Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3643 and GFF3644 are separated by 9 nucleotides GFF3644 and GFF3645 are separated by 82 nucleotides GFF3645 and GFF3646 are separated by 5 nucleotides GFF3646 and GFF3647 overlap by 4 nucleotides GFF3647 and GFF3648 are separated by 155 nucleotides
GFF3643 - LSU ribosomal protein L29p (L35e), at 151,288 to 151,539
GFF3643
GFF3644 - SSU ribosomal protein S17p (S11e), at 151,549 to 151,818
GFF3644
GFF3645 - Peroxiredoxin, at 151,901 to 152,407
GFF3645
GFF3646 - Histone acetyltransferase HPA2 and related acetyltransferases, at 152,413 to 153,000
GFF3646
GFF3647 - Cytochrome b, at 152,997 to 153,710
GFF3647
GFF3648 - putative c'cytochrome, at 153,866 to 154,312
GFF3648
Position (kb) 152
153
154 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 151.946 kb on + strand at 151.946 kb on + strand at 151.946 kb on + strand at 151.946 kb on + strand at 151.946 kb on + strand at 151.946 kb on + strand at 151.946 kb on + strand at 151.947 kb on - strand at 151.947 kb on - strand at 151.947 kb on - strand at 151.947 kb on - strand at 151.947 kb on - strand at 151.956 kb on + strand, within GFF3645 at 152.087 kb on + strand, within GFF3645 at 152.087 kb on + strand, within GFF3645 at 152.087 kb on + strand, within GFF3645 at 152.087 kb on + strand, within GFF3645 at 152.087 kb on + strand, within GFF3645 at 152.087 kb on + strand, within GFF3645 at 152.087 kb on + strand, within GFF3645 at 152.087 kb on + strand, within GFF3645 at 152.088 kb on - strand, within GFF3645 at 152.088 kb on - strand, within GFF3645 at 152.088 kb on - strand, within GFF3645 at 152.088 kb on - strand, within GFF3645 at 152.300 kb on + strand, within GFF3645 at 152.300 kb on + strand, within GFF3645 at 152.301 kb on - strand, within GFF3645 at 152.432 kb on + strand at 152.432 kb on + strand at 152.432 kb on + strand at 152.433 kb on - strand at 152.433 kb on - strand at 152.433 kb on - strand at 152.433 kb on - strand at 152.433 kb on - strand at 152.433 kb on - strand at 152.433 kb on - strand at 152.433 kb on - strand at 152.433 kb on - strand at 152.459 kb on + strand at 152.459 kb on + strand at 152.459 kb on + strand at 152.459 kb on + strand at 152.459 kb on + strand at 152.459 kb on + strand at 152.459 kb on + strand at 152.459 kb on + strand at 152.459 kb on + strand at 152.459 kb on + strand at 152.459 kb on + strand at 152.459 kb on + strand at 152.459 kb on + strand at 152.459 kb on + strand at 152.460 kb on - strand at 152.460 kb on - strand at 152.460 kb on - strand at 152.460 kb on - strand at 152.460 kb on - strand at 152.460 kb on - strand at 152.460 kb on - strand at 152.460 kb on - strand at 152.460 kb on - strand at 152.460 kb on - strand at 152.460 kb on - strand at 152.460 kb on - strand at 152.460 kb on - strand at 152.460 kb on - strand at 152.460 kb on - strand at 152.460 kb on - strand at 152.460 kb on - strand at 152.462 kb on + strand at 152.462 kb on + strand at 152.462 kb on + strand