Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2318 and GFF2319 are separated by 78 nucleotides GFF2319 and GFF2320 are separated by 29 nucleotides GFF2320 and GFF2321 are separated by 43 nucleotides GFF2321 and GFF2322 are separated by 6 nucleotides
GFF2318 - Transcriptional regulator ArgP, LysR family, at 370,540 to 371,454
GFF2318
GFF2319 - Arginine exporter protein ArgO, at 371,533 to 372,168
GFF2319
GFF2320 - 16S rRNA (guanine(966)-N(2))-methyltransferase (EC 2.1.1.171), at 372,198 to 372,845
GFF2320
GFF2321 - Phosphopantetheine adenylyltransferase (EC 2.7.7.3), at 372,889 to 373,392
GFF2321
GFF2322 - Allophanate hydrolase 2 subunit 1 (EC 3.5.1.54), at 373,399 to 374,061
GFF2322
Position (kb)
372
373 Strain fitness (log2 ratio)
-1
0
1
2
3 at 371.445 kb on - strand at 371.445 kb on - strand at 371.445 kb on - strand at 371.445 kb on - strand at 371.469 kb on - strand at 371.504 kb on + strand at 371.505 kb on - strand at 371.543 kb on + strand at 371.543 kb on + strand at 371.543 kb on + strand at 371.543 kb on + strand at 371.544 kb on - strand at 371.544 kb on - strand at 371.620 kb on + strand, within GFF2319 at 371.620 kb on + strand, within GFF2319 at 371.621 kb on - strand, within GFF2319 at 371.621 kb on - strand, within GFF2319 at 371.621 kb on - strand, within GFF2319 at 371.621 kb on - strand, within GFF2319 at 371.780 kb on + strand, within GFF2319 at 371.780 kb on + strand, within GFF2319 at 371.781 kb on - strand, within GFF2319 at 371.781 kb on - strand, within GFF2319 at 371.781 kb on - strand, within GFF2319 at 371.794 kb on + strand, within GFF2319 at 371.794 kb on + strand, within GFF2319 at 371.794 kb on + strand, within GFF2319 at 371.794 kb on + strand, within GFF2319 at 371.794 kb on + strand, within GFF2319 at 371.794 kb on + strand, within GFF2319 at 371.794 kb on + strand, within GFF2319 at 371.794 kb on + strand, within GFF2319 at 371.795 kb on - strand, within GFF2319 at 371.795 kb on - strand, within GFF2319 at 371.795 kb on - strand, within GFF2319 at 371.795 kb on - strand, within GFF2319 at 371.795 kb on - strand, within GFF2319 at 371.795 kb on - strand, within GFF2319 at 371.795 kb on - strand, within GFF2319 at 371.795 kb on - strand, within GFF2319 at 371.923 kb on + strand, within GFF2319 at 371.923 kb on + strand, within GFF2319 at 371.923 kb on + strand, within GFF2319 at 371.923 kb on + strand, within GFF2319 at 371.924 kb on - strand, within GFF2319 at 371.924 kb on - strand, within GFF2319 at 371.924 kb on - strand, within GFF2319 at 372.186 kb on + strand at 372.187 kb on - strand at 372.187 kb on - strand at 372.187 kb on - strand at 372.663 kb on + strand, within GFF2320 at 372.663 kb on + strand, within GFF2320 at 372.664 kb on - strand, within GFF2320 at 372.718 kb on - strand, within GFF2320 at 372.718 kb on - strand, within GFF2320 at 372.718 kb on - strand, within GFF2320 at 372.718 kb on - strand, within GFF2320 at 372.843 kb on + strand at 372.843 kb on + strand at 372.843 kb on + strand at 372.844 kb on - strand at 372.844 kb on - strand at 372.844 kb on - strand at 373.390 kb on + strand at 373.390 kb on + strand at 373.390 kb on + strand at 373.391 kb on - strand at 373.391 kb on - strand at 373.391 kb on - strand at 373.391 kb on - strand at 373.602 kb on + strand, within GFF2322 at 373.602 kb on + strand, within GFF2322 at 373.603 kb on - strand, within GFF2322 at 373.687 kb on + strand, within GFF2322 at 373.687 kb on + strand, within