Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3862 and GFF3863 are separated by 10 nucleotides GFF3863 and GFF3864 are separated by 17 nucleotides GFF3864 and GFF3865 are separated by 174 nucleotides
GFF3862 - hypothetical protein, at 60,210 to 60,692
GFF3862
GFF3863 - RNA polymerase sigma factor RpoE, at 60,703 to 61,380
GFF3863
GFF3864 - Sigma factor RpoE negative regulatory protein RseA, at 61,398 to 62,075
GFF3864
GFF3865 - Sigma factor RpoE negative regulatory protein RseB precursor, at 62,250 to 63,191
GFF3865
Position (kb) 61
62
63 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 60.430 kb on + strand, within GFF3862 at 60.619 kb on + strand, within GFF3862 at 60.619 kb on + strand, within GFF3862 at 60.619 kb on + strand, within GFF3862 at 60.619 kb on + strand, within GFF3862 at 60.619 kb on + strand, within GFF3862 at 60.619 kb on + strand, within GFF3862 at 60.619 kb on + strand, within GFF3862 at 60.619 kb on + strand, within GFF3862 at 60.619 kb on + strand, within GFF3862 at 60.620 kb on - strand, within GFF3862 at 60.620 kb on - strand, within GFF3862 at 60.620 kb on - strand, within GFF3862 at 60.620 kb on - strand, within GFF3862 at 60.620 kb on - strand, within GFF3862 at 60.621 kb on + strand, within GFF3862 at 60.621 kb on + strand, within GFF3862 at 60.621 kb on + strand, within GFF3862 at 60.621 kb on + strand, within GFF3862 at 60.621 kb on + strand, within GFF3862 at 60.621 kb on + strand, within GFF3862 at 60.622 kb on - strand, within GFF3862 at 60.622 kb on - strand, within GFF3862 at 60.622 kb on - strand, within GFF3862 at 60.622 kb on - strand, within GFF3862 at 60.622 kb on - strand, within GFF3862 at 60.622 kb on - strand, within GFF3862 at 60.622 kb on - strand, within GFF3862 at 60.622 kb on - strand, within GFF3862 at 60.622 kb on - strand, within GFF3862 at 60.622 kb on - strand, within GFF3862 at 60.622 kb on - strand, within GFF3862 at 60.622 kb on - strand, within GFF3862 at 60.622 kb on - strand, within GFF3862 at 60.622 kb on - strand, within GFF3862 at 60.622 kb on - strand, within GFF3862 at 60.622 kb on - strand, within GFF3862 at 60.622 kb on - strand, within GFF3862 at 60.622 kb on - strand, within GFF3862 at 60.622 kb on - strand, within GFF3862 at 60.622 kb on - strand, within GFF3862 at 60.622 kb on - strand, within GFF3862 at 60.622 kb on - strand, within GFF3862 at 60.709 kb on + strand at 60.853 kb on + strand, within GFF3863 at 60.853 kb on + strand, within GFF3863 at 60.853 kb on + strand, within GFF3863 at 60.853 kb on + strand, within GFF3863 at 60.853 kb on + strand, within GFF3863 at 60.853 kb on + strand, within GFF3863 at 60.854 kb on - strand, within GFF3863 at 60.854 kb on - strand, within GFF3863 at 60.854 kb on - strand, within GFF3863 at 60.854 