Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF319 and GFF320 overlap by 4 nucleotides GFF320 and GFF321 overlap by 19 nucleotides GFF321 and GFF322 are separated by 33 nucleotides GFF322 and GFF323 overlap by 4 nucleotides
GFF319 - EXTRACYTOPLASMIC FUNCTION ALTERNATIVE SIGMA FACTOR, at 352,499 to 353,140
GFF319
GFF320 - RNA polymerase ECF-type sigma factor, at 353,137 to 353,727
GFF320
GFF321 - hypothetical protein, at 353,709 to 353,975
GFF321
GFF322 - GTP cyclohydrolase II homolog, at 354,009 to 355,151
GFF322
GFF323 - Possible pyrimidine-degrading protein DUF1688, at 355,148 to 356,554
GFF323
Position (kb) 354
355
356 Strain fitness (log2 ratio) -2
-1
0
1 at 353.283 kb on + strand, within GFF320 at 353.283 kb on + strand, within GFF320 at 353.283 kb on + strand, within GFF320 at 353.284 kb on - strand, within GFF320 at 353.284 kb on - strand, within GFF320 at 353.447 kb on + strand, within GFF320 at 353.447 kb on + strand, within GFF320 at 353.447 kb on + strand, within GFF320 at 353.448 kb on - strand, within GFF320 at 353.448 kb on - strand, within GFF320 at 353.495 kb on + strand, within GFF320 at 353.495 kb on + strand, within GFF320 at 353.495 kb on + strand, within GFF320 at 353.495 kb on + strand, within GFF320 at 353.496 kb on - strand, within GFF320 at 353.496 kb on - strand, within GFF320 at 353.496 kb on - strand, within GFF320 at 353.710 kb on + strand at 353.710 kb on + strand at 353.710 kb on + strand at 353.711 kb on - strand at 353.711 kb on - strand at 353.711 kb on - strand at 353.815 kb on + strand, within GFF321 at 353.815 kb on + strand, within GFF321 at 353.815 kb on + strand, within GFF321 at 353.815 kb on + strand, within GFF321 at 353.815 kb on + strand, within GFF321 at 353.815 kb on + strand, within GFF321 at 353.815 kb on + strand, within GFF321 at 353.815 kb on + strand, within GFF321 at 353.816 kb on - strand, within GFF321 at 353.816 kb on - strand, within GFF321 at 353.816 kb on - strand, within GFF321 at 353.816 kb on - strand, within GFF321 at 353.816 kb on - strand, within GFF321 at 353.816 kb on - strand, within GFF321 at 354.049 kb on + strand at 354.049 kb on + strand at 354.049 kb on + strand at 354.049 kb on + strand at 354.049 kb on + strand at 354.049 kb on + strand at 354.049 kb on + strand at 354.050 kb on - strand at 354.050 kb on - strand at 354.050 kb on - strand at 354.050 kb on - strand at 354.050 kb on - strand at 354.050 kb on - strand at 354.050 kb on - strand at 354.069 kb on + strand at 354.069 kb on + strand at 354.069 kb on + strand at 354.069 kb on + strand at 354.070 kb on - strand at 354.070 kb on - strand at 354.070 kb on - strand at 354.070 kb on - strand at 354.070 kb on - strand at 354.070 kb on - strand at 354.070 kb on - strand at 354.079 kb on - strand at 354.079 kb on - strand at 354.261 kb on + strand, within GFF322 at 354.261 kb on + strand, within GFF322 at 354.417 kb on + strand, within GFF322 at 354.418 kb on - strand, within GFF322 at 354.498 kb on + strand, within GFF322 at 354.498 kb on + strand, within GFF322 at 354.498 kb on + strand, within GFF322 at 354.499 kb on - strand, within GFF322 at 354.499 kb on - strand, within GFF322 at 354.499 kb on - strand, within GFF322 at 354.499 kb on - strand, within GFF322 at 354.564 kb on + strand, within GFF322 at 354.564 kb on + strand, within GFF322 at 354.564 kb on + strand, within GFF322 at 354.564 kb on + strand, within GFF322 at 354.564 kb on + strand, within GFF322 at 354.564 kb on + strand, within GFF322 at 354.565 kb on - strand, within GFF322 at 354.565 kb on - strand, within GFF322 at 354.565 kb on - strand, within GFF322 at 354.565 kb on - strand, within GFF322 at 354.565 kb on - strand, within GFF322 at 354.681 