Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2404 and GFF2405 are separated by 152 nucleotides GFF2405 and GFF2406 are separated by 21 nucleotides
GFF2404 - Hypothetical protein with Rieske (2Fe-2S) region, at 456,540 to 457,622
GFF2404
GFF2405 - Peptide-methionine (R)-S-oxide reductase MsrB (EC 1.8.4.12), at 457,775 to 458,284
GFF2405
GFF2406 - DipZ protein, at 458,306 to 460,159
GFF2406
Position (kb) 457
458
459 Strain fitness (log2 ratio) -2
-1
0
1
2 at 456.846 kb on + strand, within GFF2404 at 456.847 kb on - strand, within GFF2404 at 457.173 kb on + strand, within GFF2404 at 457.173 kb on + strand, within GFF2404 at 457.174 kb on - strand, within GFF2404 at 457.212 kb on + strand, within GFF2404 at 457.233 kb on + strand, within GFF2404 at 457.233 kb on + strand, within GFF2404 at 457.354 kb on + strand, within GFF2404 at 457.354 kb on + strand, within GFF2404 at 457.354 kb on + strand, within GFF2404 at 457.355 kb on - strand, within GFF2404 at 457.355 kb on - strand, within GFF2404 at 457.355 kb on - strand, within GFF2404 at 457.355 kb on - strand, within GFF2404 at 457.449 kb on + strand, within GFF2404 at 457.449 kb on + strand, within GFF2404 at 457.449 kb on + strand, within GFF2404 at 457.450 kb on - strand, within GFF2404 at 457.450 kb on - strand, within GFF2404 at 457.450 kb on - strand, within GFF2404 at 457.710 kb on + strand at 457.717 kb on + strand at 457.717 kb on + strand at 457.718 kb on - strand at 457.718 kb on - strand at 457.727 kb on + strand at 457.727 kb on + strand at 457.727 kb on + strand at 457.727 kb on + strand at 457.727 kb on + strand at 457.727 kb on + strand at 457.727 kb on + strand at 457.727 kb on + strand at 457.727 kb on + strand at 457.727 kb on + strand at 457.727 kb on + strand at 457.727 kb on + strand at 457.728 kb on - strand at 457.728 kb on - strand at 457.728 kb on - strand at 457.728 kb on - strand at 457.728 kb on - strand at 457.729 kb on + strand at 457.729 kb on + strand at 457.730 kb on - strand at 457.730 kb on - strand at 457.731 kb on + strand at 457.731 kb on + strand at 457.731 kb on + strand at 457.731 kb on + strand at 457.731 kb on + strand at 457.732 kb on - strand at 457.732 kb on - strand at 457.946 kb on + strand, within GFF2405 at 457.947 kb on - strand, within GFF2405 at 457.947 kb on - strand, within GFF2405 at 458.237 kb on + strand at 458.328 kb on - strand at 458.810 kb on + strand, within GFF2406 at 458.853 kb on + strand, within GFF2406 at 458.854 kb on - strand, within GFF2406 at 458.854 kb on - strand, within GFF2406
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 456,846 + GFF2404 0.28 -0.6 456,847 - GFF2404 0.28 +0.2 457,173 + GFF2404 0.58 -0.7 457,173 + GFF2404 0.58 -0.6 457,174 - GFF2404 0.59 -0.2 457,212 + GFF2404 0.62 +0.1 457,233 + GFF2404 0.64 -0.1 457,233 + GFF2404 0.64 -1.4 457,354 + GFF2404 0.75 -0.5 457,354 + GFF2404 0.75 -0.0 457,354 + GFF2404 0.75 +0.4 457,355 - GFF2404 0.75 -0.1 457,355 - GFF2404 0.75 -0.3 457,355 - GFF2404 0.75 +0.8 457,355 - GFF2404 0.75 +1.7 457,449 + GFF2404 0.84 +0.4 457,449 + GFF2404 0.84 +1.1 457,449 + GFF2404 0.84 -0.7 457,450 - GFF2404 0.84 -0.9 457,450 - GFF2404 0.84 -1.8 457,450 - GFF2404 0.84 -0.4 457,710 + -0.2 457,717 + +0.1 457,717 + -0.7 457,718 - +0.1 457,718 - +0.6 457,727 + -0.1 457,727 + -0.9 457,727 + +2.1 457,727 + +0.1 457,727 + -1.3 457,727 + -0.0 457,727 + -0.1 457,727 + +1.1 457,727 + +0.3 457,727 + +0.6 457,727 + +0.1 457,727 + -0.7 457,728 - -1.0 457,728 - -0.5 457,728 - +0.1 457,728 - -0.0 457,728 - -0.1 457,729 + +0.2 457,729 + +0.3 457,730 - -0.1 457,730 - -0.3 457,731 + -0.6 457,731 + -0.2 457,731 + -0.8 457,731 + +0.7 457,731 + +0.3 457,732 - -0.3 457,732 - -0.1 457,946 + GFF2405 0.34 -0.3 457,947 - GFF2405 0.34 -2.1 457,947 - GFF2405 0.34 -0.4 458,237 + +0.0 458,328 - +0.2 458,810 + GFF2406 0.27 -0.2 458,853 + GFF2406 0.30 -0.2 458,854 - GFF2406 0.30 +0.1 458,854 - GFF2406 0.30 +0.1
Or see this region's nucleotide sequence