Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3175 and GFF3176 are separated by 18 nucleotides GFF3176 and GFF3177 are separated by 162 nucleotides GFF3177 and GFF3178 are separated by 289 nucleotides
GFF3175 - Uncharacterized MFS-type transporter, at 615,966 to 617,279
GFF3175
GFF3176 - Two-component transcriptional response regulator, LuxR family, at 617,298 to 617,771
GFF3176
GFF3177 - hypothetical protein, at 617,934 to 618,158
GFF3177
GFF3178 - hypothetical protein, at 618,448 to 621,555
GFF3178
Position (kb) 617
618
619 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 617.001 kb on + strand, within GFF3175 at 617.001 kb on + strand, within GFF3175 at 617.002 kb on - strand, within GFF3175 at 617.002 kb on - strand, within GFF3175 at 617.086 kb on - strand, within GFF3175 at 617.181 kb on + strand at 617.181 kb on + strand at 617.181 kb on + strand at 617.181 kb on + strand at 617.181 kb on + strand at 617.181 kb on + strand at 617.181 kb on + strand at 617.181 kb on + strand at 617.181 kb on + strand at 617.182 kb on - strand at 617.182 kb on - strand at 617.182 kb on - strand at 617.182 kb on - strand at 617.182 kb on - strand at 617.285 kb on + strand at 617.285 kb on + strand at 617.368 kb on + strand, within GFF3176 at 617.368 kb on + strand, within GFF3176 at 617.368 kb on + strand, within GFF3176 at 617.368 kb on + strand, within GFF3176 at 617.368 kb on + strand, within GFF3176 at 617.368 kb on + strand, within GFF3176 at 617.369 kb on - strand, within GFF3176 at 617.369 kb on - strand, within GFF3176 at 617.369 kb on - strand, within GFF3176 at 617.369 kb on - strand, within GFF3176 at 617.369 kb on - strand, within GFF3176 at 617.369 kb on - strand, within GFF3176 at 617.406 kb on + strand, within GFF3176 at 617.406 kb on + strand, within GFF3176 at 617.587 kb on + strand, within GFF3176 at 617.588 kb on - strand, within GFF3176 at 617.588 kb on - strand, within GFF3176 at 617.794 kb on + strand at 617.809 kb on - strand at 617.869 kb on + strand at 617.870 kb on - strand at 617.870 kb on - strand at 617.870 kb on - strand at 617.870 kb on - strand at 617.946 kb on + strand at 617.946 kb on + strand at 617.946 kb on + strand at 617.947 kb on - strand at 618.113 kb on + strand, within GFF3177 at 618.178 kb on + strand at 618.179 kb on - strand at 618.207 kb on + strand at 618.208 kb on - strand at 618.208 kb on - strand at 618.208 kb on - strand at 618.261 kb on + strand at 618.262 kb on - strand at 618.285 kb on + strand at 618.300 kb on + strand at 618.316 kb on + strand at 618.329 kb on + strand at 618.335 kb on + strand at 618.335 kb on + strand at 618.335 kb on + strand at 618.405 kb on + strand at 618.991 kb on + strand, within GFF3178 at 618.991 kb on + strand, within GFF3178 at 618.992 kb on - strand, within GFF3178 at 618.992 kb on - strand, within GFF3178 at 619.093 kb on + strand, within GFF3178 at 619.093 kb on + strand, within GFF3178 at 619.094 kb on - strand, within GFF3178 at 619.094 kb on - strand, within GFF3178 at 619.094 kb on - strand, within GFF3178
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 617,001 + GFF3175 0.79 +0.2 617,001 + GFF3175 0.79 +0.2 617,002 - GFF3175 0.79 -1.8 617,002 - GFF3175 0.79 -0.1 617,086 - GFF3175 0.85 -0.6 617,181 + -0.4 617,181 + +1.0 617,181 + +0.0 617,181 + -0.3 617,181 + -1.4 617,181 + -0.5 617,181 + -0.4 617,181 + -0.5 617,181 + +0.7 617,182 - -1.3 617,182 - +1.6 617,182 - -0.0 617,182 - +0.3 617,182 - -1.8 617,285 + +0.0 617,285 + -1.4 617,368 + GFF3176 0.15 -0.6 617,368 + GFF3176 0.15 -0.7 617,368 + GFF3176 0.15 -1.3 617,368 + GFF3176 0.15 +0.4 617,368 + GFF3176 0.15 -0.8 617,368 + GFF3176 0.15 -0.2 617,369 - GFF3176 0.15 +2.1 617,369 - GFF3176 0.15 -0.7 617,369 - GFF3176 0.15 -0.2 617,369 - GFF3176 0.15 -0.3 617,369 - GFF3176 0.15 -1.4 617,369 - GFF3176 0.15 +0.1 617,406 + GFF3176 0.23 -0.6 617,406 + GFF3176 0.23 -1.2 617,587 + GFF3176 0.61 -0.6 617,588 - GFF3176 0.61 +0.2 617,588 - GFF3176 0.61 -0.9 617,794 + -0.6 617,809 - -0.8 617,869 + -0.6 617,870 - -1.3 617,870 - +0.1 617,870 - +0.1 617,870 - -0.7 617,946 + -0.3 617,946 + +3.1 617,946 + -0.4 617,947 - +0.0 618,113 + GFF3177 0.80 -1.2 618,178 + +1.6 618,179 - +0.9 618,207 + -0.4 618,208 - -0.3 618,208 - -0.4 618,208 - -0.2 618,261 + +0.4 618,262 - -0.4 618,285 + +0.7 618,300 + +0.4 618,316 + +0.0 618,329 + -1.3 618,335 + +0.2 618,335 + +2.6 618,335 + -0.0 618,405 + -0.4 618,991 + GFF3178 0.17 +1.2 618,991 + GFF3178 0.17 -0.6 618,992 - GFF3178 0.18 -0.3 618,992 - GFF3178 0.18 -1.8 619,093 + GFF3178 0.21 +0.4 619,093 + GFF3178 0.21 -0.3 619,094 - GFF3178 0.21 +0.4 619,094 - GFF3178 0.21 +0.2 619,094 - GFF3178 0.21 -0.2
Or see this region's nucleotide sequence