Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3308 and GFF3309 overlap by 4 nucleotides GFF3309 and GFF3310 are separated by 20 nucleotides
GFF3308 - Inner membrane protein CreD, at 132,827 to 134,290
GFF3308
GFF3309 - Beta-hexosaminidase (EC 3.2.1.52), at 134,287 to 135,579
GFF3309
GFF3310 - Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-), at 135,600 to 138,002
GFF3310
Position (kb)
134
135
136 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 133.839 kb on - strand, within GFF3308 at 133.865 kb on + strand, within GFF3308 at 133.865 kb on + strand, within GFF3308 at 133.865 kb on + strand, within GFF3308 at 133.865 kb on + strand, within GFF3308 at 133.866 kb on - strand, within GFF3308 at 133.866 kb on - strand, within GFF3308 at 133.866 kb on - strand, within GFF3308 at 133.949 kb on + strand, within GFF3308 at 133.949 kb on + strand, within GFF3308 at 134.031 kb on - strand, within GFF3308 at 134.072 kb on + strand, within GFF3308 at 134.073 kb on - strand, within GFF3308 at 134.138 kb on + strand, within GFF3308 at 134.138 kb on + strand, within GFF3308 at 134.138 kb on + strand, within GFF3308 at 134.138 kb on + strand, within GFF3308 at 134.138 kb on + strand, within GFF3308 at 134.139 kb on - strand, within GFF3308 at 134.139 kb on - strand, within GFF3308 at 134.150 kb on + strand at 134.151 kb on - strand at 134.151 kb on - strand at 134.151 kb on - strand at 134.151 kb on - strand at 134.253 kb on - strand at 134.253 kb on - strand at 134.253 kb on - strand at 134.269 kb on + strand at 134.611 kb on + strand, within GFF3309 at 134.612 kb on - strand, within GFF3309 at 134.899 kb on + strand, within GFF3309 at 134.933 kb on + strand, within GFF3309 at 134.933 kb on + strand, within GFF3309 at 134.933 kb on + strand, within GFF3309 at 134.934 kb on - strand, within GFF3309 at 134.934 kb on - strand, within GFF3309 at 135.060 kb on - strand, within GFF3309 at 135.346 kb on + strand, within GFF3309 at 135.346 kb on + strand, within GFF3309 at 135.347 kb on - strand, within GFF3309 at 135.391 kb on + strand, within GFF3309 at 135.391 kb on + strand, within GFF3309 at 135.392 kb on - strand, within GFF3309 at 135.392 kb on - strand, within GFF3309 at 135.460 kb on + strand at 135.461 kb on - strand at 135.461 kb on - strand at 135.461 kb on - strand at 135.461 kb on - strand at 135.475 kb on + strand at 135.475 kb on + strand at 135.475 kb on + strand at 135.476 kb on - strand at 135.476 kb on - strand at 135.476 kb on - strand at 135.476 kb on - strand at 135.476 kb on - strand at 135.476 kb on - strand at 135.476 kb on - strand at 135.476 kb on - strand at 135.476 kb on - strand at 135.476 kb on - strand at 135.476 kb on - strand at 135.476 kb on - strand at 135.578 kb on - strand at 135.578 kb on - strand at 135.581 kb on + strand at 135.581 kb on + strand at 135.581 kb on + strand at 135.582 kb on - strand at 135.588 kb on + strand at 135.589 kb on - strand