Strain Fitness in Variovorax sp. SCN45 around GFF2251

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2250 and GFF2251 are separated by 55 nucleotidesGFF2251 and GFF2252 are separated by 16 nucleotides GFF2250 - Putative stomatin/prohibitin-family membrane protease subunit aq_911, at 306,503 to 307,654 GFF2250 GFF2251 - no description, at 307,710 to 308,204 GFF2251 GFF2252 - RNA-2',3'-PO4:RNA-5'-OH ligase, at 308,221 to 309,456 GFF2252 Position (kb) 307 308 309Strain fitness (log2 ratio) -2 -1 0 1at 306.723 kb on + strand, within GFF2250at 306.812 kb on + strand, within GFF2250at 306.813 kb on - strand, within GFF2250at 307.220 kb on + strand, within GFF2250at 307.749 kb on + strandat 307.824 kb on + strand, within GFF2251at 307.962 kb on + strand, within GFF2251at 308.051 kb on + strand, within GFF2251at 308.160 kb on + strandat 308.160 kb on + strandat 308.184 kb on + strandat 308.184 kb on + strandat 308.185 kb on - strandat 308.185 kb on - strandat 308.185 kb on - strandat 308.245 kb on + strandat 308.245 kb on + strandat 308.245 kb on + strandat 308.936 kb on - strand, within GFF2252at 309.076 kb on + strand, within GFF2252at 309.076 kb on + strand, within GFF2252at 309.076 kb on + strand, within GFF2252at 309.076 kb on + strand, within GFF2252at 309.077 kb on - strand, within GFF2252

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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306,723 + GFF2250 0.19 -0.3
306,812 + GFF2250 0.27 -1.3
306,813 - GFF2250 0.27 +0.7
307,220 + GFF2250 0.62 -0.4
307,749 + -1.0
307,824 + GFF2251 0.23 -0.6
307,962 + GFF2251 0.51 -2.1
308,051 + GFF2251 0.69 -0.8
308,160 + -0.6
308,160 + +1.2
308,184 + -0.5
308,184 + -0.1
308,185 - -0.4
308,185 - -2.0
308,185 - -0.5
308,245 + -0.0
308,245 + +0.6
308,245 + -0.3
308,936 - GFF2252 0.58 -1.4
309,076 + GFF2252 0.69 -0.6
309,076 + GFF2252 0.69 -0.3
309,076 + GFF2252 0.69 -0.5
309,076 + GFF2252 0.69 -1.3
309,077 - GFF2252 0.69 +1.7

Or see this region's nucleotide sequence