Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3212 and GFF3213 are separated by 10 nucleotides GFF3213 and GFF3214 overlap by 1 nucleotides GFF3214 and GFF3215 overlap by 4 nucleotides GFF3215 and GFF3216 are separated by 188 nucleotides GFF3216 and GFF3217 are separated by 6 nucleotides
GFF3212 - Oxidoreductase, at 32,977 to 34,044
GFF3212
GFF3213 - no description, at 34,055 to 34,423
GFF3213
GFF3214 - FIG138928: iron-regulated membrane protein, at 34,423 to 36,090
GFF3214
GFF3215 - no description, at 36,087 to 36,398
GFF3215
GFF3216 - hypothetical protein, at 36,587 to 37,069
GFF3216
GFF3217 - hypothetical protein, at 37,076 to 37,687
GFF3217
Position (kb) 34
35
36
37 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 33.433 kb on + strand, within GFF3212 at 33.434 kb on - strand, within GFF3212 at 33.643 kb on + strand, within GFF3212 at 33.643 kb on + strand, within GFF3212 at 33.644 kb on - strand, within GFF3212 at 33.644 kb on - strand, within GFF3212 at 33.644 kb on - strand, within GFF3212 at 34.086 kb on + strand at 34.087 kb on - strand at 34.087 kb on - strand at 34.087 kb on - strand at 34.904 kb on - strand, within GFF3214 at 34.904 kb on - strand, within GFF3214 at 35.141 kb on + strand, within GFF3214 at 35.141 kb on + strand, within GFF3214 at 35.372 kb on + strand, within GFF3214 at 35.372 kb on + strand, within GFF3214 at 35.372 kb on + strand, within GFF3214 at 35.373 kb on - strand, within GFF3214 at 35.373 kb on - strand, within GFF3214 at 35.399 kb on + strand, within GFF3214 at 35.399 kb on + strand, within GFF3214 at 35.399 kb on + strand, within GFF3214 at 35.399 kb on + strand, within GFF3214 at 35.399 kb on + strand, within GFF3214 at 35.399 kb on + strand, within GFF3214 at 35.399 kb on + strand, within GFF3214 at 35.399 kb on + strand, within GFF3214 at 35.399 kb on + strand, within GFF3214 at 35.400 kb on - strand, within GFF3214 at 35.400 kb on - strand, within GFF3214 at 35.400 kb on - strand, within GFF3214 at 35.400 kb on - strand, within GFF3214 at 35.400 kb on - strand, within GFF3214 at 35.400 kb on - strand, within GFF3214 at 35.400 kb on - strand, within GFF3214 at 35.429 kb on + strand, within GFF3214 at 35.429 kb on + strand, within GFF3214 at 35.429 kb on + strand, within GFF3214 at 35.429 kb on + strand, within GFF3214 at 35.429 kb on + strand, within GFF3214 at 35.430 kb on - strand, within GFF3214 at 35.430 kb on - strand, within GFF3214 at 35.447 kb on + strand, within GFF3214 at 35.448 kb on - strand, within GFF3214 at 35.448 kb on - strand, within GFF3214 at 35.630 kb on + strand, within GFF3214 at 35.630 kb on + strand, within GFF3214 at 35.630 kb on + strand, within GFF3214 at 35.630 kb on + strand, within GFF3214 at 35.630 kb on + strand, within GFF3214 at 35.631 kb on - strand, within GFF3214 at 35.631 kb on - strand, within GFF3214 at 35.631 kb on - strand, within GFF3214 at 35.631 kb on - strand, within GFF3214 at 35.631 kb on - strand, within GFF3214 at 35.631 kb on - strand, within GFF3214 at 35.631 kb on - strand, within GFF3214 at 35.631 kb on - strand, within GFF3214 at 35.631 kb on - strand, within GFF3214 at 35.631 kb on - strand, within GFF3214 at 35.631 kb on - strand, within GFF3214 at 35.631 kb on - strand, within GFF3214 at 35.631 kb on - strand, within