Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2957 and GFF2958 are separated by 4 nucleotides GFF2958 and GFF2959 overlap by 4 nucleotides GFF2959 and GFF2960 are separated by 64 nucleotides
GFF2957 - UPF0192 protein YfaS, at 381,130 to 385,842
GFF2957
GFF2958 - hypothetical protein, at 385,847 to 387,583
GFF2958
GFF2959 - Uncharacterized protein YfaP, at 387,580 to 388,395
GFF2959
GFF2960 - Acetyl-CoA synthetase (ADP-forming) alpha and beta chains, putative, at 388,460 to 389,023
GFF2960
Position (kb)
385
386
387
388 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 385.267 kb on + strand, within GFF2957 at 385.267 kb on + strand, within GFF2957 at 385.267 kb on + strand, within GFF2957 at 385.267 kb on + strand, within GFF2957 at 385.268 kb on - strand, within GFF2957 at 385.268 kb on - strand, within GFF2957 at 385.268 kb on - strand, within GFF2957 at 385.492 kb on + strand at 385.492 kb on + strand at 385.493 kb on - strand at 385.780 kb on + strand at 385.780 kb on + strand at 385.780 kb on + strand at 385.780 kb on + strand at 385.780 kb on + strand at 385.780 kb on + strand at 385.780 kb on + strand at 385.780 kb on + strand at 385.781 kb on - strand at 385.781 kb on - strand at 385.781 kb on - strand at 385.781 kb on - strand at 385.781 kb on - strand at 385.781 kb on - strand at 385.781 kb on - strand at 385.781 kb on - strand at 386.085 kb on + strand, within GFF2958 at 386.085 kb on + strand, within GFF2958 at 386.085 kb on + strand, within GFF2958 at 386.086 kb on - strand, within GFF2958 at 386.103 kb on - strand, within GFF2958 at 386.103 kb on - strand, within GFF2958 at 386.103 kb on - strand, within GFF2958 at 386.108 kb on + strand, within GFF2958 at 386.108 kb on + strand, within GFF2958 at 386.108 kb on + strand, within GFF2958 at 386.108 kb on + strand, within GFF2958 at 386.108 kb on + strand, within GFF2958 at 386.108 kb on + strand, within GFF2958 at 386.109 kb on - strand, within GFF2958 at 386.109 kb on - strand, within GFF2958 at 386.109 kb on - strand, within GFF2958 at 386.109 kb on - strand, within GFF2958 at 386.109 kb on - strand, within GFF2958 at 386.109 kb on - strand, within GFF2958 at 386.141 kb on + strand, within GFF2958 at 386.141 kb on + strand, within GFF2958 at 386.180 kb on + strand, within GFF2958 at 386.180 kb on + strand, within GFF2958 at 386.181 kb on - strand, within GFF2958 at 386.181 kb on - strand, within GFF2958 at 386.243 kb on + strand, within GFF2958 at 386.244 kb on - strand, within GFF2958 at 386.244 kb on - strand, within GFF2958 at 386.515 kb on + strand, within GFF2958 at 386.515 kb on + strand, within GFF2958 at 386.515 kb on + strand, within GFF2958 at 386.515 kb on + strand, within GFF2958 at 386.515 kb on - strand, within GFF2958 at 386.516 kb on - strand, within GFF2958 at 386.516 kb on - strand, within GFF2958 at 386.516 kb on - strand, within GFF2958 at 386.721 kb on - strand, within GFF2958 at 386.861 kb on + strand, within GFF2958 at 386.909 kb on + strand, within GFF2958 at 386.909 kb on + strand, within GFF2958 at 386.909 kb on + strand, within GFF2958 at 386.909 kb on + strand, within GFF2958 at 386.910 kb on - strand, within GFF2958 at 386.910 kb on - strand, within GFF2958 at 386.910 kb on - strand, within GFF2958 at 386.910 kb on - strand, within GFF2958 at 386.910 kb on - strand, within GFF2958 at 386.990 kb on + strand, within GFF2958 at 386.990 kb on + strand, within GFF2958 at 386.990 kb on + strand, within GFF2958 at 386.991 kb on - strand, within GFF2958 at 386.991 kb on - strand, within GFF2958 at 386.991 kb on - strand, within GFF2958 at 386.991 kb on - strand, within GFF2958 at 386.991 kb on - strand, within GFF2958 at 386.991 kb on - strand, within GFF2958 at 386.991 kb on - strand, within GFF2958 at 386.991 kb on - strand, within GFF2958 at 387.144 kb on - strand, within GFF2958 at 387.144 kb on - strand, within GFF2958 at 387.144 kb on - strand, within GFF2958 at 387.144 kb on - strand, within GFF2958 at 387.497 kb on + strand at 387.497 kb on + strand at 387.497 kb on + strand at 387.497 kb on + strand at 387.498 kb on - strand at 387.498 kb on - strand at 387.624 kb on + strand at 387.639 