Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3539 and GFF3540 are separated by 131 nucleotides GFF3540 and GFF3541 overlap by 1 nucleotides GFF3541 and GFF3542 overlap by 4 nucleotides GFF3542 and GFF3543 are separated by 5 nucleotides
GFF3539 - Probable ALANIN-rich signal peptide protein, at 45,224 to 45,721
GFF3539
GFF3540 - hypothetical protein, at 45,853 to 46,515
GFF3540
GFF3541 - hypothetical protein, at 46,515 to 47,342
GFF3541
GFF3542 - hypothetical protein, at 47,339 to 47,851
GFF3542
GFF3543 - no description, at 47,857 to 48,453
GFF3543
Position (kb)
46
47
48 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 45.594 kb on + strand, within GFF3539 at 45.594 kb on + strand, within GFF3539 at 45.594 kb on + strand, within GFF3539 at 45.594 kb on + strand, within GFF3539 at 45.594 kb on + strand, within GFF3539 at 45.594 kb on + strand, within GFF3539 at 45.594 kb on + strand, within GFF3539 at 45.594 kb on + strand, within GFF3539 at 45.594 kb on + strand, within GFF3539 at 45.594 kb on + strand, within GFF3539 at 45.594 kb on + strand, within GFF3539 at 45.595 kb on - strand, within GFF3539 at 45.595 kb on - strand, within GFF3539 at 45.595 kb on - strand, within GFF3539 at 45.595 kb on - strand, within GFF3539 at 45.595 kb on - strand, within GFF3539 at 45.595 kb on - strand, within GFF3539 at 45.595 kb on - strand, within GFF3539 at 45.595 kb on - strand, within GFF3539 at 45.595 kb on - strand, within GFF3539 at 45.595 kb on - strand, within GFF3539 at 45.764 kb on + strand at 45.764 kb on + strand at 45.823 kb on + strand at 45.823 kb on + strand at 45.823 kb on + strand at 45.823 kb on + strand at 45.824 kb on - strand at 45.824 kb on - strand at 45.824 kb on - strand at 45.890 kb on + strand at 45.890 kb on + strand at 45.890 kb on + strand at 45.891 kb on - strand at 45.891 kb on - strand at 45.891 kb on - strand at 45.891 kb on - strand at 45.923 kb on + strand, within GFF3540 at 45.923 kb on + strand, within GFF3540 at 46.260 kb on + strand, within GFF3540 at 46.260 kb on + strand, within GFF3540 at 46.260 kb on + strand, within GFF3540 at 46.585 kb on + strand at 46.586 kb on - strand at 46.774 kb on + strand, within GFF3541 at 46.774 kb on + strand, within GFF3541 at 46.774 kb on + strand, within GFF3541 at 46.774 kb on + strand, within GFF3541 at 46.774 kb on + strand, within GFF3541 at 46.774 kb on + strand, within GFF3541 at 46.775 kb on - strand, within GFF3541 at 46.775 kb on - strand, within GFF3541 at 46.775 kb on - strand, within GFF3541 at 46.775 kb on - strand, within GFF3541 at 46.775 kb on - strand, within GFF3541 at 46.775 kb on - strand, within GFF3541 at 46.775 kb on - strand, within GFF3541 at 46.855 kb on + strand, within GFF3541 at 46.855 kb on + strand, within GFF3541 at 46.855 kb on + strand, within GFF3541 at 46.855 kb on + strand, within GFF3541 at 46.855 kb on + strand, within GFF3541 at 46.855 kb on + strand, within GFF3541 at 46.855 kb on + strand, within GFF3541 at 46.855 kb on + strand, within GFF3541 at 46.855 kb on + strand, within GFF3541 at 46.855 kb on + strand, within GFF3541 at 46.855 kb on + strand, within GFF3541 at 46.855 kb on + strand, within GFF3541 at 46.855 kb on + strand, within GFF3541 at 46.855 kb on + strand, within GFF3541 at 46.856 kb on - strand, within GFF3541 at 46.856 kb on - strand, within GFF3541 at 46.856 kb on - strand, within GFF3541 at 46.856 kb on - strand, within GFF3541 at 46.856 kb on - strand, within GFF3541 at 46.856 kb on - strand, within GFF3541 at 46.856 kb on - strand, within GFF3541 at 46.856 kb on - strand, within GFF3541 at 46.856 kb on - strand, within GFF3541 at 46.927 kb on + strand, within GFF3541 at 47.102 kb on - strand, within GFF3541 at 47.125 kb on + strand, within GFF3541 at 47.125 kb on + strand, within GFF3541 at 47.125 kb on + strand, within GFF3541 at 47.125 kb on + strand, within GFF3541 at 47.126 kb on - strand, within GFF3541 at 47.126 kb on - strand, within GFF3541 at 47.354 kb on + strand at 47.354 kb on + strand at 47.354 kb on + strand at 47.355 kb on - strand at 47.355 kb on - strand at 47.365 kb on - strand at 47.365 kb on - strand at 47.365 kb on - strand at 47.592 kb on + strand, within GFF3542 at 47.592 kb on + strand, within GFF3542 at 47.592 kb on + strand, within GFF3542 at 47.592 kb on + strand, within GFF3542 at 47.592 kb on + strand, within GFF3542 at 47.592 kb on + strand, within GFF3542 at 47.592 kb on + strand, within GFF3542 at 47.592 kb on + strand, within GFF3542 at 47.592 kb on + strand, within GFF3542 at 47.592 kb on + strand, within GFF3542 at 47.592 kb on + strand, within GFF3542 at 47.592 kb on + strand, within GFF3542 at 47.592 kb on + strand, within GFF3542 at 47.593 kb on - strand, within GFF3542 at 47.593 kb on - strand, within GFF3542 at 47.593 kb on - strand, within GFF3542 at 47.593 kb on - strand, within GFF3542 at 47.593 kb on - strand, within GFF3542 at 47.593 kb on - strand, within GFF3542 at 47.595 kb on + strand, within GFF3542 at 47.595 kb on + strand, within GFF3542 at 47.595 kb on + strand, within GFF3542 at 47.595 kb on + strand, within GFF3542 at 47.595 kb on + strand, within GFF3542 at 47.595 kb on + strand, within GFF3542 at 47.595 kb on + strand, within GFF3542 at 47.595 kb on + strand, within GFF3542 at 47.595 kb on + strand, within GFF3542 at 47.596 kb on - strand, within GFF3542 at 47.596 kb on - strand, within GFF3542 at 47.596 kb on - strand, within GFF3542 at 47.596 kb on - strand, within GFF3542 at 47.596 kb on - strand, within GFF3542 at 47.596 kb on - strand, within GFF3542 at 47.596 kb on - strand, within GFF3542 at 47.596 kb on - strand, within GFF3542 at 47.596 kb on - strand, within GFF3542 at 47.619 kb on + strand, within GFF3542 at 47.619 kb on + strand, within GFF3542 at 47.619 kb on + strand, within GFF3542 at 47.619 kb on + strand, within GFF3542 at 47.619 kb on + strand, within GFF3542 at 47.620 kb on - strand, within GFF3542 at 47.620 kb on - strand, within GFF3542 at 47.620 kb on - strand, within GFF3542 at 47.894 kb on + strand at 47.894 kb on + strand at 47.894 kb on + strand at 47.894 kb on + strand at 47.895 kb on - strand at 47.895 kb on - strand at 47.909 kb on + strand at 47.909 kb on + strand at 47.910 kb on - strand at 47.927 kb on + strand, within GFF3543 at 47.927 kb on + strand, within GFF3543 at 47.928 kb on - strand, within GFF3543 at 47.928 kb on - strand, within GFF3543 at 47.928 kb on - strand, within GFF3543 at 48.049 kb on + strand, within GFF3543 at 48.049 kb on + strand, within GFF3543 at 48.049 kb on + strand, within GFF3543 at 48.049 kb on + strand, within GFF3543 at 48.049 kb on + strand, within GFF3543 at 48.049 kb on + strand, within GFF3543 at 48.049 kb on + strand, within GFF3543 at 48.049 kb on + strand, within GFF3543 at 48.050 kb on - strand, within GFF3543 at 48.050 kb on - strand, within GFF3543 at 48.050 kb on - strand, within GFF3543 at 48.050 kb on - strand, within GFF3543 at 48.050 kb on - strand, within GFF3543 at 48.050 kb on - strand, within GFF3543 at 48.050 kb on - strand, within GFF3543 at 48.050 kb on - strand, within GFF3543 at 48.050 kb on - strand, within GFF3543 at 48.050 kb on - strand, within GFF3543 at 48.331 kb on + strand, within GFF3543 at 48.331 kb on + strand, within GFF3543 at 48.331 kb on + strand, within GFF3543 at 48.331 kb on + strand, within GFF3543 at 48.332 kb on - strand, within GFF3543 at 48.332 kb on - strand, within GFF3543 at 48.332 kb on - strand, within GFF3543
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 45,594 + GFF3539 0.74 +1.4 45,594 + GFF3539 0.74 +0.0 45,594 + GFF3539 0.74 +0.0 45,594 + GFF3539 0.74 +0.3 45,594 + GFF3539 0.74 -0.1 45,594 + GFF3539 0.74 -0.3 45,594 + GFF3539 0.74 -0.2 45,594 + GFF3539 0.74 +0.5 45,594 + GFF3539 0.74 -0.4 45,594 + GFF3539 0.74 -0.2 45,594 + GFF3539 0.74 +0.3 45,595 - GFF3539 0.74 +0.1 45,595 - GFF3539 0.74 -0.5 45,595 - GFF3539 0.74 -0.9 45,595 - GFF3539 0.74 -0.4 45,595 - GFF3539 0.74 -0.0 45,595 - GFF3539 0.74 -0.8 45,595 - GFF3539 0.74 +0.1 45,595 - GFF3539 0.74 +0.0 45,595 - GFF3539 0.74 -0.2 45,595 - GFF3539 0.74 -1.0 45,764 + -0.3 45,764 + -0.2 45,823 + +0.2 45,823 + +0.2 45,823 + +0.1 45,823 + +0.3 45,824 - +0.3 45,824 - +1.2 45,824 - +0.8 45,890 + +0.8 45,890 + -0.1 45,890 + -0.4 45,891 - -1.0 45,891 - -0.8 45,891 - -0.7 45,891 - -0.9 45,923 + GFF3540 0.11 +0.4 45,923 + GFF3540 0.11 -1.5 46,260 + GFF3540 0.61 -0.5 46,260 + GFF3540 0.61 +0.8 46,260 + GFF3540 0.61 -0.7 46,585 + +0.9 46,586 - -1.7 46,774 + GFF3541 0.31 +0.1 46,774 + GFF3541 0.31 -0.1 46,774 + GFF3541 0.31 -0.5 46,774 + GFF3541 0.31 -0.0 46,774 + GFF3541 0.31 +0.2 46,774 + GFF3541 0.31 +1.0 46,775 - GFF3541 0.31 -0.6 46,775 - GFF3541 0.31 +0.9 46,775 - GFF3541 0.31 -0.6 46,775 - GFF3541 0.31 +1.2 46,775 - GFF3541 0.31 -0.8 46,775 - GFF3541 0.31 +1.4 46,775 - GFF3541 0.31 +0.2 46,855 + GFF3541 0.41 -0.3 46,855 + GFF3541 0.41 +0.1 46,855 + GFF3541 0.41 -0.2 46,855 + GFF3541 0.41 +0.8 46,855 + GFF3541 0.41 -1.6 46,855 + GFF3541 