Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2662 and GFF2663 are separated by 73 nucleotides GFF2663 and GFF2664 overlap by 4 nucleotides GFF2664 and GFF2665 are separated by 18 nucleotides
GFF2662 - Deacetylases, including yeast histone deacetylase and acetoin utilization protein, at 84,553 to 85,506
GFF2662
GFF2663 - FIG022979: MoxR-like ATPases, at 85,580 to 86,500
GFF2663
GFF2664 - FIG002343: hypothetical protein, at 86,497 to 87,474
GFF2664
GFF2665 - FIG001454: Transglutaminase-like enzymes, putative cysteine proteases, at 87,493 to 89,514
GFF2665
Position (kb)
85
86
87 Strain fitness (log2 ratio)
-2
-1
0
1 at 84.615 kb on - strand at 84.845 kb on + strand, within GFF2662 at 84.899 kb on + strand, within GFF2662 at 84.899 kb on + strand, within GFF2662 at 84.900 kb on - strand, within GFF2662 at 84.905 kb on + strand, within GFF2662 at 84.905 kb on + strand, within GFF2662 at 84.906 kb on - strand, within GFF2662 at 84.906 kb on - strand, within GFF2662 at 84.906 kb on - strand, within GFF2662 at 84.906 kb on - strand, within GFF2662 at 84.906 kb on - strand, within GFF2662 at 85.490 kb on + strand at 85.490 kb on + strand at 85.491 kb on - strand at 85.491 kb on - strand at 85.491 kb on - strand at 85.491 kb on - strand at 85.491 kb on - strand at 85.519 kb on + strand at 85.519 kb on + strand at 85.520 kb on - strand at 85.520 kb on - strand at 85.551 kb on + strand at 85.552 kb on - strand at 85.552 kb on - strand at 85.552 kb on - strand at 85.552 kb on - strand at 85.552 kb on - strand at 85.573 kb on + strand at 85.574 kb on - strand at 85.574 kb on - strand at 85.574 kb on - strand at 85.579 kb on + strand at 85.579 kb on + strand at 85.579 kb on + strand at 85.579 kb on + strand at 85.580 kb on - strand at 85.586 kb on + strand at 85.599 kb on + strand at 85.599 kb on + strand at 85.600 kb on - strand at 85.600 kb on - strand at 85.611 kb on + strand at 85.612 kb on - strand at 85.621 kb on + strand at 85.621 kb on + strand at 85.621 kb on + strand at 85.622 kb on - strand at 85.826 kb on + strand, within GFF2663 at 85.826 kb on + strand, within GFF2663 at 85.826 kb on + strand, within GFF2663 at 85.826 kb on + strand, within GFF2663 at 85.826 kb on + strand, within GFF2663 at 85.827 kb on - strand, within GFF2663 at 85.827 kb on - strand, within GFF2663 at 86.246 kb on + strand, within GFF2663 at 86.246 kb on + strand, within GFF2663 at 86.247 kb on - strand, within GFF2663 at 86.247 kb on - strand, within GFF2663 at 86.247 kb on - strand, within GFF2663 at 86.247 kb on - strand, within GFF2663 at 86.247 kb on - strand, within GFF2663 at 86.369 kb on + strand, within GFF2663 at 86.369 kb on + strand, within GFF2663 at 86.369 kb on + strand, within GFF2663 at 86.370 kb on - strand, within GFF2663 at 86.370 kb on - strand, within GFF2663 at 86.370 kb on - strand, within GFF2663 at 86.370 kb on - strand, within GFF2663 at 86.593 kb on + strand at 86.593 kb on + strand at 86.593 kb on + strand at 86.593 kb on + strand at 86.594 kb on - strand at 86.594 kb on - strand at 86.666 kb on - strand, within GFF2664 at 86.684 kb on - strand, within GFF2664 at 86.684 kb on - strand, within GFF2664 at 86.848 kb on + strand, within GFF2664 at 86.849 kb on - strand, within GFF2664 at 87.053 kb on - strand, within GFF2664
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 84,615 - +0.0 84,845 + GFF2662 0.31 -0.1 84,899 + GFF2662 0.36 -0.3 84,899 + GFF2662 0.36 -0.1 84,900 - GFF2662 0.36 +1.5 84,905 + GFF2662 0.37 -0.6 84,905 + GFF2662 0.37 -1.0 84,906 - GFF2662 0.37 -1.4 84,906 - GFF2662 0.37 +0.0 84,906 - GFF2662 0.37 -0.4 84,906 - GFF2662 0.37 +0.0 84,906 - GFF2662 0.37 +0.3 85,490 + +0.1 85,490 + -1.1 85,491 - -0.5 85,491 - -0.7 85,491 - -0.8 85,491 - -0.8 85,491 - -0.5 85,519 + -0.7 85,519 + -0.7 85,520 - -0.1 85,520 - -0.0 85,551 + -0.7 85,552 - -0.5 85,552 - +0.1 85,552 - -0.8 85,552 - -0.5 85,552 - +0.6 85,573 + -0.2 85,574 - +1.3 85,574 - +0.5 85,574 - -0.6 85,579 + +1.1 85,579 + -0.4 85,579 + +0.2 85,579 + +0.1 85,580 - -0.6 85,586 + -0.0 85,599 + +0.5 85,599 + +0.0 85,600 - -0.6 85,600 - -0.2 85,611 + +0.2 85,612 - -1.1 85,621 + -0.4 85,621 + -0.7 85,621 + +0.8 85,622 - -0.4 85,826 + GFF2663 0.27 +0.2 85,826 + GFF2663 0.27 -0.1 85,826 + GFF2663 0.27 +0.2 85,826 + GFF2663 0.27 -0.6 85,826 + GFF2663 0.27 -0.4 85,827 - GFF2663 0.27 +0.3 85,827 - GFF2663 0.27 -0.1 86,246 + GFF2663 0.72 +0.5 86,246 + GFF2663 0.72 +1.1 86,247 - GFF2663 0.72 -0.6 86,247 - GFF2663 0.72 -0.4 86,247 - GFF2663 0.72 -0.5 86,247 - GFF2663 0.72 -0.3 86,247 - GFF2663 0.72 +0.9 86,369 + GFF2663 0.86 +0.0 86,369 + GFF2663 0.86 -0.6 86,369 + GFF2663 0.86 +1.1 86,370 - GFF2663 0.86 -0.5 86,370 - GFF2663 0.86 -0.3 86,370 - GFF2663 0.86 +0.2 86,370 - GFF2663 0.86 -2.0 86,593 + -1.7 86,593 + -1.9 86,593 + +0.5 86,593 + -0.0 86,594 - +0.8 86,594 - +0.2 86,666 - GFF2664 0.17 -1.2 86,684 - GFF2664 0.19 -0.7 86,684 - GFF2664 0.19 -0.1 86,848 + GFF2664 0.36 -0.8 86,849 - GFF2664 0.36 -0.2 87,053 - GFF2664 0.57 -0.4
Or see this region's nucleotide sequence