Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF308 and GFF309 overlap by 4 nucleotides GFF309 and GFF310 overlap by 4 nucleotides GFF310 and GFF311 overlap by 4 nucleotides GFF311 and GFF312 overlap by 1 nucleotides
GFF308 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 341,331 to 342,062
GFF308
GFF309 - 3-hydroxydecanoyl-[ACP] dehydratase (EC 4.2.1.59), at 342,059 to 342,532
GFF309
GFF310 - FIG025233: SAM-dependent methyltransferases, at 342,529 to 343,305
GFF310
GFF311 - Polysaccharide deacetylase, at 343,302 to 344,144
GFF311
GFF312 - FIG021862: membrane protein, exporter, at 344,144 to 346,486
GFF312
Position (kb) 342
343
344 Strain fitness (log2 ratio) -2
-1
0
1
2 at 341.602 kb on + strand, within GFF308 at 341.602 kb on + strand, within GFF308 at 341.602 kb on + strand, within GFF308 at 341.602 kb on + strand, within GFF308 at 341.602 kb on + strand, within GFF308 at 341.602 kb on + strand, within GFF308 at 341.602 kb on + strand, within GFF308 at 341.602 kb on + strand, within GFF308 at 341.602 kb on + strand, within GFF308 at 341.603 kb on - strand, within GFF308 at 341.603 kb on - strand, within GFF308 at 341.603 kb on - strand, within GFF308 at 341.603 kb on - strand, within GFF308 at 341.603 kb on - strand, within GFF308 at 341.603 kb on - strand, within GFF308 at 341.603 kb on - strand, within GFF308 at 342.163 kb on - strand, within GFF309 at 342.163 kb on - strand, within GFF309 at 342.342 kb on + strand, within GFF309 at 342.342 kb on + strand, within GFF309 at 342.343 kb on - strand, within GFF309 at 342.851 kb on + strand, within GFF310 at 342.851 kb on + strand, within GFF310 at 342.852 kb on - strand, within GFF310 at 342.852 kb on - strand, within GFF310 at 342.852 kb on - strand, within GFF310 at 343.001 kb on + strand, within GFF310 at 343.229 kb on + strand at 343.229 kb on + strand at 343.229 kb on + strand at 343.230 kb on - strand at 343.230 kb on - strand at 343.377 kb on + strand at 343.377 kb on + strand at 343.377 kb on + strand at 343.377 kb on + strand at 343.378 kb on - strand at 343.378 kb on - strand at 343.378 kb on - strand at 343.378 kb on - strand at 343.378 kb on - strand at 343.378 kb on - strand at 343.378 kb on - strand at 343.378 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 341,602 + GFF308 0.37 -0.2 341,602 + GFF308 0.37 -0.5 341,602 + GFF308 0.37 -1.0 341,602 + GFF308 0.37 +0.3 341,602 + GFF308 0.37 -0.1 341,602 + GFF308 0.37 +0.2 341,602 + GFF308 0.37 -1.3 341,602 + GFF308 0.37 +2.0 341,602 + GFF308 0.37 -0.1 341,603 - GFF308 0.37 +0.6 341,603 - GFF308 0.37 -0.3 341,603 - GFF308 0.37 +0.6 341,603 - GFF308 0.37 -0.3 341,603 - GFF308 0.37 -0.2 341,603 - GFF308 0.37 -0.3 341,603 - GFF308 0.37 -0.1 342,163 - GFF309 0.22 -0.3 342,163 - GFF309 0.22 -0.1 342,342 + GFF309 0.60 +0.5 342,342 + GFF309 0.60 -0.7 342,343 - GFF309 0.60 -1.6 342,851 + GFF310 0.41 +1.8 342,851 + GFF310 0.41 -0.1 342,852 - GFF310 0.42 +0.5 342,852 - GFF310 0.42 +0.8 342,852 - GFF310 0.42 +0.2 343,001 + GFF310 0.61 +0.5 343,229 + -0.1 343,229 + -0.2 343,229 + -0.4 343,230 - +0.2 343,230 - -0.8 343,377 + +0.0 343,377 + -0.4 343,377 + -0.0 343,377 + +0.6 343,378 - -0.0 343,378 - -0.0 343,378 - -0.8 343,378 - -0.8 343,378 - +1.2 343,378 - -2.0 343,378 - -0.6 343,378 - +0.3
Or see this region's nucleotide sequence