Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3480 and GFF3481 overlap by 8 nucleotides GFF3481 and GFF3482 overlap by 4 nucleotides GFF3482 and GFF3483 overlap by 4 nucleotides GFF3483 and GFF3484 are separated by 67 nucleotides
GFF3480 - ABC transporter, ATP-binding protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 303,126 to 303,830
GFF3480
GFF3481 - ABC transporter, ATP-binding protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 303,823 to 304,593
GFF3481
GFF3482 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 304,590 to 305,552
GFF3482
GFF3483 - ABC transporter, permease protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 305,549 to 306,409
GFF3483
GFF3484 - ABC transporter, substrate-binding protein (cluster 4, leucine/isoleucine/valine/benzoate), at 306,477 to 307,670
GFF3484
Position (kb) 304
305
306 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 303.851 kb on + strand at 303.851 kb on + strand at 303.851 kb on + strand at 303.852 kb on - strand at 303.852 kb on - strand at 303.852 kb on - strand at 303.852 kb on - strand at 303.852 kb on - strand at 303.852 kb on - strand at 304.177 kb on + strand, within GFF3481 at 304.177 kb on + strand, within GFF3481 at 304.177 kb on + strand, within GFF3481 at 304.178 kb on - strand, within GFF3481 at 304.178 kb on - strand, within GFF3481 at 304.692 kb on - strand, within GFF3482 at 304.697 kb on - strand, within GFF3482 at 304.697 kb on - strand, within GFF3482 at 304.738 kb on + strand, within GFF3482 at 304.738 kb on + strand, within GFF3482 at 304.739 kb on - strand, within GFF3482 at 304.739 kb on - strand, within GFF3482 at 304.739 kb on - strand, within GFF3482 at 304.739 kb on - strand, within GFF3482 at 304.739 kb on - strand, within GFF3482 at 304.739 kb on - strand, within GFF3482 at 304.906 kb on + strand, within GFF3482 at 304.907 kb on - strand, within GFF3482 at 304.907 kb on - strand, within GFF3482 at 304.907 kb on - strand, within GFF3482 at 304.907 kb on - strand, within GFF3482 at 305.053 kb on + strand, within GFF3482 at 305.240 kb on - strand, within GFF3482 at 305.240 kb on - strand, within GFF3482 at 305.240 kb on - strand, within GFF3482 at 305.251 kb on + strand, within GFF3482 at 305.252 kb on - strand, within GFF3482 at 305.314 kb on + strand, within GFF3482 at 305.314 kb on + strand, within GFF3482 at 305.314 kb on + strand, within GFF3482 at 305.315 kb on - strand, within GFF3482 at 305.315 kb on - strand, within GFF3482 at 305.339 kb on - strand, within GFF3482 at 305.389 kb on + strand, within GFF3482 at 305.616 kb on + strand at 305.616 kb on + strand at 305.616 kb on + strand at 305.616 kb on + strand at 305.616 kb on + strand at 305.617 kb on - strand at 305.617 kb on - strand at 305.617 kb on - strand at 305.617 kb on - strand at 305.617 kb on - strand at 305.617 kb on - strand at 305.617 kb on - strand at 305.617 kb on - strand at 305.617 kb on - strand at 305.646 kb on + strand, within GFF3483 at 305.646 kb on + strand, within GFF3483 at 305.646 kb on + strand, within GFF3483 at 305.646 kb on + strand, within GFF3483 at 305.646 kb on + strand, within GFF3483 at 305.647 kb on - strand, within GFF3483 at 305.649 kb on + strand, within GFF3483 at 305.649 kb on + strand, within GFF3483 at 305.649 kb on + strand, within GFF3483 at 305.649 kb on + strand, within GFF3483 at 305.650 kb on - strand, within GFF3483 at 305.650 kb on - strand, within GFF3483 at 305.650 kb on - strand, within GFF3483 at 305.650 kb on - strand, within GFF3483 at 305.650 kb on - strand, within GFF3483 at 305.650 kb on - strand, within GFF3483 at 305.650 kb on - strand, within GFF3483 at 305.745 kb on + strand, within GFF3483 at 305.754 kb on + strand, within