at 135.589 kb on - strand at 135.599 kb on + strand at 135.599 kb on + strand at 135.599 kb on + strand at 135.599 kb on + strand at 135.600 kb on - strand at 135.600 kb on - strand at 135.600 kb on - strand at 135.600 kb on - strand at 135.600 kb on - strand at 135.759 kb on + strand at 135.759 kb on + strand at 135.759 kb on + strand at 135.759 kb on + strand at 135.759 kb on + strand at 135.759 kb on + strand at 135.760 kb on - strand at 135.795 kb on + strand at 135.822 kb on + strand at 135.822 kb on + strand at 135.823 kb on - strand at 135.942 kb on + strand, within GFF3310 at 135.942 kb on + strand, within GFF3310 at 135.942 kb on + strand, within GFF3310 at 135.943 kb on - strand, within GFF3310 at 135.943 kb on - strand, within GFF3310 at 135.943 kb on - strand, within GFF3310 at 135.943 kb on - strand, within GFF3310 at 135.963 kb on + strand, within GFF3310 at 135.963 kb on + strand, within GFF3310 at 135.963 kb on + strand, within GFF3310 at 135.964 kb on - strand, within GFF3310 at 135.964 kb on - strand, within GFF3310 at 136.056 kb on + strand, within GFF3310 at 136.056 kb on + strand, within GFF3310 at 136.056 kb on + strand, within GFF3310 at 136.056 kb on + strand, within GFF3310 at 136.056 kb on + strand, within GFF3310 at 136.056 kb on + strand, within GFF3310 at 136.056 kb on + strand, within GFF3310 at 136.057 kb on - strand, within GFF3310 at 136.057 kb on - strand, within GFF3310 at 136.057 kb on - strand, within GFF3310 at 136.057 kb on - strand, within GFF3310 at 136.092 kb on + strand, within GFF3310 at 136.093 kb on - strand, within GFF3310 at 136.093 kb on - strand, within GFF3310 at 136.093 kb on - strand, within GFF3310 at 136.093 kb on - strand, within GFF3310 at 136.099 kb on + strand, within GFF3310 at 136.099 kb on + strand, within GFF3310 at 136.099 kb on + strand, within GFF3310 at 136.099 kb on + strand, within GFF3310 at 136.099 kb on + strand, within GFF3310 at 136.099 kb on + strand, within GFF3310 at 136.099 kb on + strand, within GFF3310 at 136.099 kb on + strand, within GFF3310 at 136.100 kb on - strand, within GFF3310 at 136.100 kb on - strand, within GFF3310 at 136.100 kb on - strand, within GFF3310 at 136.100 kb on - strand, within GFF3310 at 136.100 kb on - strand, within GFF3310 at 136.100 kb on - strand, within GFF3310 at 136.155 kb on + strand, within GFF3310 at 136.155 kb on + strand, within GFF3310 at 136.155 kb on + strand, within GFF3310 at 136.155 kb on + strand, within GFF3310 at 136.155 kb on + strand, within GFF3310 at 136.155 kb on + strand, within GFF3310 at 136.155 kb on + strand, within GFF3310 at 136.155 kb on + strand, within GFF3310 at 136.156 kb on - strand, within GFF3310 at 136.156 kb on - strand, within GFF3310 at 136.156 kb on - strand, within GFF3310 at 136.156 kb on - strand, within GFF3310 at 136.170 kb on + strand, within GFF3310 at 136.170 kb on + strand, within GFF3310 at 136.170 kb on + strand, within GFF3310 