Strain Fitness in Variovorax sp. SCN45 around GFF3599

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
111,843	-		GFF3598	0.27	+0.4
112,199	+		GFF3598	0.59	-1.6
112,199	+		GFF3598	0.59	+0.1
112,199	+		GFF3598	0.59	-0.2
112,199	+		GFF3598	0.59	-0.0
112,199	+		GFF3598	0.59	+0.8
112,199	+		GFF3598	0.59	+0.3
112,199	+		GFF3598	0.59	-1.2
112,199	+		GFF3598	0.59	+0.1
112,199	+		GFF3598	0.59	+0.2
112,200	-		GFF3598	0.59	-0.5
112,200	-		GFF3598	0.59	+0.4
112,200	-		GFF3598	0.59	+0.4
112,200	-		GFF3598	0.59	+0.7
112,229	+		GFF3598	0.62	-0.4
112,229	+		GFF3598	0.62	-0.0
112,229	+		GFF3598	0.62	-0.0
112,229	+		GFF3598	0.62	-0.2
112,230	-		GFF3598	0.62	+0.6
112,230	-		GFF3598	0.62	+0.5
112,230	-		GFF3598	0.62	+0.4
112,230	-		GFF3598	0.62	+0.2
112,230	-		GFF3598	0.62	-0.0
112,230	-		GFF3598	0.62	-0.2
112,230	-		GFF3598	0.62	-1.2
112,262	+		GFF3598	0.65	-0.2
112,262	+		GFF3598	0.65	+0.5
112,262	+		GFF3598	0.65	-0.6
112,262	+		GFF3598	0.65	+0.4
112,263	-		GFF3598	0.65	+0.7
112,263	-		GFF3598	0.65	-0.2
112,263	-		GFF3598	0.65	-0.4
112,346	+		GFF3598	0.72	+0.0
112,347	-		GFF3598	0.72	+0.2
112,490	+		GFF3598	0.85	-0.3
112,491	-		GFF3598	0.85	+0.2
112,491	-		GFF3598	0.85	+0.4
112,503	-		GFF3598	0.86	+0.4
112,583	+				+0.2
112,711	+				+0.1
112,711	+				+0.4
112,712	-				+0.8
112,712	-				+0.2
112,712	-				-1.5
112,712	-				-0.6
112,858	+				-0.0
112,858	+				-0.8
112,859	-				-0.9
112,859	-				-0.2
112,859	-				+0.3
112,960	+		GFF3599	0.31	+0.7
112,961	-		GFF3599	0.32	+0.1
112,961	-		GFF3599	0.32	+1.0
113,140	+		GFF3599	0.75	+0.2
113,140	+		GFF3599	0.75	+0.4
113,140	+		GFF3599	0.75	+0.4
113,140	+		GFF3599	0.75	+0.5
113,140	+		GFF3599	0.75	-0.3
113,140	+		GFF3599	0.75	-0.7
113,140	+		GFF3599	0.75	+0.6
113,140	+		GFF3599	0.75	-0.4
113,140	+		GFF3599	0.75	+0.4
113,140	+		GFF3599	0.75	+0.1
113,140	+		GFF3599	0.75	+0.1
113,140	+		GFF3599	0.75	-0.0
113,140	+		GFF3599	0.75	+1.6
113,140	+		GFF3599	0.75	-0.3
113,140	+		GFF3599	0.75	+1.3
113,140	+		GFF3599	0.75	+0.6
113,141	-		GFF3599	0.75	-1.7
113,141	-		GFF3599	0.75	+0.2
113,141	-		GFF3599	0.75	+0.5
113,141	-		GFF3599	0.75	+0.0
113,141	-		GFF3599	0.75	+0.4
113,141	-		GFF3599	0.75	+1.4
113,141	-		GFF3599	0.75	-0.5
113,141	-		GFF3599	0.75	+1.8
113,141	-		GFF3599	0.75	-0.6
113,141	-		GFF3599	0.75	-0.1
113,141	-		GFF3599	0.75	+0.5
113,141	-		GFF3599	0.75	-0.2
113,141	-		GFF3599	0.75	+0.2
113,141	-		GFF3599	0.75	+0.3
113,141	-		GFF3599	0.75	-0.8
113,322	-				-1.6
113,368	-				-1.0
113,390	-				+0.2
113,926	+		GFF3600	0.76	+0.2
113,926	+		GFF3600	0.76	-1.2
113,926	+		GFF3600	0.76	-1.7
113,926	+		GFF3600	0.76	-0.4
113,926	+		GFF3600	0.76	-2.6
113,926	+		GFF3600	0.76	-0.2
113,926	+		GFF3600	0.76	+0.8
113,927	-		GFF3600	0.76	+1.8
113,927	-		GFF3600	0.76	-1.7
113,998	+		GFF3600	0.85	-2.1
113,998	+		GFF3600	0.85	-0.2
113,998	+		GFF3600	0.85	-0.2
113,999	-		GFF3600	0.86	-0.2
114,112	+				-0.7
114,113	-				+0.0