Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3496 and GFF3497 are separated by 117 nucleotides GFF3497 and GFF3498 overlap by 4 nucleotides GFF3498 and GFF3499 are separated by 24 nucleotides
GFF3496 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 1,052 to 2,974
GFF3496
GFF3497 - Transcriptional regulator, Crp/Fnr family, at 3,092 to 3,871
GFF3497
GFF3498 - hypothetical protein, at 3,868 to 4,335
GFF3498
GFF3499 - Toxin HigB / Protein kinase domain of HipA, at 4,360 to 5,580
GFF3499
Position (kb)
3
4 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2.178 kb on + strand, within GFF3496 at 2.178 kb on + strand, within GFF3496 at 2.179 kb on - strand, within GFF3496 at 2.179 kb on - strand, within GFF3496 at 2.181 kb on + strand, within GFF3496 at 2.310 kb on + strand, within GFF3496 at 2.311 kb on - strand, within GFF3496 at 2.311 kb on - strand, within GFF3496 at 2.430 kb on + strand, within GFF3496 at 2.431 kb on - strand, within GFF3496 at 2.481 kb on + strand, within GFF3496 at 2.481 kb on + strand, within GFF3496 at 2.481 kb on + strand, within GFF3496 at 2.481 kb on + strand, within GFF3496 at 2.481 kb on + strand, within GFF3496 at 2.482 kb on - strand, within GFF3496 at 2.482 kb on - strand, within GFF3496 at 2.482 kb on - strand, within GFF3496 at 2.485 kb on - strand, within GFF3496 at 2.535 kb on + strand, within GFF3496 at 2.535 kb on + strand, within GFF3496 at 2.536 kb on - strand, within GFF3496 at 2.536 kb on - strand, within GFF3496 at 2.560 kb on - strand, within GFF3496 at 2.560 kb on - strand, within GFF3496 at 2.586 kb on + strand, within GFF3496 at 2.586 kb on + strand, within GFF3496 at 2.587 kb on - strand, within GFF3496 at 2.736 kb on + strand, within GFF3496 at 2.736 kb on + strand, within GFF3496 at 2.787 kb on + strand at 2.787 kb on + strand at 2.787 kb on + strand at 2.787 kb on + strand at 2.788 kb on - strand at 2.788 kb on - strand at 2.788 kb on - strand at 2.788 kb on - strand at 2.788 kb on - strand at 2.788 kb on - strand at 2.788 kb on - strand at 3.021 kb on + strand at 3.021 kb on + strand at 3.021 kb on + strand at 3.021 kb on + strand at 3.021 kb on + strand at 3.022 kb on - strand at 3.022 kb on - strand at 3.022 kb on - strand at 3.072 kb on + strand at 3.073 kb on + strand at 3.073 kb on + strand at 3.073 kb on + strand at 3.073 kb on + strand at 3.073 kb on + strand at 3.073 kb on + strand at 3.074 kb on - strand at 3.074 kb on - strand at 3.074 kb on - strand at 3.074 kb on - strand at 3.296 kb on + strand, within GFF3497 at 3.296 kb on + strand, within GFF3497 at 3.296 kb on + strand, within GFF3497 at 3.296 kb on + strand, within GFF3497 at 3.297 kb on - strand, within GFF3497 at 3.297 kb on - strand, within GFF3497 at 3.297 kb on - strand, within GFF3497 at 3.297 kb on - strand, within GFF3497 at 3.297 kb on - strand, within GFF3497 at 3.297 kb on - strand, within GFF3497 at 3.365 kb on + strand, within GFF3497 at 3.365 kb on + strand, within GFF3497 at 3.365 kb on + strand, within GFF3497 at 3.365 kb on + strand, within GFF3497 at 3.366 kb on - strand, within GFF3497 at 3.366 kb on - strand, within GFF3497 at 3.366 kb on - strand, within GFF3497 at 