kb on + strand at 387.639 kb on + strand at 387.639 kb on + strand at 387.639 kb on + strand at 387.639 kb on + strand at 387.640 kb on - strand at 387.640 kb on - strand at 388.006 kb on + strand, within GFF2959 at 388.006 kb on + strand, within GFF2959 at 388.006 kb on + strand, within GFF2959 at 388.007 kb on - strand, within GFF2959 at 388.007 kb on - strand, within GFF2959 at 388.007 kb on - strand, within GFF2959 at 388.007 kb on - strand, within GFF2959 at 388.120 kb on + strand, within GFF2959 at 388.120 kb on + strand, within GFF2959 at 388.120 kb on + strand, within GFF2959 at 388.222 kb on + strand, within GFF2959 at 388.222 kb on + strand, within GFF2959 at 388.222 kb on + strand, within GFF2959 at 388.222 kb on + strand, within GFF2959 at 388.222 kb on + strand, within GFF2959 at 388.222 kb on + strand, within GFF2959 at 388.240 kb on + strand, within GFF2959 at 388.240 kb on + strand, within GFF2959 at 388.240 kb on + strand, within GFF2959 at 388.240 kb on + strand, within GFF2959 at 388.241 kb on - strand, within GFF2959
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 385,267 + GFF2957 0.88 +0.9 385,267 + GFF2957 0.88 -0.3 385,267 + GFF2957 0.88 -0.2 385,267 + GFF2957 0.88 -0.8 385,268 - GFF2957 0.88 -0.4 385,268 - GFF2957 0.88 -0.4 385,268 - GFF2957 0.88 -0.1 385,492 + +0.0 385,492 + -0.6 385,493 - -0.7 385,780 + +1.5 385,780 + -1.3 385,780 + -0.4 385,780 + +0.3 385,780 + +1.5 385,780 + +0.1 385,780 + +0.2 385,780 + -0.6 385,781 - -0.2 385,781 - +1.9 385,781 - +0.3 385,781 - -0.4 385,781 - -0.0 385,781 - +1.3 385,781 - +0.8 385,781 - -0.5 386,085 + GFF2958 0.14 +0.6 386,085 + GFF2958 0.14 -0.3 386,085 + GFF2958 0.14 -0.1 386,086 - GFF2958 0.14 +0.2 386,103 - GFF2958 0.15 +1.1 386,103 - GFF2958 0.15 -0.7 386,103 - GFF2958 0.15 -0.8 386,108 + GFF2958 0.15 -1.1 386,108 + GFF2958 0.15 +0.9 386,108 + GFF2958 0.15 +1.9 386,108 + GFF2958 0.15 -0.2 386,108 + GFF2958 0.15 +0.2 386,108 + GFF2958 0.15 -0.5 386,109 - GFF2958 0.15 +0.1 386,109 - GFF2958 0.15 +0.4 386,109 - GFF2958 0.15 +0.0 386,109 - GFF2958 0.15 -0.3 386,109 - GFF2958 0.15 -0.0 386,109 - GFF2958 0.15 +1.5 386,141 + GFF2958 0.17 -0.0 386,141 + GFF2958 0.17 -0.9 386,180 + GFF2958 0.19 -0.2 386,180 + GFF2958 0.19 -0.5 386,181 - GFF2958 0.19 -0.0 386,181 - GFF2958 0.19 +0.9 386,243 + GFF2958 0.23 +0.4 386,244 - GFF2958 0.23 -0.5 386,244 - GFF2958 0.23 -0.4 386,515 + GFF2958 0.38 +0.2 386,515 + GFF2958 0.38 +0.6 386,515 + GFF2958 0.38 -0.0 386,515 + GFF2958 0.38 +0.4 386,515 - GFF2958 0.38 -0.7 386,516 - GFF2958 0.39 +0.5 386,516 - GFF2958 0.39 -0.5 386,516 - GFF2958 0.39 -2.9 386,721 - GFF2958 0.50 -0.0 386,861 + GFF2958 0.58 -0.9 386,909 + GFF2958 0.61 +0.4 386,909 + GFF2958 0.61 +0.2 386,909 + GFF2958 0.61 +0.1 386,909 + GFF2958 0.61 -1.0 386,910 - GFF2958 0.61 +0.6 386,910 - GFF2958 0.61 -0.7 386,910 - GFF2958 0.61 +0.5 386,910 - GFF2958 0.61 -0.5 386,910 - GFF2958 0.61 +1.5 386,990 + GFF2958 0.66 -0.4 386,990 + GFF2958 0.66 -0.1 386,990 + GFF2958 0.66 -0.0 386,991 - GFF2958 0.66 +0.6 386,991 - GFF2958 0.66 +0.7 386,991 - GFF2958 0.66 -0.2 386,991 - GFF2958 0.66 -0.6 386,991 - GFF2958 0.66 -0.4 386,991 - GFF2958 0.66 -0.8 386,991 - GFF2958 0.66 -0.3 386,991 - GFF2958 0.66 +0.5 387,144 - GFF2958 0.75 +0.5 387,144 - GFF2958 0.75 -1.4 387,144 - GFF2958 0.75 +0.1 387,144 - GFF2958 0.75 -2.5 387,497 + -0.2 387,497 + -0.6 387,497 + -0.9 387,497 + +0.0 387,498 - -0.3 387,498 - +0.4 387,624 + -0.5 387,639 + +0.7 387,639 + -0.5 387,639 + -1.3 387,639 + -0.5 387,639 + -0.1 387,640 - -0.8 387,640 - -0.6 388,006 + GFF2959 0.52 -1.2 388,006 + GFF2959 0.52 -0.2 388,006 + GFF2959 0.52 +0.0 388,007 - GFF2959 0.52 +1.1 388,007 - GFF2959 0.52 +0.1 388,007 - GFF2959 0.52 +0.3 388,007 - GFF2959 0.52 +0.2 388,120 + GFF2959 0.66 +0.0 388,120 + GFF2959 0.66 -0.0 388,120 + GFF2959 0.66 +0.6 388,222 + GFF2959 0.79 +0.5 388,222 + GFF2959 0.79 -0.5 388,222 + GFF2959 0.79 -0.0 388,222 + GFF2959 0.79 +1.0 388,222 + GFF2959 0.79 -0.5 388,222 + GFF2959 0.79 -0.5 388,240 + GFF2959 0.81 -0.9 388,240 + GFF2959 0.81 -0.2 388,240 + GFF2959 0.81 -0.8 388,240 + GFF2959 0.81 -0.4 388,241 - GFF2959 0.81 -2.2
Or see this region's nucleotide sequence