Strain Fitness in Variovorax sp. SCN45 around GFF3421

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3419 and GFF3420 overlap by 4 nucleotidesGFF3420 and GFF3421 are separated by 100 nucleotidesGFF3421 and GFF3422 are separated by 31 nucleotidesGFF3422 and GFF3423 are separated by 8 nucleotides GFF3419 - ABC transporter, substrate-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 242,576 to 243,535 GFF3419 GFF3420 - Transcriptional regulator, AcrR family, at 243,532 to 244,284 GFF3420 GFF3421 - Acetoacetyl-CoA synthetase (EC 6.2.1.16), at 244,385 to 246,388 GFF3421 GFF3422 - Lipoprotein-related protein, at 246,420 to 246,842 GFF3422 GFF3423 - Adenylate cyclase (EC 4.6.1.1), at 246,851 to 248,785 GFF3423 Position (kb) 244 245 246 247Strain fitness (log2 ratio) -2 -1 0 1 2at 243.797 kb on + strand, within GFF3420at 243.798 kb on - strand, within GFF3420at 243.798 kb on - strand, within GFF3420at 243.798 kb on - strand, within GFF3420at 243.821 kb on + strand, within GFF3420at 243.860 kb on + strand, within GFF3420at 243.860 kb on + strand, within GFF3420at 243.860 kb on + strand, within GFF3420at 243.887 kb on + strand, within GFF3420at 243.888 kb on - strand, within GFF3420at 243.891 kb on - strand, within GFF3420at 244.070 kb on + strand, within GFF3420at 244.070 kb on + strand, within GFF3420at 244.071 kb on - strand, within GFF3420at 244.073 kb on + strand, within GFF3420at 244.073 kb on + strand, within GFF3420at 244.073 kb on + strand, within GFF3420at 244.073 kb on + strand, within GFF3420at 244.073 kb on + strand, within GFF3420at 244.073 kb on + strand, within GFF3420at 244.073 kb on + strand, within GFF3420at 244.074 kb on - strand, within GFF3420at 244.074 kb on - strand, within GFF3420at 244.074 kb on - strand, within GFF3420at 244.074 kb on - strand, within GFF3420at 244.074 kb on - strand, within GFF3420at 244.074 kb on - strand, within GFF3420at 244.074 kb on - strand, within GFF3420at 244.074 kb on - strand, within GFF3420at 244.076 kb on + strand, within GFF3420at 244.077 kb on - strand, within GFF3420at 244.373 kb on + strandat 244.374 kb on - strandat 244.384 kb on + strandat 244.384 kb on + strandat 244.384 kb on + strandat 244.384 kb on + strandat 244.418 kb on + strandat 244.419 kb on - strandat 244.463 kb on + strandat 244.463 kb on + strandat 244.464 kb on - strandat 244.464 kb on - strandat 244.464 kb on - strandat 244.464 kb on - strandat 244.526 kb on + strandat 244.526 kb on + strandat 244.526 kb on + strandat 244.526 kb on + strandat 244.527 kb on - strandat 244.628 kb on + strand, within GFF3421at 244.629 kb on - strand, within GFF3421at 244.629 kb on - strand, within GFF3421at 244.811 kb on + strand, within GFF3421at 244.811 kb on + strand, within GFF3421at 244.811 kb on + strand, within GFF3421at 244.811 kb on + strand, within GFF3421at 244.812 kb on - strand, within GFF3421at 244.812 kb on - strand, within GFF3421at 244.812 kb on - strand, within GFF3421at 244.812 kb on - strand, within GFF3421at 244.812 kb on - strand, within GFF3421at 244.812 kb on - strand, within GFF3421at 244.970 kb on + strand, within GFF3421at 245.183 kb on + strand, within GFF3421at 245.315 kb on + strand, within GFF3421at 245.315 kb on + strand, within GFF3421at 245.325 kb on - strand, within GFF3421at 245.325 kb on - strand, within GFF3421at 245.325 kb on - strand, within GFF3421at 245.325 kb on - strand, within GFF3421at 245.325 kb on - strand, within GFF3421at 245.325 kb on - strand, within GFF3421at 245.325 kb on - strand, within GFF3421at 245.396 kb on + strand, within GFF3421at 