at 136.170 kb on + strand, within GFF3310 at 136.170 kb on + strand, within GFF3310 at 136.171 kb on - strand, within GFF3310 at 136.171 kb on - strand, within GFF3310 at 136.171 kb on - strand, within GFF3310 at 136.171 kb on - strand, within GFF3310 at 136.188 kb on + strand, within GFF3310 at 136.189 kb on - strand, within GFF3310 at 136.189 kb on - strand, within GFF3310 at 136.215 kb on + strand, within GFF3310 at 136.215 kb on + strand, within GFF3310 at 136.215 kb on + strand, within GFF3310 at 136.338 kb on + strand, within GFF3310 at 136.338 kb on + strand, within GFF3310 at 136.338 kb on + strand, within GFF3310 at 136.338 kb on + strand, within GFF3310 at 136.338 kb on + strand, within GFF3310 at 136.338 kb on + strand, within GFF3310 at 136.338 kb on + strand, within GFF3310 at 136.339 kb on - strand, within GFF3310 at 136.339 kb on - strand, within GFF3310 at 136.339 kb on - strand, within GFF3310 at 136.342 kb on + strand, within GFF3310 at 136.342 kb on + strand, within GFF3310 at 136.342 kb on + strand, within GFF3310 at 136.342 kb on + strand, within GFF3310 at 136.343 kb on - strand, within GFF3310 at 136.455 kb on + strand, within GFF3310 at 136.456 kb on - strand, within GFF3310 at 136.456 kb on - strand, within GFF3310 at 136.456 kb on - strand, within GFF3310 at 136.456 kb on - strand, within GFF3310 at 136.485 kb on + strand, within GFF3310 at 136.485 kb on + strand, within GFF3310 at 136.485 kb on + strand, within GFF3310 at 136.485 kb on + strand, within GFF3310 at 136.485 kb on + strand, within GFF3310 at 136.485 kb on + strand, within GFF3310 at 136.485 kb on + strand, within GFF3310 at 136.485 kb on + strand, within GFF3310 at 136.485 kb on + strand, within GFF3310 at 136.485 kb on + strand, within GFF3310 at 136.485 kb on + strand, within GFF3310 at 136.485 kb on + strand, within GFF3310 at 136.486 kb on - strand, within GFF3310 at 136.486 kb on - strand, within GFF3310 at 136.486 kb on - strand, within GFF3310 at 136.486 kb on - strand, within GFF3310 at 136.486 kb on - strand, within GFF3310 at 136.486 kb on - strand, within GFF3310 at 136.486 kb on - strand, within GFF3310 at 136.486 kb on - strand, within GFF3310 at 136.486 kb on - strand, within GFF3310 at 136.486 kb on - strand, within GFF3310 at 136.486 kb on - strand, within GFF3310 at 136.509 kb on + strand, within GFF3310 at 136.509 kb on + strand, within GFF3310 at 136.509 kb on + strand, within GFF3310 at 136.509 kb on + strand, within GFF3310 at 136.509 kb on + strand, within GFF3310 at 136.509 kb on + strand, within GFF3310 at 136.509 kb on + strand, within GFF3310 at 136.510 kb on - strand, within GFF3310 at 136.510 kb on - strand, within GFF3310 at 136.510 kb on - strand, within GFF3310 at 136.510 kb on - strand, within GFF3310 at 136.531 kb on - strand, within GFF3310 at 136.531 kb on - strand, within GFF3310 at 136.531 kb on - strand, within GFF3310 at 136.531 kb on - strand, within GFF3310 at 136.531 kb on - strand, within GFF3310 at 136.531 kb on - strand, within GFF3310 at 136.531 kb on - strand, within GFF3310 at 136.531 kb on - strand, within GFF3310 at 136.566 kb on + strand, within GFF3310