3.431 kb on + strand, within GFF3497 at 3.432 kb on - strand, within GFF3497 at 3.432 kb on - strand, within GFF3497 at 3.647 kb on + strand, within GFF3497 at 3.647 kb on + strand, within GFF3497 at 3.647 kb on + strand, within GFF3497 at 3.695 kb on + strand, within GFF3497 at 3.695 kb on + strand, within GFF3497 at 3.696 kb on - strand, within GFF3497 at 3.696 kb on - strand, within GFF3497 at 3.773 kb on + strand, within GFF3497 at 3.773 kb on + strand, within GFF3497 at 3.774 kb on - strand, within GFF3497 at 3.833 kb on + strand at 4.349 kb on + strand at 4.349 kb on + strand at 4.349 kb on + strand at 4.349 kb on + strand at 4.349 kb on + strand at 4.349 kb on + strand at 4.350 kb on - strand at 4.352 kb on - strand at 4.361 kb on + strand at 4.376 kb on + strand at 4.376 kb on + strand at 4.376 kb on + strand at 4.376 kb on + strand at 4.376 kb on + strand at 4.376 kb on + strand at 4.377 kb on - strand at 4.377 kb on - strand at 4.377 kb on - strand at 4.377 kb on - strand at 4.377 kb on - strand at 4.377 kb on - strand at 4.377 kb on - strand at 4.377 kb on - strand at 4.519 kb on + strand, within GFF3499 at 4.520 kb on - strand, within GFF3499 at 4.520 kb on - strand, within GFF3499 at 4.634 kb on + strand, within GFF3499 at 4.634 kb on + strand, within GFF3499 at 4.635 kb on - strand, within GFF3499 at 4.643 kb on + strand, within GFF3499 at 4.644 kb on - strand, within GFF3499 at 4.649 kb on + strand, within GFF3499 at 4.649 kb on + strand, within GFF3499 at 4.649 kb on + strand, within GFF3499 at 4.649 kb on + strand, within GFF3499 at 4.649 kb on + strand, within GFF3499 at 4.649 kb on + strand, within GFF3499 at 4.649 kb on + strand, within GFF3499 at 4.649 kb on + strand, within GFF3499 at 4.649 kb on + strand, within GFF3499 at 4.650 kb on - strand, within GFF3499 at 4.650 kb on - strand, within GFF3499 at 4.709 kb on + strand, within GFF3499 at 4.710 kb on - strand, within GFF3499 at 4.710 kb on - strand, within GFF3499 at 4.710 kb on - strand, within GFF3499 at 4.710 kb on - strand, within GFF3499 at 4.710 kb on - strand, within GFF3499 at 4.721 kb on + strand, within GFF3499 at 4.722 kb on - strand, within GFF3499 at 4.722 kb on - strand, within GFF3499 at 4.722 kb on - strand, within GFF3499 at 4.722 kb on - strand, within GFF3499 at 4.814 kb on + strand, within GFF3499 at 4.814 kb on + strand, within GFF3499 at 4.814 kb on + strand, within GFF3499 at 4.815 kb on - strand, within GFF3499 at 4.815 kb on - strand, within GFF3499 at 4.815 kb on - strand, within GFF3499 at 4.841 kb on + strand, within GFF3499 at 4.841 kb on + strand, within GFF3499 at 4.841 kb on + strand, within GFF3499 at 4.853 kb on + strand, within GFF3499 at 4.854 kb on - strand, within GFF3499 at 4.854 kb on - strand, within GFF3499 at 4.854 kb on - strand, within GFF3499 at 4.854 kb on - strand, within GFF3499