kb on - strand, within GFF3863 at 60.854 kb on - strand, within GFF3863 at 60.854 kb on - strand, within GFF3863 at 60.854 kb on - strand, within GFF3863 at 60.854 kb on - strand, within GFF3863 at 60.916 kb on - strand, within GFF3863 at 60.982 kb on + strand, within GFF3863 at 60.982 kb on + strand, within GFF3863 at 60.982 kb on + strand, within GFF3863 at 60.982 kb on + strand, within GFF3863 at 60.982 kb on + strand, within GFF3863 at 60.982 kb on + strand, within GFF3863 at 60.982 kb on + strand, within GFF3863 at 60.982 kb on + strand, within GFF3863 at 60.982 kb on + strand, within GFF3863 at 60.982 kb on + strand, within GFF3863 at 60.982 kb on + strand, within GFF3863 at 60.982 kb on + strand, within GFF3863 at 60.982 kb on + strand, within GFF3863 at 60.982 kb on + strand, within GFF3863 at 60.983 kb on - strand, within GFF3863 at 60.983 kb on - strand, within GFF3863 at 60.983 kb on - strand, within GFF3863 at 60.983 kb on - strand, within GFF3863 at 60.983 kb on - strand, within GFF3863 at 60.983 kb on - strand, within GFF3863 at 60.983 kb on - strand, within GFF3863 at 60.983 kb on - strand, within GFF3863 at 60.983 kb on - strand, within GFF3863 at 60.983 kb on - strand, within GFF3863 at 60.983 kb on - strand, within GFF3863 at 60.983 kb on - strand, within GFF3863 at 60.983 kb on - strand, within GFF3863 at 60.995 kb on - strand, within GFF3863 at 61.102 kb on + strand, within GFF3863 at 61.102 kb on + strand, within GFF3863 at 61.102 kb on + strand, within GFF3863 at 61.103 kb on - strand, within GFF3863 at 61.103 kb on - strand, within GFF3863 at 61.252 kb on + strand, within GFF3863 at 61.252 kb on + strand, within GFF3863 at 61.252 kb on + strand, within GFF3863 at 61.252 kb on + strand, within GFF3863 at 61.252 kb on + strand, within GFF3863 at 61.252 kb on + strand, within GFF3863 at 61.253 kb on - strand, within GFF3863 at 61.253 kb on - strand, within GFF3863 at 61.253 kb on - strand, within GFF3863 at 61.253 kb on - strand, within GFF3863 at 61.253 kb on - strand, within GFF3863 at 61.253 kb on - strand, within GFF3863 at 61.253 kb on - strand, within GFF3863 at 61.378 kb on + strand at 61.378 kb on + strand at 61.378 kb on + strand at 61.378 kb on + strand at 61.378 kb on + strand at 61.378 kb on + strand at 61.378 kb on + strand at 61.378 kb on + strand at 61.378 kb on + strand at 61.378 kb on + strand at 61.378 kb on + strand at 61.378 kb on + strand at 61.379 kb on - strand at 61.379 kb on - strand at 61.379 kb on - strand at 61.379 kb on - strand at 61.379 kb on - strand at 61.379 kb on - strand at 61.379 kb on - strand at 61.379 kb on - strand at 61.379 kb on - strand at 61.379 kb on - strand at 61.379 kb on - strand at 61.382 kb on + strand at 61.382 kb on + strand at 61.382 kb on + strand