Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt GFF3273 and GFF3274 are separated by 103 nucleotides GFF3274 and GFF3275 are separated by 89 nucleotides 
        GFF3273 - Translation initiation factor 2, at 93,281 to 94,216 
        GFF3273 
         
        
        GFF3274 - cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases, at 94,320 to 94,781 
        GFF3274 
         
        
        GFF3275 - tRNA-5-carboxymethylaminomethyl-2-thiouridine(34) synthesis protein MnmE, at 94,871 to 96,277 
        GFF3275 
         Position (kb) 94 
95 Strain fitness (log2 ratio) -3 
-2 
-1 
0 
1 
2 
3 
4 at 93.363 kb on + strand at 93.363 kb on + strand at 93.363 kb on + strand at 93.363 kb on + strand at 93.363 kb on + strand at 93.363 kb on + strand at 93.363 kb on + strand at 93.363 kb on + strand at 93.363 kb on + strand at 93.363 kb on + strand at 93.363 kb on + strand at 93.363 kb on + strand at 93.364 kb on - strand at 93.364 kb on - strand at 93.364 kb on - strand at 93.364 kb on - strand at 93.364 kb on - strand at 93.364 kb on - strand at 93.364 kb on - strand at 93.364 kb on - strand at 93.364 kb on - strand at 93.364 kb on - strand at 93.364 kb on - strand at 93.364 kb on - strand at 93.364 kb on - strand at 93.571 kb on + strand, within GFF3273 at 93.750 kb on + strand, within GFF3273 at 93.750 kb on + strand, within GFF3273 at 93.750 kb on + strand, within GFF3273 at 93.751 kb on - strand, within GFF3273 at 93.751 kb on - strand, within GFF3273 at 93.751 kb on - strand, within GFF3273 at 93.942 kb on + strand, within GFF3273 at 93.942 kb on + strand, within GFF3273 at 93.943 kb on - strand, within GFF3273 at 93.943 kb on - strand, within GFF3273 at 93.943 kb on - strand, within GFF3273 at 93.943 kb on - strand, within GFF3273 at 94.221 kb on + strand at 94.221 kb on + strand at 94.221 kb on + strand at 94.221 kb on + strand at 94.221 kb on + strand at 94.221 kb on + strand at 94.221 kb on + strand at 94.221 kb on + strand at 94.222 kb on - strand at 94.222 kb on - strand at 94.222 kb on - strand at 94.222 kb on - strand at 94.222 kb on - strand at 94.224 kb on + strand at 94.224 kb on + strand at 94.224 kb on + strand at 94.224 kb on + strand at 94.224 kb on + strand at 94.224 kb on + strand at 94.224 kb on + strand at 94.224 kb on + strand at 94.224 kb on + strand at 94.224 kb on + strand at 94.225 kb on - strand at 94.225 kb on - strand at 94.225 kb on - strand at 94.225 kb on - strand at 94.231 kb on + strand at 94.231 kb on + strand at 94.231 kb on + strand at 94.231 kb on + strand at 94.231 kb on + strand at 94.231 kb on + strand at 94.231 kb on + strand at 94.231 kb on + strand at 94.231 kb on + strand at 94.232 kb on - strand at 94.232 kb on - strand at 94.232 kb on - strand at 94.232 kb on - strand at 94.232 kb on - strand at 94.232 kb on - strand at 94.232 kb on - strand at 94.232 kb on - strand at 94.238 kb on + strand at 94.238 kb on + strand at 94.238 kb on + strand at 94.238 kb on + strand at 94.238 kb on + strand at 94.239 kb on - strand at 94.239 kb on - strand at 94.239 kb on - strand at 94.239 kb on - strand at 94.239 kb on - strand at 94.239 kb on - strand at 94.239 kb on - strand at 94.239 kb on - strand at 94.239 kb on - strand at 94.239 kb on - strand at 94.239 kb on - strand at 94.668 kb on + strand, within GFF3274 at 94.668 kb on + strand, within GFF3274 at 94.668 kb on + strand, within GFF3274 at 94.669 kb on - strand, within GFF3274 at 94.669 kb on - strand, within GFF3274 at 94.669 kb on - strand, within GFF3274 at 