245.397 kb on - strand, within GFF3421at 245.442 kb on + strand, within GFF3421at 245.442 kb on + strand, within GFF3421at 245.442 kb on + strand, within GFF3421at 245.442 kb on + strand, within GFF3421at 245.442 kb on + strand, within GFF3421at 245.442 kb on + strand, within GFF3421at 245.443 kb on - strand, within GFF3421at 245.443 kb on - strand, within GFF3421at 245.499 kb on - strand, within GFF3421at 245.850 kb on - strand, within GFF3421at 245.850 kb on - strand, within GFF3421at 245.850 kb on - strand, within GFF3421at 245.891 kb on + strand, within GFF3421at 245.892 kb on - strand, within GFF3421at 245.966 kb on + strand, within GFF3421at 245.966 kb on + strand, within GFF3421at 245.967 kb on - strand, within GFF3421at 245.967 kb on - strand, within GFF3421at 245.967 kb on - strand, within GFF3421at 246.026 kb on + strand, within GFF3421at 246.026 kb on + strand, within GFF3421at 246.027 kb on - strand, within GFF3421at 246.027 kb on - strand, within GFF3421at 246.027 kb on - strand, within GFF3421at 246.027 kb on - strand, within GFF3421at 246.027 kb on - strand, within GFF3421at 246.027 kb on - strand, within GFF3421at 246.027 kb on - strand, within GFF3421at 246.027 kb on - strand, within GFF3421at 246.027 kb on - strand, within GFF3421at 246.027 kb on - strand, within GFF3421at 246.083 kb on + strand, within GFF3421at 246.101 kb on + strand, within GFF3421at 246.101 kb on + strand, within GFF3421at 246.101 kb on + strand, within GFF3421at 246.101 kb on + strand, within GFF3421at 246.101 kb on + strand, within GFF3421at 246.101 kb on + strand, within GFF3421at 246.102 kb on - strand, within GFF3421at 246.102 kb on - strand, within GFF3421at 246.102 kb on - strand, within GFF3421at 246.102 kb on - strand, within GFF3421at 246.102 kb on - strand, within GFF3421at 246.102 kb on - strand, within GFF3421at 246.102 kb on - strand, within GFF3421at 246.353 kb on + strandat 246.353 kb on + strandat 246.353 kb on + strandat 246.353 kb on + strandat 246.354 kb on - strandat 246.354 kb on - strandat 246.354 kb on - strandat 246.354 kb on - strandat 246.354 kb on - strandat 246.354 kb on - strandat 246.354 kb on - strandat 246.354 kb on - strandat 246.354 kb on - strandat 246.386 kb on + strandat 246.407 kb on + strandat 246.408 kb on - strandat 246.664 kb on - strand, within GFF3422at 246.709 kb on - strand, within GFF3422at 246.709 kb on - strand, within GFF3422at 246.709 kb on - strand, within GFF3422at 246.768 kb on + strand, within GFF3422at 246.768 kb on + strand, within GFF3422at 246.870 kb on + strandat 246.870 kb on + strandat 247.047 kb on + strand, within GFF3423at 247.048 kb on - strand, within GFF3423at 247.048 kb on - strand, within GFF3423at 247.203 kb on + strand, within GFF3423at 247.203 kb on + strand, within GFF3423at 247.203 kb on + strand, within GFF3423at 247.204 kb on - strand, within GFF3423at 247.204 kb on - strand, within GFF3423at 247.317 kb on + strand, within GFF3423at 247.317 kb on + strand, within GFF3423at 247.317 kb on + strand, within GFF3423at 247.318 kb on - strand, within GFF3423

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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243,797 + GFF3420 0.35 -0.7
243,798 - GFF3420 0.35 -1.2
243,798 - GFF3420 0.35 -1.6
243,798 - GFF3420 0.35 -1.3
243,821 + GFF3420 0.38 +0.2
243,860 + GFF3420 0.44 -0.5
243,860 + GFF3420 0.44 -0.6
243,860 + GFF3420 0.44 +1.0
243,887 + GFF3420 0.47 -1.3
243,888 - GFF3420 0.47 -1.0
243,891 - GFF3420 0.48 -0.4
244,070 + GFF3420 0.71 -0.6
244,070 + GFF3420 0.71 -0.1
244,071 - GFF3420 0.72 +0.2