at 61.382 kb on + strand at 61.382 kb on + strand at 61.382 kb on + strand at 61.382 kb on + strand at 61.382 kb on + strand at 61.382 kb on + strand at 61.382 kb on + strand at 61.382 kb on + strand at 61.382 kb on + strand at 61.382 kb on + strand at 61.382 kb on + strand at 61.382 kb on + strand at 61.382 kb on + strand at 61.382 kb on + strand at 61.382 kb on + strand at 61.382 kb on + strand at 61.382 kb on + strand at 61.382 kb on + strand at 61.383 kb on - strand at 61.383 kb on - strand at 61.383 kb on - strand at 61.383 kb on - strand at 61.383 kb on - strand at 61.383 kb on - strand at 61.383 kb on - strand at 61.383 kb on - strand at 61.383 kb on - strand at 61.383 kb on - strand at 61.383 kb on - strand at 61.383 kb on - strand at 61.383 kb on - strand at 61.383 kb on - strand at 61.383 kb on - strand at 61.383 kb on - strand at 61.383 kb on - strand at 61.383 kb on - strand at 61.383 kb on - strand at 61.383 kb on - strand at 61.383 kb on - strand at 61.383 kb on - strand at 61.383 kb on - strand at 61.383 kb on - strand at 61.383 kb on - strand at 61.383 kb on - strand at 61.383 kb on - strand at 61.383 kb on - strand at 61.383 kb on - strand at 61.527 kb on + strand, within GFF3864 at 61.527 kb on + strand, within GFF3864 at 61.527 kb on + strand, within GFF3864 at 61.527 kb on + strand, within GFF3864 at 61.527 kb on + strand, within GFF3864 at 61.527 kb on + strand, within GFF3864 at 61.528 kb on - strand, within GFF3864 at 61.528 kb on - strand, within GFF3864 at 61.528 kb on - strand, within GFF3864 at 61.528 kb on - strand, within GFF3864 at 61.714 kb on + strand, within GFF3864 at 61.715 kb on - strand, within GFF3864 at 61.715 kb on - strand, within GFF3864 at 61.715 kb on - strand, within GFF3864 at 62.087 kb on + strand at 62.087 kb on + strand at 62.087 kb on + strand at 62.101 kb on + strand at 62.101 kb on + strand at 62.102 kb on - strand at 62.102 kb on - strand at 62.102 kb on - strand at 62.102 kb on - strand at 62.304 kb on + strand at 62.304 kb on + strand at 62.304 kb on + strand at 62.304 kb on + strand at 62.304 kb on + strand at 62.304 kb on + strand at 62.304 kb on + strand at 62.304 kb on + strand at 62.304 kb on + strand at 62.304 kb on + strand at 62.304 kb on + strand at 62.304 kb on + strand at 62.304 kb on + strand at 62.304 kb on + strand at 62.304 kb on + strand at 62.304 kb on + strand at 62.305 kb on - strand at 62.305 kb on - strand at 62.305 kb on - strand at 62.305 kb on - strand at 62.305 kb on - strand at 62.305 kb on - strand at 62.305 kb on - strand at 62.305 kb on - strand at 62.305 kb on - strand at 62.305 kb on - strand at 62.305 kb on - strand at 62.305 kb on - strand at 62.305 kb on - strand at 62.305 kb on - strand at 62.305 kb on - strand at 62.305 kb on - strand at 62.305 kb on - strand at 62.556 kb on + strand, within GFF3865 at 62.557 kb on - strand, within GFF3865 at 62.557 kb on - strand, within GFF3865 at 62.557 kb on - strand, within GFF3865 at 62.805 kb on + strand, within GFF3865 at 63.024 kb on + strand, within GFF3865 at 63.024 kb on + strand, within GFF3865 at 63.024 kb on + strand, within GFF3865 at 63.025 kb on - strand, within GFF3865
