Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3525 and GFF3526 overlap by 4 nucleotides GFF3526 and GFF3527 overlap by 4 nucleotides GFF3527 and GFF3528 are separated by 73 nucleotides
GFF3525 - Protein tyrosine/serine phosphatase, at 31,581 to 32,117
GFF3525
GFF3526 - hypothetical protein, at 32,114 to 32,608
GFF3526
GFF3527 - UDP-N-acetylglucosamine:LPS N-acetylglucosamine transferase, at 32,605 to 33,750
GFF3527
GFF3528 - Nicotinamide phosphoribosyltransferase (EC 2.4.2.12), at 33,824 to 35,269
GFF3528
Position (kb) 32
33
34 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 31.654 kb on + strand, within GFF3525 at 31.684 kb on + strand, within GFF3525 at 31.684 kb on + strand, within GFF3525 at 31.753 kb on + strand, within GFF3525 at 31.754 kb on - strand, within GFF3525 at 31.754 kb on - strand, within GFF3525 at 32.409 kb on + strand, within GFF3526 at 32.410 kb on - strand, within GFF3526 at 32.416 kb on - strand, within GFF3526 at 32.563 kb on - strand at 32.733 kb on - strand, within GFF3527 at 32.733 kb on - strand, within GFF3527 at 32.733 kb on - strand, within GFF3527 at 32.733 kb on - strand, within GFF3527 at 32.990 kb on + strand, within GFF3527 at 32.991 kb on - strand, within GFF3527 at 32.991 kb on - strand, within GFF3527 at 32.991 kb on - strand, within GFF3527 at 33.329 kb on + strand, within GFF3527 at 33.330 kb on - strand, within GFF3527 at 33.545 kb on + strand, within GFF3527 at 33.546 kb on - strand, within GFF3527 at 33.546 kb on - strand, within GFF3527 at 33.584 kb on + strand, within GFF3527 at 33.584 kb on + strand, within GFF3527 at 33.584 kb on + strand, within GFF3527 at 33.585 kb on - strand, within GFF3527 at 33.585 kb on - strand, within GFF3527 at 33.585 kb on - strand, within GFF3527 at 33.585 kb on - strand, within GFF3527 at 33.585 kb on - strand, within GFF3527 at 33.585 kb on - strand, within GFF3527 at 33.585 kb on - strand, within GFF3527 at 33.585 kb on - strand, within GFF3527 at 33.585 kb on - strand, within GFF3527 at 33.585 kb on - strand, within GFF3527 at 33.596 kb on + strand, within GFF3527 at 33.596 kb on + strand, within GFF3527 at 33.773 kb on + strand at 33.773 kb on + strand at 33.774 kb on - strand at 33.825 kb on + strand at 33.825 kb on + strand at 33.825 kb on + strand at 34.062 kb on + strand, within GFF3528 at 34.063 kb on - strand, within GFF3528 at 34.063 kb on - strand, within GFF3528 at 34.452 kb on + strand, within GFF3528 at 34.452 kb on + strand, within GFF3528 at 34.452 kb on + strand, within GFF3528 at 34.452 kb on + strand, within GFF3528 at 34.452 kb on + strand, within GFF3528 at 34.452 kb on + strand, within GFF3528 at 34.452 kb on + strand, within GFF3528 at 34.452 kb on + strand, within GFF3528 at 34.453 kb on - strand, within GFF3528 at 34.453 kb on - strand, within GFF3528 at 34.453 kb on - strand, within GFF3528 at 34.453 kb on - strand, within GFF3528 at 34.453 kb on - strand, within GFF3528 at 34.453 kb on - strand, within GFF3528 at 34.453 kb on - strand, within GFF3528 at 34.515 kb on + strand, within GFF3528 at 34.516 kb on - strand, within GFF3528 at 34.603 kb on - strand, within GFF3528 at 34.603 kb on - strand, within GFF3528 at 34.603 kb on - strand, within GFF3528 at 34.603 kb on - strand, within GFF3528 at 34.605 kb on + strand, within GFF3528
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 31,654 + GFF3525 0.14 +0.0 31,684 + GFF3525 0.19 -0.6 31,684 + GFF3525 0.19 +0.8 31,753 + GFF3525 0.32 +2.0 31,754 - GFF3525 0.32 +0.0 31,754 - GFF3525 0.32 -0.4 32,409 + GFF3526 0.60 -0.5 32,410 - GFF3526 0.60 -0.2 32,416 - GFF3526 0.61 -0.2 32,563 - -2.7 32,733 - GFF3527 0.11 +0.2 32,733 - GFF3527 0.11 +0.0 32,733 - GFF3527 0.11 +0.1 32,733 - GFF3527 0.11 -1.5 32,990 + GFF3527 0.34 +0.0 32,991 - GFF3527 0.34 +0.5 32,991 - GFF3527 0.34 +0.5 32,991 - GFF3527 0.34 +1.8 33,329 + GFF3527 0.63 -0.3 33,330 - GFF3527 0.63 -1.2 33,545 + GFF3527 0.82 +0.1 33,546 - GFF3527 0.82 -1.7 33,546 - GFF3527 0.82 +0.1 33,584 + GFF3527 0.85 +2.8 33,584 + GFF3527 0.85 +0.5 33,584 + GFF3527 0.85 +0.5 33,585 - GFF3527 0.86 +0.2 33,585 - GFF3527 0.86 +0.2 33,585 - GFF3527 0.86 -0.3 33,585 - GFF3527 0.86 +0.5 33,585 - GFF3527 0.86 +1.8 33,585 - GFF3527 0.86 +1.2 33,585 - GFF3527 0.86 +0.2 33,585 - GFF3527 0.86 +0.2 33,585 - GFF3527 0.86 -0.6 33,585 - GFF3527 0.86 +0.8 33,596 + GFF3527 0.86 +0.8 33,596 + GFF3527 0.86 -0.5 33,773 + +0.2 33,773 + -0.4 33,774 - -1.2 33,825 + +1.0 33,825 + -1.6 33,825 + +0.3 34,062 + GFF3528 0.16 -0.1 34,063 - GFF3528 0.17 +0.1 34,063 - GFF3528 0.17 -1.4 34,452 + GFF3528 0.43 +0.1 34,452 + GFF3528 0.43 -0.1 34,452 + GFF3528 0.43 +0.5 34,452 + GFF3528 0.43 -1.8 34,452 + GFF3528 0.43 -0.8 34,452 + GFF3528 0.43 -0.4 34,452 + GFF3528 0.43 -0.9 34,452 + GFF3528 0.43 -0.3 34,453 - GFF3528 0.43 -1.2 34,453 - GFF3528 0.43 -0.7 34,453 - GFF3528 0.43 +0.4 34,453 - GFF3528 0.43 -0.1 34,453 - GFF3528 0.43 -0.4 34,453 - GFF3528 0.43 -2.2 34,453 - GFF3528 0.43 -0.6 34,515 + GFF3528 0.48 +0.1 34,516 - GFF3528 0.48 -0.6 34,603 - GFF3528 0.54 -0.7 34,603 - GFF3528 0.54 +0.4 34,603 - GFF3528 0.54 -0.3 34,603 - GFF3528 0.54 -0.7 34,605 + GFF3528 0.54 +0.8
Or see this region's nucleotide sequence