Strain Fitness in Variovorax sp. SCN45 around GFF3784

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3783 and GFF3784 are separated by 142 nucleotidesGFF3784 and GFF3785 are separated by 25 nucleotidesGFF3785 and GFF3786 are separated by 85 nucleotides GFF3783 - bifunctional P-450:NADPH-P450 reductase 1, at 274,783 to 278,007 GFF3783 GFF3784 - ABC transporter, substrate-binding protein RSc1750, at 278,150 to 279,406 GFF3784 GFF3785 - Transcriptional regulator, AcrR family, at 279,432 to 280,127 GFF3785 GFF3786 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 280,213 to 281,826 GFF3786 Position (kb) 278 279 280Strain fitness (log2 ratio) -1 0 1 2at 277.157 kb on + strand, within GFF3783at 277.158 kb on - strand, within GFF3783at 277.158 kb on - strand, within GFF3783at 277.283 kb on + strand, within GFF3783at 277.284 kb on - strand, within GFF3783at 277.284 kb on - strand, within GFF3783at 277.284 kb on - strand, within GFF3783at 277.284 kb on - strand, within GFF3783at 277.367 kb on + strand, within GFF3783at 277.367 kb on + strand, within GFF3783at 277.367 kb on + strand, within GFF3783at 277.368 kb on - strand, within GFF3783at 277.368 kb on - strand, within GFF3783at 277.652 kb on + strand, within GFF3783at 277.653 kb on - strand, within GFF3783at 277.653 kb on - strand, within GFF3783at 277.653 kb on - strand, within GFF3783at 277.874 kb on + strandat 277.874 kb on + strandat 277.874 kb on + strandat 277.874 kb on + strandat 277.875 kb on - strandat 278.045 kb on - strandat 278.057 kb on - strandat 278.067 kb on - strandat 278.202 kb on + strandat 278.203 kb on - strandat 278.203 kb on - strandat 278.203 kb on - strandat 278.484 kb on + strand, within GFF3784at 278.484 kb on + strand, within GFF3784at 278.484 kb on + strand, within GFF3784at 278.484 kb on + strand, within GFF3784at 278.485 kb on - strand, within GFF3784at 278.485 kb on - strand, within GFF3784at 278.623 kb on - strand, within GFF3784at 278.635 kb on - strand, within GFF3784at 278.733 kb on + strand, within GFF3784at 278.733 kb on + strand, within GFF3784at 278.826 kb on + strand, within GFF3784at 278.826 kb on + strand, within GFF3784at 278.827 kb on - strand, within GFF3784at 278.853 kb on + strand, within GFF3784at 278.853 kb on + strand, within GFF3784at 278.853 kb on + strand, within GFF3784at 278.853 kb on + strand, within GFF3784at 278.854 kb on - strand, within GFF3784at 278.854 kb on - strand, within GFF3784at 278.854 kb on - strand, within GFF3784at 278.854 kb on - strand, within GFF3784at 278.871 kb on + strand, within GFF3784at 278.871 kb on + strand, within GFF3784at 278.871 kb on + strand, within GFF3784at 278.871 kb on + strand, within GFF3784at 278.872 kb on - strand, within GFF3784at 278.872 kb on - strand, within GFF3784at 278.905 kb on - strand, within GFF3784at 278.905 kb on - strand, within GFF3784at 278.905 kb on - strand, within GFF3784at 278.905 kb on - strand, within GFF3784at 279.243 kb on + strand, within GFF3784at 279.243 kb on + strand, within GFF3784at 279.318 kb on + strandat 279.318 kb on + strandat 279.318 kb on + strandat 279.319 kb on - strandat 279.319 kb on - strandat 279.523 kb on + strand, within GFF3785at 