GFF2322 at 373.687 kb on + strand, within GFF2322 at 373.687 kb on + strand, within GFF2322 at 373.687 kb on + strand, within GFF2322 at 373.688 kb on - strand, within GFF2322 at 373.688 kb on - strand, within GFF2322 at 373.780 kb on + strand, within GFF2322 at 373.780 kb on + strand, within GFF2322 at 373.780 kb on + strand, within GFF2322 at 373.780 kb on + strand, within GFF2322 at 373.780 kb on + strand, within GFF2322 at 373.780 kb on + strand, within GFF2322 at 373.780 kb on + strand, within GFF2322 at 373.781 kb on - strand, within GFF2322 at 373.781 kb on - strand, within GFF2322 at 373.781 kb on - strand, within GFF2322 at 373.781 kb on - strand, within GFF2322 at 373.781 kb on - strand, within GFF2322 at 373.789 kb on + strand, within GFF2322 at 373.790 kb on - strand, within GFF2322 at 373.790 kb on - strand, within GFF2322 at 373.790 kb on - strand, within GFF2322 at 373.790 kb on - strand, within GFF2322 at 373.790 kb on - strand, within GFF2322
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 371,445 - -0.4 371,445 - -1.2 371,445 - +1.0 371,445 - -0.4 371,469 - -0.2 371,504 + -1.5 371,505 - -0.1 371,543 + +2.1 371,543 + +0.4 371,543 + +0.7 371,543 + -0.5 371,544 - -0.2 371,544 - +0.3 371,620 + GFF2319 0.14 +0.2 371,620 + GFF2319 0.14 -0.4 371,621 - GFF2319 0.14 -0.2 371,621 - GFF2319 0.14 +0.1 371,621 - GFF2319 0.14 -1.2 371,621 - GFF2319 0.14 +0.2 371,780 + GFF2319 0.39 +0.4 371,780 + GFF2319 0.39 +0.7 371,781 - GFF2319 0.39 +0.2 371,781 - GFF2319 0.39 -0.1 371,781 - GFF2319 0.39 -0.9 371,794 + GFF2319 0.41 +0.8 371,794 + GFF2319 0.41 -0.3 371,794 + GFF2319 0.41 +0.0 371,794 + GFF2319 0.41 +0.1 371,794 + GFF2319 0.41 -0.3 371,794 + GFF2319 0.41 +0.5 371,794 + GFF2319 0.41 -0.3 371,794 + GFF2319 0.41 -0.4 371,795 - GFF2319 0.41 +1.2 371,795 - GFF2319 0.41 -0.5 371,795 - GFF2319 0.41 +0.1 371,795 - GFF2319 0.41 -0.7 371,795 - GFF2319 0.41 -1.2 371,795 - GFF2319 0.41 +0.1 371,795 - GFF2319 0.41 +0.5 371,795 - GFF2319 0.41 +0.3 371,923 + GFF2319 0.61 -1.5 371,923 + GFF2319 0.61 -0.1 371,923 + GFF2319 0.61 -0.6 371,923 + GFF2319 0.61 +0.1 371,924 - GFF2319 0.61 -0.0 371,924 - GFF2319 0.61 -0.6 371,924 - GFF2319 0.61 -0.3 372,186 + -0.4 372,187 - +0.2 372,187 - +0.0 372,187 - -0.5 372,663 + GFF2320 0.72 +0.4 372,663 + GFF2320 0.72 +0.6 372,664 - GFF2320 0.72 +0.1 372,718 - GFF2320 0.80 +0.2 372,718 - GFF2320 0.80 +3.3 372,718 - GFF2320 0.80 +1.1 372,718 - GFF2320 0.80 +0.1 372,843 + +1.0 372,843 + +0.4 372,843 + -0.1 372,844 - -0.4 372,844 - +0.9 372,844 - -0.9 373,390 + +1.0 373,390 + +0.1 373,390 + -0.3 373,391 - -0.2 373,391 - +0.4 373,391 - -0.0 373,391 - -0.3 373,602 + GFF2322 0.31 -0.6 373,602 + GFF2322 0.31 -0.9 373,603 - GFF2322 0.31 +0.2 373,687 + GFF2322 0.43 -0.1 373,687 + GFF2322 0.43 -1.7 373,687 + GFF2322 0.43 +0.0 373,687 + GFF2322 0.43 +0.6 373,687 + GFF2322 0.43 -0.0 373,688 - GFF2322 0.44 -1.0 373,688 - GFF2322 0.44 -0.9 373,780 + GFF2322 0.57 +0.1 373,780 + GFF2322 0.57 +0.8 373,780 + GFF2322 0.57 +1.7 373,780 + GFF2322 0.57 -0.5 373,780 + GFF2322 0.57 +0.3 373,780 + GFF2322 0.57 -0.6 373,780 + GFF2322 0.57 +1.2 373,781 - GFF2322 0.58 -0.5 373,781 - GFF2322 0.58 +0.9 373,781 - GFF2322 0.58 -0.8 373,781 - GFF2322 0.58 -0.7 373,781 - GFF2322 0.58 -1.5 373,789 + GFF2322 0.59 +0.0 373,790 - GFF2322 0.59 +1.2 373,790 - GFF2322 0.59 +1.7 373,790 - GFF2322 0.59 +1.1 373,790 - GFF2322 0.59 -0.8 373,790 - GFF2322 0.59 -0.4
Or see this region's nucleotide sequence