at 152.462 kb on + strand at 152.462 kb on + strand at 152.462 kb on + strand at 152.462 kb on + strand at 152.462 kb on + strand at 152.462 kb on + strand at 152.462 kb on + strand at 152.462 kb on + strand at 152.462 kb on + strand at 152.462 kb on + strand at 152.463 kb on - strand at 152.463 kb on - strand at 152.463 kb on - strand at 152.463 kb on - strand at 152.463 kb on - strand at 152.463 kb on - strand at 152.463 kb on - strand at 152.495 kb on + strand, within GFF3646 at 152.495 kb on + strand, within GFF3646 at 152.496 kb on - strand, within GFF3646 at 152.496 kb on - strand, within GFF3646 at 152.496 kb on - strand, within GFF3646 at 152.549 kb on + strand, within GFF3646 at 152.549 kb on + strand, within GFF3646 at 152.630 kb on + strand, within GFF3646 at 152.630 kb on + strand, within GFF3646 at 152.630 kb on + strand, within GFF3646 at 152.630 kb on + strand, within GFF3646 at 152.630 kb on + strand, within GFF3646 at 152.630 kb on + strand, within GFF3646 at 152.630 kb on + strand, within GFF3646 at 152.630 kb on + strand, within GFF3646 at 152.630 kb on + strand, within GFF3646 at 152.630 kb on + strand, within GFF3646 at 152.630 kb on + strand, within GFF3646 at 152.630 kb on + strand, within GFF3646 at 152.631 kb on - strand, within GFF3646 at 152.631 kb on - strand, within GFF3646 at 152.631 kb on - strand, within GFF3646 at 152.631 kb on - strand, within GFF3646 at 152.660 kb on + strand, within GFF3646 at 152.660 kb on + strand, within GFF3646 at 152.660 kb on + strand, within GFF3646 at 152.660 kb on + strand, within GFF3646 at 152.660 kb on + strand, within GFF3646 at 152.661 kb on - strand, within GFF3646 at 152.661 kb on - strand, within GFF3646 at 152.661 kb on - strand, within GFF3646 at 152.661 kb on - strand, within GFF3646 at 152.816 kb on + strand, within GFF3646 at 152.817 kb on - strand, within GFF3646 at 152.817 kb on - strand, within GFF3646 at 152.817 kb on - strand, within GFF3646 at 152.817 kb on - strand, within GFF3646 at 152.889 kb on - strand, within GFF3646 at 152.889 kb on - strand, within GFF3646 at 152.889 kb on - strand, within GFF3646 at 152.889 kb on - strand, within GFF3646 at 153.000 kb on + strand at 153.000 kb on + strand at 153.000 kb on + strand at 153.000 kb on + strand at 153.000 kb on + strand at 153.000 kb on + strand at 153.000 kb on + strand at 153.001 kb on - strand at 153.001 kb on - strand at 153.001 kb on - strand at 153.001 kb on - strand at 153.001 kb on - strand at 153.001 kb on - strand at 153.001 kb on - strand at 153.001 kb on - strand at 153.001 kb on - strand at 153.001 kb on - strand at 153.001 kb on - strand at 153.001 kb on - strand at 153.001 kb on - strand at 153.022 kb on + strand at 153.022 kb on + strand at 153.023 kb on - strand at 153.023 kb on - strand at 153.120 kb on + strand, within GFF3647 at 153.120 kb on + strand, within GFF3647 at 153.120 kb on + strand, within GFF3647 at 153.120 kb on + strand, within GFF3647 at 153.120 kb on + strand, within GFF3647 at 153.120 kb on + strand, within GFF3647 at 153.120 kb on + strand, within GFF3647 at 153.121 kb on - strand, within GFF3647 at 153.121 kb on - strand, within GFF3647 at 153.121 kb on - strand, within GFF3647 at 153.121 kb on - strand, within GFF3647 at 153.121 kb on - strand, within GFF3647 at 153.121 kb on - strand, within GFF3647 at 153.121 kb on - strand, within GFF3647 at 153.178 kb on + strand, within GFF3647 at 153.178 kb on + strand, within GFF3647 at 153.178 kb on + strand, within GFF3647 at 153.179 kb on - strand, within GFF3647 at 153.179 kb on - strand, within GFF3647 at 153.179 kb on - strand, within GFF3647 at 153.179 kb on - strand, within GFF3647 at 153.179 kb on - strand, within GFF3647 at 153.207 kb on + strand, within GFF3647 at 153.207 kb on + strand, within GFF3647 at 153.208 kb on - strand, within GFF3647 at 153.208 kb on - strand, within GFF3647 at 153.208 kb on - strand, within GFF3647 at 153.208 kb on - strand, within GFF3647 at 153.208 kb on - strand, within GFF3647 at 153.208 kb on - strand, within GFF3647 at 153.436 kb on + strand, within GFF3647 at 153.436 kb on + strand, within GFF3647 at 153.436 kb on + strand, within GFF3647 at 153.436 kb on + strand, within GFF3647 at 153.437 kb on - strand, within GFF3647 at 153.437 kb on - strand, within GFF3647 at 153.437 kb on - strand, within GFF3647 at 153.437 kb on - strand, within GFF3647 at 153.437 kb on - strand, within GFF3647 at 153.460 kb on + strand, within GFF3647 at 153.460 kb on + strand, within GFF3647 at 153.460 kb on + strand, within GFF3647 at 153.460 kb on + strand, within GFF3647 at 153.460 kb on + strand, within GFF3647 at 153.460 kb on + strand, within GFF3647 at 153.460 kb on + strand, within GFF3647 at 153.460 kb on + strand, within GFF3647 at 153.461 kb on - strand, within GFF3647 at 153.461 kb on - strand, within GFF3647 at 153.461 kb on - strand, within GFF3647 at 153.461 kb on - strand, within GFF3647 at 153.541 kb on + strand, within GFF3647 at 153.541 kb on + strand, within GFF3647 at 153.541 kb on + strand, within GFF3647 at 153.542 kb on - strand, within GFF3647 at 153.753 kb on + strand at 153.753 kb on + strand at 153.753 kb on + strand at 153.754 kb on - strand at 153.754 kb on - strand at 153.754 kb on - strand at 153.754 kb on - strand at 153.791 kb on + strand at 153.791 kb on + strand at 153.791 kb on + strand at 153.791 kb on + strand at 153.791 kb on + strand at 153.791 kb on + strand at 153.791 kb on + strand at 153.792 kb on - strand at 153.792 kb on - strand at 153.792 kb on - strand at 153.792 kb on - strand at 153.815 kb on + strand at 153.815 kb on + strand at 153.815 kb on + strand at 153.815 kb on + strand at 153.815 kb on + strand at 153.815 kb on + strand at 153.815 kb on + strand at 153.815 kb on + strand at 153.815 kb on + strand at 153.816 kb on - strand at 153.816 kb on - strand at 153.816 kb on - strand at 153.816 kb on - strand at 153.962 kb on + strand, within GFF3648 at 153.962 kb on + strand, within GFF3648 at 153.962 kb on + strand, within GFF3648 at 153.962 kb on + strand, within GFF3648 at 153.962 kb on + strand, within GFF3648 at 153.963 kb on - strand, within GFF3648 at 153.963 kb on - strand, within GFF3648 at 153.963 kb on - strand, within GFF3648 at 153.963 kb on - strand, within GFF3648 at 153.963 kb on - strand, within GFF3648 at 153.963 kb on - strand, within GFF3648 at 153.963 kb on - strand, within GFF3648