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 133,839 - GFF3308 0.69 -0.4 133,865 + GFF3308 0.71 +0.1 133,865 + GFF3308 0.71 +0.2 133,865 + GFF3308 0.71 -1.1 133,865 + GFF3308 0.71 -0.8 133,866 - GFF3308 0.71 -1.8 133,866 - GFF3308 0.71 -0.6 133,866 - GFF3308 0.71 +2.1 133,949 + GFF3308 0.77 +0.2 133,949 + GFF3308 0.77 +0.6 134,031 - GFF3308 0.82 -0.9 134,072 + GFF3308 0.85 +0.5 134,073 - GFF3308 0.85 -0.8 134,138 + GFF3308 0.90 -0.8 134,138 + GFF3308 0.90 -0.4 134,138 + GFF3308 0.90 -0.2 134,138 + GFF3308 0.90 +1.4 134,138 + GFF3308 0.90 +0.6 134,139 - GFF3308 0.90 -0.7 134,139 - GFF3308 0.90 +1.2 134,150 + +0.2 134,151 - -0.2 134,151 - +0.5 134,151 - -1.0 134,151 - -1.1 134,253 - -1.0 134,253 - -0.4 134,253 - +0.8 134,269 + -0.1 134,611 + GFF3309 0.25 -1.6 134,612 - GFF3309 0.25 +0.6 134,899 + GFF3309 0.47 +0.1 134,933 + GFF3309 0.50 -0.1 134,933 + GFF3309 0.50 +1.0 134,933 + GFF3309 0.50 +0.5 134,934 - GFF3309 0.50 -0.3 134,934 - GFF3309 0.50 +2.8 135,060 - GFF3309 0.60 -0.5 135,346 + GFF3309 0.82 +0.3 135,346 + GFF3309 0.82 +0.4 135,347 - GFF3309 0.82 -0.6 135,391 + GFF3309 0.85 -0.6 135,391 + GFF3309 0.85 +1.4 135,392 - GFF3309 0.85 +0.6 135,392 - GFF3309 0.85 -0.1 135,460 + -0.4 135,461 - -1.4 135,461 - +2.6 135,461 - -1.5 135,461 - -0.5 135,475 + +0.9 135,475 + +0.3 135,475 + -0.5 135,476 - -0.4 135,476 - +0.8 135,476 - -0.3 135,476 - +0.3 135,476 - -0.8 135,476 - -0.4 135,476 - +0.0 135,476 - +0.3 135,476 - +0.9 135,476 - +0.5 135,476 - -1.5 135,476 - +0.7 135,578 - -0.4 135,578 - -0.5 135,581 + +0.4 135,581 + +0.6 135,581 + -0.1 135,582 - -0.2 135,588 + +0.8 135,589 - +0.4 135,589 - -1.4 135,599 + -0.2 135,599 + -0.3 135,599 + +0.8 135,599 + -1.2 135,600 - +0.5 135,600 - +0.5 135,600 - -0.3 135,600 - +0.2 135,600 - -0.7 135,759 + +0.6 135,759 + +0.2 135,759 + +0.1 135,759 + -0.6 135,759 + +0.6 135,759 + +0.3 135,760 - -0.6 135,795 + +1.4 135,822 + +0.1 135,822 + -0.5 135,823 - +0.1 135,942 + GFF3310 0.14 -1.2 135,942 + GFF3310 0.14 -0.6 135,942 + GFF3310 0.14 -0.7 135,943 - GFF3310 0.14 +0.3 135,943 - GFF3310 0.14 -0.0 135,943 - GFF3310 0.14 -0.9 135,943 - GFF3310 0.14 -0.2 135,963 + GFF3310 0.15 -0.3 135,963 + GFF3310 0.15 +1.6 135,963 + GFF3310 0.15 +0.3 135,964 - GFF3310 0.15 +0.2 135,964 - GFF3310 0.15 -1.2 136,056 + GFF3310 0.19 +0.1 136,056 + GFF3310 0.19 +0.4 136,056 + GFF3310 0.19 -0.2 136,056 + GFF3310 0.19 -0.5 136,056 + GFF3310 0.19 -0.4 136,056 + GFF3310 0.19 +1.1 136,056 + GFF3310 0.19 -0.2 136,057 - GFF3310 0.19 +0.2 136,057 - GFF3310 0.19 +0.7 136,057 - GFF3310 0.19 -0.7 136,057 - GFF3310 0.19 -0.4 136,092 + GFF3310 0.20 -1.4 136,093 - GFF3310 0.21 -0.5 136,093 - GFF3310 0.21 +0.5 136,093 - GFF3310 0.21 +0.1 136,093 - GFF3310 0.21 -1.5 136,099 + GFF3310 0.21 -0.1 136,099 + GFF3310 0.21 +1.0 136,099 + GFF3310 0.21 -2.1 136,099 + GFF3310 0.21 -0.3 136,099 + GFF3310 0.21 -0.4 136,099 + GFF3310 0.21 -0.6 136,099 + GFF3310 0.21 +1.2 136,099 + GFF3310 0.21 -0.3 136,100 - GFF3310 0.21 -0.2 136,100 - GFF3310 0.21 +0.6 136,100 - GFF3310 0.21 +0.5 136,100 - GFF3310 0.21 -0.8 136,100 - GFF3310 0.21 +1.5 136,100 - GFF3310 0.21 -0.4 136,155 + GFF3310 0.23 -0.2 136,155 + GFF3310 0.23 -0.1 136,155 + GFF3310 0.23 +0.0 136,155 + GFF3310 0.23 -0.4 136,155 + GFF3310 0.23 -0.1 136,155 + GFF3310 0.23 -1.6 136,155 + GFF3310 0.23 -0.4 136,155 + GFF3310 0.23 -1.2 136,156 - GFF3310 0.23 -0.0 136,156 - GFF3310 0.23 -0.1 136,156 - GFF3310 0.23 -0.3 136,156 - GFF3310 0.23 -0.4 136,170 + GFF3310 0.24 +0.2 136,170 + GFF3310 0.24 -0.1 136,170 + GFF3310 0.24 -1.0 136,170 + GFF3310 0.24 -0.5 136,170 + GFF3310 0.24 -0.3 136,171 - GFF3310 0.24 -0.6 136,171 - GFF3310 0.24 -0.4 136,171 - GFF3310 0.24 +0.1 136,171 - GFF3310 0.24 +1.8 136,188 + GFF3310 0.24 +0.2 136,189 - GFF3310 0.25 +0.1 136,189 - GFF3310 0.25 -1.2 136,215 + GFF3310 0.26 -0.4 136,215 + GFF3310 0.26 +0.9 136,215 + GFF3310 0.26 -1.0 136,338 + GFF3310 0.31 -0.9 136,338 + GFF3310 0.31 -0.6 136,338 + GFF3310 0.31 -0.5 136,338 + GFF3310 0.31 +0.8 136,338 + GFF3310 0.31 -0.8 136,338 + GFF3310 0.31 +0.3 136,338 + GFF3310 0.31 -2.3 136,339 - GFF3310 0.31 +0.5 136,339 - GFF3310 0.31 -0.6 136,339 - GFF3310 0.31 -0.4 136,342 + GFF3310 0.31 +0.2 136,342 + GFF3310 0.31 -0.1 136,342 + GFF3310 0.31 +0.6 136,342 + GFF3310 0.31 +0.6 136,343 - GFF3310 0.31 -0.3 136,455 + GFF3310 0.36 -1.6 136,456 - GFF3310 0.36 -0.5 136,456 - GFF3310 0.36 -0.5 136,456 - GFF3310 0.36 +0.7 136,456 - GFF3310 0.36 -0.1 136,485 + GFF3310 0.37 +0.0 136,485 + GFF3310 0.37 -0.2 136,485 + GFF3310 0.37 -0.3 136,485 + GFF3310 0.37 -1.4 136,485 + GFF3310 0.37 -0.9 136,485 + GFF3310 0.37 -0.2 136,485 + GFF3310 0.37 -1.0 136,485 + GFF3310 0.37 +0.1 136,485 + GFF3310 0.37 +0.3 136,485 + GFF3310 0.37 -0.2 136,485 + GFF3310 0.37 +0.2 136,485 + GFF3310 0.37 +1.2 136,486 - GFF3310 0.37 -0.4 136,486 - GFF3310 0.37 -0.3 136,486 - GFF3310 0.37 +0.3 136,486 - GFF3310 0.37 -0.7 136,486 - GFF3310 0.37 +0.7 136,486 - GFF3310 0.37 -0.4 136,486 - GFF3310 0.37 +0.3 136,486 - GFF3310 0.37 -0.2 136,486 - GFF3310 0.37 -0.1 136,486 - GFF3310 0.37 +0.2 136,486 - GFF3310 0.37 -0.1 136,509 + GFF3310 0.38 -1.3 136,509 + GFF3310 0.38 +1.1 136,509 + GFF3310 0.38 -0.7 136,509 + GFF3310 0.38 -0.0 136,509 + GFF3310 0.38 -0.4 136,509 + GFF3310 0.38 +0.1 136,509 + GFF3310 0.38 +1.8 136,510 - GFF3310 0.38 -0.2 136,510 - GFF3310 0.38 +0.3 136,510 - GFF3310 0.38 +0.5 136,510 - GFF3310 0.38 -0.2 136,531 - GFF3310 0.39 +1.4 136,531 - GFF3310 0.39 +0.2 136,531 - GFF3310 0.39 -0.2 136,531 - GFF3310 0.39 -0.2 136,531 - GFF3310 0.39 +0.8 136,531 - GFF3310 0.39 +0.5 136,531 - GFF3310 0.39 +1.4 136,531 - GFF3310 0.39 +0.1 136,566 + GFF3310 0.40 -0.6
Or see this region's nucleotide sequence