Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF286 and GFF287 are separated by 2 nucleotides GFF287 and GFF288 are separated by 181 nucleotides GFF288 and GFF289 are separated by 387 nucleotides
GFF286 - Transcriptional regulator, LysR family, at 321,149 to 322,042
GFF286
GFF287 - hypothetical protein, at 322,045 to 323,301
GFF287
GFF288 - no description, at 323,483 to 323,812
GFF288
GFF289 - Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 1, at 324,200 to 325,045
GFF289
Position (kb) 322
323
324 Strain fitness (log2 ratio) -2
-1
0
1
2 at 321.052 kb on + strand at 321.053 kb on - strand at 321.053 kb on - strand at 321.053 kb on - strand at 321.053 kb on - strand at 321.053 kb on - strand at 321.053 kb on - strand at 321.053 kb on - strand at 321.085 kb on + strand at 321.085 kb on + strand at 321.085 kb on + strand at 321.085 kb on + strand at 321.085 kb on + strand at 321.085 kb on + strand at 321.086 kb on - strand at 321.086 kb on - strand at 321.086 kb on - strand at 321.170 kb on + strand at 321.170 kb on + strand at 321.171 kb on - strand at 321.171 kb on - strand at 321.171 kb on - strand at 321.224 kb on + strand at 321.224 kb on + strand at 321.224 kb on + strand at 321.224 kb on + strand at 321.224 kb on + strand at 321.224 kb on + strand at 321.224 kb on + strand at 321.225 kb on - strand at 321.225 kb on - strand at 321.225 kb on - strand at 321.225 kb on - strand at 321.225 kb on - strand at 321.225 kb on - strand at 321.225 kb on - strand at 321.225 kb on - strand at 321.225 kb on - strand at 321.282 kb on + strand, within GFF286 at 321.282 kb on + strand, within GFF286 at 321.282 kb on + strand, within GFF286 at 321.282 kb on + strand, within GFF286 at 321.283 kb on - strand, within GFF286 at 321.283 kb on - strand, within GFF286 at 321.314 kb on + strand, within GFF286 at 321.314 kb on + strand, within GFF286 at 321.314 kb on + strand, within GFF286 at 321.314 kb on + strand, within GFF286 at 321.488 kb on + strand, within GFF286 at 321.489 kb on - strand, within GFF286 at 321.489 kb on - strand, within GFF286 at 321.489 kb on - strand, within GFF286 at 321.490 kb on - strand, within GFF286 at 321.638 kb on + strand, within GFF286 at 321.638 kb on + strand, within GFF286 at 321.638 kb on + strand, within GFF286 at 321.639 kb on - strand, within GFF286 at 321.764 kb on + strand, within GFF286 at 321.765 kb on - strand, within GFF286 at 321.923 kb on + strand, within GFF286 at 321.923 kb on + strand, within GFF286 at 321.923 kb on + strand, within GFF286 at 321.924 kb on - strand, within GFF286 at 321.924 kb on - strand, within GFF286 at 321.924 kb on - strand, within GFF286 at 321.962 kb on + strand at 321.962 kb on + strand at 321.962 kb on + strand at 321.963 kb on - strand at 321.963 kb on - strand at 322.046 kb on + strand at 322.265 kb on + strand, within GFF287 at 322.265 kb on + strand, within GFF287 at 322.265 kb on + strand, within GFF287 at 322.265 kb on + strand, within GFF287 at 322.265 kb on + strand, within GFF287 at 322.265 kb on + strand, within GFF287 at 322.266 kb on - strand, within GFF287 at 322.266 kb on - strand, within GFF287 at 322.266 kb on - strand, within GFF287 at 322.266 kb on - strand, within GFF287 at 322.266 kb on - strand, within GFF287 at 322.266 