kb on + strand, within GFF322 at 354.681 kb on + strand, within GFF322 at 354.682 kb on - strand, within GFF322 at 354.798 kb on + strand, within GFF322 at 354.798 kb on + strand, within GFF322 at 354.798 kb on + strand, within GFF322 at 354.799 kb on - strand, within GFF322 at 354.799 kb on - strand, within GFF322 at 354.799 kb on - strand, within GFF322 at 354.843 kb on + strand, within GFF322 at 354.844 kb on - strand, within GFF322 at 355.080 kb on + strand at 355.081 kb on - strand at 355.081 kb on - strand at 355.242 kb on - strand at 355.242 kb on - strand at 355.613 kb on + strand, within GFF323 at 355.613 kb on + strand, within GFF323 at 355.613 kb on + strand, within GFF323 at 355.614 kb on - strand, within GFF323 at 355.674 kb on - strand, within GFF323 at 355.674 kb on - strand, within GFF323 at 355.674 kb on - strand, within GFF323 at 355.674 kb on - strand, within GFF323 at 355.674 kb on - strand, within GFF323 at 355.722 kb on - strand, within GFF323 at 355.722 kb on - strand, within GFF323
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 353,283 + GFF320 0.25 -0.6 353,283 + GFF320 0.25 -0.9 353,283 + GFF320 0.25 +0.4 353,284 - GFF320 0.25 -0.1 353,284 - GFF320 0.25 -0.3 353,447 + GFF320 0.52 -1.2 353,447 + GFF320 0.52 -0.3 353,447 + GFF320 0.52 -0.3 353,448 - GFF320 0.53 +0.5 353,448 - GFF320 0.53 -0.7 353,495 + GFF320 0.61 +0.3 353,495 + GFF320 0.61 +0.8 353,495 + GFF320 0.61 +0.6 353,495 + GFF320 0.61 +1.6 353,496 - GFF320 0.61 +0.5 353,496 - GFF320 0.61 +1.2 353,496 - GFF320 0.61 -0.5 353,710 + +0.2 353,710 + +0.2 353,710 + -0.3 353,711 - +0.1 353,711 - +0.3 353,711 - -0.5 353,815 + GFF321 0.40 -0.5 353,815 + GFF321 0.40 +0.3 353,815 + GFF321 0.40 -0.6 353,815 + GFF321 0.40 -0.5 353,815 + GFF321 0.40 -0.4 353,815 + GFF321 0.40 -0.4 353,815 + GFF321 0.40 -0.3 353,815 + GFF321 0.40 -0.7 353,816 - GFF321 0.40 -1.1 353,816 - GFF321 0.40 -0.7 353,816 - GFF321 0.40 +0.3 353,816 - GFF321 0.40 +0.5 353,816 - GFF321 0.40 -0.8 353,816 - GFF321 0.40 -1.4 354,049 + -0.2 354,049 + +0.4 354,049 + -1.3 354,049 + +0.6 354,049 + -0.8 354,049 + -1.1 354,049 + -1.0 354,050 - -0.5 354,050 - +0.5 354,050 - -0.3 354,050 - +0.4 354,050 - -0.7 354,050 - -0.6 354,050 - -0.2 354,069 + +0.3 354,069 + +0.2 354,069 + -0.7 354,069 + -0.8 354,070 - +0.4 354,070 - -0.3 354,070 - -0.0 354,070 - -0.8 354,070 - -0.2 354,070 - +0.4 354,070 - +0.5 354,079 - -1.0 354,079 - +0.2 354,261 + GFF322 0.22 +0.1 354,261 + GFF322 0.22 -0.4 354,417 + GFF322 0.36 -0.7 354,418 - GFF322 0.36 +0.6 354,498 + GFF322 0.43 -0.0 354,498 + GFF322 0.43 +1.0 354,498 + GFF322 0.43 -0.4 354,499 - GFF322 0.43 -0.5 354,499 - GFF322 0.43 +1.0 354,499 - GFF322 0.43 -1.3 354,499 - GFF322 0.43 -0.0 354,564 + GFF322 0.49 -2.0 354,564 + GFF322 0.49 -1.1 354,564 + GFF322 0.49 -0.8 354,564 + GFF322 0.49 +0.2 354,564 + GFF322 0.49 +0.1 354,564 + GFF322 0.49 -0.3 354,565 - GFF322 0.49 -0.5 354,565 - GFF322 0.49 -0.5 354,565 - GFF322 0.49 +0.3 354,565 - GFF322 0.49 -0.7 354,565 - GFF322 0.49 -0.0 354,681 + GFF322 0.59 -1.1 354,681 + GFF322 0.59 -0.4 354,682 - GFF322 0.59 +0.5 354,798 + GFF322 0.69 -1.5 354,798 + GFF322 0.69 -0.8 354,798 + GFF322 0.69 -1.4 354,799 - GFF322 0.69 -0.5 354,799 - GFF322 0.69 -0.4 354,799 - GFF322 0.69 -0.1 354,843 + GFF322 0.73 -1.6 354,844 - GFF322 0.73 +0.0 355,080 + +0.2 355,081 - -0.4 355,081 - -0.2 355,242 - +0.8 355,242 - -0.2 355,613 + GFF323 0.33 +1.2 355,613 + GFF323 0.33 -0.6 355,613 + GFF323 0.33 +0.8 355,614 - GFF323 0.33 -0.9 355,674 - GFF323 0.37 -0.1 355,674 - GFF323 0.37 -0.5 355,674 - GFF323 0.37 +0.2 355,674 - GFF323 0.37 -0.8 355,674 - GFF323 0.37 -1.0 355,722 - GFF323 0.41 +0.1 355,722 - GFF323 0.41 -0.1
Or see this region's nucleotide sequence