4 nucleotides GFF3986 and GFF3987 are separated by 53 nucleotides GFF3987 and GFF3988 overlap by 4 nucleotides
GFF3985 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 189,107 to 190,696
GFF3985
GFF3986 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 190,701 to 191,447
GFF3986
GFF3987 - 3-hydroxybutyrate dehydrogenase (EC 1.1.1.30), at 191,501 to 192,277
GFF3987
GFF3988 - Transcriptional regulator, MarR family, at 192,274 to 192,804
GFF3988
Position (kb)
190
191
192 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 189.702 kb on - strand, within GFF3985 at 189.801 kb on - strand, within GFF3985 at 189.905 kb on + strand, within GFF3985 at 189.905 kb on + strand, within GFF3985 at 189.906 kb on - strand, within GFF3985 at 189.906 kb on - strand, within GFF3985 at 189.906 kb on - strand, within GFF3985 at 190.226 kb on + strand, within GFF3985 at 190.226 kb on + strand, within GFF3985 at 190.227 kb on - strand, within GFF3985 at 190.227 kb on - strand, within GFF3985 at 190.448 kb on + strand, within GFF3985 at 190.562 kb on + strand at 190.694 kb on + strand at 190.694 kb on + strand at 190.695 kb on - strand at 190.695 kb on - strand at 190.695 kb on - strand at 191.097 kb on - strand, within GFF3986 at 191.163 kb on + strand, within GFF3986 at 191.164 kb on - strand, within GFF3986 at 191.164 kb on - strand, within GFF3986 at 191.373 kb on + strand at 191.504 kb on + strand at 191.505 kb on - strand at 191.709 kb on + strand, within GFF3987 at 191.709 kb on + strand, within GFF3987 at 191.710 kb on - strand, within GFF3987 at 191.917 kb on + strand, within GFF3987 at 191.946 kb on - strand, within GFF3987 at 191.946 kb on - strand, within GFF3987 at 191.946 kb on - strand, within GFF3987 at 191.946 kb on - strand, within GFF3987 at 191.946 kb on - strand, within GFF3987 at 191.951 kb on + strand, within GFF3987 at 191.951 kb on + strand, within GFF3987 at 191.952 kb on - strand, within GFF3987 at 191.952 kb on - strand, within GFF3987 at 191.952 kb on - strand, within GFF3987 at 191.952 kb on - strand, within GFF3987 at 191.952 kb on - strand, within GFF3987 at 191.952 kb on - strand, within GFF3987 at 191.963 kb on + strand, within GFF3987 at 191.964 kb on - strand, within GFF3987 at 192.059 kb on + strand, within GFF3987 at 192.061 kb on + strand, within GFF3987 at 192.062 kb on - strand, within GFF3987 at 192.393 kb on + strand, within GFF3988 at 192.445 kb on - strand, within GFF3988
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 189,702 - GFF3985 0.37 +0.3 189,801 - GFF3985 0.44 -0.4 189,905 + GFF3985 0.50 +0.1 189,905 + GFF3985 0.50 -1.1 189,906 - GFF3985 0.50 +0.0 189,906 - GFF3985 0.50 -0.7 189,906 - GFF3985 0.50 -0.5 190,226 + GFF3985 0.70 +0.0 190,226 + GFF3985 0.70 +1.0 190,227 - GFF3985 0.70 -0.4 190,227 - GFF3985 0.70 -0.8 190,448 + GFF3985 0.84 -1.0 190,562 + -0.6 190,694 + -0.3 190,694 + +1.0 190,695 - +0.2 190,695 - +1.8 190,695 - +1.1 191,097 - GFF3986 0.53 -0.0 191,163 + GFF3986 0.62 +1.0 191,164 - GFF3986 0.62 +0.7 191,164 - GFF3986 0.62 -0.0 191,373 + -0.3 191,504 + -0.5 191,505 - -0.3 191,709 + GFF3987 0.27 -0.1 191,709 + GFF3987 0.27 -1.1 191,710 - GFF3987 0.27 -0.5 191,917 + GFF3987 0.54 +1.5 191,946 - GFF3987 0.57 -0.4 191,946 - GFF3987 0.57 -0.8 191,946 - GFF3987 0.57 +0.0 191,946 - GFF3987 0.57 +0.6 191,946 - GFF3987 0.57 -2.2 191,951 + GFF3987 0.58 -1.7 191,951 + GFF3987 0.58 +0.6 191,952 - GFF3987 0.58 -2.5 191,952 - GFF3987 0.58 +0.4 191,952 - GFF3987 0.58 -0.2 191,952 - GFF3987 0.58 +0.5 191,952 - GFF3987 0.58 +0.7 191,952 - GFF3987 0.58 +0.3 191,963 + GFF3987 0.59 +0.3 191,964 - GFF3987 0.60 -0.2 192,059 + GFF3987 0.72 -1.2 192,061 + GFF3987 0.72 +0.5 192,062 - GFF3987 0.72 +0.3 192,393 + GFF3988 0.22 -0.7 192,445 - GFF3988 0.32 +0.1
Or see this region's nucleotide sequence