kb on - strand, within GFF287 at 322.266 kb on - strand, within GFF287 at 322.266 kb on - strand, within GFF287 at 322.266 kb on - strand, within GFF287 at 322.266 kb on - strand, within GFF287 at 322.442 kb on - strand, within GFF287 at 322.568 kb on + strand, within GFF287 at 322.569 kb on - strand, within GFF287 at 322.569 kb on - strand, within GFF287 at 322.719 kb on - strand, within GFF287 at 323.196 kb on - strand at 323.196 kb on - strand at 323.196 kb on - strand at 323.296 kb on + strand at 323.360 kb on + strand at 323.360 kb on + strand at 323.360 kb on + strand at 323.360 kb on + strand at 323.360 kb on + strand at 323.360 kb on + strand at 323.360 kb on + strand at 323.360 kb on + strand at 323.361 kb on - strand at 323.361 kb on - strand at 323.361 kb on - strand at 323.361 kb on - strand at 323.361 kb on - strand at 323.377 kb on - strand at 323.386 kb on - strand at 323.387 kb on - strand at 323.410 kb on + strand at 323.421 kb on + strand at 323.479 kb on + strand at 323.479 kb on + strand at 323.479 kb on + strand at 323.479 kb on + strand at 323.479 kb on + strand at 323.479 kb on + strand at 323.509 kb on + strand at 323.509 kb on + strand at 323.509 kb on + strand at 323.510 kb on - strand at 323.510 kb on - strand at 323.510 kb on - strand at 323.510 kb on - strand at 323.510 kb on - strand at 323.510 kb on - strand at 323.510 kb on - strand at 323.510 kb on - strand at 323.510 kb on - strand at 323.526 kb on + strand, within GFF288 at 323.526 kb on + strand, within GFF288 at 323.526 kb on + strand, within GFF288 at 323.526 kb on + strand, within GFF288 at 323.526 kb on + strand, within GFF288 at 323.526 kb on + strand, within GFF288 at 323.526 kb on + strand, within GFF288 at 323.526 kb on + strand, within GFF288 at 323.526 kb on + strand, within GFF288 at 323.526 kb on + strand, within GFF288 at 323.526 kb on + strand, within GFF288 at 323.526 kb on + strand, within GFF288 at 323.527 kb on - strand, within GFF288 at 323.527 kb on - strand, within GFF288 at 323.527 kb on - strand, within GFF288 at 323.527 kb on - strand, within GFF288 at 323.527 kb on - strand, within GFF288 at 323.527 kb on - strand, within GFF288 at 323.527 kb on - strand, within GFF288 at 323.527 kb on - strand, within GFF288 at 323.527 kb on - strand, within GFF288 at 323.527 kb on - strand, within GFF288 at 323.527 kb on - strand, within GFF288 at 323.538 kb on + strand, within GFF288 at 323.538 kb on + strand, within GFF288 at 323.538 kb on + strand, within GFF288 at 323.538 kb on + strand, within GFF288 at 323.538 kb on + strand, within GFF288 at 323.538 kb on + strand, within GFF288 at 323.538 kb on + strand, within GFF288 at 323.538 kb on + strand, within GFF288 at 323.538 kb on + strand, within GFF288 at 323.538 kb on + strand, within GFF288 at 323.538 kb on + strand, within GFF288 at 323.538 kb on + strand, within GFF288 at 323.539 kb on - strand, within GFF288 at 323.539 kb on - strand, within GFF288 at 323.539 kb on - strand, within GFF288 at 323.539 kb on - strand, within GFF288 at 323.539 kb on - strand, within GFF288 at 323.539 kb on - strand, within GFF288 at 323.539 kb on - strand, within GFF288 at 323.539 kb on - strand, within GFF288 at 323.539 kb on - strand, within GFF288 at 323.539 kb on - strand, within GFF288 at 323.689 kb on + strand, within GFF288 at 323.689 kb on + strand, within GFF288 at 323.690 kb on - strand, within GFF288 at 323.690 kb on - strand, within GFF288 at 323.690 kb on - strand, within GFF288 at 323.691 kb on + strand, within GFF288 at 323.691 kb on + strand, within GFF288 at 323.691 kb on + strand, within GFF288 at 323.691 kb on + strand, within GFF288 at 323.691 kb on + strand, within GFF288 at 323.691 kb on + strand, within GFF288 at 323.691 kb on + strand, within GFF288 at 323.691 kb on + strand, within GFF288 at 323.691 kb on + strand, within GFF288 at 323.691 kb on + strand, within GFF288 at 323.692 kb on - strand, within GFF288 at 323.692 kb on - strand, within GFF288 at 323.692 kb on - strand, within GFF288 at 323.692 kb on - strand, within GFF288 at 323.692 kb on - strand, within GFF288 at 323.692 kb on - strand, within GFF288 at 323.812 kb on + strand at 323.812 kb on + strand at 323.812 kb on + strand at 323.812 kb on + strand at 323.812 kb on + strand at 323.812 kb on + strand at 323.812 kb on + strand at 323.812 kb on + strand at 323.812 kb on + strand at 323.813 kb on - strand at 323.813 kb on - strand at 323.813 kb on - strand at 323.813 kb on - strand at 323.813 kb on - strand at 323.813 kb on - strand at 323.813 kb on - strand at 323.813 kb on - strand at 323.813 kb on - strand at 324.017 kb on + strand at 324.017 kb on + strand at 324.017 kb on + strand at 324.018 kb on - strand at 324.079 kb on - strand at 324.079 kb on - strand at 324.079 kb on - strand at 324.079 kb on - strand at 324.196 kb on - strand at 324.204 kb on - strand at 324.204 kb on - strand at 324.204 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 321,052 + +0.6 321,053 - -2.0 321,053 - +1.6 321,053 - +1.4 321,053 - -0.3 321,053 - -0.4 321,053 - +0.5 321,053 - +0.3 321,085 + +1.0 321,085 + +0.2 321,085 + +0.5 321,085 + +0.6 321,085 + +0.4 321,085 + -0.7 321,086 - +1.2 321,086 - -0.0 321,086 - -1.0 321,170 + +0.1 321,170 + +0.8 321,171 - +0.9 321,171 - -1.8 321,171 - -1.5 321,224 + +0.3 321,224 + -0.4 321,224 + +1.1 321,224 + -0.1 321,224 + +0.6 321,224 + +0.0 321,224 + -0.1 321,225 - -1.3 321,225 - +0.3 321,225 - -0.1 321,225 - +0.2 321,225 - -0.2 321,225 - -0.6 321,225 - +0.9 321,225 - +0.4 321,225 - +0.9 321,282 + GFF286 0.15 -0.2 321,282 + GFF286 0.15 +1.2 321,282 + GFF286 0.15 +1.2 321,282 + GFF286 0.15 -0.2 321,283 - GFF286 0.15 -1.3 321,283 - GFF286 0.15 +0.0 321,314 + GFF286 0.18 -0.5 321,314 + GFF286 0.18 +1.4 321,314 + GFF286 0.18 +0.8 321,314 + GFF286 0.18 -0.3 321,488 + GFF286 0.38 -0.5 321,489 - GFF286 0.38 -0.2 321,489 - GFF286 0.38 -0.6 321,489 - GFF286 0.38 +0.1 321,490 - GFF286 0.38 +0.9 321,638 + GFF286 0.55 -0.3 321,638 + GFF286 0.55 -0.3 321,638 + GFF286 0.55 +0.0 321,639 - GFF286 0.55 +0.4 321,764 + GFF286 0.69 +0.1 321,765 - GFF286 0.69 -0.1 321,923 + GFF286 0.87 -2.0 321,923 + GFF286 0.87 +0.8 321,923 + GFF286 0.87 -0.1 321,924 - GFF286 0.87 -1.6 321,924 - GFF286 0.87 +0.2 321,924 - GFF286 0.87 -0.8 321,962 + +1.9 321,962 + -0.7 321,962 + -0.4 321,963 - -0.5 321,963 - -0.6 322,046 + -0.8 322,265 + GFF287 0.18 +0.1 322,265 + GFF287 0.18 -1.0 322,265 + GFF287 0.18 -0.0 322,265 + GFF287 0.18 +0.2 322,265 + GFF287 0.18 +2.5 322,265 + GFF287 0.18 -1.3 322,266 - GFF287 0.18 -0.4 322,266 - GFF287 0.18 -0.0 322,266 - GFF287 0.18 -1.5 322,266 - GFF287 0.18 -1.6 322,266 - GFF287 0.18 -0.2 322,266 - GFF287 0.18 -0.5 322,266 - GFF287 0.18 +0.2 322,266 - GFF287 0.18 -0.6 322,266 - GFF287 0.18 -2.5 322,266 - GFF287 0.18 -0.8 322,442 - GFF287 0.32 -0.1 322,568 + GFF287 0.42 -0.7 322,569 - GFF287 0.42 +0.3 322,569 - GFF287 0.42 +0.1 322,719 - GFF287 0.54 -0.2 323,196 - -0.8 323,196 - -0.7 323,196 - -0.6 323,296 + +0.5 323,360 + -0.2 323,360 + -0.2 323,360 + +0.2 323,360 + -0.3 323,360 + -1.8 323,360 + +0.2 323,360 + -0.1 323,360 + +0.7 323,361 - -0.7 323,361 - -1.1 323,361 - +0.2 323,361 - -2.3 323,361 - -0.6 323,377 - -1.4 323,386 - -0.3 323,387 - -0.4 323,410 + -0.2 323,421 + -0.7 323,479 + +0.2 323,479 + +1.2 323,479 + -1.9 323,479 + -1.0 323,479 + -1.4 323,479 + +0.4 323,509 + +0.6 323,509 + +1.9 323,509 + -1.0 323,510 - +0.6 323,510 - +1.9 323,510 - -0.8 323,510 - +0.7 323,510 - -1.1 323,510 - -0.1 323,510 - +0.6 323,510 - +0.1 323,510 - +0.7 323,526 + GFF288 0.13 -0.4 323,526 + GFF288 0.13 -0.6 323,526 + GFF288 0.13 +1.9 323,526 + GFF288 0.13 -2.7 323,526 + GFF288 0.13 +0.4 323,526 + GFF288 0.13 -0.4 323,526 + GFF288 0.13 -0.0 323,526 + GFF288 0.13 -0.5 323,526 + GFF288 0.13 +0.7 323,526 + GFF288 0.13 +0.1 323,526 + GFF288 0.13 +0.8 323,526 + GFF288 0.13 +0.2 323,527 - GFF288 0.13 +0.3 323,527 - GFF288 0.13 -0.7 323,527 - GFF288 0.13 -0.1 323,527 - GFF288 0.13 +0.0 323,527 - GFF288 0.13 +0.2 323,527 - GFF288 0.13 +0.4 323,527 - GFF288 0.13 -0.6 323,527 - GFF288 0.13 -1.5 323,527 - GFF288 0.13 -0.7 323,527 - GFF288 0.13 -0.4 323,527 - GFF288 0.13 +0.1 323,538 + GFF288 0.17 +0.2 323,538 + GFF288 0.17 +0.0 323,538 + GFF288 0.17 +0.5 323,538 + GFF288 0.17 +0.2 323,538 + GFF288 0.17 -0.2 323,538 + GFF288 0.17 +1.0 323,538 + GFF288 0.17 +0.2 323,538 + GFF288 0.17 +1.0 323,538 + GFF288 0.17 +1.4 323,538 + GFF288 0.17 -0.9 323,538 + GFF288 0.17 -0.4 323,538 + GFF288 0.17 +0.2 323,539 - GFF288 0.17 -0.2 323,539 - GFF288 0.17 -1.3 323,539 - GFF288 0.17 +1.0 323,539 - GFF288 0.17 -0.4 323,539 - GFF288 0.17 -1.3 323,539 - GFF288 0.17 +0.1 323,539 - GFF288 0.17 +0.2 323,539 - GFF288 0.17 -0.7 323,539 - GFF288 0.17 +0.2 323,539 - GFF288 0.17 -1.1 323,689 + GFF288 0.62 -1.4 323,689 + GFF288 0.62 -0.5 323,690 - GFF288 0.63 +0.4 323,690 - GFF288 0.63 -1.6 323,690 - GFF288 0.63 -0.5 323,691 + GFF288 0.63 +0.1 323,691 + GFF288 0.63 -1.5 323,691 + GFF288 0.63 -0.1 323,691 + GFF288 0.63 -0.1 323,691 + GFF288 0.63 +2.7 323,691 + GFF288 0.63 +0.2 323,691 + GFF288 0.63 +0.3 323,691 + GFF288 0.63 -0.3 323,691 + GFF288 0.63 -0.6 323,691 + GFF288 0.63 +0.1 323,692 - GFF288 0.63 -0.7 323,692 - GFF288 0.63 -0.4 323,692 - GFF288 0.63 -0.4 323,692 - GFF288 0.63 +0.1 323,692 - GFF288 0.63 -0.3 323,692 - GFF288 0.63 -0.6 323,812 + +1.8 323,812 + -0.1 323,812 + -0.1 323,812 + -0.1 323,812 + +0.2 323,812 + +1.1 323,812 + +0.2 323,812 + +0.5 323,812 + -0.3 323,813 - -0.5 323,813 - +0.1 323,813 - -1.1 323,813 - +0.4 323,813 - -0.1 323,813 - +0.0 323,813 - +0.4 323,813 - +0.2 323,813 - -0.2 324,017 + -0.2 324,017 + +0.2 324,017 + +1.2 324,018 - -0.8 324,079 - +0.2 324,079 - -1.0 324,079 - -0.3 324,079 - -0.3 324,196 - -1.4 324,204 - +0.3 324,204 - +0.2 324,204 - +0.2
Or see this region's nucleotide sequence