244,073 + GFF3420 0.72 -0.6
244,073 + GFF3420 0.72 -1.0
244,073 + GFF3420 0.72 -0.1
244,073 + GFF3420 0.72 +0.2
244,073 + GFF3420 0.72 -1.3
244,073 + GFF3420 0.72 -0.4
244,073 + GFF3420 0.72 -0.9
244,074 - GFF3420 0.72 -0.1
244,074 - GFF3420 0.72 -0.6
244,074 - GFF3420 0.72 +1.2
244,074 - GFF3420 0.72 +0.2
244,074 - GFF3420 0.72 -0.4
244,074 - GFF3420 0.72 -0.3
244,074 - GFF3420 0.72 -0.3
244,074 - GFF3420 0.72 +1.6
244,076 + GFF3420 0.72 -0.2
244,077 - GFF3420 0.72 -0.2
244,373 + +0.8
244,374 - -0.6
244,384 + +0.8
244,384 + -1.7
244,384 + +0.0
244,384 + +0.9
244,418 + +0.6
244,419 - +1.8
244,463 + -0.5
244,463 + -0.5
244,464 - +0.0
244,464 - +0.5
244,464 - -0.8
244,464 - +0.4
244,526 + +0.2
244,526 + +1.7
244,526 + +0.1
244,526 + -0.4
244,527 - -0.7
244,628 + GFF3421 0.12 -0.1
244,629 - GFF3421 0.12 +0.6
244,629 - GFF3421 0.12 -0.2
244,811 + GFF3421 0.21 -1.0
244,811 + GFF3421 0.21 -1.2
244,811 + GFF3421 0.21 +0.2
244,811 + GFF3421 0.21 +0.3
244,812 - GFF3421 0.21 -0.4
244,812 - GFF3421 0.21 -0.4
244,812 - GFF3421 0.21 -0.6
244,812 - GFF3421 0.21 -0.7
244,812 - GFF3421 0.21 -0.4
244,812 - GFF3421 0.21 +0.1
244,970 + GFF3421 0.29 +1.3
245,183 + GFF3421 0.40 +0.5
245,315 + GFF3421 0.46 -0.6
245,315 + GFF3421 0.46 +0.7
245,325 - GFF3421 0.47 -1.1
245,325 - GFF3421 0.47 -1.2
245,325 - GFF3421 0.47 +0.9
245,325 - GFF3421 0.47 -0.9
245,325 - GFF3421 0.47 +0.0
245,325 - GFF3421 0.47 -0.6
245,325 - GFF3421 0.47 +0.7
245,396 + GFF3421 0.50 +0.2
245,397 - GFF3421 0.50 +0.8
245,442 + GFF3421 0.53 +0.3
245,442 + GFF3421 0.53 -1.7
245,442 + GFF3421 0.53 -1.6
245,442 + GFF3421 0.53 +1.5
245,442 + GFF3421 0.53 +0.1
245,442 + GFF3421 0.53 +0.2
245,443 - GFF3421 0.53 -0.8
245,443 - GFF3421 0.53 -1.1
245,499 - GFF3421 0.56 +0.4
245,850 - GFF3421 0.73 +0.4
245,850 - GFF3421 0.73 +0.3
245,850 - GFF3421 0.73 +0.8
245,891 + GFF3421 0.75 -0.0
245,892 - GFF3421 0.75 -0.4
245,966 + GFF3421 0.79 +0.7
245,966 + GFF3421 0.79 -1.2
245,967 - GFF3421 0.79 -0.1
245,967 - GFF3421 0.79 -0.4
245,967 - GFF3421 0.79 -1.2
246,026 + GFF3421 0.82 +0.9
246,026 + GFF3421 0.82 +0.2
246,027 - GFF3421 0.82 +0.8
246,027 - GFF3421 0.82 +0.5
246,027 - GFF3421 0.82 +1.2
246,027 - GFF3421 0.82 +0.3
246,027 - GFF3421 0.82 +0.1
246,027 - GFF3421 0.82 -1.1
246,027 - GFF3421 0.82 +0.1
246,027 - GFF3421 0.82 +0.1
246,027 - GFF3421 0.82 -0.4
246,027 - GFF3421 0.82 +0.2
246,083 + GFF3421 0.85 +1.2
246,101 + GFF3421 0.86 +0.2
246,101 + GFF3421 0.86 +0.4
246,101 + GFF3421 0.86 -1.5
246,101 + GFF3421 0.86 -0.5
246,101 + GFF3421 0.86 +0.3
246,101 + GFF3421 0.86 -0.0
246,102 - GFF3421 0.86 +0.5
246,102 - GFF3421 0.86 +1.4
246,102 - GFF3421 0.86 -2.1
246,102 - GFF3421 0.86 -1.0
246,102 - GFF3421 0.86 +0.4
246,102 - GFF3421 0.86 -1.0
246,102 - GFF3421 0.86 -0.1
246,353 + -0.5
246,353 + -1.4
246,353 + -0.3
246,353 + +0.2
246,354 - +0.2
246,354 - -0.4
246,354 - -0.3
246,354 - -0.2
246,354 - -0.5
246,354 - -0.7
246,354 - -0.2
246,354 - -0.5
246,354 - -0.3
246,386 + -0.1
246,407 + -0.6
246,408 - -0.4
246,664 - GFF3422 0.58 +0.2
246,709 - GFF3422 0.68 -0.9
246,709 - GFF3422 0.68 +0.3
246,709 - GFF3422 0.68 -1.0
246,768 + GFF3422 0.82 -0.9
246,768 + GFF3422 0.82 -1.2
246,870 + +0.2
246,870 + -0.5
247,047 + GFF3423 0.10 -0.6
247,048 - GFF3423 0.10 -0.2
247,048 - GFF3423 0.10 +0.2
247,203 + GFF3423 0.18 +0.2
247,203 + GFF3423 0.18 +0.2
247,203 + GFF3423 0.18 +0.8
247,204 - GFF3423 0.18 +2.1
247,204 - GFF3423 0.18 +0.1
247,317 + GFF3423 0.24 +0.5
247,317 + GFF3423 0.24 -2.0
247,317 + GFF3423 0.24 -0.4
247,318 - GFF3423 0.24 -0.3

Or see this region's nucleotide sequence