0.41 -0.8 46,855 + GFF3541 0.41 +0.2 46,855 + GFF3541 0.41 +1.0 46,855 + GFF3541 0.41 -0.0 46,855 + GFF3541 0.41 -1.0 46,855 + GFF3541 0.41 -0.6 46,855 + GFF3541 0.41 -1.0 46,855 + GFF3541 0.41 -0.6 46,855 + GFF3541 0.41 +0.1 46,856 - GFF3541 0.41 +0.8 46,856 - GFF3541 0.41 -0.3 46,856 - GFF3541 0.41 -0.4 46,856 - GFF3541 0.41 -0.4 46,856 - GFF3541 0.41 -0.2 46,856 - GFF3541 0.41 -0.7 46,856 - GFF3541 0.41 -0.0 46,856 - GFF3541 0.41 +0.0 46,856 - GFF3541 0.41 -0.2 46,927 + GFF3541 0.50 -0.1 47,102 - GFF3541 0.71 -0.5 47,125 + GFF3541 0.74 +0.4 47,125 + GFF3541 0.74 -0.0 47,125 + GFF3541 0.74 +0.4 47,125 + GFF3541 0.74 +0.8 47,126 - GFF3541 0.74 +0.6 47,126 - GFF3541 0.74 -0.4 47,354 + -1.5 47,354 + +0.6 47,354 + -0.3 47,355 - +0.2 47,355 - -0.3 47,365 - -1.0 47,365 - +0.0 47,365 - -1.1 47,592 + GFF3542 0.49 -0.5 47,592 + GFF3542 0.49 +0.1 47,592 + GFF3542 0.49 -1.1 47,592 + GFF3542 0.49 -0.3 47,592 + GFF3542 0.49 +0.8 47,592 + GFF3542 0.49 -0.8 47,592 + GFF3542 0.49 +0.0 47,592 + GFF3542 0.49 +0.1 47,592 + GFF3542 0.49 -0.2 47,592 + GFF3542 0.49 +1.8 47,592 + GFF3542 0.49 -0.2 47,592 + GFF3542 0.49 +0.6 47,592 + GFF3542 0.49 -0.5 47,593 - GFF3542 0.50 -0.0 47,593 - GFF3542 0.50 -0.6 47,593 - GFF3542 0.50 -0.8 47,593 - GFF3542 0.50 -0.1 47,593 - GFF3542 0.50 -1.0 47,593 - GFF3542 0.50 +0.6 47,595 + GFF3542 0.50 -0.6 47,595 + GFF3542 0.50 +0.2 47,595 + GFF3542 0.50 +0.5 47,595 + GFF3542 0.50 +0.2 47,595 + GFF3542 0.50 +0.2 47,595 + GFF3542 0.50 +0.2 47,595 + GFF3542 0.50 +0.4 47,595 + GFF3542 0.50 +0.7 47,595 + GFF3542 0.50 -0.9 47,596 - GFF3542 0.50 -0.8 47,596 - GFF3542 0.50 -0.1 47,596 - GFF3542 0.50 -0.1 47,596 - GFF3542 0.50 -0.1 47,596 - GFF3542 0.50 -0.3 47,596 - GFF3542 0.50 -0.2 47,596 - GFF3542 0.50 +0.2 47,596 - GFF3542 0.50 +0.2 47,596 - GFF3542 0.50 +0.1 47,619 + GFF3542 0.55 -2.3 47,619 + GFF3542 0.55 -0.4 47,619 + GFF3542 0.55 +0.1 47,619 + GFF3542 0.55 -0.3 47,619 + GFF3542 0.55 -1.3 47,620 - GFF3542 0.55 -0.9 47,620 - GFF3542 0.55 -0.8 47,620 - GFF3542 0.55 -0.0 47,894 + +0.4 47,894 + -0.2 47,894 + -0.2 47,894 + -0.4 47,895 - -0.7 47,895 - +1.6 47,909 + +0.1 47,909 + +0.7 47,910 - +0.5 47,927 + GFF3543 0.12 -0.4 47,927 + GFF3543 0.12 -0.0 47,928 - GFF3543 0.12 +0.4 47,928 - GFF3543 0.12 +1.0 47,928 - GFF3543 0.12 -1.0 48,049 + GFF3543 0.32 -0.6 48,049 + GFF3543 0.32 -0.9 48,049 + GFF3543 0.32 -0.6 48,049 + GFF3543 0.32 +0.4 48,049 + GFF3543 0.32 -0.1 48,049 + GFF3543 0.32 -1.1 48,049 + GFF3543 0.32 +0.8 48,049 + GFF3543 0.32 +0.7 48,050 - GFF3543 0.32 -0.9 48,050 - GFF3543 0.32 +0.3 48,050 - GFF3543 0.32 -1.4 48,050 - GFF3543 0.32 -0.7 48,050 - GFF3543 0.32 -0.2 48,050 - GFF3543 0.32 -0.7 48,050 - GFF3543 0.32 +0.1 48,050 - GFF3543 0.32 -0.4 48,050 - GFF3543 0.32 -0.1 48,050 - GFF3543 0.32 -0.3 48,331 + GFF3543 0.79 -0.2 48,331 + GFF3543 0.79 +0.4 48,331 + GFF3543 0.79 -0.5 48,331 + GFF3543 0.79 +0.3 48,332 - GFF3543 0.80 -1.1 48,332 - GFF3543 0.80 -0.1 48,332 - GFF3543 0.80 +0.2
Or see this region's nucleotide sequence