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 60,430 + GFF3862 0.46 -0.1 60,619 + GFF3862 0.85 -1.5 60,619 + GFF3862 0.85 -0.3 60,619 + GFF3862 0.85 -0.7 60,619 + GFF3862 0.85 -1.5 60,619 + GFF3862 0.85 -0.1 60,619 + GFF3862 0.85 +0.9 60,619 + GFF3862 0.85 +0.2 60,619 + GFF3862 0.85 +1.5 60,619 + GFF3862 0.85 -0.2 60,620 - GFF3862 0.85 -0.5 60,620 - GFF3862 0.85 -2.7 60,620 - GFF3862 0.85 -0.4 60,620 - GFF3862 0.85 +2.1 60,620 - GFF3862 0.85 +1.2 60,621 + GFF3862 0.85 -0.1 60,621 + GFF3862 0.85 -0.1 60,621 + GFF3862 0.85 -0.7 60,621 + GFF3862 0.85 +0.4 60,621 + GFF3862 0.85 -1.5 60,621 + GFF3862 0.85 -0.4 60,622 - GFF3862 0.85 -2.1 60,622 - GFF3862 0.85 +0.0 60,622 - GFF3862 0.85 +0.4 60,622 - GFF3862 0.85 +0.5 60,622 - GFF3862 0.85 +0.4 60,622 - GFF3862 0.85 +0.6 60,622 - GFF3862 0.85 -0.3 60,622 - GFF3862 0.85 -0.1 60,622 - GFF3862 0.85 -0.3 60,622 - GFF3862 0.85 -0.9 60,622 - GFF3862 0.85 -0.1 60,622 - GFF3862 0.85 -0.7 60,622 - GFF3862 0.85 -0.8 60,622 - GFF3862 0.85 +2.3 60,622 - GFF3862 0.85 -0.4 60,622 - GFF3862 0.85 -0.4 60,622 - GFF3862 0.85 -0.8 60,622 - GFF3862 0.85 -0.2 60,622 - GFF3862 0.85 -0.7 60,622 - GFF3862 0.85 -0.2 60,622 - GFF3862 0.85 +0.1 60,622 - GFF3862 0.85 +1.5 60,709 + -0.1 60,853 + GFF3863 0.22 -1.7 60,853 + GFF3863 0.22 -0.3 60,853 + GFF3863 0.22 -0.5 60,853 + GFF3863 0.22 -0.3 60,853 + GFF3863 0.22 +0.9 60,853 + GFF3863 0.22 +2.5 60,854 - GFF3863 0.22 +0.3 60,854 - GFF3863 0.22 +0.1 60,854 - GFF3863 0.22 -0.4 60,854 - GFF3863 0.22 -1.2 60,854 - GFF3863 0.22 +0.3 60,854 - GFF3863 0.22 -0.2 60,854 - GFF3863 0.22 +0.3 60,854 - GFF3863 0.22 -0.3 60,916 - GFF3863 0.31 -0.6 60,982 + GFF3863 0.41 +0.5 60,982 + GFF3863 0.41 +0.6 60,982 + GFF3863 0.41 -0.5 60,982 + GFF3863 0.41 -0.1 60,982 + GFF3863 0.41 -1.7 60,982 + GFF3863 0.41 +0.0 60,982 + GFF3863 0.41 -0.5 60,982 + GFF3863 0.41 -1.8 60,982 + GFF3863 0.41 +0.1 60,982 + GFF3863 0.41 -0.7 60,982 + GFF3863 0.41 -0.5 60,982 + GFF3863 0.41 +0.5 60,982 + GFF3863 0.41 -0.5 60,982 + GFF3863 0.41 -1.0 60,983 - GFF3863 0.41 -0.3 60,983 - GFF3863 0.41 -1.2 60,983 - GFF3863 0.41 -0.0 60,983 - GFF3863 0.41 -0.2 60,983 - GFF3863 0.41 +0.4 60,983 - GFF3863 0.41 -0.6 60,983 - GFF3863 0.41 +0.3 60,983 - GFF3863 0.41 +0.3 60,983 - GFF3863 0.41 -0.7 60,983 - GFF3863 0.41 +1.1 60,983 - GFF3863 0.41 +0.8 60,983 - GFF3863 0.41 -0.7 60,983 - GFF3863 0.41 +0.2 60,995 - GFF3863 