GFF3483 at 305.754 kb on + strand, within GFF3483 at 305.754 kb on + strand, within GFF3483 at 305.755 kb on - strand, within GFF3483 at 305.755 kb on - strand, within GFF3483 at 305.755 kb on - strand, within GFF3483 at 306.020 kb on + strand, within GFF3483 at 306.084 kb on + strand, within GFF3483 at 306.084 kb on + strand, within GFF3483 at 306.085 kb on - strand, within GFF3483 at 306.156 kb on + strand, within GFF3483 at 306.157 kb on - strand, within GFF3483 at 306.169 kb on - strand, within GFF3483 at 306.277 kb on - strand, within GFF3483 at 306.277 kb on - strand, within GFF3483 at 306.378 kb on + strand at 306.379 kb on - strand at 306.379 kb on - strand at 306.478 kb on + strand at 306.478 kb on + strand at 306.478 kb on + strand at 306.478 kb on + strand at 306.478 kb on + strand at 306.478 kb on + strand at 306.478 kb on + strand at 306.478 kb on + strand at 306.478 kb on + strand at 306.479 kb on - strand at 306.479 kb on - strand at 306.479 kb on - strand at 306.479 kb on - strand at 306.479 kb on - strand at 306.479 kb on - strand at 306.479 kb on - strand at 306.479 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 303,851 + +1.0 303,851 + -0.1 303,851 + -0.3 303,852 - -0.2 303,852 - -0.3 303,852 - -1.1 303,852 - -1.5 303,852 - -0.8 303,852 - -0.8 304,177 + GFF3481 0.46 +0.1 304,177 + GFF3481 0.46 -0.6 304,177 + GFF3481 0.46 -0.4 304,178 - GFF3481 0.46 +0.3 304,178 - GFF3481 0.46 +0.0 304,692 - GFF3482 0.11 -2.3 304,697 - GFF3482 0.11 -0.1 304,697 - GFF3482 0.11 -1.9 304,738 + GFF3482 0.15 +3.4 304,738 + GFF3482 0.15 -0.6 304,739 - GFF3482 0.15 -0.3 304,739 - GFF3482 0.15 -0.8 304,739 - GFF3482 0.15 -0.2 304,739 - GFF3482 0.15 -0.4 304,739 - GFF3482 0.15 +1.3 304,739 - GFF3482 0.15 -0.6 304,906 + GFF3482 0.33 -0.1 304,907 - GFF3482 0.33 +0.1 304,907 - GFF3482 0.33 -1.8 304,907 - GFF3482 0.33 +0.9 304,907 - GFF3482 0.33 -2.0 305,053 + GFF3482 0.48 -0.2 305,240 - GFF3482 0.67 +1.5 305,240 - GFF3482 0.67 -1.1 305,240 - GFF3482 0.67 -0.5 305,251 + GFF3482 0.69 +1.2 305,252 - GFF3482 0.69 +1.0 305,314 + GFF3482 0.75 -0.1 305,314 + GFF3482 0.75 -0.8 305,314 + GFF3482 0.75 +0.2 305,315 - GFF3482 0.75 -0.8 305,315 - GFF3482 0.75 +3.1 305,339 - GFF3482 0.78 -0.1 305,389 + GFF3482 0.83 -1.2 305,616 + -0.2 305,616 + -1.0 305,616 + -0.2 305,616 + -0.9 305,616 + +1.0 305,617 - +0.8 305,617 - -0.0 305,617 - -0.8 305,617 - +0.0 305,617 - -1.3 305,617 - -0.5 305,617 - +0.1 305,617 - -1.4 305,617 - +0.6 305,646 + GFF3483 0.11 -0.1 305,646 + GFF3483 0.11 +0.3 305,646 + GFF3483 0.11 +0.1 305,646 + GFF3483 0.11 +0.3 305,646 + GFF3483 0.11 +1.2 305,647 - GFF3483 0.11 +0.4 305,649 + GFF3483 0.12 +0.1 305,649 + GFF3483 0.12 +1.0 305,649 + GFF3483 0.12 +0.4 305,649 + GFF3483 0.12 +0.2 305,650 - GFF3483 0.12 +0.7 305,650 - GFF3483 0.12 +0.3 305,650 - GFF3483 0.12 +1.3 305,650 - GFF3483 0.12 +2.6 305,650 - GFF3483 0.12 +0.0 305,650 - GFF3483 0.12 +1.2 305,650 - GFF3483 0.12 +0.8 305,745 + GFF3483 0.23 -0.2 305,754 + GFF3483 0.24 +1.3 305,754 + GFF3483 0.24 -0.8 305,754 + GFF3483 0.24 -0.5 305,755 - GFF3483 0.24 -2.2 305,755 - GFF3483 0.24 +0.6 305,755 - GFF3483 0.24 +0.0 306,020 + GFF3483 0.55 -0.3 306,084 + GFF3483 0.62 -1.4 306,084 + GFF3483 0.62 +0.3 306,085 - GFF3483 0.62 -1.0 306,156 + GFF3483 0.70 -0.7 306,157 - GFF3483 0.71 -0.4 306,169 - GFF3483 0.72 -0.4 306,277 - GFF3483 0.85 -0.4 306,277 - GFF3483 0.85 -0.8 306,378 + +0.8 306,379 - +1.1 306,379 - -0.6 306,478 + -0.3 306,478 + +1.2 306,478 + -0.7 306,478 + -0.9 306,478 + -0.4 306,478 + -0.3 306,478 + -1.9 306,478 + -1.3 306,478 + -0.7 306,479 - +0.1 306,479 - -1.0 306,479 - -1.4 306,479 - -0.2 306,479 - -0.5 306,479 - -0.2 306,479 - -0.4 306,479 - +1.0
Or see this region's nucleotide sequence