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 2,178 + GFF3496 0.59 +0.2 2,178 + GFF3496 0.59 +0.4 2,179 - GFF3496 0.59 +0.0 2,179 - GFF3496 0.59 +0.4 2,181 + GFF3496 0.59 -0.3 2,310 + GFF3496 0.65 -0.4 2,311 - GFF3496 0.65 +0.3 2,311 - GFF3496 0.65 +0.8 2,430 + GFF3496 0.72 -0.8 2,431 - GFF3496 0.72 -0.3 2,481 + GFF3496 0.74 -0.3 2,481 + GFF3496 0.74 -0.8 2,481 + GFF3496 0.74 +0.5 2,481 + GFF3496 0.74 -0.2 2,481 + GFF3496 0.74 -0.4 2,482 - GFF3496 0.74 -0.1 2,482 - GFF3496 0.74 +0.3 2,482 - GFF3496 0.74 -0.3 2,485 - GFF3496 0.75 -0.1 2,535 + GFF3496 0.77 -0.5 2,535 + GFF3496 0.77 -0.4 2,536 - GFF3496 0.77 -0.1 2,536 - GFF3496 0.77 -0.1 2,560 - GFF3496 0.78 -0.5 2,560 - GFF3496 0.78 -1.2 2,586 + GFF3496 0.80 +2.2 2,586 + GFF3496 0.80 +0.8 2,587 - GFF3496 0.80 -0.2 2,736 + GFF3496 0.88 +1.2 2,736 + GFF3496 0.88 +0.5 2,787 + +0.1 2,787 + +2.2 2,787 + -0.1 2,787 + +0.2 2,788 - -0.1 2,788 - -1.0 2,788 - +0.1 2,788 - +0.4 2,788 - -0.5 2,788 - -2.0 2,788 - +0.8 3,021 + +0.2 3,021 + -0.7 3,021 + +0.2 3,021 + -0.1 3,021 + -1.0 3,022 - -2.5 3,022 - -0.4 3,022 - -0.3 3,072 + -0.3 3,073 + -0.2 3,073 + -0.2 3,073 + -0.1 3,073 + -1.2 3,073 + +0.8 3,073 + +0.2 3,074 - +0.8 3,074 - +1.5 3,074 - -1.2 3,074 - +0.5 3,296 + GFF3497 0.26 -0.6 3,296 + GFF3497 0.26 -0.4 3,296 + GFF3497 0.26 -0.8 3,296 + GFF3497 0.26 -0.7 3,297 - GFF3497 0.26 +0.3 3,297 - GFF3497 0.26 -0.6 3,297 - GFF3497 0.26 -1.0 3,297 - GFF3497 0.26 -0.8 3,297 - GFF3497 0.26 -0.3 3,297 - GFF3497 0.26 +0.4 3,365 + GFF3497 0.35 -0.2 3,365 + GFF3497 0.35 -0.0 3,365 + GFF3497 0.35 -0.6 3,365 + GFF3497 0.35 -0.8 3,366 - GFF3497 0.35 -0.3 3,366 - GFF3497 0.35 -0.8 3,366 - GFF3497 0.35 -0.4 3,431 + GFF3497 0.43 +0.8 3,432 - GFF3497 0.44 +0.3 3,432 - GFF3497 0.44 -0.2 3,647 + GFF3497 0.71 -0.7 3,647 + GFF3497 0.71 -0.5 3,647 + GFF3497 0.71 -0.8 3,695 + GFF3497 0.77 +0.4 3,695 + GFF3497 0.77 +0.1 3,696 - GFF3497 0.77 -0.6 3,696 - GFF3497 0.77 -1.4 3,773 + GFF3497 0.87 -0.4 3,773 + GFF3497 0.87 -0.9 3,774 - GFF3497 0.87 -0.7 3,833 + -0.9 4,349 + -0.7 4,349 + -0.3 4,349 + +1.1 4,349 + +0.0 4,349 + -0.4 4,349 + -0.5 4,350 - -1.0 4,352 - -0.2 4,361 + -0.7 4,376 + -0.3 4,376 + -0.6 4,376 + -0.1 4,376 + +0.3 4,376 + +0.1 4,376 + -0.3 4,377 - -0.7 4,377 - +0.5 4,377 - -0.5 4,377 - -0.1 4,377 - +0.3 4,377 - +0.1 4,377 - -0.3 4,377 - +0.1 4,519 + GFF3499 0.13 -0.8 4,520 - GFF3499 0.13 -0.1 4,520 - GFF3499 0.13 -0.6 4,634 + GFF3499 0.22 -1.0 4,634 + GFF3499 0.22 -2.2 4,635 - GFF3499 0.23 +0.4 4,643 + GFF3499 0.23 -0.3 4,644 - GFF3499 0.23 -0.8 4,649 + GFF3499 0.24 +0.7 4,649 + GFF3499 0.24 -2.4 4,649 + GFF3499 0.24 -0.7 4,649 + GFF3499 0.24 +1.8 4,649 + GFF3499 0.24 +0.3 4,649 + GFF3499 0.24 +0.2 4,649 + GFF3499 0.24 +0.2 4,649 + GFF3499 0.24 +0.5 4,649 + GFF3499 0.24 -1.0 4,650 - GFF3499 0.24 -0.8 4,650 - GFF3499 0.24 -1.2 4,709 + GFF3499 0.29 -1.0 4,710 - GFF3499 0.29 +0.8 4,710 - GFF3499 0.29 +0.8 4,710 - GFF3499 0.29 -0.8 4,710 - GFF3499 0.29 +0.1 4,710 - GFF3499 0.29 -0.1 4,721 + GFF3499 0.30 -0.6 4,722 - GFF3499 0.30 -0.8 4,722 - GFF3499 0.30 -0.7 4,722 - GFF3499 0.30 -0.0 4,722 - GFF3499 0.30 +0.4 4,814 + GFF3499 0.37 -0.3 4,814 + GFF3499 0.37 +0.9 4,814 + GFF3499 0.37 -0.1 4,815 - GFF3499 0.37 +0.7 4,815 - GFF3499 0.37 +0.4 4,815 - GFF3499 0.37 -1.9 4,841 + GFF3499 0.39 +0.5 4,841 + GFF3499 0.39 -0.1 4,841 + GFF3499 0.39 -0.0 4,853 + GFF3499 0.40 -0.4 4,854 - GFF3499 0.40 +0.1 4,854 - GFF3499 0.40 +0.1 4,854 - GFF3499 0.40 +0.8 4,854 - GFF3499 0.40 +1.8
Or see this region's nucleotide sequence