0.43 -0.1 61,102 + GFF3863 0.59 +0.3 61,102 + GFF3863 0.59 -0.7 61,102 + GFF3863 0.59 +0.9 61,103 - GFF3863 0.59 -1.7 61,103 - GFF3863 0.59 -0.7 61,252 + GFF3863 0.81 -1.5 61,252 + GFF3863 0.81 -1.5 61,252 + GFF3863 0.81 -0.1 61,252 + GFF3863 0.81 -0.4 61,252 + GFF3863 0.81 -0.5 61,252 + GFF3863 0.81 +0.0 61,253 - GFF3863 0.81 -0.2 61,253 - GFF3863 0.81 +0.1 61,253 - GFF3863 0.81 -0.7 61,253 - GFF3863 0.81 -0.1 61,253 - GFF3863 0.81 -0.2 61,253 - GFF3863 0.81 -0.9 61,253 - GFF3863 0.81 -0.4 61,378 + -0.8 61,378 + -0.7 61,378 + +0.5 61,378 + -0.4 61,378 + +0.9 61,378 + -0.2 61,378 + +0.3 61,378 + -0.6 61,378 + -0.9 61,378 + -1.1 61,378 + +0.6 61,378 + -0.1 61,379 - -1.1 61,379 - -0.5 61,379 - -0.3 61,379 - -0.3 61,379 - -1.3 61,379 - -1.1 61,379 - -0.7 61,379 - -0.4 61,379 - -0.5 61,379 - +0.1 61,379 - +0.9 61,382 + +0.9 61,382 + +0.0 61,382 + -0.2 61,382 + -0.6 61,382 + -0.2 61,382 + +0.1 61,382 + +1.8 61,382 + -0.7 61,382 + -1.1 61,382 + -0.1 61,382 + -1.2 61,382 + +0.2 61,382 + -0.2 61,382 + -3.3 61,382 + +0.7 61,382 + -0.2 61,382 + +1.5 61,382 + +0.7 61,382 + -0.6 61,382 + +1.0 61,382 + +1.8 61,383 - -0.7 61,383 - +0.5 61,383 - -0.6 61,383 - +0.5 61,383 - -0.4 61,383 - +0.2 61,383 - -0.6 61,383 - -0.5 61,383 - -0.3 61,383 - -0.6 61,383 - -0.7 61,383 - -0.6 61,383 - -1.5 61,383 - -2.0 61,383 - +0.5 61,383 - +0.2 61,383 - -0.8 61,383 - -0.2 61,383 - +0.1 61,383 - -0.8 61,383 - -1.5 61,383 - -0.4 61,383 - -0.5 61,383 - -1.4 61,383 - -1.1 61,383 - -0.1 61,383 - -0.0 61,383 - -0.8 61,383 - -2.7 61,527 + GFF3864 0.19 -1.2 61,527 + GFF3864 0.19 -0.4 61,527 + GFF3864 0.19 -0.2 61,527 + GFF3864 0.19 -0.5 61,527 + GFF3864 0.19 -1.5 61,527 + GFF3864 0.19 +1.5 61,528 - GFF3864 0.19 -0.8 61,528 - GFF3864 0.19 +0.5 61,528 - GFF3864 0.19 -0.6 61,528 - GFF3864 0.19 +1.1 61,714 + GFF3864 0.47 -0.5 61,715 - GFF3864 0.47 +0.8 61,715 - GFF3864 0.47 -0.6 61,715 - GFF3864 0.47 +0.0 62,087 + -1.5 62,087 + +0.7 62,087 + +0.5 62,101 + -0.4 62,101 + -0.2 62,102 - -1.1 62,102 - +0.8 62,102 - +0.5 62,102 - -1.0 62,304 + -1.1 62,304 + -0.8 62,304 + -0.1 62,304 + -1.0 62,304 + +0.0 62,304 + -0.3 62,304 + +0.8 62,304 + -0.5 62,304 + -0.9 62,304 + -0.2 62,304 + +1.0 62,304 + -0.8 62,304 + +0.3 62,304 + -1.2 62,304 + -0.8 62,304 + -0.4 62,305 - +1.5 62,305 - +0.9 62,305 - -1.2 62,305 - -1.1 62,305 - -0.7 62,305 - -0.2 62,305 - -0.7 62,305 - -1.0 62,305 - +0.9 62,305 - -0.4 62,305 - +0.1 62,305 - +0.8 62,305 - +0.4 62,305 - +0.7 62,305 - -0.6 62,305 - -0.4 62,305 - -0.3 62,556 + GFF3865 0.32 +1.5 62,557 - GFF3865 0.33 -0.4 62,557 - GFF3865 0.33 -2.2 62,557 - GFF3865 0.33 -1.5 62,805 + GFF3865 0.59 -0.4 63,024 + GFF3865 0.82 -0.7 63,024 + GFF3865 0.82 -0.5 63,024 + GFF3865 0.82 -0.7 63,025 - GFF3865 0.82 -1.5
Or see this region's nucleotide sequence