94.669 kb on - strand, within GFF3274 at 94.669 kb on - strand, within GFF3274 at 94.669 kb on - strand, within GFF3274 at 94.682 kb on - strand, within GFF3274 at 94.682 kb on - strand, within GFF3274 at 94.773 kb on + strand at 94.773 kb on + strand at 94.773 kb on + strand at 94.773 kb on + strand at 94.774 kb on - strand at 94.774 kb on - strand at 94.774 kb on - strand at 94.774 kb on - strand at 94.813 kb on + strand at 94.813 kb on + strand at 94.814 kb on - strand at 94.814 kb on - strand at 94.814 kb on - strand at 94.840 kb on - strand at 94.840 kb on - strand at 94.840 kb on - strand at 94.840 kb on - strand at 94.840 kb on - strand at 94.840 kb on - strand at 94.840 kb on - strand at 94.872 kb on + strand at 94.872 kb on + strand at 94.873 kb on - strand at 94.873 kb on - strand at 94.873 kb on - strand at 94.873 kb on - strand at 94.873 kb on - strand at 94.873 kb on - strand at 95.082 kb on + strand, within GFF3275 at 95.083 kb on - strand, within GFF3275  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 93,363 +  +0.8 93,363 +  -0.4 93,363 +  -1.2 93,363 +  +0.0 93,363 +  -0.2 93,363 +  +1.0 93,363 +  -0.8 93,363 +  -1.9 93,363 +  +1.8 93,363 +  -0.4 93,363 +  +1.1 93,363 +  -0.1 93,364 -  -0.2 93,364 -  -0.3 93,364 -  -0.3 93,364 -  -0.1 93,364 -  -1.3 93,364 -  +0.6 93,364 -  +0.7 93,364 -  -1.4 93,364 -  -0.3 93,364 -  +0.9 93,364 -  -2.2 93,364 -  -0.3 93,364 -  -1.2 93,571 +  GFF3273 0.31  -0.3 93,750 +  GFF3273 0.50  +0.5 93,750 +  GFF3273 0.50  -0.4 93,750 +  GFF3273 0.50  -0.8 93,751 -  GFF3273 0.50  -0.4 93,751 -  GFF3273 0.50  -0.4 93,751 -  GFF3273 0.50  +1.7 93,942 +  GFF3273 0.71  -2.1 93,942 +  GFF3273 0.71  +0.4 93,943 -  GFF3273 0.71  -0.1 93,943 -  GFF3273 0.71  +0.5 93,943 -  GFF3273 0.71  -0.5 93,943 -  GFF3273 0.71  -0.5 94,221 +  +0.8 94,221 +  +0.1 94,221 +  -0.0 94,221 +  +0.1 94,221 +  -0.5 94,221 +  +3.8 94,221 +  +0.2 94,221 +  +0.7 94,222 -  +0.9 94,222 -  -0.3 94,222 -  -1.0 94,222 -  -0.0 94,222 -  -0.6 94,224 +  -0.4 94,224 +  -1.5 94,224 +  -0.3 94,224 +  -1.4 94,224 +  -0.7 94,224 +  +0.0 94,224 +  +0.1 94,224 +  -1.8 94,224 +  -0.7 94,224 +  -0.8 94,225 -  -0.1 94,225 -  -0.1 94,225 -  -0.3 94,225 -  -0.2 94,231 +  +0.0 94,231 +  +0.0 94,231 +  -0.4 94,231 +  +0.2 94,231 +  -0.5 94,231 +  -0.6 94,231 +  -0.1 94,231 +  +0.4 94,231 +  +0.2 94,232 -  -0.5 94,232 -  -0.5 94,232 -  -2.4 94,232 -  +0.6 94,232 -  +0.0 94,232 -  +0.3 94,232 -  -0.9 94,232 -  -1.5 94,238 +  -0.1 94,238 +  -0.2 94,238 +  -1.5 94,238 +  -0.7 94,238 +  +0.0 94,239 -  -1.3 94,239 -  -0.4 94,239 -  -0.2 94,239 -  -0.2 94,239 -  -0.3 94,239 -  +0.8 94,239 -  -1.4 94,239 -  +0.1 94,239 -  -0.7 94,239 -  -0.5 94,239 -  -1.0 94,668 +  GFF3274 0.75  -0.5 94,668 +  GFF3274 0.75  -2.8 94,668 +  GFF3274 0.75  -0.1 94,669 -  GFF3274 0.76  -1.1 94,669 -  GFF3274 0.76  +0.1 94,669 -  GFF3274 0.76  -1.4 94,669 -  GFF3274 0.76  -0.2 94,669 -  GFF3274 0.76  -0.5 94,669 -  GFF3274 0.76  +0.0 94,682 -  GFF3274 0.78  -0.5 94,682 -  GFF3274 0.78  -1.4 94,773 +  -0.3 94,773 +  -1.2 94,773 +  +0.2 94,773 +  +0.1 94,774 -  +0.5 94,774 -  -0.4 94,774 -  +0.3 94,774 -  -0.1 94,813 +  -1.2 94,813 +  -0.5 94,814 -  -1.4 94,814 -  -0.9 94,814 -  -0.7 94,840 -  +0.2 94,840 -  -0.0 94,840 -  +0.0 94,840 -  -0.3 94,840 -  -0.2 94,840 -  -0.3 94,840 -  -0.3 94,872 +  -1.3 94,872 +  -1.0 94,873 -  -1.2 94,873 -  -0.1 94,873 -  -0.3 94,873 -  +1.3 94,873 -  -0.4 94,873 -  -0.3 95,082 +  GFF3275 0.15  -1.2 95,083 -  GFF3275 0.15  -0.5 
Or see this region's nucleotide sequence