Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF295 and GFF296 are separated by 10 nucleotides GFF296 and GFF297 are separated by 3 nucleotides GFF297 and GFF298 overlap by 4 nucleotides
GFF295 - Ribonuclease G, at 329,644 to 331,170
GFF295
GFF296 - Septum formation protein Maf, at 331,181 to 331,783
GFF296
GFF297 - FIG00978250: hypothetical protein, at 331,787 to 332,500
GFF297
GFF298 - 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase (EC 2.1.1.177), at 332,497 to 332,964
GFF298
Position (kb)
331
332 Strain fitness (log2 ratio)
-2
-1
0
1 at 330.305 kb on + strand, within GFF295 at 330.306 kb on - strand, within GFF295 at 330.306 kb on - strand, within GFF295 at 330.335 kb on + strand, within GFF295 at 330.335 kb on + strand, within GFF295 at 330.335 kb on + strand, within GFF295 at 330.335 kb on + strand, within GFF295 at 330.335 kb on + strand, within GFF295 at 330.335 kb on + strand, within GFF295 at 330.335 kb on + strand, within GFF295 at 330.335 kb on + strand, within GFF295 at 330.335 kb on + strand, within GFF295 at 330.336 kb on - strand, within GFF295 at 330.336 kb on - strand, within GFF295 at 330.336 kb on - strand, within GFF295 at 330.336 kb on - strand, within GFF295 at 330.336 kb on - strand, within GFF295 at 330.336 kb on - strand, within GFF295 at 330.336 kb on - strand, within GFF295 at 330.336 kb on - strand, within GFF295 at 330.336 kb on - strand, within GFF295 at 330.551 kb on + strand, within GFF295 at 330.551 kb on + strand, within GFF295 at 330.551 kb on + strand, within GFF295 at 330.552 kb on - strand, within GFF295 at 330.552 kb on - strand, within GFF295 at 331.049 kb on + strand at 331.050 kb on - strand at 331.050 kb on - strand at 331.186 kb on + strand at 331.187 kb on - strand at 331.187 kb on - strand at 331.187 kb on - strand at 331.187 kb on - strand at 331.238 kb on + strand at 331.251 kb on + strand, within GFF296 at 331.252 kb on - strand, within GFF296 at 331.302 kb on + strand, within GFF296 at 331.302 kb on + strand, within GFF296 at 331.302 kb on + strand, within GFF296 at 331.302 kb on + strand, within GFF296 at 331.302 kb on + strand, within GFF296 at 331.303 kb on - strand, within GFF296 at 331.303 kb on - strand, within GFF296 at 331.303 kb on - strand, within GFF296 at 331.303 kb on - strand, within GFF296 at 331.303 kb on - strand, within GFF296 at 331.303 kb on - strand, within GFF296 at 331.303 kb on - strand, within GFF296 at 331.303 kb on - strand, within GFF296 at 331.342 kb on - strand, within GFF296 at 331.632 kb on + strand, within GFF296 at 331.632 kb on + strand, within GFF296 at 331.632 kb on + strand, within GFF296 at 331.632 kb on + strand, within GFF296 at 331.632 kb on + strand, within GFF296 at 331.632 kb on + strand, within GFF296 at 331.633 kb on - strand, within GFF296 at 331.633 kb on - strand, within GFF296 at 331.633 kb on - strand, within GFF296 at 331.633 kb on - strand, within GFF296 at 331.633 kb on - strand, within GFF296 at 331.633 kb on - strand, within GFF296 at 331.633 kb on - strand, within GFF296 at 331.633 kb on - strand, within GFF296 at 331.703 kb on + strand, within GFF296 at 331.703 kb on + strand, within GFF296 at 331.703 kb on + strand, within GFF296 at 331.704 kb on - strand, within GFF296 at 331.704 kb on - strand, within GFF296 at 331.704 kb on - strand, within GFF296 at 331.704 kb on - strand, within GFF296 at 331.767 kb on + strand at 331.767 kb on + strand at 331.767 kb on + strand at 331.768 kb on - strand at 331.768 kb on - strand at 332.095 kb on + strand, within GFF297 at 332.095 kb on + strand, within GFF297 at 332.095 kb on + strand, within GFF297 at 332.095 kb on + strand, within GFF297 at 332.095 kb on + strand, within GFF297 at 332.096 kb on - strand, within GFF297 at 332.096 kb on - strand, within GFF297 at 332.096 kb on - strand, within GFF297 at 332.105 kb on + strand, within GFF297 at 332.106 kb on - strand, within GFF297 at 332.148 kb on + strand, within GFF297 at 332.148 kb on + strand, within GFF297 at 332.148 kb on + strand, within GFF297 at 332.148 kb on + strand, within GFF297 at 332.148 kb on + strand, within GFF297 at 332.148 kb on + strand, within GFF297 at 332.148 kb on + strand, within GFF297 at 332.148 kb on + strand, within GFF297 at 332.148 kb on + strand, within GFF297 at 332.148 kb on + strand, within GFF297 at 332.148 kb on + strand, within GFF297 at 332.149 kb on - strand, within GFF297 at 332.149 kb on - strand, within GFF297 at 332.149 kb on - strand, within GFF297 at 332.149 kb on - strand, within GFF297 at 332.150 kb on - strand, within GFF297 at 332.150 kb on - strand, within GFF297 at 332.454 kb on + strand at 332.454 kb on + strand at 332.454 kb on + strand at 332.510 kb on + strand at 332.510 kb on + strand at 332.510 kb on + strand at 332.510 kb on + strand at 332.510 kb on + strand at 332.511 kb on - strand at 332.511 kb on - strand at 332.511 kb on - strand at 332.511 kb on - strand at 332.543 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 330,305 + GFF295 0.43 +0.1 330,306 - GFF295 0.43 +0.2 330,306 - GFF295 0.43 +0.3 330,335 + GFF295 0.45 -0.1 330,335 + GFF295 0.45 -0.4 330,335 + GFF295 0.45 +0.0 330,335 + GFF295 0.45 -1.1 330,335 + GFF295 0.45 -1.8 330,335 + GFF295 0.45 +0.2 330,335 + GFF295 0.45 -1.0 330,335 + GFF295 0.45 +0.1 330,335 + GFF295 0.45 -0.3 330,336 - GFF295 0.45 -0.4 330,336 - GFF295 0.45 -0.1 330,336 - GFF295 0.45 +0.0 330,336 - GFF295 0.45 +0.1 330,336 - GFF295 0.45 -0.1 330,336 - GFF295 0.45 +1.4 330,336 - GFF295 0.45 +0.3 330,336 - GFF295 0.45 +0.5 330,336 - GFF295 0.45 -0.2 330,551 + GFF295 0.59 +0.2 330,551 + GFF295 0.59 -0.6 330,551 + GFF295 0.59 +0.1 330,552 - GFF295 0.59 +1.2 330,552 - GFF295 0.59 -0.3 331,049 + +1.7 331,050 - +0.9 331,050 - -1.0 331,186 + +0.7 331,187 - -1.0 331,187 - -0.7 331,187 - -0.3 331,187 - -1.4 331,238 + -0.7 331,251 + GFF296 0.12 +0.6 331,252 - GFF296 0.12 -0.5 331,302 + GFF296 0.20 +0.2 331,302 + GFF296 0.20 -1.1 331,302 + GFF296 0.20 +0.2 331,302 + GFF296 0.20 +0.2 331,302 + GFF296 0.20 -1.4 331,303 - GFF296 0.20 -0.6 331,303 - GFF296 0.20 -0.1 331,303 - GFF296 0.20 +0.5 331,303 - GFF296 0.20 -0.0 331,303 - GFF296 0.20 +0.1 331,303 - GFF296 0.20 -1.5 331,303 - GFF296 0.20 -0.8 331,303 - GFF296 0.20 -2.0 331,342 - GFF296 0.27 +0.5 331,632 + GFF296 0.75 -0.8 331,632 + GFF296 0.75 -1.0 331,632 + GFF296 0.75 -0.6 331,632 + GFF296 0.75 +0.6 331,632 + GFF296 0.75 +0.3 331,632 + GFF296 0.75 -0.0 331,633 - GFF296 0.75 -1.6 331,633 - GFF296 0.75 -0.2 331,633 - GFF296 0.75 -0.3 331,633 - GFF296 0.75 +0.4 331,633 - GFF296 0.75 -1.3 331,633 - GFF296 0.75 +0.3 331,633 - GFF296 0.75 +0.1 331,633 - GFF296 0.75 -1.7 331,703 + GFF296 0.87 -0.1 331,703 + GFF296 0.87 -0.5 331,703 + GFF296 0.87 -0.4 331,704 - GFF296 0.87 -1.9 331,704 - GFF296 0.87 -0.7 331,704 - GFF296 0.87 -2.7 331,704 - GFF296 0.87 -0.3 331,767 + +0.1 331,767 + +0.3 331,767 + +0.8 331,768 - -0.1 331,768 - -0.7 332,095 + GFF297 0.43 +0.3 332,095 + GFF297 0.43 +1.2 332,095 + GFF297 0.43 +0.1 332,095 + GFF297 0.43 -2.4 332,095 + GFF297 0.43 +0.2 332,096 - GFF297 0.43 -0.0 332,096 - GFF297 0.43 +0.2 332,096 - GFF297 0.43 -0.1 332,105 + GFF297 0.45 -0.1 332,106 - GFF297 0.45 +1.0 332,148 + GFF297 0.51 -1.8 332,148 + GFF297 0.51 -0.4 332,148 + GFF297 0.51 -0.2 332,148 + GFF297 0.51 -0.5 332,148 + GFF297 0.51 +0.1 332,148 + GFF297 0.51 +0.1 332,148 + GFF297 0.51 -1.1 332,148 + GFF297 0.51 +0.8 332,148 + GFF297 0.51 -0.4 332,148 + GFF297 0.51 -1.5 332,148 + GFF297 0.51 +0.4 332,149 - GFF297 0.51 +0.5 332,149 - GFF297 0.51 +1.1 332,149 - GFF297 0.51 +1.4 332,149 - GFF297 0.51 +0.6 332,150 - GFF297 0.51 +1.6 332,150 - GFF297 0.51 +0.3 332,454 + -0.5 332,454 + -0.3 332,454 + -0.0 332,510 + +0.9 332,510 + +0.0 332,510 + -1.3 332,510 + -0.2 332,510 + -0.6 332,511 - +1.0 332,511 - -0.9 332,511 - -0.2 332,511 - -0.2 332,543 + -0.3
Or see this region's nucleotide sequence