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 151,946 + +0.0 151,946 + +0.3 151,946 + +2.8 151,946 + -0.5 151,946 + +0.2 151,946 + +0.3 151,946 + -0.6 151,947 - -0.2 151,947 - -1.4 151,947 - -0.3 151,947 - +1.1 151,947 - +1.3 151,956 + GFF3645 0.11 +0.5 152,087 + GFF3645 0.37 +0.2 152,087 + GFF3645 0.37 +0.2 152,087 + GFF3645 0.37 +0.0 152,087 + GFF3645 0.37 -0.2 152,087 + GFF3645 0.37 +0.6 152,087 + GFF3645 0.37 +0.3 152,087 + GFF3645 0.37 -0.2 152,087 + GFF3645 0.37 +0.8 152,088 - GFF3645 0.37 -0.9 152,088 - GFF3645 0.37 -0.1 152,088 - GFF3645 0.37 -0.5 152,088 - GFF3645 0.37 -0.2 152,300 + GFF3645 0.79 -0.1 152,300 + GFF3645 0.79 -0.0 152,301 - GFF3645 0.79 +0.3 152,432 + +1.2 152,432 + -0.4 152,432 + -0.3 152,433 - -0.5 152,433 - -0.3 152,433 - -0.6 152,433 - +0.2 152,433 - -0.5 152,433 - -0.3 152,433 - -1.4 152,433 - -0.7 152,433 - -0.4 152,459 + +0.0 152,459 + +0.4 152,459 + -2.2 152,459 + -0.3 152,459 + -0.4 152,459 + -0.8 152,459 + +2.5 152,459 + +0.1 152,459 + -0.9 152,459 + -0.6 152,459 + -0.3 152,459 + -0.6 152,459 + -0.5 152,459 + -1.0 152,460 - +0.2 152,460 - -0.5 152,460 - -0.0 152,460 - -0.9 152,460 - -0.7 152,460 - +1.1 152,460 - -0.5 152,460 - -0.7 152,460 - -2.0 152,460 - +0.1 152,460 - +1.2 152,460 - +2.3 152,460 - +0.0 152,460 - -0.2 152,460 - -0.6 152,460 - +0.8 152,460 - -0.6 152,462 + +0.5 152,462 + -0.8 152,462 + +0.4 152,462 + +0.4 152,462 + -0.5 152,462 + -0.3 152,462 + -1.0 152,462 + +0.3 152,462 + +1.8 152,462 + -0.6 152,462 + -0.9 152,462 + -1.2 152,462 + -0.2 152,463 - +0.4 152,463 - -1.7 152,463 - -0.0 152,463 - -0.3 152,463 - +0.8 152,463 - -0.1 152,463 - -1.0 152,495 + GFF3646 0.14 -1.3 152,495 + GFF3646 0.14 -0.1 152,496 - GFF3646 0.14 +1.2 152,496 - GFF3646 0.14 -0.2 152,496 - GFF3646 0.14 -0.6 152,549 + GFF3646 0.23 -0.6 152,549 + GFF3646 0.23 -0.7 152,630 + GFF3646 0.37 +0.4 152,630 + GFF3646 0.37 -0.9 152,630 + GFF3646 0.37 -1.2 152,630 + GFF3646 0.37 -1.2 152,630 + GFF3646 0.37 -1.6 152,630 + GFF3646 0.37 +1.8 152,630 + GFF3646 0.37 +0.1 152,630 + GFF3646 0.37 +0.1 152,630 + GFF3646 0.37 -2.4 152,630 + GFF3646 0.37 -0.3 152,630 + GFF3646 0.37 -0.3 152,630 + GFF3646 0.37 +0.0 152,631 - GFF3646 0.37 -2.6 152,631 - GFF3646 0.37 -0.1 152,631 - GFF3646 0.37 -0.2 152,631 - GFF3646 0.37 -0.8 152,660 + GFF3646 0.42 +0.4 152,660 + GFF3646 0.42 -0.2 152,660 + GFF3646 0.42 -0.0 152,660 + GFF3646 0.42 -0.6 152,660 + GFF3646 0.42 -0.5 152,661 - GFF3646 0.42 -0.8 152,661 - GFF3646 0.42 +0.3 152,661 - GFF3646 0.42 -0.2 152,661 - GFF3646 0.42 -0.0 152,816 + GFF3646 0.69 -0.3 152,817 - GFF3646 0.69 -0.4 152,817 - GFF3646 0.69 -0.9 152,817 - GFF3646 0.69 -0.5 152,817 - GFF3646 0.69 +0.3 152,889 - GFF3646 0.81 +0.3 152,889 - GFF3646 0.81 +0.1 152,889 - GFF3646 0.81 -0.5 152,889 - GFF3646 0.81 -1.1 153,000 + +0.1 153,000 + +0.2 153,000 + -1.0 153,000 + -0.4 153,000 + -0.2 153,000 + +0.5 153,000 + -0.2 153,001 - +1.8 153,001 - +0.6 153,001 - +0.6 153,001 - -0.8 153,001 - +1.0 153,001 - -1.3 153,001 - -0.6 153,001 - -0.5 153,001 - -0.4 153,001 - +0.2 153,001 - -0.2 153,001 - -0.5 153,001 - -1.2 153,022 + +0.2 153,022 + -0.4 153,023 - -0.5 153,023 - +0.6 153,120 + GFF3647 0.17 -0.3 153,120 + GFF3647 0.17 +0.8 153,120 + GFF3647 0.17 +0.4 153,120 + GFF3647 0.17 -0.3 153,120 + GFF3647 0.17 +0.8 153,120 + GFF3647 0.17 -0.2 153,120 + GFF3647 0.17 -0.8 153,121 - GFF3647 0.17 -0.4 153,121 - GFF3647 0.17 +0.2 153,121 - GFF3647 0.17 -0.6 153,121 - GFF3647 0.17 -1.0 153,121 - GFF3647 0.17 +0.2 153,121 - GFF3647 0.17 -1.4 153,121 - GFF3647 0.17 -0.6 153,178 + GFF3647 0.25 +1.0 153,178 + GFF3647 0.25 -0.8 153,178 + GFF3647 0.25 -0.5 153,179 - GFF3647 0.25 -2.8 153,179 - GFF3647 0.25 -1.8 153,179 - GFF3647 0.25 +0.2 153,179 - GFF3647 0.25 -1.2 153,179 - GFF3647 0.25 -0.8 153,207 + GFF3647 0.29 -0.6 153,207 + GFF3647 0.29 -0.2 153,208 - GFF3647 0.30 +0.2 153,208 - GFF3647 0.30 +0.4 153,208 - GFF3647 0.30 -0.0 153,208 - GFF3647 0.30 +0.6 153,208 - GFF3647 0.30 +0.8 153,208 - GFF3647 0.30 +0.4 153,436 + GFF3647 0.61 +0.3 153,436 + GFF3647 0.61 -0.4 153,436 + GFF3647 0.61 -1.2 153,436 + GFF3647 0.61 -0.4 153,437 - GFF3647 0.62 -0.4 153,437 - GFF3647 0.62 -0.6 153,437 - GFF3647 0.62 -0.2 153,437 - GFF3647 0.62 -0.6 153,437 - GFF3647 0.62 +0.1 153,460 + GFF3647 0.65 -0.1 153,460 + GFF3647 0.65 -0.6 153,460 + GFF3647 0.65 +0.1 153,460 + GFF3647 0.65 -0.4 153,460 + GFF3647 0.65 -0.1 153,460 + GFF3647 0.65 -0.5 153,460 + GFF3647 0.65 +0.3 153,460 + GFF3647 0.65 -1.6 153,461 - GFF3647 0.65 +1.4 153,461 - GFF3647 0.65 +1.3 153,461 - GFF3647 0.65 +0.2 153,461 - GFF3647 0.65 -0.5 153,541 + GFF3647 0.76 -0.3 153,541 + GFF3647 0.76 +0.7 153,541 + GFF3647 0.76 -1.0 153,542 - GFF3647 0.76 +1.0 153,753 + -1.9 153,753 + +0.2 153,753 + +0.4 153,754 - -0.4 153,754 - -0.6 153,754 - -0.9 153,754 - -0.3 153,791 + -1.1 153,791 + -0.4 153,791 + +0.1 153,791 + +0.6 153,791 + +1.1 153,791 + -0.0 153,791 + -1.0 153,792 - -0.0 153,792 - +0.2 153,792 - -0.6 153,792 - +1.8 153,815 + -0.2 153,815 + -0.1 153,815 + +1.5 153,815 + -0.9 153,815 + -0.5 153,815 + +0.2 153,815 + +0.3 153,815 + +0.6 153,815 + -0.3 153,816 - -0.7 153,816 - -1.0 153,816 - -2.0 153,816 - +0.2 153,962 + GFF3648 0.21 +0.2 153,962 + GFF3648 0.21 -0.0 153,962 + GFF3648 0.21 -0.9 153,962 + GFF3648 0.21 -0.2 153,962 + GFF3648 0.21 +0.1 153,963 - GFF3648 0.22 -1.0 153,963 - GFF3648 0.22 -1.0 153,963 - GFF3648 0.22 -0.5 153,963 - GFF3648 0.22 -0.3 153,963 - GFF3648 0.22 -0.5 153,963 - GFF3648 0.22 +0.8 153,963 - GFF3648 0.22 -0.0
Or see this region's nucleotide sequence