GFF3214 at 35.655 kb on - strand, within GFF3214 at 35.889 kb on - strand, within GFF3214 at 35.889 kb on - strand, within GFF3214 at 35.987 kb on + strand at 35.987 kb on + strand at 35.987 kb on + strand at 35.988 kb on - strand at 35.988 kb on - strand at 36.379 kb on + strand at 36.565 kb on + strand at 36.639 kb on + strand, within GFF3216 at 36.639 kb on + strand, within GFF3216 at 36.640 kb on - strand, within GFF3216 at 36.640 kb on - strand, within GFF3216 at 36.640 kb on - strand, within GFF3216 at 36.640 kb on - strand, within GFF3216 at 36.702 kb on + strand, within GFF3216 at 36.702 kb on + strand, within GFF3216 at 36.702 kb on + strand, within GFF3216 at 36.703 kb on - strand, within GFF3216 at 36.703 kb on - strand, within GFF3216 at 36.703 kb on - strand, within GFF3216 at 36.846 kb on - strand, within GFF3216 at 37.077 kb on + strand at 37.077 kb on + strand at 37.078 kb on - strand at 37.078 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 33,433 + GFF3212 0.43 +0.8 33,434 - GFF3212 0.43 -1.2 33,643 + GFF3212 0.62 +0.4 33,643 + GFF3212 0.62 -0.0 33,644 - GFF3212 0.62 +0.6 33,644 - GFF3212 0.62 -2.8 33,644 - GFF3212 0.62 -0.0 34,086 + -0.1 34,087 - -0.9 34,087 - -0.5 34,087 - -0.1 34,904 - GFF3214 0.29 +0.0 34,904 - GFF3214 0.29 -0.5 35,141 + GFF3214 0.43 +0.8 35,141 + GFF3214 0.43 +1.2 35,372 + GFF3214 0.57 -0.6 35,372 + GFF3214 0.57 +0.4 35,372 + GFF3214 0.57 -0.1 35,373 - GFF3214 0.57 +0.7 35,373 - GFF3214 0.57 +1.5 35,399 + GFF3214 0.59 +1.8 35,399 + GFF3214 0.59 -0.3 35,399 + GFF3214 0.59 +0.0 35,399 + GFF3214 0.59 +0.1 35,399 + GFF3214 0.59 -0.8 35,399 + GFF3214 0.59 +0.3 35,399 + GFF3214 0.59 -1.0 35,399 + GFF3214 0.59 -0.2 35,399 + GFF3214 0.59 +1.0 35,400 - GFF3214 0.59 -1.4 35,400 - GFF3214 0.59 +0.2 35,400 - GFF3214 0.59 +0.4 35,400 - GFF3214 0.59 -2.2 35,400 - GFF3214 0.59 +1.6 35,400 - GFF3214 0.59 -0.3 35,400 - GFF3214 0.59 +0.6 35,429 + GFF3214 0.60 +0.8 35,429 + GFF3214 0.60 -0.2 35,429 + GFF3214 0.60 +0.4 35,429 + GFF3214 0.60 -0.0 35,429 + GFF3214 0.60 -0.5 35,430 - GFF3214 0.60 +0.8 35,430 - GFF3214 0.60 -0.7 35,447 + GFF3214 0.61 -0.2 35,448 - GFF3214 0.61 -0.9 35,448 - GFF3214 0.61 -0.4 35,630 + GFF3214 0.72 -0.5 35,630 + GFF3214 0.72 -0.5 35,630 + GFF3214 0.72 -0.6 35,630 + GFF3214 0.72 -0.5 35,630 + GFF3214 0.72 -2.1 35,631 - GFF3214 0.72 -0.1 35,631 - GFF3214 0.72 -1.0 35,631 - GFF3214 0.72 +0.4 35,631 - GFF3214 0.72 +0.3 35,631 - GFF3214 0.72 -0.8 35,631 - GFF3214 0.72 -0.4 35,631 - GFF3214 0.72 -0.2 35,631 - GFF3214 0.72 -0.5 35,631 - GFF3214 0.72 -0.5 35,631 - GFF3214 0.72 -1.0 35,631 - GFF3214 0.72 -2.6 35,631 - GFF3214 0.72 -0.6 35,631 - GFF3214 0.72 -0.8 35,655 - GFF3214 0.74 -0.7 35,889 - GFF3214 0.88 -0.5 35,889 - GFF3214 0.88 -0.1 35,987 + +0.6 35,987 + -0.7 35,987 + -0.9 35,988 - -0.4 35,988 - -1.5 36,379 + +0.2 36,565 + +0.1 36,639 + GFF3216 0.11 -0.4 36,639 + GFF3216 0.11 -0.2 36,640 - GFF3216 0.11 +0.6 36,640 - GFF3216 0.11 -0.3 36,640 - GFF3216 0.11 +2.8 36,640 - GFF3216 0.11 -1.5 36,702 + GFF3216 0.24 +0.1 36,702 + GFF3216 0.24 -0.3 36,702 + GFF3216 0.24 -0.5 36,703 - GFF3216 0.24 -1.8 36,703 - GFF3216 0.24 -0.7 36,703 - GFF3216 0.24 -0.2 36,846 - GFF3216 0.54 -0.9 37,077 + +0.0 37,077 + +0.1 37,078 - -0.5 37,078 - +0.1
Or see this region's nucleotide sequence