279.524 kb on - strand, within GFF3785at 279.640 kb on + strand, within GFF3785at 279.640 kb on + strand, within GFF3785at 279.640 kb on + strand, within GFF3785at 279.641 kb on - strand, within GFF3785at 279.641 kb on - strand, within GFF3785at 279.677 kb on - strand, within GFF3785at 279.883 kb on + strand, within GFF3785at 279.883 kb on + strand, within GFF3785at 279.884 kb on - strand, within GFF3785at 279.886 kb on + strand, within GFF3785at 279.886 kb on + strand, within GFF3785at 279.886 kb on + strand, within GFF3785at 279.886 kb on + strand, within GFF3785at 279.887 kb on - strand, within GFF3785at 279.887 kb on - strand, within GFF3785at 279.889 kb on + strand, within GFF3785at 279.890 kb on - strand, within GFF3785at 280.119 kb on + strandat 280.119 kb on + strandat 280.132 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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277,157 + GFF3783 0.74 -0.2
277,158 - GFF3783 0.74 +0.3
277,158 - GFF3783 0.74 -0.3
277,283 + GFF3783 0.78 -0.6
277,284 - GFF3783 0.78 +0.4
277,284 - GFF3783 0.78 +0.5
277,284 - GFF3783 0.78 +0.6
277,284 - GFF3783 0.78 +0.6
277,367 + GFF3783 0.80 -0.9
277,367 + GFF3783 0.80 -1.4
277,367 + GFF3783 0.80 -0.1
277,368 - GFF3783 0.80 -0.4
277,368 - GFF3783 0.80 +0.9
277,652 + GFF3783 0.89 +0.3
277,653 - GFF3783 0.89 +0.3
277,653 - GFF3783 0.89 -0.6
277,653 - GFF3783 0.89 -0.5
277,874 + -0.3
277,874 + -0.3
277,874 + -1.2
277,874 + -0.6
277,875 - -0.6
278,045 - -1.0
278,057 - -0.6
278,067 - -0.5
278,202 + -1.0
278,203 - +0.6
278,203 - +0.6
278,203 - +0.8
278,484 + GFF3784 0.27 +0.0
278,484 + GFF3784 0.27 -1.1
278,484 + GFF3784 0.27 -0.1
278,484 + GFF3784 0.27 -0.1
278,485 - GFF3784 0.27 -0.5
278,485 - GFF3784 0.27 -0.3
278,623 - GFF3784 0.38 -1.4
278,635 - GFF3784 0.39 +0.1
278,733 + GFF3784 0.46 +0.8
278,733 + GFF3784 0.46 +2.4
278,826 + GFF3784 0.54 -0.4
278,826 + GFF3784 0.54 -0.6
278,827 - GFF3784 0.54 -0.6
278,853 + GFF3784 0.56 +0.3
278,853 + GFF3784 0.56 -0.3
278,853 + GFF3784 0.56 +1.1
278,853 + GFF3784 0.56 -0.1
278,854 - GFF3784 0.56 -0.4
278,854 - GFF3784 0.56 -1.1
278,854 - GFF3784 0.56 -0.3
278,854 - GFF3784 0.56 -0.5
278,871 + GFF3784 0.57 +2.2
278,871 + GFF3784 0.57 -0.2
278,871 + GFF3784 0.57 -0.2
278,871 + GFF3784 0.57 -0.3
278,872 - GFF3784 0.57 -0.3
278,872 - GFF3784 0.57 -1.0
278,905 - GFF3784 0.60 -0.5
278,905 - GFF3784 0.60 -0.7
278,905 - GFF3784 0.60 -0.4
278,905 - GFF3784 0.60 -0.6
279,243 + GFF3784 0.87 +0.0
279,243 + GFF3784 0.87 -0.1
279,318 + -0.6
279,318 + -0.1
279,318 + +1.8
279,319 - +0.3
279,319 - +0.1
279,523 + GFF3785 0.13 +0.2
279,524 - GFF3785 0.13 -1.5
279,640 + GFF3785 0.30 -0.3
279,640 + GFF3785 0.30 -0.1
279,640 + GFF3785 0.30 +0.4
279,641 - GFF3785 0.30 +0.8
279,641 - GFF3785 0.30 +0.2
279,677 - GFF3785 0.35 -0.4
279,883 + GFF3785 0.65 -0.3
279,883 + GFF3785 0.65 +1.8
279,884 - GFF3785 0.65 -0.8
279,886 + GFF3785 0.65 -1.5
279,886 + GFF3785 0.65 -0.2
279,886 + GFF3785 0.65 -0.6
279,886 + GFF3785 0.65 +0.2
279,887 - GFF3785 0.65 +0.0
279,887 - GFF3785 0.65 -0.7
279,889 + GFF3785 0.66 +0.9
279,890 - GFF3785 0.66 +0.3
280,119 + -0.5
280,119 + -1.3
280,132 - -0.3

Or see this region's nucleotide sequence